LOCUS       NM_017046               2966 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Rattus norvegicus sodium channel epithelial 1 subunit gamma
            (Scnn1g), mRNA.
ACCESSION   NM_017046
VERSION     NM_017046.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Yin,L.P., Zhu,H., Zhu,R.Y. and Huang,L.
  TITLE     A novel SCNN1G mutation in a PHA I infant patient correlates with
            nephropathy
  JOURNAL   Biochem Biophys Res Commun 519 (2), 415-421 (2019)
   PUBMED   31522814
  REMARK    GeneRIF: The SCNN1G defects triggered programmed cell death via
            inhibiting miR-21 and upregulating PTEN, which then orchestrated
            the key downstream regulators, including Bcl2, Bax2, and cleaved
            Caspse-3 in a way that favors cell apoptosis.
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Sharma,S. and Hanukoglu,I.
  TITLE     Mapping the sites of localization of epithelial sodium channel
            (ENaC) and CFTR in segments of the mammalian epididymis
  JOURNAL   J Mol Histol 50 (2), 141-154 (2019)
   PUBMED   30659401
  REMARK    GeneRIF: Based on the widespread presence of ENaC along the
            epididymis we suggest that mutations in ENaC subunits may also be
            associated with male infertility.
REFERENCE   3  (bases 1 to 2966)
  AUTHORS   Garcia-Caballero,A., Gandini,M.A., Huang,S., Chen,L., Souza,I.A.,
            Dang,Y.L., Stutts,M.J. and Zamponi,G.W.
  TITLE     Cav3.2 calcium channel interactions with the epithelial sodium
            channel ENaC
  JOURNAL   Mol Brain 12 (1), 12 (2019)
   PUBMED   30736831
  REMARK    GeneRIF: these findings reveal ENaC as an interactor and potential
            regulator of Cav3.2 calcium channels expressed in neuronal tissues.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2966)
  AUTHORS   Zachar,R., Al-Mashhadi,A., Dimke,H., Svenningsen,P., Jensen,B.L.
            and Carlstrom,M.
  TITLE     Hydronephrosis is associated with elevated plasmin in urine in
            pediatric patients and rats and changes in NCC and gamma-ENaC
            abundance in rat kidney
  JOURNAL   Am J Physiol Renal Physiol 315 (3), F547-F557 (2018)
   PUBMED   29767556
  REMARK    GeneRIF: The presence of active plasmin in urine in hydronephrosis
            could cause abnormal proteolytic activation of ENaC-gamma and
            impaired ability to excrete Na via NCC.
REFERENCE   5  (bases 1 to 2966)
  AUTHORS   Loh,S.Y., Giribabu,N. and Salleh,N.
  TITLE     Changes in plasma aldosterone and electrolytes levels, kidney
            epithelial sodium channel (ENaC) and blood pressure in normotensive
            WKY and hypertensive SHR rats following gonadectomy and chronic
            testosterone treatment
  JOURNAL   Steroids 128, 128-135 (2017)
   PUBMED   28954214
  REMARK    GeneRIF: Decreased in plasma aldosterone, Na(+) and ENaC levels in
            kidneys under testosterone influence indicated that
            testosterone-induced increased in mean arterial pressure (MAP) were
            not due to increased plasma aldosterone and ENaC levels in kidneys,
            and thus the testosterone effect on MAP likely involve other
            mechanisms.
REFERENCE   6  (bases 1 to 2966)
  AUTHORS   Strautnieks,S.S., Thompson,R.J., Gardiner,R.M. and Chung,E.
  TITLE     A novel splice-site mutation in the gamma subunit of the epithelial
            sodium channel gene in three pseudohypoaldosteronism type 1
            families
  JOURNAL   Nat Genet 13 (2), 248-250 (1996)
   PUBMED   8640238
REFERENCE   7  (bases 1 to 2966)
  AUTHORS   Staub,O., Dho,S., Henry,P., Correa,J., Ishikawa,T., McGlade,J. and
            Rotin,D.
  TITLE     WW domains of Nedd4 bind to the proline-rich PY motifs in the
            epithelial Na+ channel deleted in Liddle's syndrome
  JOURNAL   EMBO J 15 (10), 2371-2380 (1996)
   PUBMED   8665844
REFERENCE   8  (bases 1 to 2966)
  AUTHORS   Hansson,J.H., Nelson-Williams,C., Suzuki,H., Schild,L.,
            Shimkets,R., Lu,Y., Canessa,C., Iwasaki,T., Rossier,B. and
            Lifton,R.P.
  TITLE     Hypertension caused by a truncated epithelial sodium channel gamma
            subunit: genetic heterogeneity of Liddle syndrome
  JOURNAL   Nat Genet 11 (1), 76-82 (1995)
   PUBMED   7550319
REFERENCE   9  (bases 1 to 2966)
  AUTHORS   Lingueglia,E., Renard,S., Waldmann,R., Voilley,N., Champigny,G.,
            Plass,H., Lazdunski,M. and Barbry,P.
  TITLE     Different homologous subunits of the amiloride-sensitive Na+
            channel are differently regulated by aldosterone
  JOURNAL   J Biol Chem 269 (19), 13736-13739 (1994)
   PUBMED   8188647
REFERENCE   10 (bases 1 to 2966)
  AUTHORS   Canessa,C.M., Schild,L., Buell,G., Thorens,B., Gautschi,I.,
            Horisberger,J.D. and Rossier,B.C.
  TITLE     Amiloride-sensitive epithelial Na+ channel is made of three
            homologous subunits
  JOURNAL   Nature 367 (6462), 463-467 (1994)
   PUBMED   8107805
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Sep 13, 2018 this sequence version replaced NM_017046.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X77933.1, X78034.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16418971, SAMN16418986
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                JAXUCZ010000001.1  185736225-185736273
            50-398              JAXUCZ010000001.1  185739611-185739959
            399-702             JAXUCZ010000001.1  185741830-185742133
            703-893             JAXUCZ010000001.1  185743512-185743702
            894-997             JAXUCZ010000001.1  185744882-185744985
            998-1161            JAXUCZ010000001.1  185747797-185747960
            1162-1260           JAXUCZ010000001.1  185762147-185762245
            1261-1378           JAXUCZ010000001.1  185764267-185764384
            1379-1457           JAXUCZ010000001.1  185765166-185765244
            1458-1515           JAXUCZ010000001.1  185765327-185765384
            1516-1577           JAXUCZ010000001.1  185765572-185765633
            1578-1653           JAXUCZ010000001.1  185768377-185768452
            1654-2966           JAXUCZ010000001.1  185768787-185770099
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q36"
     gene            1..2966
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /note="sodium channel epithelial 1 subunit gamma"
                     /db_xref="GeneID:24768"
                     /db_xref="RGD:3641"
     exon            1..49
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            50..398
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..2034
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /note="amiloride-sensitive sodium channel subunit gamma;
                     amiloride sensitive sodium channel gamma1 subunit; SCNEG;
                     gamma-ENaC; gamma-NaCH; pithelial Na(+) channel subunit
                     gamma; epithelial Na(+) channel subunit gamma;
                     nonvoltage-gated sodium channel 1 subunit gamma; Sodium
                     channel, nonvoltage-gated 1, gamma (epithelial); sodium
                     channel, nonvoltage-gated, type I, gamma; epithelial
                     sodium channel gamma subunit; sodium channel,
                     non-voltage-gated 1, gamma subunit; sodium channel
                     epithelial 1 gamma subunit"
                     /codon_start=1
                     /product="epithelial sodium channel subunit gamma"
                     /protein_id="NP_058742.2"
                     /db_xref="GeneID:24768"
                     /db_xref="RGD:3641"
                     /translation="MAPGEKIKAKIKKNLPVRGPQAPTIKDLMHWYCMNTNTHGCRRI
                     VVSRGRLRRLLWIAFTLTAVALIIWQCALLVFSFYTVSVSIKVHFQKLDFPAVTICNI
                     NPYKYSAVSDLLTDLDSETKQALLSLYGVKESRKRREAGSMPSTLEGTPPRFFKLIPL
                     LVFNENEKGKARDFFTGRKRKISGKIIHKASNVMHVHESKKLVGFQLCSNDTSDCATY
                     TFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAKELLVTCFFDGMSCDARNFTLF
                     HHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYINEDEYNPFLVSSTGAKVLIHQ
                     QNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAAYSL
                     QICLYSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLYQAFVREELGC
                     QSVCKQSCSFKEWTLTTSLAQWPSEASEKWLLNVLTWDQSQQINKKLNKTDLAKLLIF
                     YKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQ
                     WHKAKDWWARRQTPPSTETPSSRQGQDNPALDTDDDLPTFTSAMRLPPAPGSTVPGTP
                     PPRYNTLRLDRAFSSQLTDTQLTNEL"
     exon            399..702
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            703..893
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            894..997
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1161
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1260
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1378
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1457
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1515
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1577
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1653
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..2966
                     /gene="Scnn1g"
                     /gene_synonym="ENaC"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgtctccag actgtacaca cgccagccgt gacccttcgg atctgagaga actctgcagg
       61 cagcaaagtc ctgtccttac catggcgcct ggagagaaga tcaaagccaa aatcaaaaag
      121 aatctgccgg ttcgaggccc ccaggcacca accattaagg acctgatgca ttggtactgc
      181 atgaacacca acacccacgg ctgccgccgc atcgtggtgt cccgaggccg ccttcggcgc
      241 ctgctgtgga tcgcgttcac gctaactgca gtggccctca ttatctggca gtgcgccctc
      301 ctcgtcttct ctttctacac cgtctctgtc tccatcaaag tccacttcca gaaactggat
      361 ttccctgccg tcactatctg caatatcaac ccttacaagt acagtgctgt gagtgacctt
      421 ctgactgact tggacagtga gacaaaacag gccttgctgt ccttgtatgg ggtcaaagaa
      481 tctcggaaac gccgggaagc aggatccatg ccgtccacct tggaaggcac accacccaga
      541 ttcttcaaac tgatcccact gctggtcttc aatgagaatg agaagggaaa ggccagggac
      601 ttcttcactg gccggaagcg gaaaatcagc gggaaaatca ttcacaaagc ttctaatgtc
      661 atgcacgttc atgagtcgaa gaaactggtg ggatttcaat tgtgctcaaa tgatacctct
      721 gactgcgcca cctacacctt cagctcagga atcaatgcca tccaggagtg gtacaagctt
      781 cactacatga atatcatggc acaggtgcct ctagagaaga aaatcaacat gagctattct
      841 gccaaagaac tgctggtgac ctgcttcttc gatgggatgt cctgtgatgc caggaacttc
      901 acgcttttcc accatccaat gtatgggaac tgctacacat ttaacaacaa agaaaatgcc
      961 accatcctca gcacctccat gggaggcagt gagtacgggc tgcaagtcat cttatacata
     1021 aacgaagatg aatacaaccc cttcctggtg tcctccactg gagccaaggt gcttatccat
     1081 cagcagaatg aatacccctt catcgaagac gtggggatgg agatcgagac agcaatgtcc
     1141 acttccatag gaatgcacct gacagaatcc ttcaagctga gcgaacctta cagccagtgc
     1201 acagaggatg gcagtgatgt gcccgtcaca aacatctaca acgctgccta ctccctccag
     1261 atctgccttt attcatgctt ccaaacaaag atggtggaaa aatgtggctg tgcccagtac
     1321 agccagcctc tgcctccagc agccaattac tgcaactacc agcaacaccc caactggatg
     1381 tattgctact accagttgta ccaggccttt gtccgggaag aactgggctg ccaatcagtg
     1441 tgcaagcaat cctgtagctt taaggaatgg acactgacca ccagcttggc acaatggccg
     1501 tctgaggctt ccgagaaatg gttgctgaat gttctcacct gggaccaaag ccagcaaata
     1561 aacaaaaagc tcaacaagac tgacctggcc aagctcttga tattctacaa agacctgaac
     1621 caaagatcca tcatggagag cccagccaac agtattgaga tgctcctgtc caactttggt
     1681 ggacagctcg gcctgtggat gagctgctca gtcgtctgtg tcattgaaat cattgaagtc
     1741 ttcttcattg acttcttctc tatcatcgcc cgccgtcagt ggcacaaagc caaggattgg
     1801 tgggcccgta ggcagacacc gccctccact gagactccct ccagccggca aggccaggat
     1861 aatccagctc tggatacgga cgatgacctg cccactttta cctctgctat gcgcctgcct
     1921 ccagccccgg ggtccacggt gcctggcaca ccacctccca gatacaatac cttgcgcttg
     1981 gatagagcct tttcatccca gctcacagac actcagttga ccaatgagtt gtaagacagg
     2041 attgggaaac aatcatggtt tgaggaggct ctcttcctga cacagatggc cctcctatcc
     2101 ttgctctgtc tttcaataca tgactaacag cctgctgtga accggatagg gcatgagaat
     2161 ccacagaaga acctgatggg acctgcccca acactcataa cttccagaat aagctatatg
     2221 ctacaacctg gacttggcac ttatgagcta aagagctagc agtaatggcc tgggccaaca
     2281 gtgggctctt cacaggatcc tgagagagaa tcatgcttca gagccccaaa ctcaggagtt
     2341 tacccacgct aaccctgact tagcctgtgg ctcaagcaca tgatcttggg tagcaaggcc
     2401 agccagctat tgcctgacat gtgtgataga agaaattagc accatccttg gatcctaaca
     2461 gttggttgct gacctcaacc attgacattg ggacaaggtg ttctctgtgg cctcagacag
     2521 acagttggcc cagagcctgg ggatcatgtt tggagagggc acagtagaga gaagccaagg
     2581 ggtgtggtag gatgtcccca ctctgagagc agggtggggc agtagagaag agccagttaa
     2641 agggcatagc tggacactcc tgccaaatgg tattcccaca atctcagctc aagtggtata
     2701 ctttgagttg taccaagcac ttacataagt tactctaggg cataacaaac accagcctat
     2761 aggcccaatc ctttattttg tctttgaact gagagtatgc gtatgcgtga gagtgtgtgt
     2821 atgaatgtgt gtatatgtct gtatgtgttt ataattttta tatagctaat atatacattt
     2881 atcaaaaaga taatattttg tgacttgtga aaattatatg agattcaaat gttactgttt
     2941 ataaataaag ttttgtggat aggcaa
//
