LOCUS       NM_133289               9859 bp    mRNA    linear   ROD 13-DEC-2024
DEFINITION  Rattus norvegicus sodium voltage-gated channel alpha subunit 9
            (Scn9a), mRNA.
ACCESSION   NM_133289
VERSION     NM_133289.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Wang,C., Chen,R., Zhu,X., Zhang,X. and Lian,N.
  TITLE     Long noncoding RNA small nucleolar RNA host gene 5 facilitates
            neuropathic pain in spinal nerve injury by promoting SCN9A
            expression via CDK9
  JOURNAL   Hum Cell 37 (2), 451-464 (2024)
   PUBMED   38167752
  REMARK    GeneRIF: Long noncoding RNA small nucleolar RNA host gene 5
            facilitates neuropathic pain in spinal nerve injury by promoting
            SCN9A expression via CDK9.
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Yin,J.B., Liu,H.X., Dong,Q.Q., Wu,H.H., Liang,Z.W., Fu,J.T.,
            Zhao,W.J., Hu,H.Q., Guo,H.W., Zhang,T., Lu,Y.C., Jin,S., Wang,X.L.,
            Cao,B.Z., Wang,Z. and Ding,T.
  TITLE     Correlative increasing expressions of KIF5b and Nav1.7 in DRG
            neurons of rats under neuropathic pain conditions
  JOURNAL   Physiol Behav 263, 114115 (2023)
   PUBMED   36773735
  REMARK    GeneRIF: Correlative increasing expressions of KIF5b and Nav1.7 in
            DRG neurons of rats under neuropathic pain conditions.
REFERENCE   3  (bases 1 to 9859)
  AUTHORS   Hidaka,K., Maruta,T., Koshida,T., Kurogi,M., Kage,Y., Kouroki,S.,
            Shirasaka,T., Takeya,R. and Tsuneyoshi,I.
  TITLE     Extracellular signal-regulated kinase phosphorylation enhancement
            and NaV1.7 sodium channel upregulation in rat dorsal root ganglia
            neurons contribute to resiniferatoxin-induced neuropathic pain: The
            efficacy and mechanism of pulsed radiofrequency therapy
  JOURNAL   Mol Pain 18, 17448069221089784 (2022)
   PUBMED   35418262
  REMARK    GeneRIF: Extracellular signal-regulated kinase phosphorylation
            enhancement and NaV1.7 sodium channel upregulation in rat dorsal
            root ganglia neurons contribute to resiniferatoxin-induced
            neuropathic pain: The efficacy and mechanism of pulsed
            radiofrequency therapy.
REFERENCE   4  (bases 1 to 9859)
  AUTHORS   Liu,B.W., Zhang,J., Hong,Y.S., Li,N.B., Liu,Y., Zhang,M., Wu,W.Y.,
            Zheng,H., Lampert,A. and Zhang,X.W.
  TITLE     NGF-Induced Nav1.7 Upregulation Contributes to Chronic
            Post-surgical Pain by Activating SGK1-Dependent Nedd4-2
            Phosphorylation
  JOURNAL   Mol Neurobiol 58 (3), 964-982 (2021)
   PUBMED   33063281
  REMARK    GeneRIF: NGF-Induced Nav1.7 Upregulation Contributes to Chronic
            Post-surgical Pain by Activating SGK1-Dependent Nedd4-2
            Phosphorylation.
REFERENCE   5  (bases 1 to 9859)
  AUTHORS   Wang,G.J., Zhang,X., Huang,L.D. and Xiao,Y.
  TITLE     Involvement of the Sodium Channel Nav1.7 in Paclitaxel-induced
            Peripheral Neuropathy through ERK1/2 Signaling in Rats
  JOURNAL   Curr Neurovasc Res 17 (3), 267-274 (2020)
   PUBMED   32407275
  REMARK    GeneRIF: Involvement of the Sodium Channel Nav1.7 in
            Paclitaxel-induced Peripheral Neuropathy through ERK1/2 Signaling
            in Rats.
REFERENCE   6  (bases 1 to 9859)
  AUTHORS   Nassar,M.A., Stirling,L.C., Forlani,G., Baker,M.D., Matthews,E.A.,
            Dickenson,A.H. and Wood,J.N.
  TITLE     Nociceptor-specific gene deletion reveals a major role for Nav1.7
            (PN1) in acute and inflammatory pain
  JOURNAL   Proc Natl Acad Sci U S A 101 (34), 12706-12711 (2004)
   PUBMED   15314237
REFERENCE   7  (bases 1 to 9859)
  AUTHORS   Yang,Y., Wang,Y., Li,S., Xu,Z., Li,H., Ma,L., Fan,J., Bu,D.,
            Liu,B., Fan,Z., Wu,G., Jin,J., Ding,B., Zhu,X. and Shen,Y.
  TITLE     Mutations in SCN9A, encoding a sodium channel alpha subunit, in
            patients with primary erythermalgia
  JOURNAL   J Med Genet 41 (3), 171-174 (2004)
   PUBMED   14985375
REFERENCE   8  (bases 1 to 9859)
  AUTHORS   Sangameswaran,L., Fish,L.M., Koch,B.D., Rabert,D.K., Delgado,S.G.,
            Ilnicka,M., Jakeman,L.B., Novakovic,S., Wong,K., Sze,P.,
            Tzoumaka,E., Stewart,G.R., Herman,R.C., Chan,H., Eglen,R.M. and
            Hunter,J.C.
  TITLE     A novel tetrodotoxin-sensitive, voltage-gated sodium channel
            expressed in rat and human dorsal root ganglia
  JOURNAL   J Biol Chem 272 (23), 14805-14809 (1997)
   PUBMED   9169448
REFERENCE   9  (bases 1 to 9859)
  AUTHORS   Toledo-Aral,J.J., Moss,B.L., He,Z.J., Koszowski,A.G., Whisenand,T.,
            Levinson,S.R., Wolf,J.J., Silos-Santiago,I., Halegoua,S. and
            Mandel,G.
  TITLE     Identification of PN1, a predominant voltage-dependent sodium
            channel expressed principally in peripheral neurons
  JOURNAL   Proc Natl Acad Sci U S A 94 (4), 1527-1532 (1997)
   PUBMED   9037087
REFERENCE   10 (bases 1 to 9859)
  AUTHORS   Kozak,C.A. and Sangameswaran,L.
  TITLE     Genetic mapping of the peripheral sodium channel genes, Scn9a and
            Scn10a, in the mouse
  JOURNAL   Mamm Genome 7 (10), 787-788 (1996)
   PUBMED   8854872
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Dec 7, 2020 this sequence version replaced NM_133289.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF000368.1, U79568.1 [ECO:0000331]
            RNAseq introns       :: partial sample support SAMN14984043,
                                    SAMN17050745 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-510               JAXUCZ010000003.1  71700868-71701377   c
            511-818             JAXUCZ010000003.1  71631915-71632222   c
            819-937             JAXUCZ010000003.1  71629385-71629503   c
            938-1027            JAXUCZ010000003.1  71629009-71629098   c
            1028-1156           JAXUCZ010000003.1  71628378-71628506   c
            1157-1248           JAXUCZ010000003.1  71627004-71627095   c
            1249-1461           JAXUCZ010000003.1  71625816-71626028   c
            1462-1525           JAXUCZ010000003.1  71614807-71614870   c
            1526-1667           JAXUCZ010000003.1  71613540-71613681   c
            1668-1874           JAXUCZ010000003.1  71611614-71611820   c
            1875-2159           JAXUCZ010000003.1  71608319-71608603   c
            2160-2531           JAXUCZ010000003.1  71605780-71606151   c
            2532-2661           JAXUCZ010000003.1  71602936-71603065   c
            2662-2900           JAXUCZ010000003.1  71601778-71602016   c
            2901-3074           JAXUCZ010000003.1  71599900-71600073   c
            3075-3431           JAXUCZ010000003.1  71598848-71599204   c
            3432-3908           JAXUCZ010000003.1  71594471-71594947   c
            3909-4026           JAXUCZ010000003.1  71583348-71583465   c
            4027-4181           JAXUCZ010000003.1  71575484-71575638   c
            4182-4355           JAXUCZ010000003.1  71572468-71572641   c
            4356-4478           JAXUCZ010000003.1  71568902-71569024   c
            4479-4760           JAXUCZ010000003.1  71566766-71567047   c
            4761-4814           JAXUCZ010000003.1  71566016-71566069   c
            4815-4952           JAXUCZ010000003.1  71565088-71565225   c
            4953-5057           JAXUCZ010000003.1  71560720-71560824   c
            5058-5328           JAXUCZ010000003.1  71560190-71560460   c
            5329-9859           JAXUCZ010000003.1  71553189-71557719   c
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q21"
     gene            1..9859
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /note="sodium voltage-gated channel alpha subunit 9"
                     /db_xref="GeneID:78956"
                     /db_xref="RGD:69368"
     exon            1..510
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            511..818
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             561..6515
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /note="peripheral sodium channel 1; sodium channel protein
                     type IX subunit alpha; sodium channel, voltage-gated, type
                     IX, alpha subunit; voltage-gated sodium channel subunit
                     alpha Nav1.7; sodium channel, voltage-gated, type 9, alpha
                     polypeptide"
                     /codon_start=1
                     /product="sodium channel protein type 9 subunit alpha"
                     /protein_id="NP_579823.1"
                     /db_xref="GeneID:78956"
                     /db_xref="RGD:69368"
                     /translation="MAMLPPPGPQSFVHFTKQSLALIEQRISEEKAKEHKDEKKDDEE
                     EGPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKAIFRFNA
                     TPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTLSNPPEWTKNVEYTFT
                     GIYTFESLIKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRV
                     LRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCF
                     RKELEENETLESIMNTAESEEELKKYFYYLEGSKDALLCGFSTDSGQCPEGYICVKAG
                     RNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFY
                     LINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEFTSI
                     GRSRIMGLSESSSETSRLSSKSAKERRNRRKKKKQKMSSGEEKGDDEKLSKSGSEESI
                     RKKSFHLGVEGHHRTREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDLGSE
                     TEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCN
                     GVVSLVDGPSALMLPNGQLLPEVIIDKATSDDSGTTNQMRKKRLSSSYFLSEDMLNDP
                     HLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKLIYFIV
                     MDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLAVGNLIFTGIFAAEMVLKLIAMD
                     PYEYFQVGWNIFDSLIVTLSLIELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIK
                     IIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINVDCKLPRWHMNDFFH
                     SFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVYMMVMVIGNLVVLNLFLALLLSSFSS
                     DNLTAIEEDTDANNLQIAVARIKRGINYVKQTLREFILKSFSKKPKGSKDTKRTADPN
                     NKKENYISNRTLAEMSKDHNFLKEKDRISGYGSSLDKSFMDENDYQSFIHNPSLTVTV
                     PIAPGESDLEIMNTEELSSDSDSDYSKEKRNRSSSSECSTVDNPLPGEEEAEAEPVNA
                     DEPEACFTDGCVRRFPCCQVNVDSGKGKVWWTIRKTCYRIVEHSWFESFIVLMILLSS
                     GALAFEDIYIEKKKTIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLDFL
                     IVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIM
                     NVLLVCLIFWLIFSIMGVNLFAGKFYECVNTTDGSRFPTSQVANRSECFALMNVSGNV
                     RWKNLKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSVNVNEQPKYEYSLYMYIYFV
                     IFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKP
                     IPRPGNKFQGCIFDLVTNQAFDITIMVLICLNMVTMMVEKEGQTEYMDYVLHWINMVF
                     IILFTGECVLKLISLRHYYFTVGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVI
                     RLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVK
                     KEAGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPKKVHPGSSVEG
                     DCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVW
                     EKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFA
                     FTKRVLGEGGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEEVSATIIQRAYRR
                     YRLRQHVKNISSIYIKDGDRDDDLPNKEDTVFDNVNENSSPEKTDVTASTISPPSYDS
                     VTKPDQEKYETDKTEKEDKEKDESRK"
     exon            819..937
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1027
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1156
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1248
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1461
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1525
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1667
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1874
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..2159
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2531
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2661
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2900
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2901..3074
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3075..3431
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3432..3908
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3909..4026
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4027..4181
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4182..4355
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4478
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4479..4760
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4761..4814
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4815..4952
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4953..5057
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            5058..5328
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
     exon            5329..9859
                     /gene="Scn9a"
                     /gene_synonym="Nav1.7; PN1; Scn2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcacacaccc cacccagaga ggtggccgtg ggtgggaggc tgcgcgaccc acctcctgtc
       61 cctttttcta gcgcacagta cagaggctgc cgcatccccg gcatcaccgc gcccaatcgc
      121 caggtctccg ccctgcaagg tctacccggc tgggaggagg gagctagggg tggggacccg
      181 agagctcagg gagcatcctg gaggcctagc gacgcggctg gcagccgagg gcggggtcgc
      241 ctcatcctga gcagactgga aacagactcc gtgcaggcct cgcccgcgct ccagttgcga
      301 ctgtagggtt ttcattcctg cccactgcgc agactgggct gagctagcct gggtatccac
      361 gattcgcgac tcgtagtaac aggcactctg agcaacagga tttcagagaa agaagcagag
      421 gcaagaaaga agcctgggga gagaggaaga ctttccttgg atcagactcc gcaggtgcac
      481 acaccgggtg ggcatgatcc gtggggccag gcctcttagg taaggagtca aaggggaaat
      541 aaaacataca ggatgaaaag atggcgatgc tgcctcctcc aggacctcag agtttcgttc
      601 acttcacaaa acagtccctt gccctcattg aacagcgtat ttctgaagaa aaagccaagg
      661 aacacaaaga cgaaaagaaa gatgatgagg aagaaggccc caagcccagc agtgacttgg
      721 aagctgggaa acagctcccc ttcatctatg gagacattcc ccctggaatg gtgtcagagc
      781 ccctggagga cctggaccca tactatgctg acaaaaaaac ttttatagta ttgaacaaag
      841 ggaaagcaat cttccgtttc aacgccaccc ctgctttgta catgctgtct cccttcagtc
      901 ctctaagaag aatatctatt aagatcttag tgcactcctt attcagcatg ctaatcatgt
      961 gcacaattct gacgaactgc atattcatga ccttgagcaa ccctccagaa tggaccaaaa
     1021 atgtagagta cacttttact gggatatata cttttgaatc actcataaaa atccttgcaa
     1081 gaggcttttg cgtgggagaa ttcaccttcc tccgtgaccc ttggaactgg ctggactttg
     1141 ttgtcattgt ttttgcgtat ttaacagaat ttgtaaacct aggcaatgtt tcagctcttc
     1201 gaactttcag agtcttgaga gctttgaaaa ctatttctgt aatcccagga ctaaagacca
     1261 tcgtgggggc cctgatccag tcagtgaaga agctctctga cgtcatgatc ctcactgtgt
     1321 tctgtctcag tgtgtttgca ctaattggac tacagctgtt tatgggcaac ttgaagcata
     1381 aatgtttcag gaaggaactc gaagagaatg aaacattaga aagtatcatg aatactgctg
     1441 agagtgaaga agaattgaaa aaatattttt attacttgga gggatccaaa gatgctctac
     1501 tctgcggctt cagcacagat tcagggcagt gtccagaagg ctacatctgt gtgaaggctg
     1561 gcagaaaccc ggattatggc tacacgagct ttgacacatt cagctgggcc ttcttggcct
     1621 tgtttcggct aatgactcag gactactggg agaaccttta ccaacagact ctgcgtgctg
     1681 ctggcaaaac ctacatgatt ttctttgtcg tggttatttt tctgggctcc ttttacctga
     1741 taaacttgat cctggctgtg gtagccatgg cgtatgagga acagaaccag gccaacatcg
     1801 aagaagctaa acagaaagag ttagaatttc agcagatgtt agaccgactc aaaaaggagc
     1861 aggaagaagc tgaggcgatc gctgcagccg ctgctgagtt cacgagtata gggcggagca
     1921 ggatcatggg actctctgag agctcttcag aaacctccag gctgagctca aagagtgcca
     1981 aggagagaag aaaccgaaga aagaaaaaga aacagaagat gtccagtggc gaggaaaagg
     2041 gtgacgatga gaagctgtcc aagtcaggat cagaggaaag catccgaaag aaaagtttcc
     2101 atctcggtgt ggaagggcac caccggaccc gggaaaagag gctgtccacc cccaaccagt
     2161 cgccactcag cattcgtggg tccctgtttt ctgccaggcg cagcagcagg acgagtctgt
     2221 tcagttttaa ggggcgagga agagatctgg gatctgagac agaattcgct gatgatgagc
     2281 atagcatttt tggagacaac gagagcagaa ggggttcact attcgtaccc catagacccc
     2341 gggagcggcg cagcagtaac atcagtcagg ccagtaggtc cccgccagtg ctaccggtga
     2401 acgggaagat gcacagtgca gtggactgca atggcgtcgt gtcgcttgtt gatggaccct
     2461 cagccctcat gctccccaat ggacagcttc ttccagaggt gataatagat aaggcaactt
     2521 ccgacgacag cggcacgact aatcagatgc gcaaaaaaag gctctctagt tcttacttct
     2581 tgtctgagga catgctgaat gacccgcatc tcaggcaaag ggccatgagc agggcgagca
     2641 tactgaccaa cactgtggaa gaacttgaag aatctagaca aaaatgtcca ccatggtggt
     2701 acagatttgc tcacacattt ttaatctgga attgctctcc atattggata aaattcaaaa
     2761 agctcatcta ttttattgtg atggatcctt ttgtagatct tgcaattacc atttgcatag
     2821 ttttaaacac cttatttatg gctatggagc accacccaat gactgaagaa ttcaaaaatg
     2881 tccttgcagt ggggaacttg atctttacag ggatcttcgc agctgaaatg gtactgaagt
     2941 taatagccat ggacccctat gagtatttcc aagtagggtg gaatattttt gacagcctaa
     3001 ttgtgacgct gagtttgata gagcttttcc tagcagatgt ggaaggatta tcagttctgc
     3061 ggtcattcag attgctccga gtcttcaagt tggcaaagtc ctggcccaca ctgaacatgc
     3121 tcattaagat catcggcaac tcggtgggcg cactgggcaa cctgaccctg gtgctggcca
     3181 tcatcgtctt catttttgcc gtggtcggca tgcagctgtt tggaaagagc tacaaggagt
     3241 gtgtctgcaa gatcaatgtg gactgcaagc tgccgcgctg gcacatgaac gacttcttcc
     3301 actccttcct gatcgtgttc cgagtgctgt gtggggagtg gatagagacc atgtgggact
     3361 gcatggaggt cgcgggccag accatgtgcc ttattgttta catgatggtc atggtgattg
     3421 ggaaccttgt ggtcctgaac ctgtttctgg ctcttttgct gagttcattt agttctgaca
     3481 atcttacagc aattgaggaa gacaccgatg caaacaacct ccagatcgca gtggccagaa
     3541 ttaagagggg aatcaattat gtgaaacaga ccctgcgtga attcattcta aaatcatttt
     3601 ccaaaaagcc aaagggctcc aaggacacaa aacgaacagc agatcccaac aacaagaaag
     3661 aaaactatat ttcaaaccgt acccttgcgg agatgagcaa ggatcacaat ttcctcaaag
     3721 aaaaggatag gatcagtggt tatggcagca gtctagacaa aagctttatg gatgaaaatg
     3781 attaccagtc ctttatccat aaccccagcc tcacagtgac agtgccaatt gcacctgggg
     3841 agtctgattt ggagattatg aacacagaag agcttagcag tgactcagac agtgactaca
     3901 gcaaagagaa acggaaccga tcaagctctt ctgagtgcag cactgttgac aaccctctgc
     3961 caggagaaga ggaggctgaa gcagagcccg taaacgcaga tgagcctgaa gcctgcttta
     4021 cagatggttg tgtgaggaga tttccatgct gccaagttaa tgtagactct gggaaaggga
     4081 aagtttggtg gaccatcagg aagacgtgct acaggatagt tgaacacagc tggtttgaaa
     4141 gcttcatcgt tctcatgatc ctgctcagca gtggagctct ggcttttgaa gatatctata
     4201 ttgaaaagaa aaagaccatt aagattatcc tggagtatgc tgacaagata ttcacctaca
     4261 tcttcattct ggaaatgctt ctaaaatggg tcgcatatgg gtataaaaca tatttcacta
     4321 atgcctggtg ttggctggac ttcttaattg ttgatgtgtc tctagttact ttagtagcca
     4381 acactcttgg ctactcagac cttggcccca ttaaatctct acggacactg agggccctaa
     4441 gacccctaag agccttgtct agatttgaag gaatgagggt agtggtcaac gcactcatag
     4501 gagcaatccc ttccatcatg aacgtgcttc tcgtgtgcct tatattctgg ctaatattta
     4561 gcatcatggg agtcaatctg tttgctggca agttctatga gtgtgtcaac accaccgatg
     4621 ggtcacgatt tcctacatct caagttgcaa accgttctga gtgttttgcc ctgatgaacg
     4681 ttagtggaaa tgtgcgatgg aaaaacctga aagtaaactt cgacaacgtt gggcttggtt
     4741 acctgtcgct gcttcaagtt gcaacattca agggctggat ggatattatg tatgcagcag
     4801 ttgactctgt taatgtaaat gaacagccga aatacgaata cagtctctac atgtacattt
     4861 actttgtcat cttcatcatc ttcggctcat tcttcacgtt gaacctgttc attggtgtca
     4921 tcatagataa tttcaaccaa cagaaaaaaa agcttggagg tcaagatatc tttatgacag
     4981 aagaacagaa gaaatactat aatgcaatga agaagcttgg gtccaagaaa ccacaaaaac
     5041 caattccaag gccagggaac aaattccaag gatgtatatt tgacttagtg acaaaccaag
     5101 cttttgatat caccatcatg gttcttatat gcctcaacat ggtaaccatg atggtagaaa
     5161 aagaggggca aactgagtac atggattatg ttttacactg gatcaacatg gtcttcatta
     5221 tcctgttcac tggggagtgt gtgctgaagc taatctccct cagacattac tacttcactg
     5281 tgggttggaa catttttgat tttgtggtag tgatcctctc cattgtagga atgtttctcg
     5341 ctgagatgat agagaagtat ttcgtgtccc ctaccctgtt ccgagtcatc cgcctggcca
     5401 ggattggacg aatcctacgc ctgatcaaag gcgccaaggg gatccgcact ctgctctttg
     5461 ctctgatgat gtcccttcct gcgctgttca acatcggcct cctgcttttc ctggtcatgt
     5521 tcatctacgc catctttggg atgtccaact ttgcctacgt taaaaaggag gctggaatta
     5581 atgacatgtt caactttgag acttttggca acagcatgat ctgcttgttc caaatcacca
     5641 cctctgcggg ctgggacgga ctgctggccc ccatcctcaa cagcgcacct cctgattgtg
     5701 accctaaaaa agttcaccca ggaagttcag tggaagggga ctgtgggaac ccatccgtgg
     5761 ggattttcta ctttgtcagc tacatcatca tatccttcct ggtggtggtg aacatgtaca
     5821 tcgctgtcat cctggagaac ttcagtgttg ccaccgaaga gagcactgag cctctgagtg
     5881 aggacgactt tgagatgttc tacgaggtct gggagaagtt cgaccctgac gccactcagt
     5941 tcatagagtt ctgcaagctt tctgactttg cagctgccct ggatcctccc ctcctcatcg
     6001 caaagccaaa caaagtccag ctcattgcca tggacctgcc catggtgagt ggagaccgca
     6061 tccactgcct ggacatcttg tttgctttta caaagcgggt cctgggtgag ggtggagaga
     6121 tggattctct tcgttcacag atggaagaaa ggttcatgtc agccaatcct tctaaagtgt
     6181 cctatgaacc catcacgacc acactgaaga gaaaacaaga ggaggtgtcc gcgactatca
     6241 ttcagcgtgc ttacagacgg tatcgcctca gacaacacgt caagaatata tcgagtatat
     6301 acataaaaga tggagacagg gatgatgatt tgcccaataa agaagataca gtttttgata
     6361 acgtgaacga gaactcaagt ccggaaaaga cagatgtaac tgcctcaacc atctcgccac
     6421 cttcctatga cagtgtcaca aagccagatc aagagaaata tgaaacagac aaaacagaga
     6481 aggaagacaa agagaaagat gaaagcagga aatagagctt tggttttgat acactgttga
     6541 cagcctgtga aggttgactc actcgtgtta gtaagactct tttacggagg tctatgccaa
     6601 actcttttta tcaaaaaatt ctcaaaggca gcacagccat tagctctgat ccaacgaggc
     6661 agagggcagc atttacacat ggctatgttt tgctttggta aatgattctt ttaagatttg
     6721 agagtgtaac tctgaaggga agagccatca ctgcattcaa gggtgactta gacattctaa
     6781 aaatcagaaa accaagtcaa acattttctc atttgcctcc atgtcttttc tctaggttct
     6841 aaataaatgt gcacccatgt gaatctagaa atccaggcat acatgcacac atgcacacag
     6901 tcacaaatac aaacaggatt ggctacgatt tcaaattcta tgtaaatgtt tcactttggt
     6961 ttgcaatgaa atttccttgt aaaggaaatg ggattagtga cgaaggtttt gtcaaaacat
     7021 cttattatta gggaatcaga atctttaatc taaaaagaat tcagtttata tttttgaggt
     7081 gctgaaactt atcctacatt gcgtcaaaag caacttatag gaatctataa gatgtcatgg
     7141 aattgaaaaa cgtatgtggg ctacttattt aagaaatcac tttcatttgt atattagcat
     7201 cattcacctt gatttatgga catcttggac attttgtggt cacatgatgc tcccacaaaa
     7261 ttgtttagct gaaacttcag gcacacatca cagagaacag ctacccagtc ttacgctcca
     7321 ccctgttcat acaataacca cagaccttta aacattattt ggaatataaa tattaggttt
     7381 ctcaatataa acatgtgaat attaacagaa aagttaattt acagcgtttt acttactaat
     7441 gtctatccaa tctagtttgc acaatgagac agctctcacc tcttacagcc agttgccaca
     7501 tttctcttct tacgaagctt cggtttgtca gactttatct ctaagcaaat tctatgtttc
     7561 ctttttaatg aaacgtatcc tgaaaagcga ttagctactt gatactttag tgcttgtttg
     7621 agcagatgac tggagcatgg tatgtacccc atcttttaaa atacaatcct taatgtgttt
     7681 ggcagcttcc caagttacag ggaacgccgg gcttttaata tctctccgtt caccaggtgt
     7741 tttgtatgaa tgaaatgaca ttcaaagagt gagtctcatt ggcttgcttt attaccaacg
     7801 actttcctaa atgaggaact aatcctctga agcactatta tatccccttg taaataacat
     7861 agatgtcact tcgttcttaa tattctaatc catggtgtga catatatttt gcattgatca
     7921 gtaaagtagt ctgggataac cattttaaga gcatatacat tttgaggtag ttgaagttaa
     7981 tctaccaagg attagaaacc tactccaagt ttttaaagta cttgataaaa atgaatgacc
     8041 ctgacaaatt ctctcataat tttattccag caatgtgaaa tctttcctcc aacactttat
     8101 ccttgcagag aagaaagaaa aaacaaagat aagcccatgg gccatgtaaa caaaggggct
     8161 ttatctagaa gattctgaga gaacctttga agtaaatatg taaaacatac ttcctcagtt
     8221 tgcctttgaa attaaaaagt aacacaaaaa tatggattta ataagtcatc aaaacacatt
     8281 ctttatttct ggcccagttt aaaggaaagg attaaaaatc cctatagagg ggaagggaca
     8341 gagagagaaa gagggagaga agaaacaaag tactagggac aaaaagatta gacagaatac
     8401 gtccaggttt tcacactatg ggctttgtcc gggatgctgt ataaaagtct ctttccaaat
     8461 gtttgtcata aggggatcga agaaattaag tgcaagacta aaggttgttt acagatatca
     8521 ttaatcatta acagtttggg aagctgcttt tacatttcta tggaagatgc caaggaatgc
     8581 taagccagac aatacagatg cattgtgcat ctgtcaagcg gctaaaatat gtttgggcaa
     8641 cttaattagg gaaacacaca aagccttaca tttctgacaa ttttaataca tttaattaag
     8701 aaatcagtac ttttattatt tcacattcct aaaatataac tatatcgtcc atttttattg
     8761 atattatatg tgtggatgtt tgttgtagat tatctgtaat ttactattaa tgtttttaca
     8821 ctgatttttg tgaaaattta aaatatgttg gcaactggtt cctcaacaca atttttttag
     8881 cattacaaaa aataaacagg tataaaattt ctcttttttc tacatcatgt tgaacatatt
     8941 tcattctgat ttacacaatt ttaaatgtaa tattatttta tatgcatatg tttatctcca
     9001 aaatcactac ttatgacatc atatcaatta aaaatggtat ttgacactag gactgtattt
     9061 agtaaaagtt aaaagctttc accctttttc atgttgatta gggtttgcac taactataaa
     9121 atgtcaccca cggaagctac tagtcactga aattatttta cctattggtg gagaatggtg
     9181 actggccaga aaatatgagt tagcattaag tagaagacag catacgtgac cacaaatcca
     9241 tgcaggtagt ttgcgtgaaa acgggagaat ctcatttttc tgttttgtct cttctcactg
     9301 tgcaaaaagg acaagaaatc tttgggctat ttctaaattc gaattgtcac aatttgcaat
     9361 cataaatgtt tagcacgact ttgtagaatt ttgatagttt tataaaagag tgaaaaacaa
     9421 atgcatatgt aaataaagca gcccatacta acggattcat caaatgttaa tatgtaaata
     9481 aagcagccca tactagcaga ttcatcaaat gttaatatgt aaatacagca gcccatatga
     9541 gcagattcgt catatgctaa gggaattaat taaaaggaag caactaaata aggatggtca
     9601 gtgattgatc tgggtttaga acttgtgttt ggagccacca atttttaagt atgtattctc
     9661 actttattag ttttctaaag aaagcaatca agaattgctc ccagaaaatg agtaagtagc
     9721 catgaatata tgaatgctgt ttacaaaacc catagaccca tgaatgctca aaacgtctga
     9781 gtttgtaaaa aaaaatacat tgtagttata cttgacaatt taaaatgtta atagaatcta
     9841 aaataaaagt cgcttaaaa
//
