LOCUS       NM_001079705            3614 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus shootin 1 (Shtn1), transcript variant 1, mRNA.
ACCESSION   NM_001079705 XM_001073607 XM_006231671 XM_214734
VERSION     NM_001079705.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Higashiguchi,Y., Katsuta,K., Minegishi,T., Yonemura,S., Urasaki,A.
            and Inagaki,N.
  TITLE     Identification of a shootin1 isoform expressed in peripheral
            tissues
  JOURNAL   Cell Tissue Res 366 (1), 75-87 (2016)
   PUBMED   27177867
  REMARK    GeneRIF: These results suggest that shootin1b may function in
            various peripheral tissues including epithelial cells.
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Kubo,Y., Baba,K., Toriyama,M., Minegishi,T., Sugiura,T., Kozawa,S.,
            Ikeda,K. and Inagaki,N.
  TITLE     Shootin1-cortactin interaction mediates signal-force transduction
            for axon outgrowth
  JOURNAL   J Cell Biol 210 (4), 663-676 (2015)
   PUBMED   26261183
  REMARK    GeneRIF: The shootin1-cortactin interaction participates in
            netrin-1-induced F-actin adhesion coupling and in the promotion of
            traction forces for axon outgrowth.
REFERENCE   3  (bases 1 to 3614)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3614)
  AUTHORS   Sapir,T., Levy,T., Sakakibara,A., Rabinkov,A., Miyata,T. and
            Reiner,O.
  TITLE     Shootin1 acts in concert with KIF20B to promote polarization of
            migrating neurons
  JOURNAL   J Neurosci 33 (29), 11932-11948 (2013)
   PUBMED   23864681
REFERENCE   5  (bases 1 to 3614)
  AUTHORS   Toriyama,M., Kozawa,S., Sakumura,Y. and Inagaki,N.
  TITLE     Conversion of a signal into forces for axon outgrowth through
            Pak1-mediated shootin1 phosphorylation
  JOURNAL   Curr Biol 23 (6), 529-534 (2013)
   PUBMED   23453953
  REMARK    GeneRIF: Shootin1 is located at a critical interface, transducing a
            chemical signal into traction forces for axon outgrowth. A
            chemoattractant, netrin-1, positively regulates traction forces at
            axonal growth cones via Pak1-mediated shootin1 phosphorylation.
REFERENCE   6  (bases 1 to 3614)
  AUTHORS   Pericacho,M., Velasco,S., Prieto,M., Llano,E., Lopez-Novoa,J.M. and
            Rodriguez-Barbero,A.
  TITLE     Endoglin haploinsufficiency promotes fibroblast accumulation during
            wound healing through Akt activation
  JOURNAL   PLoS One 8 (1), e54687 (2013)
   PUBMED   23349951
REFERENCE   7  (bases 1 to 3614)
  AUTHORS   Shimada,T., Toriyama,M., Uemura,K., Kamiguchi,H., Sugiura,T.,
            Watanabe,N. and Inagaki,N.
  TITLE     Shootin1 interacts with actin retrograde flow and L1-CAM to promote
            axon outgrowth
  JOURNAL   J Cell Biol 181 (5), 817-829 (2008)
   PUBMED   18519736
  REMARK    GeneRIF: Shootin1 interacts with actin retrograde flow and L1-CAM
            to promote axon outgrowth
REFERENCE   8  (bases 1 to 3614)
  AUTHORS   Mori,T., Wada,T., Suzuki,T., Kubota,Y. and Inagaki,N.
  TITLE     Singar1, a novel RUN domain-containing protein, suppresses
            formation of surplus axons for neuronal polarity
  JOURNAL   J Biol Chem 282 (27), 19884-19893 (2007)
   PUBMED   17439943
REFERENCE   9  (bases 1 to 3614)
  AUTHORS   Toriyama,M., Shimada,T., Kim,K.B., Mitsuba,M., Nomura,E.,
            Katsuta,K., Sakumura,Y., Roepstorff,P. and Inagaki,N.
  TITLE     Shootin1: A protein involved in the organization of an asymmetric
            signal for neuronal polarization
  JOURNAL   J Cell Biol 175 (1), 147-157 (2006)
   PUBMED   17030985
  REMARK    GeneRIF: Shootin1 is involved in the generation of internal
            asymmetric signals required for neuronal polarization.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1, BC085922.1 and CO392889.1.
            
            On Jan 13, 2015 this sequence version replaced NM_001079705.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1, also known as shootin
            1b).
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF055488.1, SRR26360190.292697.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760393,
                                           SAMEA5760476 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                JAXUCZ010000001.1  268211915-268211947 c
            34-734              BC085922.1         1-701
            735-829             CO392889.1         320-414
            830-3614            BC085922.1         702-3486
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q55"
     gene            1..3614
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /note="shootin 1"
                     /db_xref="GeneID:292139"
                     /db_xref="RGD:1311558"
     exon            1..176
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..2020
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="shootin-1 isoform 1"
                     /protein_id="NP_001073173.2"
                     /db_xref="GeneID:292139"
                     /db_xref="RGD:1311558"
                     /translation="MNSSDEEKQLQLITSLKEQAIGEYEDLRAENQKTKETCDKIRQE
                     RDEAVKKLEEFQKISHMVIEEVNFMQNHLEIEKTCRESAEALATKLNKENKTLKRISM
                     LYMAKLGPDVITEEINIDDDDPGTDTDAAAETCVSVQCQKQIKELRDQIVSVQEEKKV
                     LAIELESLKSKLGEVMEEVNKVKQEKAVLNSEVLEQRKVLEKCNRVSVLAVEEYEELQ
                     VNLELEKDLRKKAESFAQEMFIEQNKLKRQSHLLLQSSLPDQQLLKALDENAKLIQQL
                     EEERIQHQQKVKELEERLENEALHKEIHNLRQQLELLEDDKRELEQKYQSSEEKARNL
                     KHSVDELQKRVNQSENSVPPPPPPPPPLPPPPPNPIRSLMSMIRKRSHPSGGSTKKEK
                     ATQPETAEEVTDLKRQAVEEMMDRIKKGVHLRPVNQTARPKAKPDSLKGSESAVDELK
                     GILGTLNKSTSSRSLKSLGPENSETELERILRRRKLTAEADSSSPTGILATSESKSMP
                     VLGSVSSVTKSALNKKTLEAEFNNPCPLTPEPGEGPRKLEGCTNSKVTFQPPSKGGYR
                     RKCVGSENQSEPVVVLDPVSTHEPQTKDQAAEKDPTQCKEEERGETQPEFKEDSSGGK
                     TGETDSSNC"
     exon            177..229
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            230..290
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            291..385
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            386..554
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            555..652
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            653..734
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            735..829
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            830..976
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1130
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1230
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1313
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1423
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1477
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1598
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1791
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..3495
                     /gene="Shtn1"
                     /gene_synonym="RGD1311558; Shootin1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacttccggg tagtgcagcc gcacgccgcg ccaccggcat cttgctttct gctccccctc
       61 ccccgtgtcc ccctcgcctc tccctccttg ccttttatcc ccagccccac ccgccaaaat
      121 gaacagctcg gacgaggaga agcagctgca gctcatcacc agcttgaagg aacaagccat
      181 aggcgaatat gaagaccttc gagcagagaa ccagaaaaca aaggagacgt gtgacaagat
      241 taggcaagaa cgagatgaag ctgttaagaa actggaagag tttcagaaaa tttctcatat
      301 ggttatagag gaagtaaatt tcatgcagaa ccaccttgag atagagaaga catgccgaga
      361 gagtgctgag gccttggcga caaagctaaa taaagaaaat aaaaccctga aaagaatcag
      421 catgctatac atggccaaac tggggccaga cgtaataaca gaggagataa acattgatga
      481 tgacgaccca ggcaccgaca cagacgccgc ggcagagacc tgtgtgtccg tccagtgtca
      541 gaagcaaatc aaagaacttc gagatcaaat tgtgtctgtt caggaagaaa agaaagtgtt
      601 agccattgag ctggaaagcc tcaagagcaa actcggggag gtgatggagg aggtaaataa
      661 agttaagcaa gaaaaagctg tcttgaattc agaagttctt gagcagagga aagtcttaga
      721 aaaatgcaac agagtgtccg tgttagccgt cgaggagtat gaggagctac aggtgaacct
      781 ggagctggag aaggaccttc gcaagaaggc agagtctttt gcacaagaga tgttcattga
      841 acaaaacaag ctgaagagac aaagccacct tctgctgcag agctcccttc ctgaccagca
      901 gcttctgaaa gctttagatg aaaatgcaaa acttatccag cagcttgaag aagagaggat
      961 ccagcatcag caaaaggtca aagagttaga ggaacggcta gagaatgaag cgcttcacaa
     1021 agagatccac aacctcagac aacagctgga gcttctggag gatgacaaga gagagctgga
     1081 gcagaaatac cagagctcgg aggaaaaggc ccggaacctg aagcattcag tggacgaact
     1141 ccagaagcga gtgaatcagt cggagaattc ggtgcctccc ccgcctcctc ctcctccacc
     1201 tcttccacct ccgcctccca atccaatccg gtccctcatg tcaatgatcc ggaagcgatc
     1261 ccaccccagt ggcggtagca ctaagaagga aaaggcaact cagccagaga cagctgagga
     1321 agtcacagac ctgaagaggc aagcagtgga agagatgatg gacagaatta aaaagggagt
     1381 tcatcttaga ccagttaacc agacagccag acccaaggca aagccagact ctctaaaggg
     1441 ctcagaaagt gcagtggatg aactgaaggg aatcctgggg acacttaaca aatccactag
     1501 ttcgagaagc ttaaaatccc ttggccctga gaacagtgaa actgagttag agaggatttt
     1561 acgccgcaga aagctgacag cagaagcaga tagcagtagt cctaccggga tattagccac
     1621 ctcagagtcc aagtccatgc cagtgttggg ttctgtatcc agtgtaacaa aatcagcctt
     1681 gaacaagaaa actctggagg cagaattcaa caatccgtgt cccctaacac ctgagccagg
     1741 tgaagggccc cgtaaattgg aaggatgcac gaattccaag gttacctttc agcctcccag
     1801 taaaggtgga tacaggagaa aatgtgtggg cagtgaaaac cagtctgagc cggttgtagt
     1861 tttagatcct gtttctacac atgaacccca aaccaaagac caggctgctg agaaagaccc
     1921 aacccaatgc aaggaggagg agagaggcga aacacaacca gaattcaaag aagacagcag
     1981 tggtggaaaa acgggagaga cggacagttc taactgctga tctgaaacca gaggctggcg
     2041 tgtttgcggg gccttctcag tgagcacgtg gttcacctgt gtgtactgtc aagaactgtt
     2101 ggacattcct ttgttcccca cactcctttg ctggtatcac tttgtaagta gcaattatga
     2161 acataagtaa gctgtttagc aaaacgcatc ttctgggtag tttttaatct ttacggggta
     2221 agggttcttt ctagtttcta tatgaagtat gactttcttg agactgtggc agaactcaga
     2281 tgttggtgtc tttacaggga acgtgcttac cactcatcaa attattcatg ggaaaattaa
     2341 aggtggttgc tggtaactgg ataaacagag accaccaaac ttaacaatta tcttaagggg
     2401 caccctctaa tccaagccca gcacatgggg agactaaggc aagaggatca cttgagcctg
     2461 agcttgagtt cgggtcacat agcaagactc catataaaga aaaggcctgg aagagtctct
     2521 tgtttgagtc tggtcttctg atgggaaact gcagcagtca ggacattagt gggtccttta
     2581 gagtctccat tttgtagggt acacgagacc ggccagctct gagctcagga ggtggcatca
     2641 tggtctctta ggtctgagtt gagcacagtt gactattgtg tggaaaacgt gaccctgtgt
     2701 cccaggctta gagagtcctt caggggtgat atcctggcag cctcaacaga gacttggaca
     2761 gatgctcaaa ccttgaattt aacagggatg tctcagtaaa ctacccattc tgttccatga
     2821 gttaactgtg ttttcccaca cattcacagg gcacaagtac catatgtaac gcccgttctt
     2881 atgcacaggg aagataagta acaagcacac attgtttttc ttccttcact gaggatctat
     2941 actacatggg tcttttcttg ttttgttttt ttgtttgttt gtttttaggg gaaattaagt
     3001 ggtagtttct aaaaatacaa tgtttcagac taccacggtg actaaataga aatgtaatca
     3061 gggatttaag aaaaaaaact tatgcaggtt ttcaaagttg attgtttcaa aattggtgtt
     3121 tatttaaaat aagtggtaat gtacttgagt gcacttttta tgataatgat tcagtaatgg
     3181 taattttact attaaggaaa tcgaaaggct tagttttgtt agcatgactc ggcatgtagc
     3241 tgtcaggtgc tccccacctg agggcaaaag aaaatgatag taacaattgc agtagttgta
     3301 tcctattgta tttttgcacg tgtgctacta gtgggcctgg ggaggtggga aggcaggtga
     3361 ggatacttcc tcggcttggg gacaattctc tttctgtagg ttcactgggc ttgactattt
     3421 cccctgtctg tcagtgctga ttttatatcc ctgtcagttt actcatgggg aattaaaaca
     3481 tgattttttt ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3601 aaaaaaaaaa aaaa
//
