LOCUS       NM_001011986            1633 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus splicing factor 3A subunit 2 (Sf3a2), mRNA.
ACCESSION   NM_001011986 XM_216844
VERSION     NM_001011986.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Zhang,X., Yan,C., Zhan,X., Li,L., Lei,J. and Shi,Y.
  TITLE     Structure of the human activated spliceosome in three
            conformational states
  JOURNAL   Cell Res 28 (3), 307-322 (2018)
   PUBMED   29360106
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 1633)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 1633)
  AUTHORS   Chen,Y.I., Moore,R.E., Ge,H.Y., Young,M.K., Lee,T.D. and
            Stevens,S.W.
  TITLE     Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes
            expands the catalog of participating factors
  JOURNAL   Nucleic Acids Res 35 (12), 3928-3944 (2007)
   PUBMED   17537823
REFERENCE   5  (bases 1 to 1633)
  AUTHORS   Tanackovic,G. and Kramer,A.
  TITLE     Human splicing factor SF3a, but not SF1, is essential for pre-mRNA
            splicing in vivo
  JOURNAL   Mol Biol Cell 16 (3), 1366-1377 (2005)
   PUBMED   15647371
REFERENCE   6  (bases 1 to 1633)
  AUTHORS   Gringel,S., van Bergeijk,J., Haastert,K., Grothe,C. and Claus,P.
  TITLE     Nuclear fibroblast growth factor-2 interacts specifically with
            splicing factor SF3a66
  JOURNAL   Biol Chem 385 (12), 1203-1208 (2004)
   PUBMED   15653435
REFERENCE   7  (bases 1 to 1633)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   8  (bases 1 to 1633)
  AUTHORS   Nesic,D. and Kramer,A.
  TITLE     Domains in human splicing factors SF3a60 and SF3a66 required for
            binding to SF3a120, assembly of the 17S U2 snRNP, and
            prespliceosome formation
  JOURNAL   Mol Cell Biol 21 (19), 6406-6417 (2001)
   PUBMED   11533230
REFERENCE   9  (bases 1 to 1633)
  AUTHORS   Neubauer,G., King,A., Rappsilber,J., Calvio,C., Watson,M., Ajuh,P.,
            Sleeman,J., Lamond,A. and Mann,M.
  TITLE     Mass spectrometry and EST-database searching allows
            characterization of the multi-protein spliceosome complex
  JOURNAL   Nat Genet 20 (1), 46-50 (1998)
   PUBMED   9731529
REFERENCE   10 (bases 1 to 1633)
  AUTHORS   Hong,W., Bennett,M., Xiao,Y., Feld Kramer,R., Wang,C. and Reed,R.
  TITLE     Association of U2 snRNP with the spliceosomal complex E
  JOURNAL   Nucleic Acids Res 25 (2), 354-361 (1997)
   PUBMED   9016565
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079320.1.
            
            On Feb 11, 2005 this sequence version replaced XM_216844.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079320.1, DN948235.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..1633
                     /gene="Sf3a2"
                     /note="splicing factor 3A subunit 2"
                     /db_xref="GeneID:299620"
                     /db_xref="RGD:1308627"
     exon            1..45
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            46..207
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1497
                     /gene="Sf3a2"
                     /note="splicing factor 3a, subunit 2, 66kDa"
                     /codon_start=1
                     /product="splicing factor 3A subunit 2"
                     /protein_id="NP_001011986.1"
                     /db_xref="GeneID:299620"
                     /db_xref="RGD:1308627"
                     /translation="MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDP
                     YFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKV
                     KVEVKKFVKIGRPGYKVTKQRDTEMGQQSLLFQIDYPEIAEGVMPRHRFMSAYEQRIE
                     PPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEKPP
                     APPSLPAGPPGVKRPPPPLMNGLPPRPPLPDALPPPPPGGLPLPPMPPTGPAPSGPPG
                     PPQMPPPAPGVHPPAPVVHPPTSGVHPPAPGVHPPAPVVHPPTSGVHPPAPGVHPPAP
                     GVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPPSAGVHPQAPG
                     VHPPAPAVHPQAPGVHPPAPGIHPQAPGVHPQPPPGVHPAAPGVHPQPPGVHPTPMPP
                     MLRPPLPSDGPGNMPPPPPGN"
     exon            208..279
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            280..326
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            327..436
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..486
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..627
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            628..696
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            697..1607
                     /gene="Sf3a2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggcattcgg ggaggttaag cccagttacc gaggccttgg tgttggtttc tcctgtcccc
       61 taaagtaccc aggcctgcat catggacttt cagcaccgac ctggaggcaa aactgggagt
      121 gggggcgtgg cctcctcctc agagagcaac cgggatcgaa gagagcgcct gcggcaactg
      181 gccctggaaa ccatcgacat caataaggac ccgtatttca tgaagaacca cctgggatcc
      241 tatgagtgca aactctgctt gactctgcat aacaatgagg ggagctacct ggcccatacc
      301 caagggaaga aacatcagac taacttggcc cggcgagctg ccaaggaggc caaagaagcc
      361 cccgcccagc ctgcaccaga gaaggtcaag gtggaggtga agaagtttgt gaagattggc
      421 cgccctggct acaaagtgac caagcagagg gacacagaga tgggccagca gagcctactg
      481 ttccagattg actaccctga gattgcagag ggtgtcatgc cccgccaccg cttcatgtct
      541 gcctatgagc agaggatcga gcccccggac cgccgctggc agtacctcct catggctgct
      601 gagccctatg agaccatcgc cttcaaggtg ccgagccgcg agatcgacaa ggctgagggc
      661 aagttttgga cccactggaa ccgagagacc aagcagtttt ttcttcagtt ccactttaag
      721 atggagaagc ccccagcccc tcccagcctc ccagcaggac ccccaggcgt caagcggccc
      781 ccgcccccac tcatgaacgg actacctcct cgcccaccac tccctgatgc cttgccccca
      841 cctccacctg gtggcctgcc tcttcctcct atgcccccca ctgggcctgc tccctctggg
      901 ccccctggac ctccccagat gcccccacca gctcctgggg tacacccccc agccccagta
      961 gtacacccac caacatctgg ggtccatccc ccagctcctg gggtgcaccc cccagcccca
     1021 gtggttcatc caccaacatc tggggtccat cccccagctc ctggggtcca tcctccagcc
     1081 cctggagtgc accctccagc ccctggagtg cacccaccag cccctggggt gcacccccca
     1141 gctccagggg tccacccccc agctccaggg gtccaccccc cagctccagg ggtccacccc
     1201 ccagctccag gggtccaccc tcctccatca gctggagttc atcctcaggc tccaggggtg
     1261 catccacctg ctcctgcagt ccaccctcaa gcccctggtg tacacccccc agctcctggg
     1321 atccaccctc aggcaccagg ggtgcacccc cagcctcctc ctggagtcca ccctgctgcc
     1381 ccaggggtcc atcctcagcc tccaggggtt caccctactc ctatgccccc catgctaagg
     1441 cccccccttc cctcggatgg ccctgggaac atgcctccgc ctcccccagg caactgagta
     1501 gcccgccacc agcctctgtg ctctgtgtgc tcttttccca tggccagtcc ttgggaggtt
     1561 ttctattttg tcctgtccta agtcaattaa acagcctccc ccatgtcaaa aaaaaaaaaa
     1621 aaaaaaaaaa aaa
//
