LOCUS       NM_031548               3395 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Rattus norvegicus sodium channel epithelial 1 subunit alpha
            (Scnn1a), mRNA.
ACCESSION   NM_031548
VERSION     NM_031548.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Zhang,X., Ge,Y., Bukhari,A.A., Zhu,Q., Shen,Y., Li,M., Sun,H.,
            Su,D. and Liang,X.
  TITLE     Estrogen negatively regulates the renal epithelial sodium channel
            (ENaC) by promoting Derlin-1 expression and AMPK activation
  JOURNAL   Exp Mol Med 51 (5), 1-12 (2019)
   PUBMED   31113930
  REMARK    GeneRIF: The results demonstrated that the loss of estrogen
            elevated systolic blood pressure in ovariectomized (OVX) rats. OVX
            rat kidneys showed increased alpha-ENaC expression but decreased
            derlin-1 expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Migneault,F., Gagnon,F., Pascariu,M., Laperle,J., Roy,A.,
            Dagenais,A. and Berthiaume,Y.
  TITLE     Post-Transcriptional Modulation of aENaC mRNA in Alveolar
            Epithelial Cells: Involvement of its 3' Untranslated Region
  JOURNAL   Cell Physiol Biochem 52 (5), 984-1002 (2019)
   PUBMED   30977984
  REMARK    GeneRIF: Taken together, these findings indicate that the alphaENaC
            3'UTR plays an important role in modulating transcript levels, and
            Dhx36 and Tial1 seem to be involved in posttranscriptional
            regulation of alphaENaC expression in alveolar epithelial cells.
REFERENCE   3  (bases 1 to 3395)
  AUTHORS   Sharma,S., Hanukoglu,A. and Hanukoglu,I.
  TITLE     Localization of epithelial sodium channel (ENaC) and CFTR in the
            germinal epithelium of the testis, Sertoli cells, and spermatozoa
  JOURNAL   J Mol Histol 49 (2), 195-208 (2018)
   PUBMED   29453757
REFERENCE   4  (bases 1 to 3395)
  AUTHORS   Hanukoglu,I., Boggula,V.R., Vaknine,H., Sharma,S., Kleyman,T. and
            Hanukoglu,A.
  TITLE     Expression of epithelial sodium channel (ENaC) and CFTR in the
            human epidermis and epidermal appendages
  JOURNAL   Histochem Cell Biol 147 (6), 733-748 (2017)
   PUBMED   28130590
REFERENCE   5  (bases 1 to 3395)
  AUTHORS   Zhang,J.L., Zhuo,X.J., Lin,J., Luo,L.C., Ying,W.Y., Xie,X.,
            Zhang,H.W., Yang,J.X., Li,D., Gao Smith,F. and Jin,S.W.
  TITLE     Maresin1 stimulates alveolar fluid clearance through the alveolar
            epithelial sodium channel Na,K-ATPase via the ALX/PI3K/Nedd4-2
            pathway
  JOURNAL   Lab Invest 97 (5), 543-554 (2017)
   PUBMED   28218740
  REMARK    GeneRIF: Studied the effect and underlining mechanisms of
            MaR1(maresin 1) in modulating alveolar fluid clearance on
            LPS-induced acute lung injury; found MaR1 improved epithelial
            sodium channel (ENaC) and Na,K-adenosine triphosphatase expression
            and activity.
REFERENCE   6  (bases 1 to 3395)
  AUTHORS   Waldmann,R., Champigny,G. and Lazdunski,M.
  TITLE     Functional degenerin-containing chimeras identify residues
            essential for amiloride-sensitive Na+ channel function
  JOURNAL   J Biol Chem 270 (20), 11735-11737 (1995)
   PUBMED   7744818
REFERENCE   7  (bases 1 to 3395)
  AUTHORS   Snyder,P.M., McDonald,F.J., Stokes,J.B. and Welsh,M.J.
  TITLE     Membrane topology of the amiloride-sensitive epithelial sodium
            channel
  JOURNAL   J Biol Chem 269 (39), 24379-24383 (1994)
   PUBMED   7929098
REFERENCE   8  (bases 1 to 3395)
  AUTHORS   Renard,S., Lingueglia,E., Voilley,N., Lazdunski,M. and Barbry,P.
  TITLE     Biochemical analysis of the membrane topology of the
            amiloride-sensitive Na+ channel
  JOURNAL   J Biol Chem 269 (17), 12981-12986 (1994)
   PUBMED   8175716
REFERENCE   9  (bases 1 to 3395)
  AUTHORS   Canessa,C.M., Schild,L., Buell,G., Thorens,B., Gautschi,I.,
            Horisberger,J.D. and Rossier,B.C.
  TITLE     Amiloride-sensitive epithelial Na+ channel is made of three
            homologous subunits
  JOURNAL   Nature 367 (6462), 463-467 (1994)
   PUBMED   8107805
REFERENCE   10 (bases 1 to 3395)
  AUTHORS   Canessa,C.M., Horisberger,J.D. and Rossier,B.C.
  TITLE     Epithelial sodium channel related to proteins involved in
            neurodegeneration
  JOURNAL   Nature 361 (6411), 467-470 (1993)
   PUBMED   8381523
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC070902.1.
            
            On May 24, 2004 this sequence version replaced NM_031548.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070902.1, U54699.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN09345330, SAMN11044071
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..3395
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /note="sodium channel epithelial 1 subunit alpha"
                     /db_xref="GeneID:25122"
                     /db_xref="RGD:3639"
     exon            1..859
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             366..2465
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /note="amiloride-sensitive sodium channel subunit alpha;
                     sodium channel epithelial 1 alpha subunit; epithelial
                     sodium channel alpha subunit; SCNEA; sodium channel,
                     non-voltage-gated 1 alpha subunit; alpha-ENaC; alpha-NaCH;
                     epithelial Na(+) channel subunit alpha; nonvoltage-gated
                     sodium channel 1 subunit alpha; sodium channel,
                     nonvoltage-gated 1 alpha; amiloride-sensitive epithelial
                     sodium channel alpha subunit; sodium channel,
                     nonvoltage-gated, type I, alpha"
                     /codon_start=1
                     /product="epithelial sodium channel subunit alpha"
                     /protein_id="NP_113736.1"
                     /db_xref="GeneID:25122"
                     /db_xref="RGD:3639"
                     /translation="MMLDHTRAPELNIDLDLHASNSPKGSMKGNQFKEQDPCPPQPMQ
                     GLGKGDKREEQGLGPEPSAPRQPTEEEEALIEFHRSYRELFQFFCNNTTIHGAIRLVC
                     SKHNRMKTAFWAVLWLCTFGMMYWQFALLFEEYLSYPVSLNINLNSDKLVFPAVTVCT
                     LNPYRYTEIKEELEELDRITEQTLFDLYKYNSSYTRQAGARRRSSRDLLGAFPHPLQR
                     LRTPPPPYSGRTARSGSSSVRDNNPQVDRKDWKIGFQLCNQNKSDCFYQTYSSGVDAV
                     REWYRFHYINILSRLSDTSPALEEEALGNFIFTCRFNQAPCNQANYSKFHHPMYGNCY
                     TFNDKNNSNLWMSSMPGVNNGLSLTLRTEQNDFIPLLSTVTGARVMVHGQDEPAFMDD
                     GGFNLRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPSKYTQQVCIHSCFQ
                     ENMIKKCGCAYIFYPKPKGVEFCDYRKQSSWGYCYYKLQGAFSLDSLGCFSKCRKPCS
                     VINYKLSAGYSRWPSVKSQDWIFEMLSLQNNYTINNKRNGVAKLNIFFKELNYKTNSE
                     SPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIFDLLVITLLMLLRRFRSRYWSPG
                     RGARGAREVASTPASSFPSRFCPHPTSPPPSLPQQGMTPPLALTAPPPAYATLGPSAP
                     PLDSAAPDCSACALAAL"
     exon            860..1133
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1324
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1428
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1592
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1691
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1809
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1888
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..1946
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2002
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2078
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..3375
                     /gene="Scnn1a"
                     /gene_synonym="ENaCA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgaggcagg atcagagagc ctggcacaga aagggagacc caaagagaag cgggagtcag
       61 ctgggccaag agggcgtgaa agctggagcc agtcaaacag tccgggagga aaaaagggca
      121 agagggagag acgctaagcc aggcagtgcc tgctgtgggg acccagggag gcgctagcgg
      181 gcaaacgaag gtggccttcg ctgtgaagtc cagtggccac tccagagaag ctcaatactg
      241 cttggttggc cccgactcca gaaggtcagc tggctcctgg aaaggtggag gagggtggga
      301 gggagagtga actcagcctg ggatgcgggc acggtcccgg acagccccat tctgccttca
      361 cgctaatgat gctggaccac accagagccc ctgagctcaa cattgaccta gaccttcacg
      421 cctccaactc gcctaagggg tccatgaagg gcaaccaatt caaggagcaa gacccttgtc
      481 ctcctcagcc catgcaagga ctggggaagg gggacaaacg tgaagagcag ggcctgggcc
      541 cggaaccctc agcaccccgg cagcccaccg aggaggagga ggcactgatt gagttccacc
      601 gctcctaccg ggagctcttc cagttcttct gcaacaacac caccatccac ggggccatcc
      661 gcctggtgtg ctccaaacac aaccgcatga agacggcctt ctgggcggtg ctgtggctgt
      721 gcaccttcgg catgatgtac tggcagttcg ccttgctgtt cgaggagtac ctcagctacc
      781 cagtgagcct caacatcaac ctcaattcag acaagctggt cttccctgcc gtcactgtct
      841 gcacccttaa tccttacaga tacactgaaa ttaaagagga gctggaagag ctggaccgca
      901 tcacggagca gacgcttttt gacttgtaca aatacaactc ttcctacact cgccaggctg
      961 gggcccgacg ccgcagctcc cgcgacctcc tgggtgcttt cccgcacccc ctgcagcgcc
     1021 tgcgcactcc acctccgccc tactccggcc gcacggcgcg cagcgggtct tccagcgtac
     1081 gcgacaacaa tccccaagtg gaccggaagg actggaagat cggcttccaa ctgtgcaacc
     1141 agaacaaatc agactgtttc taccagacat actcctctgg ggtggatgca gtgagggagt
     1201 ggtaccgctt ccattacatc aacattctgt ccaggctgtc ggacacctcg cccgctctag
     1261 aggaagaagc cctgggcaac ttcatcttca cctgtcgctt caaccaggcc ccctgcaacc
     1321 aggcgaatta ttccaagttc caccacccca tgtacgggaa ctgctacact ttcaatgaca
     1381 agaacaactc caatctctgg atgtcctcca tgcctggagt caacaatggt ttgtccctga
     1441 cactgcgcac agagcagaat gacttcatcc ccctgctgtc cacagtgacg ggggccaggg
     1501 tgatggtgca tggtcaggat gagcctgcct ttatggatga tggtggcttc aacttgaggc
     1561 ctggcgtgga gacctccatc agtatgagaa aggaagccct ggacagcctc ggaggaaatt
     1621 acggcgactg tactgagaat ggtagcgatg tcccggtcaa gaacctttac ccttccaagt
     1681 atacacagca ggtgtgcatt cactcctgct tccaggagaa catgatcaag aagtgtggct
     1741 gtgcctacat cttctaccct aagcccaagg gagttgagtt ctgtgactac cgaaagcaga
     1801 gctcctgggg ctattgctat tataaactgc agggcgcctt ctccttggac agcctgggct
     1861 gtttctccaa gtgtcggaag ccttgtagtg tgatcaacta caaactctct gccggctact
     1921 cacggtggcc atctgtgaag tcccaggatt ggatcttcga gatgctgtcc ttgcagaaca
     1981 attacactat taacaacaaa agaaacggag ttgcaaagct caacatcttc ttcaaggagc
     2041 tgaactataa aactaattcg gagtctcctt ctgtcacgat ggtcagcctc ctgtccaacc
     2101 tgggcagcca gtggagcctg tggtttggct cgtccgtgct ctctgtggtg gagatggcgg
     2161 agctcatctt cgacctcctg gtcatcacac ttctcatgct gctacgccgg ttccggagcc
     2221 ggtactggtc tccaggacga ggggccaggg gtgccaggga ggtggcctcc actccagctt
     2281 cctccttccc gtcccgtttc tgtcctcacc ctacatcccc accaccttct ttgccccagc
     2341 agggcatgac ccctcccctg gccctgacag cccctccacc tgcctatgct actctaggcc
     2401 ccagtgcccc tccactggac tctgcggcgc ctgactgttc tgcctgtgcc ctggcggcgc
     2461 tctgagagag gagaaggatc ctctcaccca gccctgagct cccttgtaaa ctgatggata
     2521 tctcacttca gcacatcttc cccagcggcc ccgccgagcc ctctgtggtg tgcctgcctg
     2581 agggaacagg ctgagtaagg ggcccaggaa gccgtccaga ggacaggggc taacgatctg
     2641 ctcagagctg ccctgcccct gcttctgaac actgccttcc acacaagcac agacaagttc
     2701 cctcttccct cggatcagcc cagccagact tggagctctg acaaggaact ttcctgggag
     2761 aggaccaagg aacaaataga acagccatcg cactcctacc cccgtgacca gaggctggcc
     2821 tggcctcact gccttcaggg acactgatgt ctgctcctct ccttgaactt gggtggggaa
     2881 ccccacccaa aagccccctt gttagttctt tggcaattcc cctcccctca ctcctcaggg
     2941 tggggactag agtaagacag accagggtag gcctggtgtt ctctctcatt ctcaagactc
     3001 tctcatttga tacaccatac cccagtgcct ctgtggtgtt gtctccttct ttctgtttcc
     3061 tcagcctgga agcttctttc actgtccctc agaacacaca gtctatgcat cccttagaaa
     3121 cctgcccaaa ggcaccattt cttttgtgaa ctcagttacc ctgttatgtc tcccccagaa
     3181 ttgtcgccct ctcccctcac ccccacagtg tactgtatta tatgttcaca ttcttttgtg
     3241 tctgcctccc tgggtaaact gagctccttg tgtggtcagg gatgagattt gctctgtttt
     3301 gtatccttcc ggtctagccc agtctcccac ttgggacggg taggcaggta ctcaataaag
     3361 gcttgttcca tcaaaaaaaa aaaaaaaaaa aaaaa
//
