LOCUS       NM_001271362            6718 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus signal peptide, CUB domain and EGF like domain
            containing 3 (Scube3), mRNA.
ACCESSION   NM_001271362 XM_001078282
VERSION     NM_001271362.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Lin,Y.C., Roffler,S.R., Yan,Y.T. and Yang,R.B.
  TITLE     Disruption of Scube2 Impairs Endochondral Bone Formation
  JOURNAL   J Bone Miner Res 30 (7), 1255-1267 (2015)
   PUBMED   25639508
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Wu,B.T., Su,Y.H., Tsai,M.T., Wasserman,S.M., Topper,J.N. and
            Yang,R.B.
  TITLE     A novel secreted, cell-surface glycoprotein containing multiple
            epidermal growth factor-like repeats and one CUB domain is highly
            expressed in primary osteoblasts and bones
  JOURNAL   J Biol Chem 279 (36), 37485-37490 (2004)
   PUBMED   15234972
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000020.1.
            
            On Sep 17, 2022 this sequence version replaced NM_001271362.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN11044071,
                              SAMN16093042 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-302               JAXUCZ010000020.1  6201066-6201367
            303-425             JAXUCZ010000020.1  6210930-6211052
            426-551             JAXUCZ010000020.1  6211665-6211790
            552-686             JAXUCZ010000020.1  6213837-6213971
            687-812             JAXUCZ010000020.1  6214724-6214849
            813-929             JAXUCZ010000020.1  6215029-6215145
            930-1046            JAXUCZ010000020.1  6218739-6218855
            1047-1169           JAXUCZ010000020.1  6220090-6220212
            1170-1286           JAXUCZ010000020.1  6220785-6220901
            1287-1412           JAXUCZ010000020.1  6221592-6221717
            1413-1529           JAXUCZ010000020.1  6221962-6222078
            1530-1634           JAXUCZ010000020.1  6222212-6222316
            1635-1754           JAXUCZ010000020.1  6222531-6222650
            1755-1913           JAXUCZ010000020.1  6222898-6223056
            1914-2129           JAXUCZ010000020.1  6223261-6223476
            2130-2291           JAXUCZ010000020.1  6223816-6223977
            2292-2459           JAXUCZ010000020.1  6224184-6224351
            2460-2621           JAXUCZ010000020.1  6224826-6224987
            2622-2819           JAXUCZ010000020.1  6225295-6225492
            2820-2972           JAXUCZ010000020.1  6225859-6226011
            2973-3052           JAXUCZ010000020.1  6226111-6226190
            3053-6718           JAXUCZ010000020.1  6229183-6232848
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..6718
                     /gene="Scube3"
                     /note="signal peptide, CUB domain and EGF like domain
                     containing 3"
                     /db_xref="GeneID:294297"
                     /db_xref="RGD:1304880"
     exon            1..302
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..3202
                     /gene="Scube3"
                     /note="signal peptide, CUB and EGF-like domain-containing
                     protein 3; signal peptide, CUB domain, EGF-like 3"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 3 precursor"
                     /protein_id="NP_001258291.1"
                     /db_xref="GeneID:294297"
                     /db_xref="RGD:1304880"
                     /translation="MGSGRVPGLCLLVLLVHARAAQHGKAAQDVDECVEGTDHCHIDA
                     ICQNTPRSYKCICKSGYTGDGKHCKDVDECEREDNAGCVHDCVNIPGNYRCTCYDGFH
                     LAHDGHNCLDVDECAEGNGGCQQSCVNMMGSYECHCRDGFFLSDNQHTCIQRPEEGMN
                     CMNKNHGCAHICRETPKGGIACECRPGFELTKNQRDCKLTCNYGNGGCQHTCDDTEQG
                     PRCGCHVKFVLHTDGKTCIETCAVNNGGCDSKCHDAATGVHCSCPVGFMLQPDRKTCK
                     DIDECRLHNGGCDHICRNTVGSFECSCKKGYKLLINERSCQDIDECSFDRTCDHMCVN
                     TPGSFQCLCHRGYLLYGISHCGDVDECSINKGGCRFGCINTPGSYQCTCPAGQGRLHW
                     NGKDCTEPVKCQSSSGASKAMLSCSRSGKKDTCALTCPSRARFLPESENGFTVSCGTP
                     SPKAAAGRAIHPGNSTISNSCHEAAVLPVKQRASFKIKDAKCRLHLRNKGKAEEASGR
                     TPGPGGASCSDCQVTFIHLKCDSSRKGKGRRARTPPGKEVTRLTLELEAEVRAEETTA
                     GCGLTCLRQRMERRLKGSLKMLRKSINQDRFLLRLAGLDYELAHKPGLAAGDRAELVE
                     VCRPGQHRAGTKCVSCPQGTYYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPL
                     GATNVTTCAGQCPPGQHSGDGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAVS
                     FQDCDTKVQCSPGHYYNTSIHRCIRCAVGSYQPDFRQNFCTRCPGNTSTDFDGSTSVA
                     QCKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSED
                     ECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPY
                     VTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKE
                     MLPKSFIKLLRSKVSSFLRPYK"
     exon            303..425
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            426..551
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            552..686
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            687..812
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            813..929
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1046
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1169
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1286
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1412
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1529
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1634
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1754
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1913
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2129
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2291
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2459
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2621
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2819
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2972
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3052
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..6718
                     /gene="Scube3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctacagct ccccgcgggc gaagctgggc gtcgggtgcg atagcccggg ccgcccctcg
       61 ggccgccgcc ccgcgccagc ccgcgctccc caccgcgcgc tccccacgcc cagcccggag
      121 cctccgcggg gcgccctcca gtcccctccc cctccgcctc gcgccctccc cggggctgcg
      181 acacccccgg cggccccgcc gcgcggagct ccaggccatg ggctcggggc gcgtccccgg
      241 gctctgcctg ctcgtcctgc tggtccacgc tcgcgccgcc cagcatggca aagctgcgca
      301 agatgtagat gagtgtgtgg agggcacgga tcactgtcac attgatgcca tctgccagaa
      361 caccccacgg tcatacaagt gcatctgcaa gtccggctac acgggggatg gcaagcattg
      421 caaagacgtg gatgagtgtg aacgggagga caacgctggc tgtgtgcacg actgtgtcaa
      481 catccctggt aattaccggt gtacctgcta cgacgggttc cacctggcac acgacggaca
      541 caactgtctg gacgtggatg agtgtgcaga gggcaatggg ggctgccagc agagctgtgt
      601 caacatgatg ggcagctatg aatgccactg ccgagatggc ttcttcctca gtgacaacca
      661 gcacacctgt atccagcggc cagaagaagg aatgaactgc atgaacaaga accacggctg
      721 tgcccacatt tgccgggaga ctccgaaggg gggcattgcc tgtgaatgcc gccctggctt
      781 cgagctcacc aagaaccaac gggactgtaa attgacctgc aactacggta acggtggctg
      841 ccagcacaca tgcgatgaca cagagcaggg tcctcggtgc ggctgccacg tcaagtttgt
      901 gctccatacg gacggaaaga catgcattga gacctgtgcc gtcaacaacg gaggctgtga
      961 cagtaagtgt cacgatgcag cgaccggcgt ccactgcagc tgccctgtgg gtttcatgct
     1021 gcagccagac agaaagacct gcaaagacat agacgagtgc cgcttacaca acgggggctg
     1081 tgaccacatc tgccgcaaca cagtgggcag ctttgaatgc agctgtaaga agggctacaa
     1141 gcttctcatc aacgagagga gctgccagga tatagatgag tgttcctttg atcgaacctg
     1201 tgaccacatg tgtgtcaaca ctccgggaag cttccagtgt ctctgtcatc gaggctacct
     1261 tctgtatggc atctctcatt gtggggatgt ggatgaatgc agcatcaata aggggggctg
     1321 ccgctttggc tgcatcaaca ccccaggcag ctatcagtgt acctgcccgg caggccaggg
     1381 ccgcctccac tggaacggaa aggattgcac agagccagtg aagtgccaga gcagttctgg
     1441 agcctcaaaa gccatgctca gctgcagtcg ctcgggcaag aaggatacct gtgccctgac
     1501 ctgcccctcc cgcgcccggt tcttaccaga gtcagagaac ggattcacag tgagctgcgg
     1561 taccccgagc cctaaagctg ctgcaggtcg agccattcac ccgggcaaca gcaccatctc
     1621 caactcctgc catgaggctg cagtgctgcc agtcaagcag agggcctcct tcaagatcaa
     1681 ggacgccaaa tgtcgtctgc atctgcgaaa caaaggcaaa gcggaggagg ccagcggcag
     1741 aaccccgggg ccaggtggtg cctcctgctc tgactgccag gtcaccttta tccatcttaa
     1801 gtgtgactct tctcggaagg gcaagggcag acgggcccgg acccctccag gcaaggaggt
     1861 tacccgactc accctggaat tagaagctga ggtcagagca gaagaaacga cagctggctg
     1921 tgggctgact tgcctccgac agcggatgga acgaaggctg aaagggtccc tgaagatgct
     1981 cagaaagtcc atcaaccagg accggttcct gctgcgtctg gccggccttg actatgagct
     2041 agcccacaag ccaggcctgg cagctgggga ccgagcagag ctagtggagg tctgccgacc
     2101 cgggcagcac cgagcaggca ccaagtgtgt cagctgcccg cagggaacgt attaccacgg
     2161 ccagacggag cagtgtgtgc catgcccagc gggcaccttc caggagaggg aagggcagct
     2221 ctcctgcgac ctttgccctg ggagtgatgc ccacgggcct cttggagcca ccaacgtcac
     2281 cacgtgtgca ggtcagtgcc cacctggtca acactcaggg gatggattta agccctgcca
     2341 gccctgccca cgtggcacct atcagcccga agcaggacga accctgtgct tcccctgtgg
     2401 cggaggcctc actaccaagc acgagggggc agtctccttc caggactgtg acaccaaagt
     2461 ccagtgctcc ccgggccact actacaacac tagcatccat cgctgtatcc gctgtgctgt
     2521 gggctcctac cagccagact tccgccagaa cttctgcacc cgctgcccag gaaatacaag
     2581 cacggacttc gatggctcta ccagtgtggc ccagtgcaag aataggcagt gtggcgggga
     2641 gctgggtgag ttcactggct acatcgagtc tcccaactac ccgggtaact acccagctgg
     2701 tgtggagtgc atctggaaca tcaacccccc acccaagcgc aagatcctca ttgtggtccc
     2761 tgaaatcttc ctgccgtccg aggacgagtg tggggacgtc ctggtcatga gaaagaactc
     2821 ctccccatcc tccatcacca cgtatgagac ctgccagaca tacgagcggc ccatcgcctt
     2881 caccgcccgg tccaggaaac tctggatcaa cttcaagacc agcgaggcca acagcgcgcg
     2941 tggttttcag attccctatg taacctacga tgaggactat gagcagctgg tggaagatat
     3001 agtgcgggat ggccggctgt acgcctctga gaaccaccag gagatcctaa aggataagaa
     3061 gcttatcaag gccttctttg aggtcctggc ccacccccaa aactacttca agtacacaga
     3121 gaaacacaag gagatgctgc caaagtcctt catcaagtta ctgcgctcca aggtctccag
     3181 cttcctgagg ccttacaaat agtgcctgtg ggctccgaga cccgggtttc agagctccca
     3241 cctccctggc cctcagagct ggcaccccga cctcagacaa caaactgtct tctcctcttg
     3301 gaggagcgat accacgacac caggcactct ccccttctgt ctttctagtt accttgcctt
     3361 ccttctgtct ctctcctgga aaagtcattc agtgctgtct gttcccccca ggggtgaagg
     3421 aatagacacc ctctacccct ctgccattat actgcggtgc cttgctagcc aggagccaaa
     3481 agattcctcc ctcagcagcc tgtgatcccc agagtgactc ttcccagccc agagtcctgc
     3541 caaagaagcc tttgacatgg caggtttatc ccgactgggc tgctggggca cacactttga
     3601 gccctggggt gcccacacag ttggtgtcct catctgtgga gctccttgct ctgactgggg
     3661 ctgactccgt agggtttaca agtttccata ctgctgggct ggcaaacccc tgccagggaa
     3721 gggagaacag caggcagggg ccccagacat acaaagctct cagatagctc tgcaggacag
     3781 aaagcagctt ttcaaaacat tgcccaaaac aaaggaaaga aaatgtattg cctaaaggag
     3841 ctccctgcag aacaagtaga agccagcctc aaacactaac cctttccttg ccagccctct
     3901 gtgccagctg tgccctcacc ctacctaagg gctccctggg gagcctgtta agtgctgtcc
     3961 ctagagaaac ctggctgttg gctctccggg tctggacctt gctccacggt tgtttttccc
     4021 ttgtagcccc aggtggcttg tgggctccag caaagaggat gggctctgca gtcagcctgg
     4081 agctgcctac ctctagcttc aggctgtggg cagcaggagg agtgagtgag tgcccttggc
     4141 aagctgcctg cccaccctct ccacatctga gaagtgcaat cattttgagt ctttctgtgt
     4201 tgtctagagg gaggggtttt ttgtttgttt ctttttcctc ttagcttagc cctatttata
     4261 gctgcccagg agggctgtgt tgggagtgga ggaacagcag tctgactgtc agcagcctta
     4321 agagccagag catctcatct gtctctgtcc tagctgaggc cgcccagaac ttcagagccc
     4381 tcggctgtgc aagccctcag aggcgcccaa ggcaccggag accagctccg taaagtgatg
     4441 gggcgggagg aagaggcttc ctgcctgagc caatgctgcc ttccttgtgt ctgtctctgt
     4501 ggattcgtgg tcacagatgc agatggactg gtcaggaatc tcctgcgagt agggacaacc
     4561 tgtcttcctg cttcatgctc tctcctgaca taccatggaa gagcatcagg atggccagcg
     4621 aaagctccca ggacgtccag aggctggagc agcctcaact tggatagtta gccatgtatg
     4681 aagctggtga aggaacaaga agcagacatg tgcctgacac tggctgcctg cctcctggag
     4741 ctgactcaag cctgtttcga ctactgtttc aaaaggaaga aaggcgtctg tgcccgggcc
     4801 caggctctac ccatgctcta cccttaaccc agttgctgcc tgtaaagtct ccaattgttc
     4861 cttctccttc ccctgaggct ggagtcctgg acagccaccg tgagtcctca ggtccaactc
     4921 taggtaactg ccaagagttt caactaccaa aaagtctgat cctcacacac acagcaaaca
     4981 aggccagctt ccgggacaca ttagccaaca tggtcagggt ggggtggggg tcagaggcta
     5041 ttgtaaagct gttaaatggc ttcatacaaa ggggtgactg ggcatttaga gggatgtctg
     5101 tgcttcccca gaagcgctcc agaacagcct ggatcaactc tgatcgtcct aataggagca
     5161 tggataactg cagatcctct ctccctgcca ttctgcccag ctcctggaaa gccacaccgt
     5221 gacagtgcct cccaatgtgt gcctttaggg tgtagcttct ctgcattccc gtcacacatc
     5281 acacatgcac agacatttcc tctaatcatt tactctatgg ctgatggagt ggcgcatgag
     5341 atccaccata cggagagagc agccactaga cttagctggt taatggagag agcagccact
     5401 agacttagct ggttaatgga gagagcagcc actagactta gctgatgtca aggaggatgg
     5461 aggagatcaa gcgagcactt gagctgtcaa ccgagttggc agttaggaat ccaggcagag
     5521 ctacagagga tcctggccct gaggcaccaa ccgagttggc agttaggaat ccaggcagag
     5581 cttacagagg atcctggccc tgaggcacca actctcgggg ctcagtggac tgaatgagga
     5641 gttaggaact cagggacttt ctaggactgc acaggtagaa cgaaagaggt ttccaggagg
     5701 aagtgggagg ctggggagac ggtgtgaaca ggaacccttc gtgacctgac ccagaagctt
     5761 ctctgtgagc tctggtgctt ttggggtcac ataactgtgg cacctcatcc atcctagcac
     5821 gagacctgcc ctgaactgag tcccagtgtg ggaatccact tccacaagcg agtgaccgtc
     5881 aagcaggcac ctcgacgcct ctttagccct ctgcctctat ttgtgtcgtc aggcctgagg
     5941 taacaggaga ggtccgacca gagcctggac tttagctgcc caccctatgc ctacggtgac
     6001 cctttgagga ctgccatcgg gcctccctct gctcatttcc ccgggaggtg gagaggacag
     6061 gtttattgct tcaagttcag tggtacggtc tgggctctgg ctcctatgag tctttgtgaa
     6121 tctgtgaagg gttctccatg tgcatgtctg aggtgactct taaatggatt aggaagtcgt
     6181 atctcggtgt gtaagagaaa gctatcgcac atttaagggt ctgtgtttgc tcctgagctt
     6241 caaggcagga gagcagagca tgttacccca cctggttcct ttcttaagca gacatcacga
     6301 aggaaacctt gacattggac ctgtctcaaa gctggcacac tctgtctttc tgccattatt
     6361 tcgttcgact aggaatcctt tgagagctag aaggactaga agcccagttc tgcgatcagc
     6421 ttcctgagac aaacaagctc aaaactttgt tctatgtaag ttggaacttt cagagaataa
     6481 ttatcacgtg gatggctttt ttttaatgca aaagaaaaag atctcaaaat actgtattta
     6541 tatctgcctt ctaccgcctc ccacacagcg agcatctcag ccaacaacag acagcagcaa
     6601 tgtggtacac agagtatttt gcacttgggg gaaaaatatg tatctgaact tgtaaaaagg
     6661 aatgtttgga ttttgtattg tcttttttat attattaaaa tactaaatga aactccga
//
