LOCUS       NM_001079889            2342 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus selenophosphate synthetase 2 (Sephs2), mRNA.
ACCESSION   NM_001079889 XM_001080177 XM_219347
VERSION     NM_001079889.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Hughes DJ, Kunicka T, Schomburg L, Liska V, Swan N and Soucek P.
  TITLE     Expression of Selenoprotein Genes and Association with Selenium
            Status in Colorectal Adenoma and Colorectal Cancer
  JOURNAL   Nutrients 10 (11), 1812 (2018)
   PUBMED   30469315
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Gladyshev VN, Arner ES, Berry MJ, Brigelius-Flohe R, Bruford EA,
            Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland
            PR, Diamond AM, Driscoll DM, Ferreiro A, Flohe L, Green FR, Guigo
            R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A,
            Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, Kohrle J, Krol A,
            Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV,
            Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP,
            Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U,
            Simonovic M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD
            and Zhang Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   3  (bases 1 to 2342)
  AUTHORS   Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R,
            Hatfield DL and Gladyshev VN.
  TITLE     Composition and evolution of the vertebrate and mammalian
            selenoproteomes
  JOURNAL   PLoS One 7 (3), e33066 (2012)
   PUBMED   22479358
REFERENCE   4  (bases 1 to 2342)
  AUTHORS   Xu XM, Carlson BA, Irons R, Mix H, Zhong N, Gladyshev VN and
            Hatfield DL.
  TITLE     Selenophosphate synthetase 2 is essential for selenoprotein
            biosynthesis
  JOURNAL   Biochem J 404 (1), 115-120 (2007)
   PUBMED   17346238
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC097429.1.
            
            On Apr 6, 2007 this sequence version replaced NM_001079889.1.
            
            Summary: This gene encodes an enzyme that catalyzes the production
            of monoselenophosphate (MSP) from selenide and ATP. MSP is the
            selenium donor required for synthesis of selenocysteine (Sec),
            which is co-translationally incorporated into selenoproteins at
            in-frame UGA codons that normally signal translation termination.
            The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop
            structure, the Sec insertion sequence (SECIS) element, which is
            necessary for the recognition of UGA as a Sec codon rather than as
            a stop signal. This protein is itself a selenoprotein containing a
            Sec residue at its active site, suggesting the existence of an
            autoregulatory mechanism. It is preferentially expressed in tissues
            implicated in the synthesis of selenoproteins and in sites of blood
            cell development. [provided by RefSeq, May 2017].
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC097429.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: inferred from conservation
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2342              BC097429.1         1-2342
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q37"
     gene            1..2342
                     /gene="Sephs2"
                     /note="selenophosphate synthetase 2"
                     /db_xref="GeneID:308993"
                     /db_xref="RGD:1308827"
     exon            1..2319
                     /gene="Sephs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             275..1630
                     /gene="Sephs2"
                     /EC_number="2.7.9.3"
                     /note="UGA stop codon recoded as selenocysteine"
                     /codon_start=1
                     /transl_except=(pos:458..460,aa:Sec)
                     /product="selenide, water dikinase 2"
                     /protein_id="NP_001073358.2"
                     /db_xref="GeneID:308993"
                     /db_xref="RGD:1308827"
                     /translation="MAEAAAGASGEAMAALVAAEGSLGPAGWSAGRSFSNYRPFEPQT
                     LGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSGLVGGQEETVQEG
                     GLTTRPGPGSAFPSLSIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANV
                     LSDLYAMGITECDNMLMLLSVSQSMSEKEREKVTPLMIKGFRDAAEEGGTAVTGGQTV
                     VNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEKWNK
                     IKMVVSREEVELAYQEAMFNMATLNRTAASLMHTFNAHAATDITGFGILGHSQNLAKQ
                     QKNEVSFVIHNLPIIAKMAAISKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEI
                     KSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGASASAAAAPDNSNAASEPS
                     S"
     regulatory      2134..2210
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Sephs2"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      2267..2272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs2"
     regulatory      2296..2301
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sephs2"
     polyA_site      2319
                     /gene="Sephs2"
ORIGIN      
        1 ggtgcaagca gacggttaat ttgttataca cctttttgaa taaaggaagc ccgcctctcg
       61 gtacgagaat gatcattgga tggagcgact gtcaatcatg ctgtatataa atacaggccg
      121 ctcggcgctc ggacaggcag cgtggcttga gtcttccgat cggtttctct tggtagatca
      181 tctccaggcc taacctttac ccaccgtggc aattagaatc gcctgcaggt accagggcct
      241 tctggttcgc acggcctccc gggcgggcgg tgcgatggcg gaagcggcgg caggcgccag
      301 cggagaagcc atggcggcac tagtggccgc ggaaggttcc ttgggcccgg cgggctggtc
      361 tgctggccgg agtttctcca actaccggcc gttcgagccc cagacactgg gcttcagccc
      421 gagctggcgg ctgacgagct tctccggcat gaaaggctga ggctgcaagg tcccccagga
      481 gaccctgctc aaactcctgg agggactgac gcggcccgcg ctgcagcccc cgcttacctc
      541 gggtctggtc gggggccagg aagagacggt gcaggaaggg ggcctgacca ccaggcccgg
      601 cccgggctca gccttcccct cgctgagcat tgggatggac tcctgcgtca tccccctgag
      661 gcacggaggc ctgtcgctgg tgcagaccac cgacttcttt taccccttgg tggaagatcc
      721 ctatatgatg gggcgcatag cttgtgccaa tgtgctcagt gacctctatg ccatgggcat
      781 taccgagtgt gacaacatgt tgatgttact cagtgtgagc cagagcatga gtgaaaagga
      841 acgagagaag gtaacgccgc tcatgatcaa aggctttcgt gacgctgcgg aagagggagg
      901 aactgcagtg actggtggac agacagtggt caacccttgg attatcatcg gtggggttgc
      961 cactgtggtg tgtcagcaaa atgaattcat aatgcctgac agcgctgtgg taggagatgt
     1021 gctggtatta accaagcctt taggaaccca ggttgctgcc aatgcccacc aatggctgga
     1081 taatcctgag aaatggaata aaatcaagat ggtggtttcc agagaggaag tagagctagc
     1141 ctatcaggaa gctatgttca acatggctac tctgaacagg actgctgcta gcttgatgca
     1201 cacttttaat gctcacgcag ccacggatat cacaggcttt ggcatattag gacactccca
     1261 gaacctcgca aaacagcaaa aaaatgaagt gtcctttgtc attcacaatc tgccaatcat
     1321 tgccaagatg gctgcgatca gcaaagccag tgggcgcttt ggcctcctcc aaggaacatc
     1381 agctgaaacc tctgggggat tactgatctg tctgccaaga gaacaggcgg cccgcttttg
     1441 ttcggaaatc aaatcttcca agtacggaga gggtcaccaa gcttggattg ttggcatcgt
     1501 ggagaaggga aaccggacag cccggatcat tgacaagcct cgcgttattg aagttctacc
     1561 tcggggagcc tctgcttctg ctgctgctgc tcctgacaat tccaatgcag cctctgagcc
     1621 tagttcttga aatggaatag cccttgttgg gaactcggag ccattctaca cgctcacaga
     1681 ctgttggcca gggttgattt taagaccttt ccaaaggctg ccggcatagt gcctctaggt
     1741 acttttgcta gttccatcaa aagctgcctg tgtacatcca gacccagaag tgcaggggat
     1801 gtgtgctcat ctgttgagag aatgaggagt aaaaaaacct tttcccaaag caagatgagg
     1861 ctattccagt tttagggatt ttttgcactg agttgattca tttctgcaca gggagtaaga
     1921 ttattaagat tacatacaga aaaaagtaaa ctgcaacatg aaaaaaatta tctggaccaa
     1981 catattgata agtctaaatt gttaggagaa ctcttactga tttattgtca aatttgtcat
     2041 taattttttt tctgagcaac tgcctctttt cctgttctga gcaagagttg agcaacttgt
     2101 ccagccagga aaggaaggcc atagccacct tacttggtct ctgataatga tgtttctccc
     2161 tctaactcct aataaggact gggagaggct gatcaaacct cagagccagg tgttggcggc
     2221 cgttaagata ctaaatctta cactgaaaat ttccagagat ttaattaata aaaaaaattt
     2281 cttaacagct aactaaataa aagggacttt gttggaaaaa aaaaaaaaaa aaaaaaaaaa
     2341 aa
//
