LOCUS       NM_031663               2871 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Rattus norvegicus solute carrier family 18 member A3 (Slc18a3),
            mRNA.
ACCESSION   NM_031663
VERSION     NM_031663.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Crews,F.T., Fisher,R.P., Qin,L. and Vetreno,R.P.
  TITLE     HMGB1 neuroimmune signaling and REST-G9a gene repression contribute
            to ethanol-induced reversible suppression of the cholinergic neuron
            phenotype
  JOURNAL   Mol Psychiatry 28 (12), 5159-5172 (2023)
   PUBMED   37402853
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Corsetti,V., Perrone-Capano,C., Salazar Intriago,M.S.,
            Botticelli,E., Poiana,G., Augusti-Tocco,G., Biagioni,S. and
            Tata,A.M.
  TITLE     Expression of Cholinergic Markers and Characterization of Splice
            Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons
  JOURNAL   Int J Mol Sci 22 (11), 5499 (2021)
   PUBMED   34071104
  REMARK    GeneRIF: Expression of Cholinergic Markers and Characterization of
            Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia
            Neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2871)
  AUTHORS   Szutowicz,A., Bielarczyk,H., Jankowska-Kulawy,A., Pawelczyk,T. and
            Ronowska,A.
  TITLE     Acetyl-CoA the key factor for survival or death of cholinergic
            neurons in course of neurodegenerative diseases
  JOURNAL   Neurochem Res 38 (8), 1523-1542 (2013)
   PUBMED   23677775
  REMARK    Review article
REFERENCE   4  (bases 1 to 2871)
  AUTHORS   Petrov,A.M., Naumenko,N.V., Uzinskaya,K.V., Giniatullin,A.R.,
            Urazaev,A.K. and Zefirov,A.L.
  TITLE     Increased non-quantal release of acetylcholine after inhibition of
            endocytosis by methyl-beta-cyclodextrin: the role of vesicular
            acetylcholine transporter
  JOURNAL   Neuroscience 186, 1-12 (2011)
   PUBMED   21557989
  REMARK    GeneRIF: VACHT is inserted into the presynaptic membrane during
            synaptic vesicles' exocytosis, removed during their endocytotic
            recycling, and plays a role in non-quantal secretion of
            acetylcholine.
            Erratum:[Neuroscience. 2011 Sep 29;192:806]
REFERENCE   5  (bases 1 to 2871)
  AUTHORS   Krasteva,G., Canning,B.J., Hartmann,P., Veres,T.Z., Papadakis,T.,
            Muhlfeld,C., Schliecker,K., Tallini,Y.N., Braun,A., Hackstein,H.,
            Baal,N., Weihe,E., Schutz,B., Kotlikoff,M., Ibanez-Tallon,I. and
            Kummer,W.
  TITLE     Cholinergic chemosensory cells in the trachea regulate breathing
  JOURNAL   Proc Natl Acad Sci U S A 108 (23), 9478-9483 (2011)
   PUBMED   21606356
REFERENCE   6  (bases 1 to 2871)
  AUTHORS   Cervini,R., Houhou,L., Pradat,P.F., Bejanin,S., Mallet,J. and
            Berrard,S.
  TITLE     Specific vesicular acetylcholine transporter promoters lie within
            the first intron of the rat choline acetyltransferase gene
  JOURNAL   J Biol Chem 270 (42), 24654-24657 (1995)
   PUBMED   7559575
REFERENCE   7  (bases 1 to 2871)
  AUTHORS   Roghani,A., Feldman,J., Kohan,S.A., Shirzadi,A., Gundersen,C.B.,
            Brecha,N. and Edwards,R.H.
  TITLE     Molecular cloning of a putative vesicular transporter for
            acetylcholine
  JOURNAL   Proc Natl Acad Sci U S A 91 (22), 10620-10624 (1994)
   PUBMED   7938002
REFERENCE   8  (bases 1 to 2871)
  AUTHORS   Bejanin,S., Cervini,R., Mallet,J. and Berrard,S.
  TITLE     A unique gene organization for two cholinergic markers, choline
            acetyltransferase and a putative vesicular transporter of
            acetylcholine
  JOURNAL   J Biol Chem 269 (35), 21944-21947 (1994)
   PUBMED   8071313
REFERENCE   9  (bases 1 to 2871)
  AUTHORS   Erickson,J.D., Varoqui,H., Schafer,M.K., Modi,W., Diebler,M.F.,
            Weihe,E., Rand,J., Eiden,L.E., Bonner,T.I. and Usdin,T.B.
  TITLE     Functional identification of a vesicular acetylcholine transporter
            and its expression from a 'cholinergic' gene locus
  JOURNAL   J Biol Chem 269 (35), 21929-21932 (1994)
   PUBMED   8071310
REFERENCE   10 (bases 1 to 2871)
  AUTHORS   Carroll,P.T.
  TITLE     The effect of the acetylcholine transport blocker
            2-(4-phenylpiperidino) cyclohexanol (AH5183) on the subcellular
            storage and release of acetylcholine in mouse brain
  JOURNAL   Brain Res 358 (1-2), 200-209 (1985)
   PUBMED   4075114
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U09211.1, U09838.1 and CB693073.1.
            
            On Nov 23, 2005 this sequence version replaced NM_031663.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: U09211.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2534              U09211.1           1-2534
            2535-2543           U09838.1           1792-1800
            2544-2871           CB693073.1         17-344
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p16"
     gene            1..2871
                     /gene="Slc18a3"
                     /gene_synonym="rVAT; VACht"
                     /note="solute carrier family 18 member A3"
                     /db_xref="GeneID:60422"
                     /db_xref="RGD:62072"
     exon            1..2862
                     /gene="Slc18a3"
                     /gene_synonym="rVAT; VACht"
                     /inference="alignment:Splign:2.1.0"
     CDS             859..2451
                     /gene="Slc18a3"
                     /gene_synonym="rVAT; VACht"
                     /note="solute carrier family 18 (vesicular acetylcholine),
                     member 3; solute carrier family 18, member 3; solute
                     carrier family 18 (vesicular monoamine) member 3; solute
                     carrier family 18 (vesicular acetylcholine transporter),
                     member 3"
                     /codon_start=1
                     /product="vesicular acetylcholine transporter"
                     /protein_id="NP_113851.1"
                     /db_xref="GeneID:60422"
                     /db_xref="RGD:62072"
                     /translation="MEPTAPTGQARAAATKLSEAVGAALQEPQRQRRLVLVIVCVALL
                     LDNMLYMVIVPIVPDYIAHMRGGSEGPTLVSEVWEPTLPPPTLANASAYLANTSASPT
                     AAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLG
                     VMFASTVMFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVAL
                     AFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANL
                     PVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPA
                     FVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCF
                     GIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQ
                     LSLGMGLANLLYAPVLLLLRNVGLLTRSRSERDVLLDEPPQGLYDAVRLREVQGKDGG
                     EPCSPPGPFDGCEDDYNYYSRS"
ORIGIN      
        1 ggcagcgcgg ctcacctcgg gggctccgtg cccgctgtgc gccgaagtcc aggctgagga
       61 ggaggtctag agcccccggc tctcccgtct cccaccaggc tgcggggaac tggctgccgc
      121 acccctcctc caagtggggg tagaacggag tctcaccccc ataggtccca gaactaaggg
      181 gaacataggg ccggttcctc ccactgctca gccatcccca ggggcttgtc taggactata
      241 gctctccaaa tcccccttcc cctggcttcc atcctgggcg catctcagaa gcggacccct
      301 gcccggacgc gccccgcccc cggcccccgc cccgacgacg tcctattagc atgagcgacg
      361 ccagtggccg gggcaccact cgggggccga gactcaccgc gtcatagccc caagtggagg
      421 gagaaagaaa aaaaaagagg cggcggtgga ggaagaggca agagcggacg cgcggggagg
      481 gctggagaga cggcgggcgg cggcagcatg cccctgggcg ggtgcacacg gcctctctgc
      541 accgcagggg ctgcttctgc tctctctggg caccacgcgt ccagtctccc gcctcagccc
      601 ctcggcttgc cggcctttgc ggttgcgctc gaaacatcgt ccactggtcc ccgaagcatc
      661 taagagcagc ggcgccgcgc gggacaatcc ttgctttttt ctgagctcgg ggatatgagc
      721 cccacagcca cctgaagcgc agggggcgct acgcggctag gaccgcgccc ccgaagtacc
      781 ttatcctagc ctctgcactg cgggacgccg acacccgact ccggtggagg catcttaggg
      841 aaagcagccg gtaggggcat ggaacccacc gcgccaaccg gtcaggcccg ggcggcggcc
      901 accaaactgt cggaagcggt gggagccgcg ctacaagagc cccagaggca gcggcgcctg
      961 gtgctggtca tcgtgtgcgt tgcactgtta ctggacaaca tgttgtacat ggtcatcgtg
     1021 cccattgttc ccgactatat cgcccacatg cgcgggggca gcgagggccc gaccctggtc
     1081 tctgaggtgt gggaacccac tctgccgccg cccactctgg ctaatgccag tgcctacttg
     1141 gccaacacgt cggcgtcccc gacggctgcc gggtcggctc ggtcaatcct gcgacctcgc
     1201 taccccacag aaagcgaaga tgtgaagata ggtgtgctgt ttgcctccaa ggctatcctg
     1261 cagcttctgg tgaacccctt aagcgggcct ttcattgatc gcatgagcta cgacgtgccg
     1321 ctgcttatag gcctgggcgt catgttcgcc tccacagtca tgtttgcctt tgcagaagac
     1381 tatgccacgc tcttcgctgc gcgcagtcta caaggcctgg gctcggcctt cgcggacacg
     1441 tctggcattg ccatgatcgc cgacaagtat cccgaggagc ctgagcgcag tcgtgccctg
     1501 ggcgtggcgc tagcctttat tagctttgga agcctagtgg cgccaccgtt tgggggcatc
     1561 ctctacgagt tcgcgggcaa gcgtgtaccc tttctagtgc tcgccgctgt gtcccttttc
     1621 gacgcgctcc tgctcctggc ggtggctaag cccttctcgg ctgcggctcg ggcgcgagcc
     1681 aacctgccgg tgggcacacc tatccatcgc ctcatgctag acccttacat cgctgtggta
     1741 gccggcgcgc tcaccacttg taacattccc cttgcgttcc tcgagcccac catagccacg
     1801 tggatgaagc acacaatggc cgcatccgag tgggagatgg gcatggtttg gctgccggct
     1861 ttcgtgccac acgtgttagg cgtctacctc accgtgcgcc tggcggcgcg ttatccacac
     1921 ctgcagtggc tgtacggcgc tctcgggcta gcggtaattg gagtgagctc ttgcgtcgta
     1981 cctgcctgtc gctcattcgc gccgttagtg gtctcgctct gcggactctg cttcggcatc
     2041 gcgttagtgg acacagcgct cctacccacg ctcgcctttc tggtggacgt gcgccacgta
     2101 tccgtctatg gcagtgtcta tgccatagct gacatctcct attctgtggc ctacgcgctc
     2161 gggcccatag tggcaggcca catcgttcac tctcttggct ttgagcagct cagcctgggc
     2221 atgggcctgg ccaacctgct ctacgcacca gtccttcttc ttttgcgcaa tgtaggcctc
     2281 cttacacgct cgcgttcgga gcgcgatgtg ttgcttgatg aaccgccgca gggtctgtac
     2341 gacgcggtgc gcctgcgtga ggtgcagggc aaggatggcg gcgaaccttg tagcccacct
     2401 ggcccttttg acgggtgcga ggacgactac aactattact cccgcagcta gcagacccgc
     2461 ttctcctcca ggccacctac ccgccccatt taggtcaaga tggtcattct gcaagagcac
     2521 tgtccaactt tggcctgggg cccacctcct ctaatgaata ccctagcccc tcgcccgtcc
     2581 tgaattcctt tgctggaatc ccttctccat gacccctccc agtctaggcc cctccccaaa
     2641 cacactcgta ttcattgggg aaatggagca gggaggcaga agaagctgtt gggctcttgg
     2701 cagaggtgaa gaggtgtgcg ggtgatcgcc aatcacctac tgagagcccc caaatagagt
     2761 catgcatctg tttgtccttc ctgcggatct ttccagtgcc aaacttggtc tctgcactcc
     2821 ggtgcctccg gcctgaatta ataaaccata tctatctgag gaaaaaaaaa a
//
