LOCUS       NM_019345               4477 bp    mRNA    linear   ROD 13-OCT-2024
DEFINITION  Rattus norvegicus solute carrier family 12 member 3 (Slc12a3),
            mRNA.
ACCESSION   NM_019345
VERSION     NM_019345.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Wang,P., Zhu,G., Wu,Q., Shen,L., Liu,D., Wang,Z., Wang,W., Ren,Z.,
            Jia,Y., Liu,M., Xue,Y., Ji,D., Hu,Y., Yu,Y. and Wang,X.
  TITLE     Renal CD81 interacts with sodium potassium 2 chloride cotransporter
            and sodium chloride cotransporter in rats with
            lipopolysaccharide-induced preeclampsia
  JOURNAL   FASEB J 37 (4), e22834 (2023)
   PUBMED   36961378
  REMARK    GeneRIF: Renal CD81 interacts with sodium potassium 2 chloride
            cotransporter and sodium chloride cotransporter in rats with
            lipopolysaccharide-induced preeclampsia.
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Argaiz,E.R., Chavez-Canales,M., Ostrosky-Frid,M.,
            Rodriguez-Gama,A., Vazquez,N., Gonzalez-Rodriguez,X.,
            Garcia-Valdes,J., Hadchouel,J., Ellison,D. and Gamba,G.
  TITLE     Kidney-specific WNK1 isoform (KS-WNK1) is a potent activator of
            WNK4 and NCC
  JOURNAL   Am J Physiol Renal Physiol 315 (3), F734-F745 (2018)
   PUBMED   29846116
  REMARK    GeneRIF: Kidney-specific WNK1 isoform is a potent activator of WNK4
            and NCC-mediated sodium/chloride transport.
REFERENCE   3  (bases 1 to 4477)
  AUTHORS   Zachar,R., Al-Mashhadi,A., Dimke,H., Svenningsen,P., Jensen,B.L.
            and Carlstrom,M.
  TITLE     Hydronephrosis is associated with elevated plasmin in urine in
            pediatric patients and rats and changes in NCC and gamma-ENaC
            abundance in rat kidney
  JOURNAL   Am J Physiol Renal Physiol 315 (3), F547-F557 (2018)
   PUBMED   29767556
  REMARK    GeneRIF: The presence of active plasmin in urine in hydronephrosis
            could cause abnormal proteolytic activation of ENaC-gamma and
            impaired ability to excrete Na via NCC.
REFERENCE   4  (bases 1 to 4477)
  AUTHORS   Frindt,G., Yang,L., Uchida,S., Weinstein,A.M. and Palmer,L.G.
  TITLE     Responses of distal nephron Na+ transporters to acute volume
            depletion and hyperkalemia
  JOURNAL   Am J Physiol Renal Physiol 313 (1), F62-F73 (2017)
   PUBMED   28356292
  REMARK    GeneRIF: Kidneys respond acutely to hyperkalemic challenges by
            decreasing the activity of NCC while increasing that of ENaC.
REFERENCE   5  (bases 1 to 4477)
  AUTHORS   Blankenstein,K.I., Borschewski,A., Labes,R., Paliege,A., Boldt,C.,
            McCormick,J.A., Ellison,D.H., Bader,M., Bachmann,S. and Mutig,K.
  TITLE     Calcineurin inhibitor cyclosporine A activates renal Na-K-Cl
            cotransporters via local and systemic mechanisms
  JOURNAL   Am J Physiol Renal Physiol 312 (3), F489-F501 (2017)
   PUBMED   28003191
  REMARK    GeneRIF: Direct epithelial action of calcineurin inhibitor
            cyclosporine A is sufficient for the activation of renal NCC.
REFERENCE   6  (bases 1 to 4477)
  AUTHORS   Taniyama,Y., Sato,K., Sugawara,A., Uruno,A., Ikeda,Y., Kudo,M.,
            Ito,S. and Takeuchi,K.
  TITLE     Renal tubule-specific transcription and chromosomal localization of
            rat thiazide-sensitive Na-Cl cotransporter gene
  JOURNAL   J Biol Chem 276 (28), 26260-26268 (2001)
   PUBMED   11313351
REFERENCE   7  (bases 1 to 4477)
  AUTHORS   Kobayashi,K., Uchida,S., Mizutani,S., Sasaki,S. and Marumo,F.
  TITLE     Intrarenal and cellular localization of CLC-K2 protein in the mouse
            kidney
  JOURNAL   J Am Soc Nephrol 12 (7), 1327-1334 (2001)
   PUBMED   11423561
REFERENCE   8  (bases 1 to 4477)
  AUTHORS   Monroy,A., Plata,C., Hebert,S.C. and Gamba,G.
  TITLE     Characterization of the thiazide-sensitive Na(+)-Cl(-)
            cotransporter: a new model for ions and diuretics interaction
  JOURNAL   Am J Physiol Renal Physiol 279 (1), F161-F169 (2000)
   PUBMED   10894798
REFERENCE   9  (bases 1 to 4477)
  AUTHORS   Kunchaparty,S., Palcso,M., Berkman,J., Velazquez,H., Desir,G.V.,
            Bernstein,P., Reilly,R.F. and Ellison,D.H.
  TITLE     Defective processing and expression of thiazide-sensitive Na-Cl
            cotransporter as a cause of Gitelman's syndrome
  JOURNAL   Am J Physiol 277 (4), F643-F649 (1999)
   PUBMED   10516289
REFERENCE   10 (bases 1 to 4477)
  AUTHORS   Gamba,G., Miyanoshita,A., Lombardi,M., Lytton,J., Lee,W.S.,
            Hediger,M.A. and Hebert,S.C.
  TITLE     Molecular cloning, primary structure, and characterization of two
            members of the mammalian electroneutral sodium-(potassium)-chloride
            cotransporter family expressed in kidney
  JOURNAL   J Biol Chem 269 (26), 17713-17722 (1994)
   PUBMED   8021284
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Apr 13, 2021 this sequence version replaced NM_019345.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.35588.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-305               JAXUCZ010000019.1  10674746-10675050   c
            306-452             JAXUCZ010000019.1  10673612-10673758   c
            453-528             JAXUCZ010000019.1  10672386-10672461   c
            529-624             JAXUCZ010000019.1  10670729-10670824   c
            625-764             JAXUCZ010000019.1  10670379-10670518   c
            765-875             JAXUCZ010000019.1  10669938-10670048   c
            876-987             JAXUCZ010000019.1  10668931-10669042   c
            988-1118            JAXUCZ010000019.1  10668601-10668731   c
            1119-1203           JAXUCZ010000019.1  10663278-10663362   c
            1204-1358           JAXUCZ010000019.1  10662199-10662353   c
            1359-1466           JAXUCZ010000019.1  10661947-10662054   c
            1467-1590           JAXUCZ010000019.1  10661540-10661663   c
            1591-1692           JAXUCZ010000019.1  10659746-10659847   c
            1693-1848           JAXUCZ010000019.1  10658864-10659019   c
            1849-1948           JAXUCZ010000019.1  10658164-10658263   c
            1949-2060           JAXUCZ010000019.1  10657259-10657370   c
            2061-2201           JAXUCZ010000019.1  10656564-10656704   c
            2202-2308           JAXUCZ010000019.1  10654751-10654857   c
            2309-2391           JAXUCZ010000019.1  10653502-10653584   c
            2392-2493           JAXUCZ010000019.1  10651650-10651751   c
            2494-2605           JAXUCZ010000019.1  10650638-10650749   c
            2606-2692           JAXUCZ010000019.1  10647283-10647369   c
            2693-2828           JAXUCZ010000019.1  10646552-10646687   c
            2829-2896           JAXUCZ010000019.1  10645265-10645332   c
            2897-4477           JAXUCZ010000019.1  10636596-10638176   c
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19p13-p12"
     gene            1..4477
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /note="solute carrier family 12 member 3"
                     /db_xref="GeneID:54300"
                     /db_xref="RGD:3686"
     exon            1..305
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..3038
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /note="thiazide-sensitive sodium-chloride cotransporter;
                     Na-Cl symporter; thiazide-sensitive Na-Cl cotransporter;
                     solute carrier family 12 (sodium/chloride transporters),
                     member 3; solute carrier family 12 (sodium/chloride
                     transporter), member 3"
                     /codon_start=1
                     /product="solute carrier family 12 member 3"
                     /protein_id="NP_062218.3"
                     /db_xref="GeneID:54300"
                     /db_xref="RGD:3686"
                     /translation="MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLT
                     HGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALA
                     FDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWI
                     TAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIG
                     LIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAG
                     MEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGI
                     DGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIG
                     SCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSA
                     FAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYL
                     LAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKW
                     AALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALS
                     YSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQ
                     RVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGF
                     KKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTAPEALVQEEQ
                     TSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEE
                     RKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEM
                     RRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYM
                     AWLETLSQDLSPPVLLIRGNQENVLTFYCQ"
     exon            306..452
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            453..528
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            529..624
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            625..764
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            765..875
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            876..987
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1118
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1203
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1358
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1466
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1590
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1692
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1848
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1948
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2060
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2201
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2308
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2391
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2493
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2605
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2692
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2828
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..2896
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..4477
                     /gene="Slc12a3"
                     /gene_synonym="NCC; TSC"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgtcccctcc tttgacaccc ggagccataa tggcagagct acccgtgaca gagatgccag
       61 gcgatgctct gtgcagtggg cgcttcacca tcagcacgct gctggggggt gacgagcccc
      121 caccagctgc ctgtgacaat agccagccca gccatttgac ccatggcagc accttgtacc
      181 tacgcacctt tggttacaac actatagatg tggtacccgc ctacgaacac tacgctaaca
      241 gcgctctgcc cggcgagccc cggaaggtgc ggcccaccct ggctgacctg cactcgttcc
      301 tcaagcagga aggtagccac ctgcatgccc tggcctttga tggacggcca ggccatgagc
      361 tgactgatgg acttgtggaa gacgagacag gcgccaacag cgagaagagc cctggagaac
      421 ctgtgcgttt cggctgggtc aagggagtga tgatccgatg catgctcaac atatggggtg
      481 tgatcctcta tctgcggctg ccctggatta cggcgcaggc gggcattgtc ctgacctggc
      541 tcatcatcct gctgtcagtc atggtgacat ctatcaccgg cctctctatc tcggccatct
      601 ccaccaatgg caaggtcaag tcgggtggta cctatttcct aatttctcgg agtctgggac
      661 ctgagctggg gggctccatt ggcctcatct tcgcctttgc caatgccgtg ggtgtggcca
      721 tgcacactgt gggctttgca gagaccgtac gagacctact tcaggagaac ggcacaccca
      781 ttgtagaccc catcaatgac atccgcatca tcggtgtggt cacggtcact gtgctgctgg
      841 ccatctcgct ggctgggatg gagtgggagt ccaaggccca ggtgctgttc tttcttgtca
      901 tcatggtctc ttttgccaac tacctggtgg gcacgctgat cccagcatct aaggacaaag
      961 cctccaaagg tttctacagc taccatgggg acatttttgt tcagaacctg gtacccgact
     1021 ggagaggcat cgatggtagc ttcttcggga tgttctccat cttcttccct tcagccacag
     1081 gcattctggc aggggccaac atctctgggg acctcaagga ccctgctgtc gctattccca
     1141 agggaacact catggccatt ttctggacca ccatttccta ccttgccatc tcggccacca
     1201 tcgggtcctg tgtggtgagg gatgcctctg gggacgtgaa cgacaccgta actcctggcc
     1261 caggcctctg tgaagggcta gcctgcggtt atggctggaa cttcacggaa tgctcccagc
     1321 agcacagctg ccgctatggc ctcatcaact actaccagac catgagcatg gtgtctgcct
     1381 tcgcacccct gatcacagcc ggcatctttg gggccaccct gtcctcggcc ttggcctgtc
     1441 ttgtctcagc tgccaaggtc ttccagtgcc tgtgcgaaga ccagctgtac cctctgattg
     1501 gcttctttgg caagggctat gggaagaaca aggaacctgt gcgtggctac ttgctggcct
     1561 acgccatcgc ggtggccttc atcatcattg ctgagctcaa caccatcgct cccatcattt
     1621 ctaacttctt cctctgctcc tacgccctca tcaacttcag ctgcttccat gcctccatca
     1681 ccaactcacc tggatggaga ccctccttcc gatactacag caagtgggcg gcgctgtttg
     1741 gggcagtgat ctccgtggtc atcatgttcc tgcttacctg gtgggcagct ctcattgcca
     1801 ttggagtcgt tctcttcctc ctgctctatg tgatctacaa gaagccagag gtaaactggg
     1861 gctcctcagt gcaggctggc tcctacaacc tggccctgag ctactccgtg ggcctcaatg
     1921 aggtggaaga ccacatcaag aactatcgcc cccagtgcct ggtgcttaca ggacccccca
     1981 acttccgccc agctcttgtg gactttgtga gcacgtttac ccagaaccta agcctgatga
     2041 tctgtggcca cgtgctcatt gcacccggca aacagagagt gccggagctc cgactcatcg
     2101 ccagcggaca caccaagtgg ctgaacaaga ggaagatcaa ggccttctac tctgacgtca
     2161 tagctgagga cttgcgcagt ggcgtccaga ttctcatgca ggcctcgggt ctgggaagaa
     2221 tgaaacctaa cattctggtg gttggcttca agaagaactg gcagtctgcc cacccagcca
     2281 cactggagga ctacattggc atcctgcacg atgcctttga cttcaactac ggggtgtgca
     2341 tcatgaggat gcgtgagggg ctcaatgtct ctgaggcgct gcagacgcac acagccccag
     2401 aggcactggt acaggaggag cagaccagca ccatcttcca gtcggagcaa ggcaagaaga
     2461 ccattgacat ctactggctg tttgacgatg gaggcctcac cctcctcatc ccttatctcc
     2521 tgcaccgcaa gaagagatgg ggcaaatgca agatccgcgt gttcgttggg ggccagatca
     2581 acaggatgga tgaagagaga aaggcgatca tttctctgtt gagcaagttc agactgggat
     2641 tccatgaagt ccacgtcctt cctgacatca accagaagcc ccaggtggag cacaccaagc
     2701 ggtttgagga catgattgcc cccttccgtc tcaatgacgg cttcaaggac gaggccactg
     2761 ttgccgagat gaggagggac tatccctgga agatctcaga cgaggagatt aataagaaca
     2821 gaattaagtc ccttcggcag gtgaggctga atgagattct cctggattac tcccgggatg
     2881 ctgctctgat catccttacc ttgcccattg gaaggaaggg gaagtgcccc agttcactgt
     2941 acatggcctg gctggagacc ctgtcccagg acctcagtcc tcccgttctc ctcatccgag
     3001 gtaaccagga gaacgtgctc accttttact gccagtgatg tccagtgtgg cagttcctgc
     3061 cgagagctga gcgcgtggga tagagtcgga actcacgctc tccagctgga gaagaggctt
     3121 caattcctgc tgtgtcatca catctaccgg ctgtgtttcc aagctggctg caccccatca
     3181 gacacacctt ctagaccttg aagttgcacg gaaaagcttc tggaagagtc ttatctcttt
     3241 ttaaaaagct tttctttttt ttttttgtct tgatgaccga aatattagtc attgattcag
     3301 tctttgattt gtatacaaat cgagtcccag tattgggtgt gcaacttgac aatctctctc
     3361 gggaccttcc ctgtgaccac ccccaaagct gtctcatctc ccccagacag agcaggagcc
     3421 catcctgagg acccatgtgg agttctgatg accacaggac cctttgagct acacagcaac
     3481 acacatttct cctcctgttc cctttcagtg atggatcctg ggtgctccta ctagttattt
     3541 agaccagggt cctctgggtt ctttgattac aaattctatg gtttcctcag agactcagaa
     3601 cacacagaac aatggtaggg aagcatcaca aactggtcct cactctcgag ggtgcttctc
     3661 tcccctctcc cctctcccct ctcccctctc ccctctcccc tctcccctct cccctctccc
     3721 ccctctctcc cctctctccc ctctcccctc tcccctctcc cctctctccc ctctctcccc
     3781 tcccccccct ccctctctct ctcctttttc cttctgacag agtcttatgt ggtccaggct
     3841 ggccttgaac ttgttctgta gtcaagaata gcttcaaaca tctgatcccc ctgcctccat
     3901 tacagagcac agggattcca ggcacattca cataggcatg tttcacgagg tgatagggac
     3961 cacacctacg gttgatgggt gccggcaatc acctgctgac tatgctaccc ccgcccagtc
     4021 tctagtgtgc tcctttgaag ttgattgcaa ccttctaaat gatcttcaaa cccactcgtg
     4081 tgcagtgacc cgtgctttga aaaacaggtt taatttctgg aggttgtgtg tagggtctgt
     4141 tgctggttgg tttctttgtt gctcgggaat tttgttttgt caacttgaca ggagctaggg
     4201 ccatctaagc agaaggaacc tcaattgaca aaatgcctcc tgctgattgg cggcctgtaa
     4261 tgattgacgg ggtgggggcc agcccactgt gttgccgtac caccatgagc aggtggtcct
     4321 ggggtgtata agaaagcagg ctgagcaagc tatgggagca agccagtgag gagtgtctct
     4381 tcatggcttc tgcttcagtt cctgcctgga gttccttccc tgacttctct tcattataaa
     4441 ccataagcca taagatgaaa taaacccttt tctcccc
//
