LOCUS       NM_012620               3069 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus serpin family E member 1 (Serpine1), mRNA.
ACCESSION   NM_012620 XM_346431
VERSION     NM_012620.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ishiwata,S., Iizuka,H., Sonoda,H., Tsunoda,D., Tajika,Y.,
            Chikuda,H., Koibuchi,N. and Shimokawa,N.
  TITLE     Upregulated miR-224-5p suppresses osteoblast differentiation by
            increasing the expression of Pai-1 in the lumbar spine of a rat
            model of congenital kyphoscoliosis
  JOURNAL   Mol Cell Biochem 475 (1-2), 53-62 (2020)
   PUBMED   32748313
  REMARK    GeneRIF: Upregulated miR-224-5p suppresses osteoblast
            differentiation by increasing the expression of Pai-1 in the lumbar
            spine of a rat model of congenital kyphoscoliosis.
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Li,H., Zhang,B., Lu,S., Ji,D.G., Ding,M., Ye,Y.S. and Sun,D.J.
  TITLE     siRNA-mediated silencing of PAI-1 gene acts as a promoter over the
            recanalization of endothelial progenitor cells in rats with venous
            thrombosis
  JOURNAL   J Cell Physiol 234 (11), 19921-19932 (2019)
   PUBMED   30982977
  REMARK    GeneRIF: PAI-1 gene silencing can promote the recanalization of
            venous thrombosis by enhancement of the luminal formation ability
            of rats' endothelial progenitor cells.
REFERENCE   3  (bases 1 to 3069)
  AUTHORS   Zhang,C., Ma,P., Zhao,Z., Jiang,N., Lian,D., Huo,P. and Yang,H.
  TITLE     miRNA-mRNA regulatory network analysis of mesenchymal stem cell
            treatment in cisplatin-induced acute kidney injury identifies roles
            for miR-210/Serpine1 and miR-378/Fos in regulating inflammation
  JOURNAL   Mol Med Rep 20 (2), 1509-1522 (2019)
   PUBMED   31257474
  REMARK    GeneRIF: miR210/Serpine1 and miR378/Fos play a prominent role in
            regulating inflammation in a cute kidney injury rat model.
REFERENCE   4  (bases 1 to 3069)
  AUTHORS   Chen,T., Huang,J.B., Dai,J., Zhou,Q., Raj,J.U. and Zhou,G.
  TITLE     PAI-1 is a novel component of the miR-17~92 signaling that
            regulates pulmonary artery smooth muscle cell phenotypes
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 315 (2), L149-L161 (2018)
   PUBMED   29644896
  REMARK    GeneRIF: These results suggest that miR-17~92 regulates the PASMC
            contractile phenotype and proliferation coordinately and
            synergistically by direct and indirect targeting of PAI-1.
REFERENCE   5  (bases 1 to 3069)
  AUTHORS   Liu,B., Wu,Y., Wang,Y., Cheng,Y., Yao,L., Liu,Y., Qian,H., Yang,H.
            and Shen,F.
  TITLE     NF-kappaB p65 Knock-down inhibits TF, PAI-1 and promotes activated
            protein C production in lipopolysaccharide-stimulated alveolar
            epithelial cells type II
  JOURNAL   Exp Lung Res 44 (4-5), 241-251 (2018)
   PUBMED   30449218
  REMARK    GeneRIF: Cells with NF-kappaB p65 knock-down showed significant
            decreases in TF, PAI-1, p65, p-p65, p-IkappaB expressions following
            LPS stimulation
REFERENCE   6  (bases 1 to 3069)
  AUTHORS   Seebacher,T., Manske,M., Zoller,J., Crabb,J. and Bade,E.G.
  TITLE     The EGF-inducible protein EIP-1 of migrating normal and malignant
            rat liver epithelial cells is identical to plasminogen activator
            inhibitor 1 and is a component of the ECM migration tracks
  JOURNAL   Exp Cell Res 203 (2), 504-507 (1992)
   PUBMED   1333984
REFERENCE   7  (bases 1 to 3069)
  AUTHORS   Hagege,J., Peraldi,M.N., Rondeau,E., Adida,C., Delarue,F.,
            Medcalf,R., Schleuning,W.D. and Sraer,J.D.
  TITLE     Plasminogen activator inhibitor-1 deposition in the extracellular
            matrix of cultured human mesangial cells
  JOURNAL   Am J Pathol 141 (1), 117-128 (1992)
   PUBMED   1632457
REFERENCE   8  (bases 1 to 3069)
  AUTHORS   Johnson,M.R., Bruzdzinski,C.J., Winograd,S.S. and Gelehrter,T.D.
  TITLE     Regulatory sequences and protein-binding sites involved in the
            expression of the rat plasminogen activator inhibitor-1 gene
  JOURNAL   J Biol Chem 267 (17), 12202-12210 (1992)
   PUBMED   1601887
REFERENCE   9  (bases 1 to 3069)
  AUTHORS   Allan,E.H., Hilton,D.J., Brown,M.A., Evely,R.S., Yumita,S.,
            Metcalf,D., Gough,N.M., Ng,K.W., Nicola,N.A. and Martin,T.J.
  TITLE     Osteoblasts display receptors for and responses to
            leukemia-inhibitory factor
  JOURNAL   J Cell Physiol 145 (1), 110-119 (1990)
   PUBMED   2170427
REFERENCE   10 (bases 1 to 3069)
  AUTHORS   Ehrlich,H.J., Gebbink,R.K., Keijer,J., Linders,M., Preissner,K.T.
            and Pannekoek,H.
  TITLE     Alteration of serpin specificity by a protein cofactor. Vitronectin
            endows plasminogen activator inhibitor 1 with thrombin inhibitory
            properties
  JOURNAL   J Biol Chem 265 (22), 13029-13035 (1990)
   PUBMED   1695900
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000227.1.
            
            [WARNING] On Mar 31, 2024 this sequence was replaced by
            NM_012620.3.
            
            On Nov 26, 2020 this sequence version replaced NM_012620.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M24067.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132292, SAMD00132294
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               JACYVU010000227.1  871012-871127
            117-388             JACYVU010000227.1  872179-872450
            389-622             JACYVU010000227.1  873739-873972
            623-817             JACYVU010000227.1  875449-875643
            818-1016            JACYVU010000227.1  876285-876483
            1017-1117           JACYVU010000227.1  878214-878314
            1118-1204           JACYVU010000227.1  878417-878503
            1205-1288           JACYVU010000227.1  879263-879346
            1289-3069           JACYVU010000227.1  879617-881397
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q12"
     gene            1..3069
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /note="serpin family E member 1"
                     /db_xref="GeneID:24617"
                     /db_xref="RGD:3249"
     exon            1..116
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            117..388
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1326
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /note="plasminogen activator inhibitor 1; PAI; PAI-1;
                     endothelial plasminogen activator inhibitor; serpin E1;
                     serine (or cysteine) proteinase inhibitor, member 1;
                     serine (or cysteine) peptidase inhibitor, clade E, member
                     1; serine (or cysteine) proteinase inhibitor, clade E,
                     member 1; serpin peptidase inhibitor, clade E (nexin,
                     plasminogen activator inhibitor type 1), member 1; serine
                     (or cysteine) proteinase inhibitor, clade E (nexin,
                     plasminogen activator inhibitor type 1), member 1"
                     /codon_start=1
                     /product="plasminogen activator inhibitor 1 precursor"
                     /protein_id="NP_036752.2"
                     /db_xref="GeneID:24617"
                     /db_xref="RGD:3249"
                     /translation="MQMSSALTCLTLGLVLVFGKGFASPLPESHTAQQATNFGVKVFQ
                     HVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLS
                     KELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEMERARFIIN
                     DWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGS
                     TISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNI
                     LDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSD
                     QEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTET
                     ILFMGQLMEP"
     exon            389..622
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            623..817
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            818..1016
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1117
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1204
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1288
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..3069
                     /gene="Serpine1"
                     /gene_synonym="Pai1; PAI1A; Pai1aa; Planh; RATPAI1A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccccgagagc tttgtgaagg aggaacgctg cacacccgcc tcccgcagca cacagccaac
       61 cacagctgag cgacagccaa caagagccaa tcacaaggca cttccgaaag ctccaggatg
      121 cagatgtctt cagccctcac ttgcctcacc ctgggcctgg ttctggtctt tgggaaaggg
      181 ttcgcttcac cccttccaga gtcccataca gcccagcagg ccaccaactt cggagtaaaa
      241 gtgtttcagc atgtggtcca ggcctccaaa gaccgaaatg tggtcttctc tccctacggc
      301 gtgtcctcgg tgctggctat gctgcagctg accacagcag ggaaaacccg gcagcagatc
      361 caagatgcta tgggattcaa tatcagtgag aggggcacag ctcctgccct ccgaaagctc
      421 tccaaggagc tcatggggtc atggaacaag aatgagatca gtactgcgga cgccatcttt
      481 gtccagcggg acctagagct ggtccagggc ttcatgcccc acttcttcaa gctcttccgg
      541 accacggtga agcaggtgga cttctcagag atggaaagag ccagattcat catcaacgac
      601 tgggtggaga ggcacaccaa aggtatgatc agtgacttac tggccaaggg ggctgtaaat
      661 gagctgacac gcctggtgct ggtgaacgcc ctctatttca acggccaatg gaagaccccc
      721 ttcttagagg ccagcaccca ccagcgcctg ttccacaagt ctgatggtag caccatctcc
      781 gtgcccatga tggctcagaa caacaagttc aactacactg agttcaccac tccggatggg
      841 cacgagtacg acatcctgga actgccctac cacggcgaaa ccctcagcat gttcattgca
      901 gcaccctttg aaaaagatgt gcccctctcc gccatcacca acattttgga cgctgagctc
      961 atcagacaat ggaagagcaa catgaccagg ctgccccgcc tcctcatcct gcctaagttc
     1021 tctctggaga ctgaagtgga cctcagaggg cccctggaga agctgggcat gactgacatc
     1081 ttcagctcaa cccaggccga cttcacaagt ctttccgacc aagagcagct ctctgtagca
     1141 caagcactac aaaaggtcaa gatcgaggtg aacgagagcg gcacagtggc gtcttcctcc
     1201 acagccattc tagtctcagc ccgcatggcc cccacggaga tggttttaga ccgatccttt
     1261 ctctttgtgg ttcggcacaa tccaacagag acaatcctct tcatgggcca gctgatggag
     1321 ccttgagagt gggatgagaa gccttccttt gggacaaaac tggacgtgtt ataagcagag
     1381 actctgaaga aaagaattgt tttaaggact ctttggggag aaagagaagg cctttctttc
     1441 ttaccccggc actggtaaat ctttccaacc agcctcccag acctcagact ctcgaagagg
     1501 aaagagtcta actccctcac tagggaccta tcttactaag gtctcatcca accatagaac
     1561 tcacagaatc tggatctgcc cagcattcag cctttggacc cagttcccac caaggcccca
     1621 gcagggccaa cccactacgc cttcactcag caaagtcttt tgtgttccag tcacactctg
     1681 ggtacctctt gtatcgtcct ccattgctat gaaggatgac ccaggccaaa ggaagaagca
     1741 ctgtcctatc tcaaggtcca ctgtggaaat gaacaccttg cccatcccca aggggcagca
     1801 gatagacaga tcgaatgatc gcccgatatc aagccttctc ccagctcccg tcctgccctc
     1861 ccttccctga cagcgcgctt gtgttatttc agagtgtaga tgacttgttt acagcttttt
     1921 tcgacccaca aacttttctc attttgaaag cgtgaaagaa aggtcagatg tgcacgtgcc
     1981 ttgctcttta tcctgggtct ccctgtgagg ggagaggggt cctggggaga ttccaggggt
     2041 gtgattgaat atttatcttg tttatcttat acgtttgttg gggagaagaa gcactattaa
     2101 ggagaaagcc ttttatttaa accatggcat atggtgtccc atttggggtc tgtatccctg
     2161 tatgtcaggg aggcatcact ccacaaaccc gcccctccgg gtggcccggc gtcggggctc
     2221 acactgccgc ctagtggcag ccgaacacgc ccttgcccca tccctccccc gcatcctccc
     2281 ccgtggctct tttccttagg gatcttgcca aggtgatgct tggcagccca cggtaaagga
     2341 agggggaaaa agattaggtg ggagagagag agagagaaag agagagagag agagagagag
     2401 agagagagag agagagagag agagagagag agagagagag agagagagat gtttgagaga
     2461 gggcaaagtg gtttcaaatt tttccaatac attcagaagc cgagtgggaa agggggctgt
     2521 gtgacctaac aggacagaac tttctccaat tactgggtga ctcagctgca ctggtgactc
     2581 acttcaatgt gtcatttccg gctgctgtaa gtgagcagtg gacacgtggg gggggggggg
     2641 gggggtgagg atgaaagaaa cagccagctc ctggtcaacc accttagtta gataatcttt
     2701 tttgaaagct tcctagctgg gggtatgatc agaaaaccaa tttactgaaa aactgcacag
     2761 gaaggtaacg tgaatctaat ttcatagcgg gccgctctgc atccgttaca tctccaccgg
     2821 aaaaaaataa tcattttctt tttttttgtg tgtgtgtgtg ttttagcttt tctccctctc
     2881 cctctttctc tctcatttca ttatgcactg gataaccata caccgtgtac ccacaggggc
     2941 cccaaatgtg gggtcacatg gtcttgaatt ttgtggggta catatgcctt tgtttgtttg
     3001 ttttcacttt tgatatataa acaggtaaat gtgtttttaa aaaataataa aaatagagaa
     3061 tatgcagac
//
