LOCUS       NM_133297               1537 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Rattus norvegicus selenoprotein F (Selenof), mRNA.
ACCESSION   NM_133297 XM_001079133
VERSION     NM_133297.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Dai,J., Zhou,J., Liu,H. and Huang,K.
  TITLE     Selenite and ebselen supplementation attenuates D-galactose-induced
            oxidative stress and increases expression of SELR and SEP15 in rat
            lens
  JOURNAL   J Biol Inorg Chem 21 (8), 1037-1046 (2016)
   PUBMED   27752786
  REMARK    GeneRIF: Data show that 15kD selenoprotein (SEP15) protein and
            selenoprotein R (SELR) protein expression was decreased in the
            lenses of galactose induced cataract.
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Gladyshev,V.N., Arner,E.S., Berry,M.J., Brigelius-Flohe,R.,
            Bruford,E.A., Burk,R.F., Carlson,B.A., Castellano,S., Chavatte,L.,
            Conrad,M., Copeland,P.R., Diamond,A.M., Driscoll,D.M., Ferreiro,A.,
            Flohe,L., Green,F.R., Guigo,R., Handy,D.E., Hatfield,D.L.,
            Hesketh,J., Hoffmann,P.R., Holmgren,A., Hondal,R.J., Howard,M.T.,
            Huang,K., Kim,H.Y., Kim,I.Y., Kohrle,J., Krol,A., Kryukov,G.V.,
            Lee,B.J., Lee,B.C., Lei,X.G., Liu,Q., Lescure,A., Lobanov,A.V.,
            Loscalzo,J., Maiorino,M., Mariotti,M., Sandeep Prabhu,K.,
            Rayman,M.P., Rozovsky,S., Salinas,G., Schmidt,E.E., Schomburg,L.,
            Schweizer,U., Simonovic,M., Sunde,R.A., Tsuji,P.A., Tweedie,S.,
            Ursini,F., Whanger,P.D. and Zhang,Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   3  (bases 1 to 1537)
  AUTHORS   Chavatte,L., Brown,B.A. and Driscoll,D.M.
  TITLE     Ribosomal protein L30 is a component of the UGA-selenocysteine
            recoding machinery in eukaryotes
  JOURNAL   Nat Struct Mol Biol 12 (5), 408-416 (2005)
   PUBMED   15821744
REFERENCE   4  (bases 1 to 1537)
  AUTHORS   Kyriakopoulos,A., Bukalis,K., Roethlein,D., Hoppe,B., Graebert,A.
            and Behne,D.
  TITLE     Prevention against oxidative stress of eukaryotic cell membranes by
            selenium compounds of the rat
  JOURNAL   Ann N Y Acad Sci 1030, 458-461 (2004)
   PUBMED   15659830
REFERENCE   5  (bases 1 to 1537)
  AUTHORS   Kumaraswamy,E., Korotkov,K.V., Diamond,A.M., Gladyshev,V.N. and
            Hatfield,D.L.
  TITLE     Genetic and functional analysis of mammalian Sep15 selenoprotein
  JOURNAL   Methods Enzymol 347, 187-197 (2002)
   PUBMED   11898406
  REMARK    GeneRIF: Genetic and functional analysis of mammalian Sep15
            selenoprotein.
REFERENCE   6  (bases 1 to 1537)
  AUTHORS   Korotkov,K.V., Kumaraswamy,E., Zhou,Y., Hatfield,D.L. and
            Gladyshev,V.N.
  TITLE     Association between the 15-kDa selenoprotein and
            UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic
            reticulum of mammalian cells
  JOURNAL   J Biol Chem 276 (18), 15330-15336 (2001)
   PUBMED   11278576
REFERENCE   7  (bases 1 to 1537)
  AUTHORS   Kumaraswamy,E., Malykh,A., Korotkov,K.V., Kozyavkin,S., Hu,Y.,
            Kwon,S.Y., Moustafa,M.E., Carlson,B.A., Berry,M.J., Lee,B.J.,
            Hatfield,D.L., Diamond,A.M. and Gladyshev,V.N.
  TITLE     Structure-expression relationships of the 15-kDa selenoprotein
            gene. Possible role of the protein in cancer etiology
  JOURNAL   J Biol Chem 275 (45), 35540-35547 (2000)
   PUBMED   10945981
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CK596377.1, BC060547.1,
            DY561321.1 and AI112281.1.
            
            On Aug 8, 2006 this sequence version replaced NM_133297.1.
            
            Summary: The protein encoded by this gene belongs to the
            SEP15/selenoprotein M family. The exact function of this protein is
            not known; however, it has been found to associate with
            UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic
            reticulum(ER)-resident protein, which is involved in the quality
            control of protein folding. The association with UGTR retains this
            protein in the ER, where it may play a role in protein folding.
            Knockout studies in mice also suggest a role for this gene in
            cataract formation and colon carcinogenesis. This protein is a
            selenoprotein, containing the rare amino acid selenocysteine (Sec).
            Sec is encoded by the UGA codon, which normally signals translation
            termination. The 3' UTRs of selenoprotein mRNAs contain a conserved
            stem-loop structure, designated the Sec insertion sequence (SECIS)
            element, that is necessary for the recognition of UGA as a Sec
            codon, rather than as a stop signal. [provided by RefSeq, Nov
            2016].
            
            ##Evidence-Data-START##
            Transcript exon combination :: CK596377.1, FQ212727.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: inferred from conservation
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-257               CK596377.1         16-272
            258-768             BC060547.1         7-517
            769-795             DY561321.1         507-533
            796-1482            BC060547.1         545-1231
            1483-1537           AI112281.1         1-55                c
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q44"
     gene            1..1537
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /note="selenoprotein F"
                     /db_xref="GeneID:113922"
                     /db_xref="RGD:621291"
     exon            1..351
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..765
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /note="UGA stop codon recoded as selenocysteine; 15 kDa
                     selenoprotein-like; selenoprotein 15"
                     /codon_start=1
                     /transl_except=(pos:553..555,aa:Sec)
                     /product="selenoprotein F precursor"
                     /protein_id="NP_579831.2"
                     /db_xref="GeneID:113922"
                     /db_xref="RGD:621291"
                     /translation="MAAGQGGWLRPALGLRLLLATAFQAVSALGAEFSSEACRELGFS
                     SNLLCSSCDLLGQFNLLPLDPVCRGCCQEEAQFETKKLYAGAILEVCGUKLGRFPQVQ
                     AFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLE
                     RI"
     exon            352..519
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /inference="alignment:Splign:2.1.0"
     exon            520..583
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /inference="alignment:Splign:2.1.0"
     exon            584..633
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /inference="alignment:Splign:2.1.0"
     exon            634..1521
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1345..1423
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Selenof"
                     /gene_synonym="Sep15"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1487..1492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Selenof"
                     /gene_synonym="Sep15"
     polyA_site      1521
                     /gene="Selenof"
                     /gene_synonym="Sep15"
ORIGIN      
        1 tgggacttcg tggatagccg ctgtaatgtc gtgaattaga agccccggtg tagggagtct
       61 aggaaagcgc ctgatttgaa tccctaaaag aacctgaggg ctgtttaata gccgatcact
      121 ttcaggcttc tgtagtcgac taactaacac tcgtctttgt ggagtaatag gcttgtagca
      181 gtggcctttt gcggcgggct tcgcatcagg cagtgcagtg gacgtccccg gcacaagggt
      241 cctcagactc ctaaagattg acggcagacc gcaaggatgg cggcagggca gggcgggtgg
      301 ctccggcccg cgctggggct tcgcttactg ctggcgactg cgtttcaagc ggtgtctgct
      361 cttggggcag agttctcgtc agaggcatgc cgggagttgg gcttctccag caacttgctc
      421 tgcagctcct gcgatctcct tggacagttt aacctgcttc cactggatcc tgtctgcaga
      481 ggctgctgtc aggaagaagc gcagtttgaa accaaaaagc tgtatgcagg agccatcctt
      541 gaagtctgtg gatgaaaatt ggggaggttc cctcaagtcc aagcttttgt cagaagcgat
      601 aaacccaaac tgttcagagg tctacagatc aagtatgttc gaggctcaga ccctgtacta
      661 aagcttttgg acgacaacgg gaacattgct gaagagctca gcatcctcaa gtggaacaca
      721 gacagtgtgg aagagttcct gagcgagaag ctggaacgca tataagccgt gctcagttgt
      781 ccgtctgtca cttaccacat gagctgtcac tgctcctagg aaacccctta gctttgctca
      841 cagtggtctc tgttttacgt gcaccatcac ttagttctgc atgcttacag tggtcatcgt
      901 ttatgtgcgg catcagtctt ctgattcgaa gctgaagtag ccccacagcc cataatgtta
      961 aggatctggc aaggttaaca aacccaactc ttagaaatct ctcttcttct gcacttgctg
     1021 atatcaccag tgaaatgctt tgtaaagagg catctggtta attatgctaa taatagttgg
     1081 tgataattgg tccagtttta caaacaacag aatttaaatg gaggagttaa taaaggaggt
     1141 gcctcccttg ctatgtgctc tttgaaagta agtgacagaa aactgcaaac cagcacgccc
     1201 gccccagagc ctggagctcc tgtagtgccc agctttcttt ttagcagaag tatttgtagt
     1261 ttgtaatgaa tgtgccacat acaattttta tagccttatt attacagaac agactgaaga
     1321 tctacagtaa gaatgtaata cttataaagg tttgcattaa tgaggattac acagaaaacc
     1381 tttgttaagg gtttgtgtcg atctgataat tggcaaattt ttatttttaa aatattctta
     1441 cagaagagtt ccatttaaga atgttcgtgt ataggaccaa aatataaata aaaactttca
     1501 aatatgtttg agtttaaatt aaaaaaaaaa aaaaaaa
//
