LOCUS       NM_012648               2445 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Rattus norvegicus sodium channel epithelial 1 subunit beta
            (Scnn1b), mRNA.
ACCESSION   NM_012648
VERSION     NM_012648.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Rauh,R., Hoerner,C. and Korbmacher,C.
  TITLE     deltabetagamma-ENaC is inhibited by CFTR but stimulated by cAMP in
            Xenopus laevis oocytes
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 312 (2), L277-L287 (2017)
   PUBMED   27941075
  REMARK    GeneRIF: deltabetagamma-ENaC is inhibited by CFTR but activated by
            cyclic AMP.
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Li,Y.L., Zhang,D., Tu,H. and Muelleman,R.L.
  TITLE     Altered ENaC is Associated With Aortic Baroreceptor Dysfunction in
            Chronic Heart Failure
  JOURNAL   Am J Hypertens 29 (5), 582-589 (2016)
   PUBMED   26297031
  REMARK    GeneRIF: Decreased expression of Scnn1b/g and activation of ENaC
            could be involved in attenuation of the aortic baroreceptor
            sensitivity in congestive heart failure.
REFERENCE   3  (bases 1 to 2445)
  AUTHORS   Abramova,T.O., Smolenskaya,S.E., Antonov,E.V., Redina,O.E. and
            Markel,A.L.
  TITLE     [Expression of Catechol-O-Methyltransferase (Comt),
            Mineralocorticoid Receptor (Mlr), and Epithelial Sodium Channel
            (ENaC) Genes in Kidneys of Hypertensive ISIAH Rats at Rest and
            during Response to Stress]
  JOURNAL   Genetika 52 (2), 206-214 (2016)
   PUBMED   27215035
  REMARK    GeneRIF: The discovered changes in the expression level of
            catechol-o-methyltransferase , mineralocorticoid receptor , and
            b-subunit of epithelial sodium channel confirm their involvement in
            increased sympathetic stimulation of the of hypertensive rats
REFERENCE   4  (bases 1 to 2445)
  AUTHORS   Warrington,J.P., Coleman,K., Skaggs,C., Hosick,P.A., George,E.M.,
            Stec,D.E., Ryan,M.J., Granger,J.P. and Drummond,H.A.
  TITLE     Heme oxygenase-1 promotes migration and beta-epithelial Na+ channel
            expression in cytotrophoblasts and ischemic placentas
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 306 (9), R641-R646 (2014)
   PUBMED   24553299
  REMARK    GeneRIF: Suggest that beta-ENaC mediates cytotrophoblast migration
            and increasing beta-ENaC expression by HO-1 induction enhances
            migration.
REFERENCE   5  (bases 1 to 2445)
  AUTHORS   Warnock,D.G., Kusche-Vihrog,K., Tarjus,A., Sheng,S.,
            Oberleithner,H., Kleyman,T.R. and Jaisser,F.
  TITLE     Blood pressure and amiloride-sensitive sodium channels in vascular
            and renal cells
  JOURNAL   Nat Rev Nephrol 10 (3), 146-157 (2014)
   PUBMED   24419567
  REMARK    Review article
REFERENCE   6  (bases 1 to 2445)
  AUTHORS   Kreutz,R., Struk,B., Rubattu,S., Hubner,N., Szpirer,J., Szpirer,C.,
            Ganten,D. and Lindpaintner,K.
  TITLE     Role of the alpha-, beta-, and gamma-subunits of epithelial sodium
            channel in a model of polygenic hypertension
  JOURNAL   Hypertension 29 (1 Pt 1), 131-136 (1997)
   PUBMED   9039092
REFERENCE   7  (bases 1 to 2445)
  AUTHORS   Staub,O., Dho,S., Henry,P., Correa,J., Ishikawa,T., McGlade,J. and
            Rotin,D.
  TITLE     WW domains of Nedd4 bind to the proline-rich PY motifs in the
            epithelial Na+ channel deleted in Liddle's syndrome
  JOURNAL   EMBO J 15 (10), 2371-2380 (1996)
   PUBMED   8665844
REFERENCE   8  (bases 1 to 2445)
  AUTHORS   Chang,S.S., Grunder,S., Hanukoglu,A., Rosler,A., Mathew,P.M.,
            Hanukoglu,I., Schild,L., Lu,Y., Shimkets,R.A., Nelson-Williams,C.,
            Rossier,B.C. and Lifton,R.P.
  TITLE     Mutations in subunits of the epithelial sodium channel cause salt
            wasting with hyperkalaemic acidosis, pseudohypoaldosteronism type 1
  JOURNAL   Nat Genet 12 (3), 248-253 (1996)
   PUBMED   8589714
REFERENCE   9  (bases 1 to 2445)
  AUTHORS   Shimkets,R.A., Warnock,D.G., Bositis,C.M., Nelson-Williams,C.,
            Hansson,J.H., Schambelan,M., Gill,J.R. Jr., Ulick,S., Milora,R.V.,
            Findling,J.W. et al.
  TITLE     Liddle's syndrome: heritable human hypertension caused by mutations
            in the beta subunit of the epithelial sodium channel
  JOURNAL   Cell 79 (3), 407-414 (1994)
   PUBMED   7954808
REFERENCE   10 (bases 1 to 2445)
  AUTHORS   Canessa,C.M., Schild,L., Buell,G., Thorens,B., Gautschi,I.,
            Horisberger,J.D. and Rossier,B.C.
  TITLE     Amiloride-sensitive epithelial Na+ channel is made of three
            homologous subunits
  JOURNAL   Nature 367 (6462), 463-467 (1994)
   PUBMED   8107805
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X77932.1.
            
            Sequence Note: This sequence has been modified as follows: removed
            17 bp suspected to be vector contamination from the 5' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X77932.1, U35174.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN09345238, SAMN11044071
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2445              X77932.1           18-2462
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q36"
     gene            1..2445
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /note="sodium channel epithelial 1 subunit beta"
                     /db_xref="GeneID:24767"
                     /db_xref="RGD:3640"
     exon            1..47
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            48..366
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..1972
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /note="amiloride-sensitive sodium channel subunit beta;
                     sodium channel, non-voltage-gated 1, beta subunit; SCNEB;
                     beta-ENaC; sodium channel epithelial 1 beta subunit;
                     beta-NaCH; epithelial Na(+) channel subunit beta;
                     nonvoltage-gated sodium channel 1 subunit beta; sodium
                     channel, nonvoltage-gated, type I, beta"
                     /codon_start=1
                     /product="epithelial sodium channel subunit beta"
                     /protein_id="NP_036780.1"
                     /db_xref="GeneID:24767"
                     /db_xref="RGD:3640"
                     /translation="MPVKKYLLKCLHRLQKGPGYTYKELLVWYCNNTNTHGPKRIICE
                     GPKKKAMWFLLTLLFACLVCWQWGVFIQTYLSWEVSVSLSMGFKTMNFPAVTVCNSSP
                     FQYSKVKHLLKDLYKLMEAVLDKILAPKSSHTNTTSTLNFTIWNHTPLVLIDERNPDH
                     PVVLNLFGDSHNSSNPAPGSTCNAQGCKVAMRLCSANGTVCTFRNFTSATQAVTEWYI
                     LQATNIFSQVLPQDLVGMGYAPDRIILACLFGTEPCSHRNFTPIFYPDYGNCYIFNWG
                     MTEKALPSANPGTEFGLKLILDIGQEDYVPFLASTAGARLMLHEQRTYPFIREEGIYA
                     MAGTETSIGVLLDKLQGKGEPYSPCTMNGSDVAIQNLYSDYNTTYSIQACLHSCFQDH
                     MIHNCSCGHYLYPLPAGEKYCNNRDFPDWAYCYLSLQMSVVQRETCLSMCKESCNDTQ
                     YKMTISMADWPSEASEDWILHVLSQERDQSSNITLSRKGIVKLNIYFQEFNYRTIEES
                     PANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEIIIDFIWITVIKLVASCKGLRRRRPQ
                     RPYTGPPPTVAELVEAHTNCVFQPDTTSCRPNAEVYPDQQTLPIPGTPPPNYDSLRLQ
                     PLDTMESDSEVEAI"
     exon            367..634
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            635..825
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            826..929
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1093
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1201
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1319
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1395
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1453
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1515
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1591
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..2430
                     /gene="Scnn1b"
                     /gene_synonym="RNENACB"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 accaccttag ctgccatcac tgcacattgg agcagctttc taaacaggtg ccaccatgcc
       61 agtgaagaag tacctgctga agtgcctgca caggctgcag aagggcccag gctacaccta
      121 caaggagctg ctagtgtggt actgcaacaa caccaacaca cacggcccca aacgcatcat
      181 ctgcgagggg cccaagaaga aggccatgtg gttcctgctc acgctgctct tcgcctgcct
      241 ggtgtgctgg cagtggggcg tcttcatcca gacctacctg agctgggagg tcagcgtctc
      301 gctctccatg ggcttcaaga ccatgaactt cccagcagtc accgtctgca attccagccc
      361 cttccagtac tccaaggtca agcacttgct gaaggacttg tacaagctga tggaggctgt
      421 cctggacaag attctggctc cgaagtccag ccacaccaac accaccagta ccctgaactt
      481 taccatctgg aaccacacgc ccctggtcct tattgatgag cggaaccctg accatccagt
      541 ggtcctcaac ttgtttgggg acagccacaa cagcagcaac ccagccccag gaagcacctg
      601 taatgcccaa gggtgcaaag tggccatgag gctgtgcagt gccaatggga ccgtgtgtac
      661 cttccgaaac ttcaccagtg ccacccaggc cgtgactgag tggtacatcc tgcaggccac
      721 caacatcttc tcacaagtgc tcccccagga cctggtgggg atgggctatg ctcctgatcg
      781 cataatccta gcctgtctgt ttggaacgga gccctgcagt catcggaact tcacacctat
      841 cttctaccct gattatggca actgctacat cttcaactgg ggcatgacag agaaggcact
      901 tccttctgcc aaccctggga ctgaatttgg tctcaagttg atcctggaca ttggtcagga
      961 ggactatgtc cccttccttg cgtccacagc aggggctagg ctgatgctcc acgagcagag
     1021 gacatacccc ttcattagag aagagggcat ctatgccatg gcaggaactg agacttctat
     1081 tggggtgctg ctggacaagc tgcagggcaa gggggagcca tacagtccct gcaccatgaa
     1141 cggctccgac gttgccattc agaacctcta cagtgactac aacacgacct attccatcca
     1201 ggcctgcctt cattcctgtt tccaagacca catgatccat aactgcagct gtggtcacta
     1261 cttgtaccct ttgcctgctg gggagaaata ctgcaacaac agagacttcc cagactgggc
     1321 ctactgctac ctaagcctac agatgagtgt ggtccagaga gagacctgcc tcagcatgtg
     1381 caaggagtcc tgcaacgaca cccagtataa gatgaccatc tccatggctg actggccatc
     1441 cgaggcctct gaggattgga tcctacatgt cctgtctcag gagcgggacc agagctcaaa
     1501 tatcaccctg agcaggaagg gtattgtcaa gctcaatatc tacttccaag agttcaacta
     1561 ccgtaccatc gaggaatcgc cggccaacaa tatcgtgtgg ctgctctcta acctgggtgg
     1621 ccagtttggc ttctggatgg ggggctcggt gctgtgcctc attgagtttg gggagatcat
     1681 tatcgacttc atttggatca ctgtcatcaa gctagtggcc tcctgtaaag gcctgcgcag
     1741 gaggcggcca cagcgaccct acactggccc gccgcccact gtggccgagc tggtggaggc
     1801 ccacaccaac tgtgtcttcc agcctgacac aaccagctgc aggcccaatg ccgaggtcta
     1861 ccctgaccaa cagactctgc ccattccggg cactccacct cccaactatg actccctgag
     1921 gctgcagccg ctggacacca tggagtctga cagcgaggtg gaggccatct agatccgcat
     1981 ccccacccgg gaactagtga actcaaaact gaggagtcac aaccattgtc agtgcctcat
     2041 ttcattagcc cttgtccaaa gagccagggc acagagccca tgtccctctg gtcagcccca
     2101 ggctgagggg ttcatagggt caagatgctg gtaccagaat attgaacttg tatccttttc
     2161 tagctcttgc ccaccctagc ccagtctttg ctctctgttg acctagcaga caggctccag
     2221 agacccatag agtccctctc ctggtgatag gccacttctc tgtcttgtta caacctcagt
     2281 ctcccagaat cagtgacctt gccctagttg gcattggctg aaccctgtta atagacttgg
     2341 gggtgtgcag accataggga gggagcatca ggtaagaagg cttgacaggg gagcacatgc
     2401 tttgttagaa aataaagaga gaaaacaccg aaaaaaaaaa aaaaa
//
