LOCUS       NM_019347               3965 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus solute carrier family 14 member 2 (Slc14a2),
            transcript variant 1, mRNA.
ACCESSION   NM_019347 XM_579434
VERSION     NM_019347.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Chou,C.L., Hwang,G., Hageman,D.J., Han,L., Agrawal,P., Pisitkun,T.
            and Knepper,M.A.
  TITLE     Identification of UT-A1- and AQP2-interacting proteins in rat inner
            medullary collecting duct
  JOURNAL   Am J Physiol Cell Physiol 314 (1), C99-C117 (2018)
   PUBMED   29046292
  REMARK    GeneRIF: UT-A1 and AQP2 interactomes provide a snapshot of a
            dynamic process in which UT-A1 and AQP2 are produced in the rough
            endoplasmic reticulum, processed through the Golgi apparatus,
            delivered to endosomes that move into and out of the plasma
            membrane, and are regulated in the plasma membrane.
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Nawata,C.M., Dantzler,W.H. and Pannabecker,T.L.
  TITLE     Alternative channels for urea in the inner medulla of the rat
            kidney
  JOURNAL   Am J Physiol Renal Physiol 309 (11), F916-F924 (2015)
   PUBMED   26423860
  REMARK    GeneRIF: Data indicate that there are several alternative channels
            for urea in the rat inner medulla that could potentially contribute
            to the high urea permeabilities in thin limb segments.
REFERENCE   3  (bases 1 to 3965)
  AUTHORS   Feng,X., Li,Z., Du,Y., Fu,H., Klein,J.D., Cai,H., Sands,J.M. and
            Chen,G.
  TITLE     Downregulation of urea transporter UT-A1 activity by 14-3-3 protein
  JOURNAL   Am J Physiol Renal Physiol 309 (1), F71-F78 (2015)
   PUBMED   25995111
  REMARK    GeneRIF: Activation of cAMP/PKA increases 14-3-3gamma interactions
            with UT-A1 and stimulates MDM2-mediated UT-A1 ubiquitination and
            degradation, forming a novel regulatory mechanism of urea transport
            activity.
REFERENCE   4  (bases 1 to 3965)
  AUTHORS   Esteva-Font,C., Phuan,P.W., Lee,S., Su,T., Anderson,M.O. and
            Verkman,A.S.
  TITLE     Structure-activity analysis of thiourea analogs as inhibitors of
            UT-A and UT-B urea transporters
  JOURNAL   Biochim Biophys Acta 1848 (5), 1075-1080 (2015)
   PUBMED   25613743
  REMARK    GeneRIF: Report thiourea analogs as inhibitors of UT-A mediated
            urea transport.
REFERENCE   5  (bases 1 to 3965)
  AUTHORS   Hoban,C.A., Black,L.N., Ordas,R.J., Gumina,D.L., Pulous,F.E.,
            Sim,J.H., Sands,J.M. and Blount,M.A.
  TITLE     Vasopressin regulation of multisite phosphorylation of UT-A1 in the
            inner medullary collecting duct
  JOURNAL   Am J Physiol Renal Physiol 308 (1), F49-F55 (2015)
   PUBMED   25377918
  REMARK    GeneRIF: UT-A1 localized to the apical membrane in response to
            vasopressin was phosphorylated at Ser(486) and Ser(499).
REFERENCE   6  (bases 1 to 3965)
  AUTHORS   Shayakul,C., Tsukaguchi,H., Berger,U.V. and Hediger,M.A.
  TITLE     Molecular characterization of a novel urea transporter from kidney
            inner medullary collecting ducts
  JOURNAL   Am J Physiol Renal Physiol 280 (3), F487-F494 (2001)
   PUBMED   11181411
REFERENCE   7  (bases 1 to 3965)
  AUTHORS   Fenton,R.A., Howorth,A., Cooper,G.J., Meccariello,R., Morris,I.D.
            and Smith,C.P.
  TITLE     Molecular characterization of a novel UT-A urea transporter isoform
            (UT-A5) in testis
  JOURNAL   Am J Physiol Cell Physiol 279 (5), C1425-C1431 (2000)
   PUBMED   11029290
REFERENCE   8  (bases 1 to 3965)
  AUTHORS   Karakashian,A., Timmer,R.T., Klein,J.D., Gunn,R.B., Sands,J.M. and
            Bagnasco,S.M.
  TITLE     Cloning and characterization of two new isoforms of the rat kidney
            urea transporter: UT-A3 and UT-A4
  JOURNAL   J Am Soc Nephrol 10 (2), 230-237 (1999)
   PUBMED   10215321
REFERENCE   9  (bases 1 to 3965)
  AUTHORS   Shayakul,C., Steel,A. and Hediger,M.A.
  TITLE     Molecular cloning and characterization of the vasopressin-regulated
            urea transporter of rat kidney collecting ducts
  JOURNAL   J Clin Invest 98 (11), 2580-2587 (1996)
   PUBMED   8958221
REFERENCE   10 (bases 1 to 3965)
  AUTHORS   Smith,C.P., Lee,W.S., Martial,S., Knepper,M.A., You,G., Sands,J.M.
            and Hediger,M.A.
  TITLE     Cloning and regulation of expression of the rat kidney urea
            transporter (rUT2)
  JOURNAL   J Clin Invest 96 (3), 1556-1563 (1995)
   PUBMED   7657826
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF031642.1, AF041788.1, U77971.1 and CK844529.1.
            
            [WARNING] On Apr 9, 2024 this sequence was replaced by NM_019347.4.
            
            On Feb 26, 2021 this sequence version replaced NM_019347.2.
            
            Transcript Variant: This variant (1), also known as UT1, UT-A1, or
            VRUT, represents the longest transcript and encodes the longest
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U77971.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMD00155332,
                                           SAMEA5756307 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-425               AF031642.1         1-425
            426-664             AF041788.1         426-664
            665-3961            U77971.1           665-3961
            3962-3965           CK844529.1         19-22               c
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.3"
     gene            1..3965
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /note="solute carrier family 14 member 2"
                     /db_xref="GeneID:54302"
                     /db_xref="RGD:3689"
     exon            1..169
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            170..300
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            301..365
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            366..550
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..3166
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /note="isoform 1 is encoded by transcript variant 1; urea
                     transporter, kidney; urea transporter 2; urea transport
                     protein; plasma membrane urea transporter; testName;
                     solute carrier family 14 (urea transporter), member 2T;
                     urea transporter UT-A2c; urea transporter UT-A2d"
                     /codon_start=1
                     /product="urea transporter 2 isoform 1"
                     /protein_id="NP_062220.2"
                     /db_xref="GeneID:54302"
                     /db_xref="RGD:3689"
                     /translation="MSDHPLKEMSDNNRSPLLPEPLSSRYKLYESELSSPTWPSSSQD
                     THPALPLLEMPEEKDLRSSDEDSHIVKIEKPNERSKRRESELPRRASAGRGGFSLFQA
                     VSYLTGDMKECKNWLKDKPLVLQFLDWVLRGAAQVMFVNNPLSGLIIFIGLLIQNPWW
                     TIAGALGTVVSTLAALALSQDRSAIASGLHGYNGMLVGLLVAVFSEKLDYYWWLLFPV
                     TFASMACPVISSALSTVFAKWDLPVFTLPFNIALTLYLAATGHYNLFFPTTLVKPASS
                     APNITWSEIEMPLLLQTIPVGVGQVYGCDNPWTGGVILVALFISSPLICLHAAIGSIV
                     GLLAALTVATPFETIYTGLWSYNCVLSCVAIGGMFYVLTWQTHLLALVCALFCAYTGA
                     ALSNMMAVVGVPPGTWAFCLSTLTFLLLTSNNPGIHKLPLSKVTYPEANRIYFLTAKR
                     SDEQKPPNGGGGEQSHGGGQRKAEEGSETVFPRRKSVFHIEWSSIRRRSKVFGKSEHQ
                     ERQTKEPLPYLYRKPTVELLDLNTMEESSEIKVETNTTRTTWIQSSMIAGGKRVSKAL
                     SYITGEMKECGEGLKDKSPVFQFLDWVLRGTSQVMFVNNPLSGILIVLGLFVQNPWWA
                     ISGCLGTIMSTLTALILSQDKSAIAAGLHGYNGVLVGLLMAVFSDKGNYYWWLLLPVI
                     VMSMTCPILSSALSTVFSKWDLPVFTLPFNIAVTLYLAATGHYNLFFPTKLLQPAVTT
                     PNITWSDVQVPLLLRAIPVGIGQVYGCDNPWTGGIFLVALFVSSPLICLHAAIGSTIG
                     MLAALSIATPFDSIYFGLCGFNSTLACIAIGGMFYVITWQTHLLAIACALFAAYLGAA
                     LANMLSVFGLPPCTWPFCLSALTFLLLTTNNPGIYKLPLSKVTYPEANRIYFLSQEKN
                     RRASMITKYQAYDVS"
     exon            551..731
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            732..921
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1050
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1243
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1391
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1526
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1576
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1751
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1877
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1960
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2120
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..2310
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2439
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2632
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2780
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..2915
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2916..2965
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
     exon            2966..3965
                     /gene="Slc14a2"
                     /gene_synonym="Slc14a2T; Slc14a2_v4; testSymbol; UrT1-C;
                     UrT1-D"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaaaagagga aagacagagc cggcctagac agcagacatc aggacaccct tctaggccag
       61 ctctgttttc tgccttaatg ctcaaggaag actgcaagat catctgaggt gggctctgta
      121 acagcgtcag ctccacaaag tcatcgctgc aaagttcagg agatgggaga tctttcctgt
      181 tttcttactg agtgtttccg ggtgtgagtg catcgcattg acatcttcct acctctgtgg
      241 acaaacaagg acaaggctcg aatcccctca gtctacttca tcagccggac ctgcatgact
      301 ggctattttg accacttgaa gtcatctaaa tagaagttct caagaccacc aagaccaagg
      361 acacttcaca tgacagatga gcgaccatcc cctgaaggag atgtctgaca acaaccgcag
      421 ccccctcttg ccagagcctc tttccagcag atacaagttg tatgagtcag agctaagcag
      481 ccctacctgg ccttcaagct cccaggatac acacccagcc cttcccctgc tggaaatgcc
      541 tgaagaaaag gatctccgat catcggatga agacagccac atagtgaaga ttgaaaagcc
      601 caacgagcgg agtaaacgga gagaaagcga gttgccccgc agggcttctg caggccgggg
      661 aggcttcagc ctcttccaag ctgtgagcta cctcactggt gacatgaagg aatgcaagaa
      721 ctggctgaaa gataagcccc tggtccttca gttcctagac tgggtcctgc ggggagcagc
      781 tcaggtgatg tttgtcaaca acccccttag tgggctcatc atcttcatag ggctcctgat
      841 ccagaatccc tggtggacga ttgctggggc actggggaca gtggtctcaa ccttggctgc
      901 ccttgccttg agccaggaca ggtcagccat cgcctctgga ctccatgggt acaacgggat
      961 gctggtggga ctcctggtgg ctgtcttctc agagaagtta gactactact ggtggcttct
     1021 gtttcctgtg accttcgcat ccatggcctg cccagttatt tctagtgcct tgagcaccgt
     1081 cttcgccaag tgggacctgc cagtcttcac actgcccttc aacatcgcct taacactgta
     1141 cctggcagct acgggccact acaacctttt cttccccacg acgcttgtga agcctgcgtc
     1201 ttcagcgccc aacatcacct ggtcagagat tgagatgcct ttgctgttac aaaccatccc
     1261 cgttggggtg ggccaggtct acggctgtga caacccctgg acaggcggcg tgatcctggt
     1321 ggctctgttt atctcctctc ccctcatctg tttgcacgca gccataggat ccattgtggg
     1381 gctgctggca gcactgacgg tggccactcc cttcgagacg atctacacgg gcctttggag
     1441 ctacaactgt gtcctctcct gtgtcgccat tggaggcatg ttctacgtac tcacctggca
     1501 gacacacctg ttggcacttg tctgtgccct gttctgtgcg tacacgggag cggccctctc
     1561 caatatgatg gcagtggttg gtgtgccacc gggcacctgg gccttctgtc tctccaccct
     1621 caccttcctt ctcctcacaa gcaacaaccc tggcatccac aagctcccac tcagcaaagt
     1681 cacctaccca gaggccaacc gcatctactt cctgacagcg aaacgcagtg acgagcagaa
     1741 gccccccaat ggtggcggtg gagaacagtc acacggagga ggccagcgga aggcagagga
     1801 gggctccgag accgtgttcc ccaggcgtaa gagcgtgttc catatcgagt ggtcatccat
     1861 ccggaggagg agcaaagtgt ttggaaaaag tgaacaccaa gagagacaaa ccaaagagcc
     1921 actcccctac ctgtatcgga agcccacagt ggaactcttg gacctgaaca ccatggagga
     1981 gagctctgag ataaaggtag aaaccaacac aaccaggact acctggatcc agagctccat
     2041 gatcgctggg gggaagagag tcagcaaagc cctcagctac atcacaggag agatgaagga
     2101 gtgcggcgag ggacttaaag acaaatctcc agtgttccag tttcttgact gggtgctccg
     2161 aggcacgtct caggtgatgt ttgtgaacaa ccctctcagt ggcatcctca tcgttcttgg
     2221 cctcttcgtg cagaatccct ggtgggccat ctccggttgc ttgggcacca tcatgtccac
     2281 cttgactgcc ctcatcctga gccaggacaa gtcggccatt gcagcaggac tccatggcta
     2341 caatggggtg cttgtggggc tcctgatggc cgtgttctca gacaagggca attactactg
     2401 gtggctgctg ctcccggtca tcgtcatgtc catgacttgc cccatcctct ccagtgctct
     2461 gagcaccgtc ttcagcaaat gggacctccc agtcttcaca ctgcccttca acatcgccgt
     2521 gaccctgtac ctggcagcca cgggccacta caatctcttc ttccccacga agctgctgca
     2581 gcctgcagtt accacaccca acatcacctg gtcagatgtc caagtgcctt tgcttctgag
     2641 agccatcccc gttggaatcg gccaagtgta tggctgtgac aatccctgga ctggaggcat
     2701 cttcctcgtt gctctgttcg tctcttcacc tctcatctgc ttgcacgctg ccatcggatc
     2761 cacgataggg atgttagcag cactcagcat tgccacacca tttgactcca tctactttgg
     2821 cctgtgtggc ttcaatagca ctctggcctg tatcgctatt ggtggcatgt tctacgtcat
     2881 cacctggcag acgcacctgc tcgccattgc ctgtgccctg tttgcagcct acctgggtgc
     2941 tgccctggcc aacatgctgt ctgtgtttgg attaccaccc tgcacctggc ccttctgcct
     3001 ctcagcgctc accttcctcc tcctcacaac caacaaccct ggcatctaca agctcccgct
     3061 cagcaaagtc acctacccag aggccaaccg catctacttc ctgtcccagg agaaaaacag
     3121 aagggcatcg atgataacaa agtaccaggc ctacgacgtc tcctaagctg ccctttctaa
     3181 gctcgtctgg taaatgcagc ctccagaacc caccctgcac cccaggctaa atctaagtca
     3241 ctcaaccacc cttcttccta atatgcgagt ggctgctatt tggagccctc aatgagatgt
     3301 gcagcgctca tcaaagccac gctagtttaa actaaatacc caccgatggc tctttgggaa
     3361 ctctctgtgg ggggaacttg ctagtcacct ctttcagtta tttagtggcg caaatcatga
     3421 ttccaggaaa tgttcattag cctgccatgc aagagggctg ggttcactaa tgagttccaa
     3481 ctgtctcagg ccagtcttct ccctacccca tccccacccc tatccacacc caccccaccc
     3541 ctcatctcag cctgggtgtt ttgttgccaa ggatttcttt accatgacaa ggaaggactc
     3601 caaagtcctt ccacctcaga agcatttatt cttttccata tcctgtaaac taaccaactc
     3661 agcatggcca gcaagtaatt attctgactt gagttactta tggaatggct ctcagaggag
     3721 agaggaaaat gttagtgccc tgtggaagtt tattcttaga tatctgggag atgctctaag
     3781 gcttgggacc ggacacctga attcattgaa aatgccaatg tatcttcttt gcaccataga
     3841 aatatttcca gaaacatcat ctggtaagga gtgacctttt ccaacatcca atcatagtgt
     3901 aagcaaacgt ctttgcttgt gtaatattca aaatcagtag tcattaaact tgaaatttgc
     3961 acgtt
//
