LOCUS       NM_019266               6548 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus sodium voltage-gated channel alpha subunit 8
            (Scn8a), mRNA.
ACCESSION   NM_019266
VERSION     NM_019266.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Liu,W., Zhang,R., Feng,H., Luo,J. and Zhu,H.
  TITLE     Increased expression of Nav1.6 of reactive astrocytes in the globus
            pallidus is closely associated with motor deficits in a model of
            Parkinson's disease
  JOURNAL   Glia 71 (12), 2850-2865 (2023)
   PUBMED   37572007
  REMARK    GeneRIF: Increased expression of Nav1.6 of reactive astrocytes in
            the globus pallidus is closely associated with motor deficits in a
            model of Parkinson's disease.
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Lin,M., Chen,X., Wu,S., Chen,P., Wan,H., Ma,S., Lin,N., Liao,Y.,
            Zheng,T., Jiang,J. and Zheng,X.
  TITLE     Upregulation of Nav1.6 Mediated by the p38 MAPK Pathway in the
            Dorsal Root Ganglia Contributes to Cancer-Induced Bone Pain in Rats
  JOURNAL   Cells 11 (21), 3375 (2022)
   PUBMED   36359772
  REMARK    GeneRIF: Upregulation of Nav1.6 Mediated by the p38 MAPK Pathway in
            the Dorsal Root Ganglia Contributes to Cancer-Induced Bone Pain in
            Rats.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6548)
  AUTHORS   Xie,W., Zhang,J., Strong,J.A. and Zhang,J.M.
  TITLE     Role of NaV1.6 and NaVbeta4 Sodium Channel Subunits in a Rat Model
            of Low Back Pain Induced by Compression of the Dorsal Root Ganglia
  JOURNAL   Neuroscience 402, 51-65 (2019)
   PUBMED   30699332
  REMARK    GeneRIF: Study found that knockdown of either NaV1.6 or NaVbeta4
            reduced pain behaviors and sensory neuron hyperexcitability in the
            chronic compression dorsal rook ganglia model of low back pain.
REFERENCE   4  (bases 1 to 6548)
  AUTHORS   Qin,S., Jiang,F., Zhou,Y., Zhou,G., Ye,P. and Ji,Y.
  TITLE     Local knockdown of Nav1.6 relieves pain behaviors induced by BmK I
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 49 (8), 713-721 (2017)
   PUBMED   28655185
  REMARK    GeneRIF: Data strongly suggest that voltage-gated sodium channel
            subunit alpha Nav1.6 ( Nav1.6) plays an indispensable role in the
            peripheral pain hypersensitivity induced by BmK I.
REFERENCE   5  (bases 1 to 6548)
  AUTHORS   Zhu,H., Zhao,Y., Wu,H., Jiang,N., Wang,Z., Lin,W., Jin,J. and Ji,Y.
  TITLE     Remarkable alterations of Nav1.6 in reactive astrogliosis during
            epileptogenesis
  JOURNAL   Sci Rep 6, 38108 (2016)
   PUBMED   27905510
  REMARK    GeneRIF: To clarify the exact expression patterns of Nav1.6 during
            epileptogenesis, we examined the expression of Nav1.6 at protein
            and mRNA levels in two distinct models of temporal lobe epilepsy
            including a post-status epilepticus model induced by kainic acid
            intrahippocampal injection and a kindling model evoked by
            pentylenetetrazole.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6548)
  AUTHORS   Dietrich,P.S., McGivern,J.G., Delgado,S.G., Koch,B.D., Eglen,R.M.,
            Hunter,J.C. and Sangameswaran,L.
  TITLE     Functional analysis of a voltage-gated sodium channel and its
            splice variant from rat dorsal root ganglia
  JOURNAL   J Neurochem 70 (6), 2262-2272 (1998)
   PUBMED   9603190
REFERENCE   7  (bases 1 to 6548)
  AUTHORS   Burgess,D.L., Kohrman,D.C., Galt,J., Plummer,N.W., Jones,J.M.,
            Spear,B. and Meisler,M.H.
  TITLE     Mutation of a new sodium channel gene, Scn8a, in the mouse mutant
            'motor endplate disease'
  JOURNAL   Nat Genet 10 (4), 461-465 (1995)
   PUBMED   7670495
REFERENCE   8  (bases 1 to 6548)
  AUTHORS   Schaller,K.L., Krzemien,D.M., Yarowsky,P.J., Krueger,B.K. and
            Caldwell,J.H.
  TITLE     A novel, abundant sodium channel expressed in neurons and glia
  JOURNAL   J Neurosci 15 (5 Pt 1), 3231-3242 (1995)
   PUBMED   7751906
REFERENCE   9  (bases 1 to 6548)
  AUTHORS   Sills,M.N., Xu,Y.C., Baracchini,E., Goodman,R.H., Cooperman,S.S.,
            Mandel,G. and Chien,K.R.
  TITLE     Expression of diverse Na+ channel messenger RNAs in rat myocardium.
            Evidence for a cardiac-specific Na+ channel
  JOURNAL   J Clin Invest 84 (1), 331-336 (1989)
   PUBMED   2544627
REFERENCE   10 (bases 1 to 6548)
  AUTHORS   Tolan,D.R., Niclas,J., Bruce,B.D. and Lebo,R.V.
  TITLE     Evolutionary implications of the human aldolase-A, -B, -C, and
            -pseudogene chromosome locations
  JOURNAL   Am J Hum Genet 41 (5), 907-924 (1987)
   PUBMED   3674018
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 27, 2020 this sequence version replaced NM_019266.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF049239.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760393,
                                           SAMEA5760433 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-117               JAXUCZ010000007.1  133861227-133861343
            118-444             JAXUCZ010000007.1  133921968-133922294
            445-563             JAXUCZ010000007.1  133940769-133940887
            564-653             JAXUCZ010000007.1  133941943-133942032
            654-782             JAXUCZ010000007.1  133942491-133942619
            783-874             JAXUCZ010000007.1  133944654-133944745
            875-1096            JAXUCZ010000007.1  133954556-133954777
            1097-1160           JAXUCZ010000007.1  133955678-133955741
            1161-1302           JAXUCZ010000007.1  133956709-133956850
            1303-1509           JAXUCZ010000007.1  133957877-133958083
            1510-1803           JAXUCZ010000007.1  133959661-133959954
            1804-2160           JAXUCZ010000007.1  133970533-133970889
            2161-2293           JAXUCZ010000007.1  133991427-133991559
            2294-2532           JAXUCZ010000007.1  133996524-133996762
            2533-2706           JAXUCZ010000007.1  133999561-133999734
            2707-3063           JAXUCZ010000007.1  134001563-134001919
            3064-3534           JAXUCZ010000007.1  134004079-134004549
            3535-3652           JAXUCZ010000007.1  134004813-134004930
            3653-3807           JAXUCZ010000007.1  134005510-134005664
            3808-3981           JAXUCZ010000007.1  134007697-134007870
            3982-4104           JAXUCZ010000007.1  134013520-134013642
            4105-4389           JAXUCZ010000007.1  134018770-134019054
            4390-4443           JAXUCZ010000007.1  134021077-134021130
            4444-4581           JAXUCZ010000007.1  134021489-134021626
            4582-4686           JAXUCZ010000007.1  134022765-134022869
            4687-4957           JAXUCZ010000007.1  134024645-134024915
            4958-6548           JAXUCZ010000007.1  134028434-134030024
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..6548
                     /gene="Scn8a"
                     /note="sodium voltage-gated channel alpha subunit 8"
                     /db_xref="GeneID:29710"
                     /db_xref="RGD:3638"
     exon            1..117
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            118..444
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..6105
                     /gene="Scn8a"
                     /note="Na+ channel; PN4; naCh6; sodium channel 6;
                     peripheral nerve protein type 4; sodium channel protein
                     type VIII subunit alpha; voltage-gated sodium channel
                     subunit alpha Nav1.6; sodium channel, voltage gated, type
                     VIII, alpha subunit; sodium channel, voltage-gated, type
                     VIII, alpha polypeptide; sodium channel, voltage-gated,
                     type 8, alpha polypeptide; sodium channel, voltage-gated,
                     type 8, alpha subunit"
                     /codon_start=1
                     /product="sodium channel protein type 8 subunit alpha"
                     /protein_id="NP_062139.2"
                     /db_xref="GeneID:29710"
                     /db_xref="RGD:3638"
                     /translation="MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSH
                     REDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKT
                     LFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKN
                     VEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSA
                     LRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGN
                     LRNKCVVWPINFNESYLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAGQCP
                     EGFQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFV
                     LVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAA
                     MATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEGEE
                     KGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIF
                     SFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQCS
                     RSSRIFPSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEATTEVEIKKKGPGSLLVS
                     MDQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKL
                     KEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEM
                     FLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELSLADVEGLSVLRSFRLLRVFKLAKSW
                     PTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINQECKLPR
                     WHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLA
                     LLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWTKVKVHAFMQAHFKQREADEVK
                     PLDELYEKKANCIANHTGVDIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNP
                     NLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEVPV
                     EQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIF
                     MILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAW
                     CWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVG
                     AIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIDIVNNKTDCEKLM
                     EGNSTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNI
                     YMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLG
                     SKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENIL
                     YWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVS
                     PTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGM
                     SNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHP
                     GSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFE
                     TFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHC
                     LDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVL
                     QRAYRGHLARRGFICRKMASNKLENGGTHRDKKESTPSTASLPSYDSVTKPDKEKQQR
                     AEEGRRERAKRQKEVRESKC"
     exon            445..563
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            564..653
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            654..782
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            783..874
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1096
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1160
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1302
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1509
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1803
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..2160
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2293
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2532
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2706
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..3063
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3534
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            3535..3652
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            3653..3807
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            3808..3981
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            3982..4104
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4389
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4390..4443
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4444..4581
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4582..4686
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4687..4957
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
     exon            4958..6548
                     /gene="Scn8a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccaagatggc gcccaccgca gtcccgcccg ccgcagcctc ggcgcctctg cagtccggcc
       61 gcgcctcccg ggccccgcgc tagggccgct gccgcctcgc ccgccgccgc cgccgccagc
      121 tgacctgtcc cggacacata actaacgaag ctgctgcagg atgagaagat ggcagcgcgg
      181 ctgctcgcac caccaggccc tgatagtttc aagcctttca cccctgagtc gctggcaaac
      241 atcgagaggc gtattgccga gagcaagctc aagaaaccac caaaggcgga tggcagccac
      301 cgggaagacg atgaagacag caagcccaag ccaaacagtg acctggaggc tgggaagagt
      361 ttgcctttca tctacgggga catcccgcaa ggcctggttg cggttcccct ggaggacttt
      421 gacccttact atttgacgca gaaaaccttt gtagtattaa acagagggaa aactctcttc
      481 agatttagtg ccacacctgc cttgtacatt ttaagccctt ttaacctgat aagaagaata
      541 gctattaaaa ttttgataca ctcagttttc agcatgatca tcatgtgcac catcctgacc
      601 aactgtgtgt tcatgacctt tagtaaccct ccagaatggt ccaagaatgt ggagtacaca
      661 ttcacaggga tttacacatt tgaatcacta gtgaaaatca tcgcaagagg tttctgcata
      721 gacggcttca ccttcttacg agacccgtgg aactggttag acttcagtgt catcatgatg
      781 gcatatgtga cagagtttgt ggacctgggc aatgtctcag cgctgagaac attcagggtt
      841 ctccgagctt tgaaaactat ctctgtaatt ccaggcctga agacaatcgt gggcgcccta
      901 atccagtccg tgaagaagct gtcggacgtg atgatcctga cagtgttctg cctgagtgtt
      961 ttcgccctga ttggcctgca gctcttcatg gggaaccttc gaaacaagtg tgtcgtgtgg
     1021 cccataaact tcaacgagag ctacctggag aacggcacca gaggctttga ctgggaggag
     1081 tatatcaaca ataaaacaaa cttttacatg gttcctggca tgctagaacc cttgctctgc
     1141 gggaacagtt ctgatgctgg gcaatgccca gagggattcc agtgcatgaa agcaggaagg
     1201 aaccccaact acggttacac cagctttgac accttcagct gggccttctt ggcattattc
     1261 cgccttatga cccaggacta ttgggagaac ttataccagc tgaccttacg agccgctggg
     1321 aaaacgtaca tgatcttctt tgtcttggtc atcttcgtgg gttctttcta tctggtgaac
     1381 ttgatcttgg ctgtggtggc catggcttat gaggaacaga accaggcaac actggaggag
     1441 gcagagcaaa aagaggccga gttcaaggca atgctggagc aactcaagaa gcagcaggag
     1501 gaggcacagg ctgctgcaat ggccacctca gcgggcactg tctcggaaga cgccattgaa
     1561 gaagaagggg aagatggggt aggctctccg aggagctcat ctgaactgtc taaactcagt
     1621 tccaagagcg cgaaggagcg gcggaaccga cggaagaaga ggaagcagaa ggagctctct
     1681 gaaggcgagg agaaagggga cccagagaag gtgtttaagt cagagtcgga agacggtatg
     1741 agaaggaagg ccttccggct gccagacaac aggataggga ggaagttttc catcatgaat
     1801 cagtcgctgc tcagcattcc aggctcgccc ttcctctccc gacataacag caaaagcagc
     1861 atcttcagct tccggggacc cggtcggttc cgggaccccg gctctgagaa tgagttcgca
     1921 gacgatgaac acagcaccgt ggaggagagc gagggccggc gtgactcgct cttcatcccg
     1981 atccgcgccc gcgagcgccg cagcagctac agtggctaca gcggctacag ccagtgcagc
     2041 cgctcgtcgc gcatcttccc cagcctgcgg cgcagcgtga agcgcaacag cacggtggac
     2101 tgcaacggcg tggtgtcact catcgggccc ggctcacaca tcgggcggct cctgcctgag
     2161 gcaacgactg aggtggaaat taagaagaaa ggccctggat ctcttttagt ttctatggac
     2221 caactcgcct cctacggacg gaaggacaga atcaacagca taatgagcgt ggtcacaaac
     2281 acgctagtgg aagagctgga agagtctcag agaaagtgcc caccgtgctg gtataagttt
     2341 gccaacactt tcctcatctg ggagtgtcac ccctactgga taaaactgaa ggagatcgtg
     2401 aacttaatcg tcatggaccc ttttgtagac ttagccatca ccatctgcat cgttctgaat
     2461 acgctattta tggcaatgga gcaccatccc atgacaccac agttcgaaca cgtcttggcc
     2521 gtaggaaatc tggtgttcac cgggatcttc acggcggaaa tgtttctgaa gctcatagcc
     2581 atggacccct actattattt ccaagaaggc tggaacattt ttgacggatt tattgtctcc
     2641 ctcagtttaa tggagctgag tctcgcagat gtggaggggc tctcagtgct gcggtctttc
     2701 cgactgctcc gagtcttcaa gctggccaag tcctggccca ccctgaacat gctgatcaag
     2761 atcatcggga actccgtggg tgccctgggc aacctgaccc tggtgctggc catcatcgtc
     2821 ttcatcttcg ccgtggtggg gatgcagctg tttggaaaga gttacaagga gtgcgtctgt
     2881 aagatcaacc aggagtgcaa gctcccgcgc tggcacatga acgacttctt ccactccttc
     2941 ctcatcgtct tccgagtgct gtgtggggag tggatcgaga ccatgtggga ctgcatggag
     3001 gtggccggcc aggccatgtg cctcattgtc ttcatgatgg ttatggtcat tggcaacctg
     3061 gtggtgctga acctattcct ggccttgctt ctgagctcct tcagcgcaga caacctggcg
     3121 gccacagacg acgacgggga aatgaacaac ctgcagatct cagtgatccg gatcaagaag
     3181 ggcgtggcct ggaccaaagt gaaggtgcac gccttcatgc aggctcactt caagcagcgg
     3241 gaggcggatg aagtgaaacc cctcgacgag ctgtatgaga agaaggccaa ctgcatcgcc
     3301 aaccacacgg gcgtggatat ccaccggaac ggcgacttcc agaagaacgg gaacggaacc
     3361 accagcggca tcggcagcag cgtggagaag tacatcatcg acgaggacca catgtccttc
     3421 attaacaacc caaacctgac cgtccgggtg cccattgctg tgggcgagtc tgacttcgag
     3481 aacctcaaca cagaggatgt tagcagcgaa tcagaccctg aaggcagcaa agataaactg
     3541 gacgatacca gctcctcaga aggaagtacc atcgacatca agcctgaggt ggaagaagtt
     3601 cccgtggagc aacctgagga atacttggat ccggacgcct gctttacaga gggttgcgtc
     3661 cagcggttca agtgctgcca ggtcaacatc gaggaaggac taggcaagtc gtggtggatc
     3721 ttgcggaaaa cctgcttcct cattgtggag cacaattggt ttgagacctt catcatcttc
     3781 atgattctgc tcagcagtgg cgccctggcc tttgaggaca tctacattga gcagaggaag
     3841 accatccgca ccatcctgga gtatgcggac aaggtcttca cctacatctt catcctggag
     3901 atgttgctca agtggacagc ctacggcttc gtcaagttct tcaccaatgc ctggtgctgg
     3961 ttggacttcc tcattgtggc tgtctcttta gtcagcctta tagctaatgc cctgggctac
     4021 tcggaactag gtgccataaa gtcccttagg accctaagag ctttgagacc cttaagagcc
     4081 ttatcacgat ttgaagggat gagggtggtg gtgaatgcct tggtgggcgc catcccctcc
     4141 atcatgaatg tgctgctggt gtgtctcatc ttctggctga ttttcagcat catgggagtt
     4201 aacctgtttg cggggaaata ccactactgc tttaacgaga cttctgaaat ccggttcgaa
     4261 atcgatattg tcaacaataa aacggactgt gagaagctca tggagggcaa cagcacggag
     4321 atccgatgga agaatgtcaa gatcaacttt gacaatgtcg gagcagggta cctggccctt
     4381 cttcaagtgg caaccttcaa aggctggatg gacatcatgt atgcggctgt agattcccga
     4441 aagccagacg agcagcctga ctacgagggc aacatctaca tgtacatcta cttcgtcatc
     4501 ttcatcatct tcggctcctt cttcaccctc aacctgttca tcggtgtcat catcgacaac
     4561 ttcaaccagc agaagaaaaa gtttggaggt caggacatct tcatgacaga ggaacagaag
     4621 aagtactaca atgccatgaa aaagctgggc tccaagaagc cacagaagcc catcccccga
     4681 cccttgaaca aaatccaagg gattgtcttt gatttcgtca ctcaacaagc ctttgacatt
     4741 gtgatcatga tgctcatctg ccttaacatg gtgacaatga tggtggagac agacactcag
     4801 agcaagcaga tggagaacat tctttactgg attaatctgg tctttgtcat cttcttcacc
     4861 tgcgagtgtg tgctcaaaat gtttgccttg agacactact atttcaccat tggctggaac
     4921 atctttgact ttgtggtggt catcctctcc attgtgggaa tgttcctggc tgatatcatt
     4981 gagaagtact tcgtctcccc aaccctattc cgagttatcc gattggcccg tattgggcgc
     5041 atcttgcgtc tgatcaaggg cgccaaaggg atccgcaccc tgctctttgc cttaatgatg
     5101 tcgctgcccg ccctgttcaa catcggcctc ctgctcttcc tcgtcatgtt catcttctcc
     5161 atttttggca tgtccaactt cgcatacgtg aagcacgagg ccggcattga cgacatgttc
     5221 aacttcgaga catttggcaa cagcatgatc tgtttgttcc agatcacaac gtctgctggc
     5281 tgggatggcc tgctgctgcc aatcctgaac cgcccccctg actgcagctt ggacaaagag
     5341 cacccaggga gtggcttcaa aggggactgt gggaacccct cggtgggcat cttcttcttt
     5401 gtgagctaca tcatcatctc cttcctgatt gtggtgaaca tgtacatcgc catcatcctg
     5461 gagaacttca gcgtggccac cgaggagagc gccgaccctc tgagtgagga tgacttcgag
     5521 actttctatg agatctggga gaagtttgac ccagacgcca cccagttcat cgagtactgt
     5581 aagctggcag actttgccga cgccctggag cacccgctcc gagtacccaa gcccaacacc
     5641 atcgagctca tcgccatgga cctgcccatg gtgagcggag atcgcatcca ctgcttggac
     5701 atccttttcg ccttcaccaa gcgagtcctg ggagacagtg gggagttgga catcctgcgg
     5761 cagcagatgg aggagcggtt cgtggcatcc aatccttcca aagtgtctta cgagcctatc
     5821 acaaccactc tgcggcgcaa gcaggaggag gtgtctgcag tggtcctgca gcgtgcctac
     5881 aggggacact tggctaggcg gggcttcatc tgcagaaaga tggcctccaa caagctggag
     5941 aatggaggca cacacagaga caagaaggag agcaccccgt ccacagcctc cctcccctct
     6001 tacgacagcg tcacaaagcc agacaaggag aagcagcagc gtgcggagga gggcagaagg
     6061 gaaagagcca agaggcaaaa agaggtcagg gagtccaagt gctagaggag gggaaaggaa
     6121 gcttaccccg gctgaacact ggcaagtgaa agcttgttta caaacttccg aatctcacgg
     6181 atgcagagca gctgtgcaga cgctcgctgt actggaagac ctataccaaa catagtctgc
     6241 ttacatgtga catggtggca tcctgagcgg tgactgctgg ggacaaagga ccctgctccc
     6301 tggactcaca gatctcctat cgcttgggca gacggttact gcatgttcca cacttagtca
     6361 atgcaactta ggactaaact aaccaggata caaaaccgag gcggctgccg ggaccagcag
     6421 atcaccgctg cagccaaatg gattttattt tttcattttg ttgattctca gaagcagaaa
     6481 gcatcacttt aaaagtttgt ttgttcatgc aaacaatatt tgaattctta cattagttaa
     6541 gctaagca
//
