LOCUS       NM_001104630            5263 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus selenophosphate synthetase 1 (Sephs1), mRNA.
ACCESSION   NM_001104630 XM_001066465 XM_214509
VERSION     NM_001104630.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Hu,T., Shi,Z., Sun,Y., Hu,F., Rong,Y., Wang,J., Wang,L., Xu,W.,
            Zhang,F. and Zhang,W.Z.
  TITLE     SEPHS1 attenuates intervertebral disc degeneration by delaying
            nucleus pulposus cell senescence through the Hippo-Yap/Taz pathway
  JOURNAL   Am J Physiol Cell Physiol 326 (2), C386-C399 (2024)
   PUBMED   38105759
  REMARK    GeneRIF: SEPHS1 attenuates intervertebral disc degeneration by
            delaying nucleus pulposus cell senescence through the Hippo-Yap/Taz
            pathway.
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   3  (bases 1 to 5263)
  AUTHORS   Kim,J.Y., Lee,K.H., Shim,M.S., Shin,H., Xu,X.M., Carlson,B.A.,
            Hatfield,D.L. and Lee,B.J.
  TITLE     Human selenophosphate synthetase 1 has five splice variants with
            unique interactions, subcellular localizations and expression
            patterns
  JOURNAL   Biochem Biophys Res Commun 397 (1), 53-58 (2010)
   PUBMED   20471958
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY724491.1, DV714794.1, DY318079.1, DY313865.1, JAXUCZ010000017.1,
            CB704759.1 and BG375377.1.
            
            On or before Sep 16, 2007 this sequence version replaced
            XM_214509.4, XM_001066465.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY724491.1, SRR26643298.69876.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760433,
                                           SAMN12840107 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2925              AY724491.1         1-2925
            2926-3159           DV714794.1         381-614
            3160-3465           DY318079.1         309-614
            3466-4189           DY313865.1         74-797
            4190-4482           JAXUCZ010000017.1  78264488-78264780   c
            4483-4818           CB704759.1         13-348
            4819-5263           BG375377.1         1-445               c
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.3"
     gene            1..5263
                     /gene="Sephs1"
                     /note="selenophosphate synthetase 1"
                     /db_xref="GeneID:291314"
                     /db_xref="RGD:1311182"
     exon            1..283
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            284..557
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..1543
                     /gene="Sephs1"
                     /EC_number="2.7.9.3"
                     /codon_start=1
                     /product="selenide, water dikinase 1"
                     /protein_id="NP_001098100.1"
                     /db_xref="GeneID:291314"
                     /db_xref="RGD:1311182"
                     /translation="MSTRESFNPETYELDKSFRLTRFTELKGTGCKVPQDVLQKLLES
                     LQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRI
                     ACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVT
                     GGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIP
                     EKWNKIKLVVTQEDVELAYQEAMVNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQ
                     NLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAA
                     RFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS
                     "
     exon            558..661
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..769
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            770..924
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1015
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1115
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1328
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..5245
                     /gene="Sephs1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacggagccg ctccggccac cggcccgccg ccttaaaggg aggtggccgc tcttaaaggg
       61 accctcgcgc gcccggccgg cgggagcgcg gcggcccggt tcccggaggg ccgcacccag
      121 cctaggccga gctccacgcg gcgtccaagc gcggggccgt gccgccgcca ccgggagcag
      181 catttttatt caggcgacgc ttaagggagc ccggcgcgcc cggtgcattg tgggagcgcc
      241 gcggcccgtt ttcgggagga ggcggagggc gcaaagcgag ccggtggatc acaagtagaa
      301 agcccattgg atccagagaa agggagggga ggggctgaac tgtgaagagt gcggccccag
      361 aaccatgtct actcgggagt cctttaaccc ggaaacttat gaattggaca agagcttccg
      421 gctaaccagg tttactgaac tgaaaggcac aggctgcaaa gtgccccaag atgtcttgca
      481 gaaattgctg gaatctttac aagagaacca cttccaagaa gatgagcagt ttctgggagc
      541 ggttatgcca cgacttggca ttgggatgga tacttgcgtc attcctctga ggcacggtgg
      601 tctctccttg gttcaaacca cagattacat ttatcctatc gtggacgacc cttacatgat
      661 gggcaggata gcgtgtgcca atgtcctcag tgacctctat gcgatgggtg tcacagaatg
      721 tgacaatatg ctaatgctcc ttggagtcag taataaaatg actgaccggg aaagggataa
      781 agtgatgccg ctaattatac agggttttaa agatgcggcc gaggaagcgg ggacctctgt
      841 aacggggggc caaacggtat tgaacccctg gattgttctg ggaggagtcg ccacaactgt
      901 ctgccagccc aatgaattta tcatgccaga caacgcggtg ccgggggacg tgctggtgct
      961 gaccaaaccc ctggggacgc aggttgcagt ggctgtgcac cagtggctgg atattcctga
     1021 aaaatggaat aaaattaagc tcgtggtcac ccaagaagac gtagagctgg cataccaaga
     1081 ggcaatggtg aacatggcgc ggctcaacag gacagctgca ggcctcatgc acacgttcaa
     1141 tgctcatgca gccactgata tcacaggctt cgggattctg ggccatgcac agaacctggc
     1201 caagcaacag aggaacgagg tgtcgtttgt gattcacaat cttcccgtgc tagccaagat
     1261 ggccgctgtg agcaaagcct gcggaaacat gttcggccta atgcatggga cctgcccaga
     1321 gacgtcagga ggcctgctaa tctgcctacc gcgtgagcaa gcagctcggt tctgtgcaga
     1381 gataaagtcc cccaaatatg gcgaaggcca ccaagcatgg attattggga ttgtagagaa
     1441 gggcaaccgc acagccagaa tcatcgacaa acctcggatt attgaagtcg cacctcaagt
     1501 agccacgcaa aacgtgaatc ccacacctgg tgccacctcc taatccagac agaaatagct
     1561 attgattttg tctttacata gatctatttc ctttatcatc acttcaatta aagactctaa
     1621 gaacaacaaa tctcattgtg tctacacagc tggtgaccct aggtcgtttt atgagtggat
     1681 gcaattaata aaatgaaatc catggccttc ttttcctgtt acattaactg aagatgcacc
     1741 caacctccag gcagcttctg agttgagaat gatattgtta tccgataccg ttgattcatt
     1801 ttgaatcttt agatgcgcat ctcttgccgc acaggcgctc tctaaaggtg cttttctgcc
     1861 acaggcactg ctgagaggaa taccactagc agtctgtctt ctcattctac ctatcacatc
     1921 agtctggtct gttttcaagt gtctcggttg acactctaga aagacagaat tggtaagtca
     1981 cgtgacggcc tcccgtagtc agggttgcaa ggctccttcg agggtttggt ttgtaagttg
     2041 agtctaatac ctcgggggca tttcttcacc cagtagccca ctcggttcag gatggcaggt
     2101 gctttgcttg gcacggtaag cacgacttgg cagtaataac cgttgatggt ctttaccgta
     2161 ggagaaagga tactttctgc aatgttcata gttctatagc acgacaagga actgccttcg
     2221 ataggggctt cgtgtatagt actcatttat aattcagtat ctaatttact tcgcaaatga
     2281 tttttaaata tgatcaatac taaagactgt tctgtggatg gtagtgttta atacatttta
     2341 tattttgtat agtgatttca ggcctcttgt ttaaaatcag cagctcttta gcccaattct
     2401 tagcatcatt ttgtccttgc gcccgtacct ttttgtgcac gcattttgtg atttgtgtta
     2461 aaaacctgca ttgccaacgt tgcagctcca acataaaagt tgttattcaa ataaatattt
     2521 aattttttta attgctcttg tataatcaga tgcccttttt agtattattt tagaagcatc
     2581 tgggagggtt ttgcctaaaa tacaatttat tggggaaact agattttagt tttataaact
     2641 tttaagtctt tcatgggacc tatattttct tgaattaaat tttgtagttc tagaacaaat
     2701 aggcaatcta caaaggtgtt atctgtgttt cttaaaaaca ggcttcctta tcttccttat
     2761 ctgttaagca gctgggggat tcccgtcttt aagcacaagg gcactgggtc ctcaaatgcc
     2821 tgtcttcaaa agagaaaaag caccagtctt caggtttcac gggcttccta ctaacaggcc
     2881 ccaaagctaa gctttgtaaa ctcacccaac cttctcaaca cggaacacca ggaaaccacc
     2941 actggaccaa tggagcgccc agaaggaaga ggctgccaca tgcttgtgtc cctgggagcc
     3001 acgctacagc tggaacagaa ccaccgagtc tggccccaga gaggaagctg tcacagaagg
     3061 acactaattg gacactatag gaaatgatat gatgttggtc cttgaaagta tgcaccagtc
     3121 atctgcaaaa taaaacgtgt tggaaccctc tgagtgcaga aagcttactg taggacttac
     3181 ttctgtcttg ttttttctta aacagtaggt gaaattggag ctaactgtac attctgtccc
     3241 ccaaagccgc tacttttttt gtggcaagac tgtggagagg aaattagatg gctcatgtcc
     3301 tgagcaaatc atctttaaag attcatgtaa ggaggaaagg ccagggattt aagatccttg
     3361 gagttgtgat ctaggaaaag cccttgtctt gactagcatc acggtgctca gaaacccatg
     3421 aactagaatc acacaggcat ttgtgggggc attcctgccc ttccccaaga cccagagtca
     3481 attgtggcta gggaaccagg tcctccatct tgagaatgtc ccctctaggg ctttgcataa
     3541 taaaccttga gaaccgctga cctcaaaagg aatgttgggg catgtgtgta acaagtgggg
     3601 ctgtcaggga tccctttagt cttagccatt ccttttattt cctgtggtgc tctgtgtgtg
     3661 tgactctaaa gttcttgact caagtgactg tcattgttta gtaagtttgg caaagtctac
     3721 atggaactca atttatccaa tggtcactct tctaaaatcc acactggaca gtgctcctac
     3781 agaccctgga ccctgctggt gtccagtctt ctcatagaac aaaggaacat tgaagttcag
     3841 tgggttgttt ccacagctgt gttgaagcaa taacatactt gttctttttg gctcagtatt
     3901 ttgaaacctc caagtcttcc aaatttcgcg tgagttgtaa tgaataagtg gaagtgtttt
     3961 cattgttctg atgtgtgaac gagccccttg catcttccag cattgccttt aaaattgttc
     4021 tgagctggta agatggctca gtgggtacag tggcttgtct caaaagcctg agttccatcc
     4081 gcttccagaa cccatgtaga ggtgcaaaga aaagtggcct gcattgtgcg gcaaatgtac
     4141 ttatgcacct agcaataaat acattaatat ttagttgtca gagttctaga tcttcgtctt
     4201 attctgtaga aacagaaata atagctatac tggtttattt ttacagacgc ttagcacagg
     4261 caggagtttg cgagacctga tgatgggcat gcacacaacc ctgcctgaga tgttcattct
     4321 cacatctgac caggcgctca cacagcatac tcgcttccaa tagtcacgta tcctgttctc
     4381 attaagaatc cacattaagg attctagtca tgaaacccca gagaccacgg tgaaaatcta
     4441 gagtcctgtt gcaatttgat gatttgcctt tgtttaagtt gtgctttgaa tgagaccagg
     4501 aatgggtgga ttagaccagc cctgagtcaa gtcacttggg catttctagg cattcagaca
     4561 tgatcaagaa ccactggaag ccaaactcgt gcaatagagt ggttgtggtc tgtctggtga
     4621 gtatggggta tacaggtctg tgcactttcc tgtacttgat aggcctcaaa tgtcagcact
     4681 cttggtgttg atgggccttg ctaggtcgtg ctgcctttaa agggagttca atgctcagga
     4741 gaaagaagag catgtaatga agcccgccaa actatggggc tggacccaat gacacagggc
     4801 tgtaatccca tactcaggag gctgaagcaa ggggaccaca cgttcaaagt ctgcctgggc
     4861 tacccaataa agccctgttt tgctctccag cacaaaggat taagaaaagt ccgagagtac
     4921 agctcagttg tagagtactt gcctagcatt cacaaggtcc cgggtttagt ccacagtacc
     4981 agtaaaaacc aaacttgttg aaaacaagct taaaaggcac tagtttacta taattacgat
     5041 gtgatcatgg gtcagggctg atggagcctc cattattgta tttcaggaat aaaaacatgt
     5101 atgtggaaat atatagaatg taatgtttat caccccagca tattttgcac agttctaaag
     5161 caaaattata taaaattcga tgtctaggag atattcctgg gtttgggttc actttgggaa
     5221 ataaaactat aatagtttga acttcaaaaa aaaaaaaaaa aaa
//
