LOCUS       NM_001008322            2295 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus serine hydroxymethyltransferase 2 (Shmt2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001008322 XM_216909
VERSION     NM_001008322.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Zhou,Y., Kang,L., Xu,R., Zhao,D., Wang,J., Wu,J., Lin,H., Ding,Z.
            and Zou,Y.
  TITLE     Mitochondrial outer membrane protein Samm50 protects against
            hypoxia-induced cardiac injury by interacting with Shmt2
  JOURNAL   Cell Signal 120, 111219 (2024)
   PUBMED   38723737
  REMARK    GeneRIF: Mitochondrial outer membrane protein Samm50 protects
            against hypoxia-induced cardiac injury by interacting with Shmt2.
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Minton,D.R., Nam,M., McLaughlin,D.J., Shin,J., Bayraktar,E.C.,
            Alvarez,S.W., Sviderskiy,V.O., Papagiannakopoulos,T.,
            Sabatini,D.M., Birsoy,K. and Possemato,R.
  TITLE     Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial
            Translation Initiation and Maintenance of Formylmethionyl-tRNAs
  JOURNAL   Mol Cell 69 (4), 610-621 (2018)
   PUBMED   29452640
REFERENCE   3  (bases 1 to 2295)
  AUTHORS   Morscher,R.J., Ducker,G.S., Li,S.H., Mayer,J.A., Gitai,Z., Sperl,W.
            and Rabinowitz,J.D.
  TITLE     Mitochondrial translation requires folate-dependent tRNA
            methylation
  JOURNAL   Nature 554 (7690), 128-132 (2018)
   PUBMED   29364879
REFERENCE   4  (bases 1 to 2295)
  AUTHORS   Yang,X., Wang,Z., Li,X., Liu,B., Liu,M., Liu,L., Chen,S., Ren,M.,
            Wang,Y., Yu,M., Wang,B., Zou,J., Zhu,W.G., Yin,Y., Gu,W. and Luo,J.
  TITLE     SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation
  JOURNAL   Cancer Res 78 (2), 372-386 (2018)
   PUBMED   29180469
REFERENCE   5  (bases 1 to 2295)
  AUTHORS   Tani,H., Ohnishi,S., Shitara,H., Mito,T., Yamaguchi,M.,
            Yonekawa,H., Hashizume,O., Ishikawa,K., Nakada,K. and Hayashi,J.I.
  TITLE     Mice deficient in the Shmt2 gene have mitochondrial respiration
            defects and are embryonic lethal
  JOURNAL   Sci Rep 8 (1), 425 (2018)
   PUBMED   29323231
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2295)
  AUTHORS   Fu,T.F., Rife,J.P. and Schirch,V.
  TITLE     The role of serine hydroxymethyltransferase isozymes in one-carbon
            metabolism in MCF-7 cells as determined by (13)C NMR
  JOURNAL   Arch Biochem Biophys 393 (1), 42-50 (2001)
   PUBMED   11516159
REFERENCE   7  (bases 1 to 2295)
  AUTHORS   Verleysdonk,S., Martin,H., Willker,W., Leibfritz,D. and
            Hamprecht,B.
  TITLE     Rapid uptake and degradation of glycine by astroglial cells in
            culture: synthesis and release of serine and lactate
  JOURNAL   Glia 27 (3), 239-248 (1999)
   PUBMED   10457370
REFERENCE   8  (bases 1 to 2295)
  AUTHORS   Garrow,T.A., Brenner,A.A., Whitehead,V.M., Chen,X.N., Duncan,R.G.,
            Korenberg,J.R. and Shane,B.
  TITLE     Cloning of human cDNAs encoding mitochondrial and cytosolic serine
            hydroxymethyltransferases and chromosomal localization
  JOURNAL   J Biol Chem 268 (16), 11910-11916 (1993)
   PUBMED   8505317
REFERENCE   9  (bases 1 to 2295)
  AUTHORS   Masuda,T., Sakamoto,M., Nishizaki,I., Hayashi,H., Yamamoto,M. and
            Wada,H.
  TITLE     Affinity purification and characterization of serine
            hydroxymethyltransferases from rat liver
  JOURNAL   J Biochem 101 (3), 643-652 (1987)
   PUBMED   3110140
REFERENCE   10 (bases 1 to 2295)
  AUTHORS   Motokawa,Y. and Kikuchi,G.
  TITLE     Glycine metabolism in rat liver mitochondria. V. Intramitochondrial
            localization of the reversible glycine cleavage system and serine
            hydroxymethyltransferase
  JOURNAL   Arch Biochem Biophys 146 (2), 461-464 (1971)
   PUBMED   4107181
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Mar 18, 2021 this sequence version replaced NM_001008322.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085331.1, SRR26360172.3726.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760386,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000007.1  65249423-65249567   c
            146-343             JAXUCZ010000007.1  65248013-65248210   c
            344-423             JAXUCZ010000007.1  65247437-65247516   c
            424-624             JAXUCZ010000007.1  65247071-65247271   c
            625-706             JAXUCZ010000007.1  65246769-65246850   c
            707-829             JAXUCZ010000007.1  65246549-65246671   c
            830-969             JAXUCZ010000007.1  65246274-65246413   c
            970-1135            JAXUCZ010000007.1  65245909-65246074   c
            1136-1235           JAXUCZ010000007.1  65245605-65245704   c
            1236-1391           JAXUCZ010000007.1  65245365-65245520   c
            1392-1499           JAXUCZ010000007.1  65245165-65245272   c
            1500-2295           JAXUCZ010000007.1  65244247-65245042   c
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..2295
                     /gene="Shmt2"
                     /note="serine hydroxymethyltransferase 2"
                     /db_xref="GeneID:299857"
                     /db_xref="RGD:1308582"
     exon            1..145
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1627
                     /gene="Shmt2"
                     /EC_number="2.1.2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine hydroxymethyltransferase, mitochondrial; serine
                     hydroxymethyltransferase 2 (mitochondrial)"
                     /codon_start=1
                     /product="serine hydroxymethyltransferase, mitochondrial
                     isoform 1"
                     /protein_id="NP_001008323.1"
                     /db_xref="GeneID:299857"
                     /db_xref="RGD:1308582"
                     /translation="MVPFSLLRTTRPLQRCGQLVCMAARAQHSEVAQKQAGEAAGGWT
                     GQESLSDSDPEIWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPG
                     KRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAAYTALLQPHDR
                     IMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLALTARLFRP
                     RLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKYADIVTT
                     TTHKTLRGARSGLIFYRKGVRTVDPKTGQEIPYTFEDRINFAVFPSLQGGPHNHAIAA
                     VAVALKQACTPMFREYSLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLD
                     GARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDE
                     GVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVEQFARGFPMPGFDER"
     exon            146..343
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            344..423
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            424..624
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            625..706
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            707..829
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            830..969
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1135
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1235
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1391
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1499
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..2295
                     /gene="Shmt2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggacttcacg ggggcatttt ctcgcgcatg cgctctagaa gcagccggtt tccgaccccg
       61 gtactacacc aatacaagtt agcggctggt cctcccgtgg cctccggtgg cgatggtacc
      121 cttctcttta cttcggacca ctcggcctct gcagagatgt ggtcagctgg tctgcatggc
      181 tgcccgggcc cagcacagtg aggtggccca gaagcaggct ggggaagcag ctggaggctg
      241 gacgggccag gagagtttat cagacagtga ccctgagata tgggagcttc tgcagaggga
      301 gaaggacaga cagtgtcgtg gcctggagct catcgcctca gagaacttct gcagccgagc
      361 tgcgctggag gccctggggt cctgtctcaa caacaagtac tcggagggtt accctggcaa
      421 gagatactac ggaggagcag aagtcgtgga cgagattgag ctgctgtgtc agcgccgggc
      481 cttggaagcc tttgacctgg atcctgcaca gtggggagtc aatgtgcagc cgtattcagg
      541 gtccccagcc aacctggctg cctacaccgc ccttctgcag cctcatgatc gaatcatggg
      601 gttggacctg cccgatgggg gccacctcac ccacggctac atgtctgatg tcaagcggat
      661 ctctgccacc tccatcttct tcgagtccat gccgtataag ctcaatcccc aaaccggcct
      721 catcgactac gaccagctag cgctgactgc tcggcttttc cgacctcggc tcatcatagc
      781 tggcactagt gcctatgccc gcctcattga ctatgcacga atgagagagg tgtgtgacga
      841 agtcaaggca cacctgctgg cagacatggc ccatatcagt ggcctggtgg ctgccaaggt
      901 gatcccctca cctttcaagt atgcggacat cgtcaccacc accactcaca agactcttcg
      961 aggggccagg tcaggactca tcttttaccg gaagggagtg cggactgtag accccaagac
     1021 tggccaagag atcccttata cttttgagga cagaatcaac ttcgctgtgt tcccatcctt
     1081 acaagggggc ccccacaatc acgccattgc tgcagtagct gtggctctca agcaggcctg
     1141 cacccctatg ttccgggagt actccttaca agtgttgcgg aacgcccaag ccatggccga
     1201 cgccctgctc aagcgaggat actcgctggt gtctggtggc actgacaccc acctggtgct
     1261 ggtggacctg cggcccaagg gcctggatgg agcacgggcc gagcgtgtgt tggagcttgt
     1321 gtccatcaca gccaacaaga acacctgtcc tggagaccgg agtgccatta ctccgggagg
     1381 cttgaggctt ggggccccag cattgacttc tcgtcaattc cgtgaggatg acttccggag
     1441 ggtcgttgat tttatcgatg aaggagtcaa cattggcttg gaggtgaagc gcaagactgc
     1501 caaacttcag gatttcaagt ccttcctgct caaggaccca gaaacaagtc agcgtttggc
     1561 caacctccgg caacaggtgg aacagtttgc caggggcttc ccaatgcctg gatttgatga
     1621 acgttgagga cgcccagcag tcaaagcccg cagaccggaa gttagctttc tttctgcctc
     1681 cctggactag gaaagagatg ccatctcttt gggttttagt ggagggagga aggccttcca
     1741 tttagggcag gagccaggca taaagtcccc tccagaatct gtagctgaga tttctgattt
     1801 taatcatgtc tacaagaagc actcaataca catccagctt gttttttgag atggggtctc
     1861 agttagctga gactggcctt gaaatgaccc tcctacctcc atttcccaag tgctggggtt
     1921 ataggtatgc gctgtgtgac acccaacttc accctccact tcccaccctg gccttccacc
     1981 acatctcagt gttacagact cctgctctct cctagggaag gggagttgca agctgagcca
     2041 gaggaagggg tgggtaaagc gctctccttc ctgtttttat ctaataaaat gctaacctgc
     2101 ccagagttcc tattactgtg ggaggggtcc cctgggccag ccagtgattc cactccctca
     2161 acatgtattt ctccttccct cccaccacca caaggacccc cggggctgta gcctcctctc
     2221 tgatcagagc cgacaccaga cgtgattagc aggcgcagca aattcaattt gttaaatgaa
     2281 attgtatttt gccca
//
