LOCUS       XM_008772805            6655 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus signal peptide, CUB domain and EGF
            like domain containing 3 (Scube3), transcript variant X1, mRNA.
ACCESSION   XM_008772805
VERSION     XM_008772805.3
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051355.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_008772805.4.
            
            On Jan 21, 2021 this sequence version replaced XM_008772805.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..6655
                     /gene="Scube3"
                     /note="signal peptide, CUB domain and EGF like domain
                     containing 3; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 18 ESTs, 4 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 14 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:294297"
                     /db_xref="RGD:1304880"
     CDS             92..3139
                     /gene="Scube3"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 3 isoform X1"
                     /protein_id="XP_008771027.1"
                     /db_xref="GeneID:294297"
                     /db_xref="RGD:1304880"
                     /translation="MHRHTGTLPLGNTPSKLTRDTSSVLMCLPLPGPDVDECVEGTDH
                     CHIDAICQNTPRSYKCICKSGYTGDGKHCKDVDECEREDNAGCVHDCVNIPGNYRCTC
                     YDGFHLAHDGHNCLDVDECAEGNGGCQQSCVNMMGSYECHCRDGFFLSDNQHTCIQRP
                     EEGMNCMNKNHGCAHICRETPKGGIACECRPGFELTKNQRDCKLTCNYGNGGCQHTCD
                     DTEQGPRCGCHVKFVLHTDGKTCIGERRLEQHGPTQAVSNETCAVNNGGCDSKCHDAA
                     TGVHCSCPVGFMLQPDRKTCKDIDECRLHNGGCDHICRNTVGSFECSCKKGYKLLINE
                     RSCQDIDECSFDRTCDHMCVNTPGSFQCLCHRGYLLYGISHCGDVDECSINKGGCRFG
                     CINTPGSYQCTCPAGQGRLHWNGKDCTEPVKCQSSSGASKAMLSCSRSGKKDTCALTC
                     PSRARFLPESENGFTVSCGTPSPKAAAGRAIHPGNSTISNSCHEAAVLPVKQRASFKI
                     KDAKCRLHLRNKGKAEEASGRTPGPGGASCSDCQVTFIHLKCDSSRKGKGRRARTPPG
                     KEVTRLTLELEAEVRAEETTAGCGLTCLRQRMERRLKGSLKMLRKSINQDRFLLRLAG
                     LDYELAHKPGLAAGDRAELVEVCRPGQHRAGTKCVSCPQGTYYHGQTEQCVPCPAGTF
                     QEREGQLSCDLCPGSDAHGPLGATNVTTCAGQCPPGQHSGDGFKPCQPCPRGTYQPEA
                     GRTLCFPCGGGLTTKHEGAVSFQDCDTKVQCSPGHYYNTSIHRCIRCAVGSYQPDFRQ
                     NFCTRCPGNTSTDFDGSTSVAQCKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNI
                     NPPPKRKILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSR
                     KLWINFKTSEANSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIK
                     AFFEVLAHPQNYFKYTEKHKEMLPKSFIKLLRSKVSSFLRPYK"
ORIGIN      
        1 tggagctgtc accatggttt gagtatttct gagcagtgag ggttcactcg aattcctgaa
       61 tcattcagag ggatgcaagg ggacctcagg catgcacagg catacgggga ctctgccttt
      121 ggggaacaca ccttcaaaac tcacccgaga caccagttct gttctgatgt gtcttcctct
      181 gcccgggcca gatgtagatg agtgtgtgga gggcacggat cactgtcaca ttgatgccat
      241 ctgccagaac accccacggt catacaagtg catctgcaag tccggctaca cgggggatgg
      301 caagcattgc aaagacgtgg atgagtgtga acgggaggac aacgctggct gtgtgcacga
      361 ctgtgtcaac atccctggta attaccggtg tacctgctac gacgggttcc acctggcaca
      421 cgacggacac aactgtctgg acgtggatga gtgtgcagag ggcaatgggg gctgccagca
      481 gagctgtgtc aacatgatgg gcagctatga atgccactgc cgagatggct tcttcctcag
      541 tgacaaccag cacacctgta tccagcggcc agaagaagga atgaactgca tgaacaagaa
      601 ccacggctgt gcccacattt gccgggagac tccgaagggg ggcattgcct gtgaatgccg
      661 ccctggcttc gagctcacca agaaccaacg ggactgtaaa ttgacctgca actacggtaa
      721 cggtggctgc cagcacacat gcgatgacac agagcagggt cctcggtgcg gctgccacgt
      781 caagtttgtg ctccatacgg acggaaagac atgcattggg gaaaggcgcc tagagcagca
      841 cgggcccact caggccgttt ctaatgagac ctgtgccgtc aacaacggag gctgtgacag
      901 taagtgtcac gatgcagcga ccggcgtcca ctgcagctgc cctgtgggtt tcatgctgca
      961 gccagacaga aagacctgca aagacataga cgagtgccgc ttacacaacg ggggctgtga
     1021 ccacatctgc cgcaacacag tgggcagctt tgaatgcagc tgtaagaagg gctacaagct
     1081 tctcatcaac gagaggagct gccaggatat agatgagtgt tcctttgatc gaacctgtga
     1141 ccacatgtgt gtcaacactc cgggaagctt ccagtgtctc tgtcatcgag gctaccttct
     1201 gtatggcatc tctcattgtg gggatgtgga tgaatgcagc atcaataagg ggggctgccg
     1261 ctttggctgc atcaacaccc caggcagcta tcagtgtacc tgcccggcag gccagggccg
     1321 cctccactgg aacggaaagg attgcacaga gccagtgaag tgccagagca gttctggagc
     1381 ctcaaaagcc atgctcagct gcagtcgctc gggcaagaag gatacctgtg ccctgacctg
     1441 cccctcccgc gcccggttct taccagagtc agagaacgga ttcacagtga gctgcggtac
     1501 cccgagccct aaagctgctg caggtcgagc cattcacccg ggcaacagca ccatctccaa
     1561 ctcctgccat gaggctgcag tgctgccagt caagcagagg gcctccttca agatcaagga
     1621 cgccaaatgt cgtctgcatc tgcgaaacaa aggcaaagcg gaggaggcca gcggcagaac
     1681 cccggggcca ggtggtgcct cctgctctga ctgccaggtc acctttatcc atcttaagtg
     1741 tgactcttct cggaagggca agggcagacg ggcccggacc cctccaggca aggaggttac
     1801 ccgactcacc ctggaattag aagctgaggt cagagcagaa gaaacgacag ctggctgtgg
     1861 gctgacttgc ctccgacagc ggatggaacg aaggctgaaa gggtccctga agatgctcag
     1921 aaagtccatc aaccaggacc ggttcctgct gcgtctggcc ggccttgact atgagctagc
     1981 ccacaagcca ggcctggcag ctggggaccg agcagagcta gtggaggtct gccgacccgg
     2041 gcagcaccga gcaggcacca agtgtgtcag ctgcccgcag ggaacgtatt accacggcca
     2101 gacggagcag tgtgtgccat gcccagcggg caccttccag gagagggaag ggcagctctc
     2161 ctgcgacctt tgccctggga gtgatgccca cgggcctctt ggagccacca acgtcaccac
     2221 gtgtgcaggt cagtgcccac ctggtcaaca ctcaggggat ggatttaagc cctgccagcc
     2281 ctgcccacgt ggcacctatc agcccgaagc aggacgaacc ctgtgcttcc cctgtggcgg
     2341 aggcctcact accaagcacg agggggcagt ctccttccag gactgtgaca ccaaagtcca
     2401 gtgctccccg ggccactact acaacactag catccatcgc tgtatccgct gtgctgtggg
     2461 ctcctaccag ccagacttcc gccagaactt ctgcacccgc tgcccaggaa atacaagcac
     2521 ggacttcgat ggctctacca gtgtggccca gtgcaagaat aggcagtgtg gcggggagct
     2581 gggtgagttc actggctaca tcgagtctcc caactacccg ggtaactacc cagctggtgt
     2641 ggagtgcatc tggaacatca accccccacc caagcgcaag atcctcattg tggtccctga
     2701 aatcttcctg ccgtccgagg acgagtgtgg ggacgtcctg gtcatgagaa agaactcctc
     2761 cccatcctcc atcaccacgt atgagacctg ccagacatac gagcggccca tcgccttcac
     2821 cgcccggtcc aggaaactct ggatcaactt caagaccagc gaggccaaca gcgcgcgtgg
     2881 ttttcagatt ccctatgtaa cctacgatga ggactatgag cagctggtgg aagatatagt
     2941 gcgggatggc cggctgtacg cctctgagaa ccaccaggag atcctaaagg ataagaagct
     3001 tatcaaggcc ttctttgagg tcctggccca cccccaaaac tacttcaagt acacagagaa
     3061 acacaaggag atgctgccaa agtccttcat caagttactg cgctccaagg tctccagctt
     3121 cctgaggcct tacaaatagt gcctgtgggc tccgagaccc gggtttcaga gctcccacct
     3181 ccctggccct cagagctggc accccgacct cagacaacaa actgtcttct cctcttggag
     3241 gagcgatacc acgacaccag gcactctccc cttctgtctt tctagttacc ttgccttcct
     3301 tctgtctctc tcctggaaaa gtcattcagt gctgtctgtt ccccccaggg gtgaaggaat
     3361 agacaccctc tacccctctg ccattatact gcggtgcctt gctagccagg agccaaaaga
     3421 ttcctccctc agcagcctgt gatccccaga gtgactcttc ccagcccaga gtcctgccaa
     3481 agaagccttt gacatggcag gtttatcccg actgggctgc tggggcacac actttgagcc
     3541 ctggggtgcc cacacagttg gtgtcctcat ctgtggagct ccttgctctg actggggctg
     3601 actccgtagg gtttacaagt ttccatactg ctgggctggc aaacccctgc cagggaaggg
     3661 agaacagcag gcaggggccc cagacataca aagctctcag atagctctgc aggacagaaa
     3721 gcagcttttc aaaacattgc ccaaaacaaa ggaaagaaaa tgtattgcct aaaggagctc
     3781 cctgcagaac aagtagaagc cagcctcaaa cactaaccct ttccttgcca gccctctgtg
     3841 ccagctgtgc cctcacccta cctaagggct ccctggggag cctgttaagt gctgtcccta
     3901 gagaaacctg gctgttggct ctccgggtct ggaccttgct ccacggttgt ttttcccttg
     3961 tagccccagg tggcttgtgg gctccagcaa agaggatggg ctctgcagtc agcctggagc
     4021 tgcctacctc tagcttcagg ctgtgggcag caggaggagt gagtgagtgc ccttggcaag
     4081 ctgcctgccc accctctcca catctgagaa gtgcaatcat tttgagtctt tctgtgttgt
     4141 ctagagggag gggttttttg tttgtttctt tttcctctta gcttagccct atttatagct
     4201 gcccaggagg gctgtgttgg gagtggagga acagcagtct gactgtcagc agccttaaga
     4261 gccagagcat ctcatctgtc tctgtcctag ctgaggccgc ccagaacttc agagccctcg
     4321 gctgtgcaag ccctcagagg cgcccaaggc accggagacc agctccgtaa agtgatgggg
     4381 cgggaggaag aggcttcctg cctgagccaa tgctgccttc cttgtgtctg tctctgtgga
     4441 ttcgtggtca cagatgcaga tggactggtc aggaatctcc tgcgagtagg gacaacctgt
     4501 cttcctgctt catgctctct cctgacatac catggaagag catcaggatg gccagcgaaa
     4561 gctcccagga cgtccagagg ctggagcagc ctcaacttgg atagttagcc atgtatgaag
     4621 ctggtgaagg aacaagaagc agacatgtgc ctgacactgg ctgcctgcct cctggagctg
     4681 actcaagcct gtttcgacta ctgtttcaaa aggaagaaag gcgtctgtgc ccgggcccag
     4741 gctctaccca tgctctaccc ttaacccagt tgctgcctgt aaagtctcca attgttcctt
     4801 ctccttcccc tgaggctgga gtcctggaca gccaccgtga gtcctcaggt ccaactctag
     4861 gtaactgcca agagtttcaa ctaccaaaaa gtctgatcct cacacacaca gcaaacaagg
     4921 ccagcttccg ggacacatta gccaacatgg tcagggtggg gtgggggtca gaggctattg
     4981 taaagctgtt aaatggcttc atacaaaggg gtgactgggc atttagaggg atgtctgtgc
     5041 ttccccagaa gcgctccaga acagcctgga tcaactctga tcgtcctaat aggagcatgg
     5101 ataactgcag atcctctctc cctgccattc tgcccagctc ctggaaagcc acaccgtgac
     5161 agtgcctccc aatgtgtgcc tttagggtgt agcttctctg cattcccgtc acacatcaca
     5221 catgcacaga catttcctct aatcatttac tctatggctg atggagtggc gcatgagatc
     5281 caccatacgg agagagcagc cactagactt agctggttaa tggagagagc agccactaga
     5341 cttagctggt taatggagag agcagccact agacttagct gatgtcaagg aggatggagg
     5401 agatcaagcg agcacttgag ctgtcaaccg agttggcagt taggaatcca ggcagagcta
     5461 cagaggatcc tggccctgag gcaccaaccg agttggcagt taggaatcca ggcagagctt
     5521 acagaggatc ctggccctga ggcaccaact ctcggggctc agtggactga atgaggagtt
     5581 aggaactcag ggactttcta ggactgcaca ggtagaacga aagaggtttc caggaggaag
     5641 tgggaggctg gggagacggt gtgaacagga acccttcgtg acctgaccca gaagcttctc
     5701 tgtgagctct ggtgcttttg gggtcacata actgtggcac ctcatccatc ctagcacgag
     5761 acctgccctg aactgagtcc cagtgtggga atccacttcc acaagcgagt gaccgtcaag
     5821 caggcacctc gacgcctctt tagccctctg cctctatttg tgtcgtcagg cctgaggtaa
     5881 caggagaggt ccgaccagag cctggacttt agctgcccac cctatgccta cggtgaccct
     5941 ttgaggactg ccatcgggcc tccctctgct catttccccg ggaggtggag aggacaggtt
     6001 tattgcttca agttcagtgg tacggtctgg gctctggctc ctatgagtct ttgtgaatct
     6061 gtgaagggtt ctccatgtgc atgtctgagg tgactcttaa atggattagg aagtcgtatc
     6121 tcggtgtgta agagaaagct atcgcacatt taagggtctg tgtttgctcc tgagcttcaa
     6181 ggcaggagag cagagcatgt taccccacct ggttcctttc ttaagcagac atcacgaagg
     6241 aaaccttgac attggacctg tctcaaagct ggcacactct gtctttctgc cattatttcg
     6301 ttcgactagg aatcctttga gagctagaag gactagaagc ccagttctgc gatcagcttc
     6361 ctgagacaaa caagctcaaa actttgttct atgtaagttg gaactttcag agaataatta
     6421 tcacgtggat ggcttttttt taatgcaaaa gaaaaagatc tcaaaatact gtatttatat
     6481 ctgccttcta ccgcctccca cacagcgagc atctcagcca acaacagaca gcagcaatgt
     6541 ggtacacaga gtattttgca cttgggggaa aaatatgtat ctgaacttgt aaaaaggaat
     6601 gtttggattt tgtattgtct tttttatatt attaaaatac taaatgaaac tccga
//
