LOCUS       NM_134363               5693 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Rattus norvegicus solute carrier family 12 member 5 (Slc12a5),
            transcript variant 2, mRNA.
ACCESSION   NM_134363
VERSION     NM_134363.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Hamze,M., Brier,C., Buhler,E., Zhang,J., Medina,I. and Porcher,C.
  TITLE     Regulation of Neuronal Chloride Homeostasis by Pro- and Mature
            Brain-Derived Neurotrophic Factor (BDNF) via KCC2 Cation-Chloride
            Cotransporters in Rat Cortical Neurons
  JOURNAL   Int J Mol Sci 25 (11), 6253 (2024)
   PUBMED   38892438
  REMARK    GeneRIF: Regulation of Neuronal Chloride Homeostasis by Pro- and
            Mature Brain-Derived Neurotrophic Factor (BDNF) via KCC2
            Cation-Chloride Cotransporters in Rat Cortical Neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Ma,Y., Chen,J., Chen,C., Wei,B. and Liu,X.
  TITLE     Suppression of HCN channels in the spinal dorsal horn restores KCC2
            expression and attenuates diabetic neuropathic pain
  JOURNAL   Neurosci Lett 822, 137626 (2024)
   PUBMED   38191090
  REMARK    GeneRIF: Suppression of HCN channels in the spinal dorsal horn
            restores KCC2 expression and attenuates diabetic neuropathic pain.
REFERENCE   3  (bases 1 to 5693)
  AUTHORS   Pol,E., Come,E., Merlaud,Z., Gouhier,J., Russeau,M.,
            Scotto-Lomassese,S., Moutkine,I., Marques,X. and Levi,S.
  TITLE     NKCC1 and KCC2 Chloride Transporters Have Different Membrane
            Dynamics on the Surface of Hippocampal Neurons
  JOURNAL   Cells 12 (19), 2363 (2023)
   PUBMED   37830575
  REMARK    GeneRIF: NKCC1 and KCC2 Chloride Transporters Have Different
            Membrane Dynamics on the Surface of Hippocampal Neurons.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5693)
  AUTHORS   Calvo,P.M., de la Cruz,R.R., Pastor,A.M. and Alvarez,F.J.
  TITLE     Preservation of KCC2 expression in axotomized abducens motoneurons
            and its enhancement by VEGF
  JOURNAL   Brain Struct Funct 228 (3-4), 967-984 (2023)
   PUBMED   37005931
  REMARK    GeneRIF: Preservation of KCC2 expression in axotomized abducens
            motoneurons and its enhancement by VEGF.
REFERENCE   5  (bases 1 to 5693)
  AUTHORS   Haratizadeh,S., Ranjbar,M., Darvishzadeh-Mahani,F., Basiri,M. and
            Nozari,M.
  TITLE     The effects of postnatal erythropoietin and nano-erythropoietin on
            behavioral alterations by mediating K-Cl co-transporter 2 in the
            valproic acid-induced rat model of autism
  JOURNAL   Dev Psychobiol 65 (1), e22353 (2023)
   PUBMED   36567653
  REMARK    GeneRIF: The effects of postnatal erythropoietin and
            nano-erythropoietin on behavioral alterations by mediating K-Cl
            co-transporter 2 in the valproic acid-induced rat model of autism.
REFERENCE   6  (bases 1 to 5693)
  AUTHORS   Strange,K., Singer,T.D., Morrison,R. and Delpire,E.
  TITLE     Dependence of KCC2 K-Cl cotransporter activity on a conserved
            carboxy terminus tyrosine residue
  JOURNAL   Am J Physiol Cell Physiol 279 (3), C860-C867 (2000)
   PUBMED   10942735
REFERENCE   7  (bases 1 to 5693)
  AUTHORS   Rivera,C., Voipio,J., Payne,J.A., Ruusuvuori,E., Lahtinen,H.,
            Lamsa,K., Pirvola,U., Saarma,M. and Kaila,K.
  TITLE     The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during
            neuronal maturation
  JOURNAL   Nature 397 (6716), 251-255 (1999)
   PUBMED   9930699
REFERENCE   8  (bases 1 to 5693)
  AUTHORS   Payne,J.A.
  TITLE     Functional characterization of the neuronal-specific K-Cl
            cotransporter: implications for [K+]o regulation
  JOURNAL   Am J Physiol 273 (5), C1516-C1525 (1997)
   PUBMED   9374636
REFERENCE   9  (bases 1 to 5693)
  AUTHORS   Payne,J.A., Stevenson,T.J. and Donaldson,L.F.
  TITLE     Molecular characterization of a putative K-Cl cotransporter in rat
            brain. A neuronal-specific isoform
  JOURNAL   J Biol Chem 271 (27), 16245-16252 (1996)
   PUBMED   8663311
REFERENCE   10 (bases 1 to 5693)
  AUTHORS   Gillen,C.M., Brill,S., Payne,J.A. and Forbush,B. 3rd.
  TITLE     Molecular cloning and functional expression of the K-Cl
            cotransporter from rabbit, rat, and human. A new member of the
            cation-chloride cotransporter family
  JOURNAL   J Biol Chem 271 (27), 16237-16244 (1996)
   PUBMED   8663127
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Mar 2, 2021 this sequence version replaced NM_134363.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMEA5760433 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               JAXUCZ010000003.1  174123215-174123373
            160-254             JAXUCZ010000003.1  174128584-174128678
            255-386             JAXUCZ010000003.1  174129003-174129134
            387-533             JAXUCZ010000003.1  174129469-174129615
            534-588             JAXUCZ010000003.1  174130420-174130474
            589-719             JAXUCZ010000003.1  174130975-174131105
            720-961             JAXUCZ010000003.1  174133633-174133874
            962-1173            JAXUCZ010000003.1  174134563-174134774
            1174-1344           JAXUCZ010000003.1  174137179-174137349
            1345-1443           JAXUCZ010000003.1  174137602-174137700
            1444-1501           JAXUCZ010000003.1  174138022-174138079
            1502-1676           JAXUCZ010000003.1  174141213-174141387
            1677-1795           JAXUCZ010000003.1  174142083-174142201
            1796-1894           JAXUCZ010000003.1  174142536-174142634
            1895-2014           JAXUCZ010000003.1  174143544-174143663
            2015-2119           JAXUCZ010000003.1  174144002-174144106
            2120-2288           JAXUCZ010000003.1  174145218-174145386
            2289-2484           JAXUCZ010000003.1  174147551-174147746
            2485-2654           JAXUCZ010000003.1  174149017-174149186
            2655-2786           JAXUCZ010000003.1  174149447-174149578
            2787-2894           JAXUCZ010000003.1  174150366-174150473
            2895-3017           JAXUCZ010000003.1  174151531-174151653
            3018-3217           JAXUCZ010000003.1  174151746-174151945
            3218-3232           JAXUCZ010000003.1  174152262-174152276
            3233-3366           JAXUCZ010000003.1  174152506-174152639
            3367-5693           JAXUCZ010000003.1  174152786-174155112
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..5693
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /note="solute carrier family 12 member 5"
                     /db_xref="GeneID:171373"
                     /db_xref="RGD:620811"
     exon            1..159
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..3458
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /function="K-Cl cotransport"
                     /note="isoform 2 is encoded by transcript variant 2;
                     neuronal-specific K-Cl cotransporter; K-Cl cotransporter
                     2; neuronal K-Cl cotransporter; furosemide-sensitive K-Cl
                     cotransporter; electroneutral potassium-chloride
                     cotransporter 2; solute carrier family 12
                     (potassium-chloride transporter), member 5"
                     /codon_start=1
                     /product="solute carrier family 12 member 5 isoform 2"
                     /protein_id="NP_599190.1"
                     /db_xref="GeneID:171373"
                     /db_xref="RGD:620811"
                     /translation="MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGREYDGRN
                     MALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMG
                     VYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVP
                     AGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAED
                     ASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIK
                     SAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEY
                     FTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYV
                     FSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSV
                     VLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPR
                     LLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFL
                     MCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLI
                     AGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQ
                     DQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKG
                     FCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRET
                     TAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKM
                     RIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQ
                     ILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQ
                     LIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIK
                     DFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYM
                     EFLEVLTEQLDRVMLVRGGGREVITIYS"
     exon            160..254
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            255..386
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            387..533
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            534..588
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            589..719
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..961
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1173
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1344
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1443
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1501
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1676
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1795
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1894
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2014
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2119
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2288
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2484
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2654
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2786
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2787..2894
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            2895..3017
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3217
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3232
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3233..3366
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..5693
                     /gene="Slc12a5"
                     /gene_synonym="Kcc2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggagagcaa gcgacagagc tcgagcaagc gagcgagcgg cgaaggcggg cagaggggcg
       61 cgggcgaaga ggcgcagcca tcccgagccc ggcgccgcgc agccaccatg ctcaacaacc
      121 tgacggactg cgaggacggc gatgggggag ccaacccggg tgacggcaat cccaaggaga
      181 gcagcccctt catcaacagc acggacacgg agaaggggag agagtatgat ggcaggaaca
      241 tggccctgtt tgaggaggag atggacacca gccccatggt atcctccctg ctcagtgggc
      301 tggccaacta caccaacctg cctcagggaa gcaaagagca cgaagaagca gaaaacaatg
      361 agggcggaaa gaagaagccg gtgcaggccc cacgcatggg caccttcatg ggcgtgtacc
      421 tcccgtgcct gcagaacatc tttggtgtta tcctctttct gcggctcact tgggtggtgg
      481 gaatcgcagg catcatggag tccttctgca tggtcttcat ctgctgctcc tgcacgatgc
      541 tcacagccat ttccatgagc gcaattgcaa ccaatggtgt tgtgcctgct ggtggctcct
      601 actacatgat ttccaggtct ctgggcccgg agtttggggg cgccgtgggc ctctgcttct
      661 acctgggcac tacctttgct ggggctatgt acatcctggg caccatcgag atcctgctgg
      721 cttacctctt cccagcaatg gccatcttca aggcagaaga tgccagtggg gaggcagccg
      781 ccatgttgaa taacatgcgg gtgtatggca cctgtgtgct cacctgcatg gccaccgtag
      841 tctttgtggg cgtcaagtac gtgaacaagt ttgccctggt cttcctgggt tgcgtgatcc
      901 tctccatcct ggccatctac gcaggggtca tcaagtctgc cttcgatcca cccaatttcc
      961 cgatttgcct cctggggaac cgcacgctgt ctcgccatgg ctttgatgtc tgtgccaagc
     1021 tggcttggga aggaaatgag acggtgacca cacggctctg gggcctattc tgttcctccc
     1081 gcctcctcaa tgccacctgt gatgagtact tcacccgaaa caatgtcaca gagatccagg
     1141 gcattcctgg tgctgcaagt ggcctcatca aagagaacct gtggagttcc tacctgacca
     1201 agggggtgat cgtggagagg cgtgggatgc cctccgtggg cctggcagat ggtacccccg
     1261 ttgacatgga ccacccctat gtcttcagtg atatgacctc ctacttcacc ctgcttgttg
     1321 gcatctattt cccctcagtc acagggatca tggctggctc gaaccggtcc ggagacctgc
     1381 gggatgccca gaagtctatc cctactggaa ctatcttggc cattgctacg acctctgctg
     1441 tctacatcag ctctgttgtt ctgttcggag cctgcatcga aggggtcgtc ctacgggaca
     1501 agtttgggga agctgtgaat ggcaatctgg tggtgggcac cctggcctgg ccttctcctt
     1561 gggtcattgt cataggctct ttcttctcta cctgcggagc tggactacag agcctcacag
     1621 gggccccacg cctgctgcag gccatctccc gggatggcat agtgcccttc ctgcaggtct
     1681 ttggccatgg caaagccaac ggagagccaa cctgggcgct gctgctgact gcctgcatct
     1741 gtgagatcgg catcctcatc gcctccctgg atgaggtcgc ccctatcctt tccatgttct
     1801 tcctgatgtg ttacatgttt gtgaacttgg cttgcgcggt gcagacactg ctgaggacgc
     1861 ccaactggag gccacgtttc cgatattacc actggaccct ctccttcctg ggcatgagcc
     1921 tctgcctggc cctgatgttc atttgctcct ggtattatgc gctggtagct atgctcattg
     1981 ctggcctcat ctataagtac atcgagtacc ggggggcaga gaaggagtgg ggggatggga
     2041 tccgaggcct gtctctcagt gcagctcgct atgctctctt gcgtctggag gaaggacccc
     2101 cgcatacaaa gaactggagg ccccagctac tggtgctggt gcgtgtggac caggaccaga
     2161 acgtggtgca cccgcagctg ctgtccttga cctcccagct caaggcaggg aagggcctga
     2221 ccattgtggg ctctgtcctt gagggcacct ttctggacaa ccaccctcag gctcagcggg
     2281 cagaggagtc tatccggcgc ctgatggagg ctgagaaggt gaagggcttc tgccaggtag
     2341 tgatctcctc caacctgcgt gacggtgtgt cccacctgat ccaatccggg ggcctcgggg
     2401 gcctgcaaca caacactgtg ctagtgggct ggcctcgcaa ctggcgacag aaggaggatc
     2461 atcagacatg gaggaacttc atcgaactcg tccgggaaac tacagctggc cacctcgccc
     2521 tgctggtcac caagaatgtt tccatgttcc ccgggaaccc tgagcgtttc tctgagggca
     2581 gcattgacgt gtggtggatc gtgcacgacg ggggcatgct catgctgttg cccttcctcc
     2641 tgcgtcacca caaggtctgg aggaaatgca aaatgcggat cttcaccgtg gcgcagatgg
     2701 atgacaacag cattcagatg aagaaagacc tgaccacgtt tctgtaccac ttacgaatta
     2761 ctgcagaggt ggaagtcgtg gagatgcacg agagcgacat ctcagcatac acctacgaga
     2821 agacattggt aatggaacaa cgttctcaga tcctcaaaca gatgcacctc accaagaacg
     2881 agcgggaacg ggagatccag agcatcacag atgaatctcg gggctccatt cggaggaaga
     2941 atccagccaa cactcggctc cgcctcaatg ttcccgaaga gacagcttgt gacaacgagg
     3001 agaagccaga agaggaggtg cagctgatcc atgaccagag tgctcccagc tgccctagca
     3061 gctcgccgtc tccaggggag gagcctgagg gggaggggga gacagaccca gagaaggtgc
     3121 atctcacctg gaccaaggat aagtcagcgg ctcagaagaa caaaggcccc agtcccgtct
     3181 cctcggaggg gatcaaggac ttcttcagca tgaagccgga gtgggaaaac ttgaaccagt
     3241 ccaacgtgcg gcgcatgcac acagctgtgc ggctgaacga ggtcatcgtg aataaatccc
     3301 gggatgccaa gttggtgttg ctcaacatgc ccgggcctcc ccgcaaccgc aatggagatg
     3361 aaaactacat ggaattcctg gaggtcctca ctgagcaact ggaccgggtg atgctggtcc
     3421 gcggtggtgg ccgagaggtc atcaccatct actcctgaag gccaggacct gccactccgg
     3481 cccgagcgag cccggcccgc ggccccggag ccctcgccgc gcctccccgc cgctgtcacc
     3541 gtttacataa gaccccgttg cccgtgccct ggccctcttc cctcccgctg cctgcggccc
     3601 ggaggccttg cccgtcgggg ctgacccgga gggcggcccg tgggcccctt ttctgagccc
     3661 ggcctcgccc tgccggagta gacgttgcaa taaaggtggc gaggcggcgt ggagaggagc
     3721 ggaaccgtgg tcccgggccg gggagccccg agcccgtccc tccccacgcc ccgccgcgct
     3781 ccccccggac cctggtcgct gagcccgggc gccgctcggc tgcgctatac atagtgtaca
     3841 ggagacatcg agtgtatttt taatgtcccc atatttctgt aaactagaaa cgcaacggac
     3901 tcctcgccac ggccgcgctc tccccgctgc gggcgcccag gaaggcggag acccgggaag
     3961 ccagggttcc ctgcgctccc gagctgagag ccaagtgctt taaggccggc gctctccttt
     4021 ccctttcctg tccacggccc gggcttccct ctcttccctc cagttcttgg cgaacacagg
     4081 tgaggcgacg gccggcttcg gtggatgcga gcttcccagc ggccaggagg gggaggggac
     4141 acccctccag ggggagaaac cgaggcccgc gcccaccgag gaagccctgc ccggtgcctt
     4201 cgtggaggag caggcgtctc tcctctgttg gcttgccgcc tgctccccct gtcccgtggc
     4261 tcctcgccaa agactgaatt tgtggagctg gagggcacac cctccccact ttccttcctg
     4321 ggacaggtga ggggccaatg ccagtctagg ggccgactca caggaggcct cgcgcagcct
     4381 cttggtcccc actctgcaag tcctgcctgg ggacccagcc cccctggtgg ttctggggcg
     4441 gagctttgct gcctagcagc aagtccttag ttactgtctc cagataccag gacctggagt
     4501 agggaatgga gtcatatggg ttcagttgtt cctggcgctt ctctgccccc tgctccccct
     4561 ctccccctct cgtaggacac aaggactttg gctttcttaa ctcatccttg gcgcttccgc
     4621 tccaccacgc ccacctgtgg ggaggtgccc tcagccctag agaggcgttt ggctggttcc
     4681 cttcccccag ggcacgttac taagaggaca ggcactgcat gctcctttaa gcgccctctg
     4741 ggactgggta cagtgcctcc agccccaggg ccctggtctg cgcacctagt tagacatcat
     4801 tgcccactcc agggccaggg ccactagctg acctcaccac ctttttcctt gagcccaagg
     4861 cagagagagc tgcagctggt gccatctaga caggctcaag tgtggccagt ggcagggctc
     4921 gagggccact gccctgttgc ttggctcagg acctctctga gatttgatgg ggactggata
     4981 ttcttccagg tagtagccat caagtcggaa gtgttggacc caggacctga cattccttca
     5041 agtctgccct cccttgctgt ggttttgcct tttggggcaa gagaggggct gggcaaacgg
     5101 ggaggaggca gtatcaacac cgattaggga accaaagttg cactacctgg gcccagcctc
     5161 tggttggcaa gagcaaagtt tctgttgatg aaaacaaaca gcccacaaca acaccccccc
     5221 ccccgttttc tgtgctccat gtgcaatatt tgttatgaac cttgtgtcgt tcaagtcacc
     5281 tttataatca ctgtagctag atgttccatg tccatccagg tgactttact ctgagtgcaa
     5341 tatttcaata gcctggtagt gagaagagtg ttgcttttgt ttcagccgac ctatgtgcag
     5401 ggcaatgcaa tgcagtccaa aacccttgta aataggagag gttgcaagcc aaatcaagag
     5461 tatttatcgt tattactatt attattaggc ctgcctttaa ttttagtgtt tcggtatttc
     5521 gcatcctgcc tcggtattga tcgtgtgttc tctgtgccaa tatgcaaagg agaggatcag
     5581 ttctttcctt tactgttgaa tgctcccatt tactgcttta aggcttttac tgtgttcatt
     5641 ttttagatac ctgtctgcac acaatgcaat aaaaataatt ttattacacc ctt
//
