LOCUS       XM_003748970            4172 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus signal peptide, CUB domain and EGF
            like domain containing 2 (Scube2), transcript variant X1, mRNA.
ACCESSION   XM_003748970
VERSION     XM_003748970.5
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051336.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Jan 31, 2024 this sequence was replaced by
            NM_001427604.1.
            
            On Jan 21, 2021 this sequence version replaced XM_003748970.4.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4172
                     /gene="Scube2"
                     /note="signal peptide, CUB domain and EGF like domain
                     containing 2; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 4 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 17 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:499241"
                     /db_xref="RGD:1563998"
     CDS             528..3446
                     /gene="Scube2"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 2 isoform X1"
                     /protein_id="XP_003749018.1"
                     /db_xref="GeneID:499241"
                     /db_xref="RGD:1563998"
                     /translation="MGVAGCGRPSEARALLLLLLLLLPPPLPLLASAVPPDQGRTNGP
                     PEDVDECAQGLDDCHADALCQNTPTSYKCSCKPGYQGEGRQCEDMDECDNALNGGCVH
                     DCLNIPGNYRCTCFDGFMLAHDGHNCLDVDECLENNGGCQHTCTNVMGSYECRCKEGF
                     FLSDNQHTCIHRSEEGLSCMNKDHGCAHICKEAPRGSIACECRPGFELAKNQRDCILT
                     CNHGNGGCQHSCEDTAEGPECSCHPHYRLHADGRSCLEQEGTMLEGTESNATSVADGD
                     KRVKRRLLMETCAVNNGGCDRTCKDTSTGVHCSCPSGFTLQLDGKTCKDIDECQTRNG
                     GCNHFCKNTVGSFDCSCRKGFKLLTDEKSCQDVDECSLERTCDHSCINHPGTFTCACN
                     RGYTLYSFTHCGDTNECSVNNGGCQQVCVNTVGGYECQCHPGYKLHWNKKDCVEVKGV
                     LPTSTTPRVSLHCGKSGGGDRCFLRCHSGIHLSSGLQEAYSVTCGSPSLRSRQQKSND
                     SAFGDVVTVRTSVTFKLNEGKCSLQKAKLSPEGLRPALPERHSSVKESFHYANLTCSP
                     GKQVLGALGRPNAPKEMFITVEFERETYQKEVTASCNLSCVVKRTEKRLRKAVRTLKK
                     AAHREQFHLQLSGTDLDVAKKFPRVSGQQEETCGVGQGREESQCGLCQPGEYSADGFA
                     PCQPCALGTFQPDVGRTSCLPCGGGLPTKHLGATSFHDCETRVQCSPGHFYNTTTHRC
                     IRCPLGTYQPEFGKNNCVSCPGNTTTDFDGSTNITQCKNRKCGGELGDFTGYIESPNY
                     PGNYPANSECTWTINPPPKRRILIVVPEIFLPIEDDCGDYLVMRKTSSSNSVTTYETC
                     QTYERPIAFTSRSKKLWIQFKSNEGNSARGFQVPYVTYDEDYQELIEDIVRDGRLYAS
                     ENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESREMFPRSFIRLLRSKVSRFLRPYK
                     "
ORIGIN      
        1 ctctctgcct caaggtgcta ctttggggct aaggtatcag cgagtcctgg ctactgccag
       61 gctcaggaag caaaatgtgt gatggcctga gcaaagcagc tggggtcact cagatagctt
      121 tgtcctgagt tctcctgaca gggtatatgg aaccctaaga caatattgtt ggagccttgc
      181 tgggcctggt gtccagtgtc tcattgtgtg gggactaagc gagacactct ccgggcgccc
      241 tgtaactagt ttggaaggat cgggtcaagt tggagactca gcgagcgagc gatcgcgcag
      301 gattctgctg tcagccgctc gggccacggc ggccgcgccc cctccgcccc gggccgggga
      361 ggggccagac gggcggggcg gcacagagga ccgtgtgaaa atgaggccgg ggctcggggg
      421 cgggcggggc cgggggtgtc cgcgggcgca gaagccggga gcccgcacac gcctccccgc
      481 gccgctaccg ccgccggctc tccgccgctc cgcgcgctgc ggctgccatg ggggtcgcgg
      541 gctgcggtcg gccttcggag gcccgggcgc tgctgctgct gctactgctg ctgctgccgc
      601 cgccgctgcc gctgctggcc tctgccgtcc cacccgacca gggtcgcacc aacgggccgc
      661 ccgaggatgt ggatgagtgt gcccaagggc tggatgactg ccatgctgat gcactgtgtc
      721 aaaacacacc cacttcctac aagtgctctt gtaagcctgg ctaccaaggg gaaggcaggc
      781 agtgtgagga catggatgaa tgtgacaatg ctctcaatgg aggctgtgtc cacgactgtt
      841 taaacatccc aggcaactat cgctgcacct gttttgatgg cttcatgttg gctcatgacg
      901 gtcataattg tcttgatgtg gatgaatgct tggagaacaa tggcggctgc cagcacacct
      961 gcaccaacgt catggggagc tatgagtgtc gctgcaaaga aggtttcttt ctaagtgaca
     1021 atcagcacac ttgcattcac cgctcagaag agggcctgag ctgtatgaac aaggatcatg
     1081 gctgtgctca catctgcaaa gaggccccga gaggcagcat tgcctgtgaa tgcaggcctg
     1141 gttttgaact ggccaaaaac cagagagact gcatcttgac ctgtaaccat ggaaatggtg
     1201 ggtgtcagca ctcctgtgaa gatactgcag aaggccccga gtgtagctgc catccacact
     1261 acaggttgca tgctgatggg agaagctgcc tagagcaaga gggtaccatg ctggagggga
     1321 cggagagcaa tgccacgtca gtggcagatg gagataaacg ggtgaagcgg cggctgctca
     1381 tggaaacgtg tgctgtcaac aatggaggct gcgaccgcac ctgtaaggac acgtcaacag
     1441 gcgtccactg cagttgtccc tctggattca ctctccagtt ggatgggaag acatgtaaag
     1501 atatcgatga gtgccagacc cggaatggag gttgtaatca tttctgcaaa aacactgtgg
     1561 gcagttttga ctgcagctgc agaaaaggat ttaaattact aacagacgag aagtcttgcc
     1621 aagatgtgga tgaatgttct ttggaaagga cctgtgacca cagctgcatc aaccaccctg
     1681 gtacatttac gtgtgcctgc aatagaggat acactctcta tagcttcacc cactgtggag
     1741 acaccaacga gtgcagcgtc aacaacggag gctgccagca agtttgcgtc aacacagtgg
     1801 gtggctatga gtgccagtgt catccagggt acaagctcca ctggaataaa aaagactgtg
     1861 tggaagtaaa gggcgtcctg cccactagca cgacaccccg tgtgtccctg cactgtggta
     1921 aaagtggtgg aggagacagg tgcttcctaa gatgccattc tggcattcac ctctcgtcag
     1981 gactgcaaga ggcttactct gtcacctgcg gctctccctc tctcagaagc agacagcaaa
     2041 aatcaaatga ctctgctttt ggggatgtcg tcaccgtcag gacaagtgta acctttaagc
     2101 taaacgaagg caagtgtagt ttgcaaaagg ccaagctgtc tcccgagggc ctgcgaccag
     2161 cattaccaga gaggcacagc tcagtaaaag agagcttcca ctacgcaaac cttacatgca
     2221 gccctggcaa gcaagtcctg ggagcccttg gccgaccgaa cgcccctaag gaaatgttta
     2281 tcacggttga gtttgaacgg gaaacttacc aaaaggaggt gacagcttcc tgcaatctga
     2341 gctgcgtggt gaagcgaaca gagaagaggc tgcggaaagc tgtgcgcaca ctcaagaagg
     2401 ctgcccacag ggaacagttt cacctgcagc tctcaggcac ggacctcgat gtggctaaaa
     2461 agtttccccg agtgtcggga cagcaggagg agacctgtgg agtgggccag ggtcgtgaag
     2521 aaagccagtg tggcctttgt cagccgggtg aatattcagc agatggattt gccccttgcc
     2581 agccctgtgc cctgggcacc tttcagcctg atgtgggccg tacatcttgc ctcccgtgtg
     2641 gaggaggtct tcctactaag catctgggag ccacttcctt ccatgattgt gaaaccagag
     2701 ttcaatgttc gcctgggcat ttctacaaca ccaccacaca ccgatgtatc cgttgcccac
     2761 tgggaacata ccagcctgaa tttggaaaaa ataattgtgt ttcctgcccg ggaaatacta
     2821 ccaccgactt tgacggctcc accaacataa cacagtgtaa aaacagaaag tgtggagggg
     2881 agctggggga tttcacaggg tacattgaat ccccaaacta ccctgggaac tacccagcca
     2941 actcagagtg cacctggacc attaacccac cccccaaacg ccggatcctg attgtggtcc
     3001 ctgagatctt cctgcccata gaggatgact gcggtgacta cctggtgatg cggaaaacct
     3061 cttcatcaaa ttctgtgacc acatatgaaa cctgccagac ctatgaacgc cccatcgcct
     3121 tcacctccag atcaaagaag ctgtggattc agttcaagtc caatgaaggg aacagtgccc
     3181 gaggattcca ggttccctat gtgacatacg atgaagacta ccaggagctc attgaagaca
     3241 tagtccgaga tggcaggctc tatgcgtctg agaaccacca ggaaatactg aaggataaga
     3301 aactgatcaa ggctctgttt gatgtcctgg cacatcccca gaactatttc aagtacacag
     3361 cccaggagtc ccgggagatg tttccaagat ctttcatccg attgctacgt tccaaagtgt
     3421 caaggtttct gaggccttac aaatgactct tcccacatac caccctgaac agggtcctac
     3481 tgtgggctca gtgggctgca gccgtctccc ctccacacgc cggcctgcca tcggatcatt
     3541 gccacgccca gtatcagtga ctggtcagag ttcagttttt atagacagca cagatatctt
     3601 ggtaaactga acttgggttt tctctcccag tatttcagat gtggactgca tacatcttga
     3661 gcagcactgt gtgggcgcat gccctggatg tcctgagggc tggctggact ggcctcccct
     3721 tgggttctca ctccttcaac tgtctgtagt ggaaaggagg ccactctagc tgcatctcca
     3781 tttcctcctg gccggtcctc tcccagggag catctgtact catcagagtg accaggccaa
     3841 agaggcaagc aggaggggag gacaagcact ccaggagact cccgggcctt ctgagtcagg
     3901 ggaagactca ggtcctcccg ggcttctgca gcctctgcaa tgccagttta atcctaaccg
     3961 agtgggtcct aagcagtgat ttttttttta aagaccagaa agaattagaa ataaaaaggc
     4021 ccccctggag gcatgaatag cacgcacatg cacatgcaca cgtgtacaca cacacacaca
     4081 cacacacaca cacacacaca cacacacaca taccacacca cacacacacc acaccacaca
     4141 cacacacaca caccacacac acacacacac tc
//
