LOCUS       NM_013178               6959 bp    mRNA    linear   ROD 13-OCT-2024
DEFINITION  Rattus norvegicus sodium voltage-gated channel alpha subunit 4
            (Scn4a), mRNA.
ACCESSION   NM_013178
VERSION     NM_013178.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Pan,X., Li,Z., Zhou,Q., Shen,H., Wu,K., Huang,X., Chen,J.,
            Zhang,J., Zhu,X., Lei,J., Xiong,W., Gong,H., Xiao,B. and Yan,N.
  TITLE     Structure of the human voltage-gated sodium channel Nav1.4 in
            complex with beta1
  JOURNAL   Science 362 (6412) (2018)
   PUBMED   30190309
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Sanchez-Solano,A., Islas,A.A., Scior,T., Paiz-Candia,B.,
            Millan-PerezPena,L. and Salinas-Stefanon,E.M.
  TITLE     Characterization of specific allosteric effects of the Na+ channel
            beta1 subunit on the Nav1.4 isoform
  JOURNAL   Eur Biophys J 46 (5), 485-494 (2017)
   PUBMED   28012039
  REMARK    GeneRIF: the role of the intracellular IFM motif of rNav1.4
            (skeletal muscle isoform of the rat Na(+) channel) on the
            alpha-beta1 functional interaction
REFERENCE   3  (bases 1 to 6959)
  AUTHORS   Kubota,T., Durek,T., Dang,B., Finol-Urdaneta,R.K., Craik,D.J.,
            Kent,S.B., French,R.J., Bezanilla,F. and Correa,A.M.
  TITLE     Mapping of voltage sensor positions in resting and inactivated
            mammalian sodium channels by LRET
  JOURNAL   Proc Natl Acad Sci U S A 114 (10), E1857-E1865 (2017)
   PUBMED   28202723
  REMARK    GeneRIF: Intramolecular distances computed from the LRET signals
            define a geometrical map of Nav1.4 with the bound toxins, and
            reveal voltage-dependent structural changes related to channel
            gating
REFERENCE   4  (bases 1 to 6959)
  AUTHORS   Sheets,M.F., Fozzard,H.A. and Hanck,D.A.
  TITLE     Important Role of Asparagines in Coupling the Pore and
            Votage-Sensor Domain in Voltage-Gated Sodium Channels
  JOURNAL   Biophys J 109 (11), 2277-2286 (2015)
   PUBMED   26636939
  REMARK    GeneRIF: Role of Asparagines in Coupling the Pore and Votage-Sensor
            Domain in Voltage-Gated Sodium Channels
REFERENCE   5  (bases 1 to 6959)
  AUTHORS   Mahdavi,S. and Kuyucak,S.
  TITLE     Molecular dynamics study of binding of micro-conotoxin GIIIA to the
            voltage-gated sodium channel Na(v)1.4
  JOURNAL   PLoS One 9 (8), e105300 (2014)
   PUBMED   25133704
  REMARK    GeneRIF: The study constructs a homology model for the pore domain
            of the NaV1.4 channel and uses the functional data for the binding
            of micro-conotoxin GIIIA to NaV1.4 to validate the model.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6959)
  AUTHORS   Tsujino,A., Maertens,C., Ohno,K., Shen,X.M., Fukuda,T.,
            Harper,C.M., Cannon,S.C. and Engel,A.G.
  TITLE     Myasthenic syndrome caused by mutation of the SCN4A sodium channel
  JOURNAL   Proc Natl Acad Sci U S A 100 (12), 7377-7382 (2003)
   PUBMED   12766226
REFERENCE   7  (bases 1 to 6959)
  AUTHORS   Carbonneau,E., Vijayaragavan,K. and Chahine,M.
  TITLE     A tryptophan residue (W736) in the amino-terminus of the P-segment
            of domain II is involved in pore formation in Na(v)1.4
            voltage-gated sodium channels
  JOURNAL   Pflugers Arch 445 (1), 18-24 (2002)
   PUBMED   12397382
  REMARK    GeneRIF: Role of a conserved neighbouring tryptophan residue at
            position 736 (W736) of Na(V)1.4 in the pore formation was examined.
            W736 contributes to the formation of the pore, close to the mouth
            of the channel, but is not part of the selectivity filter.
REFERENCE   8  (bases 1 to 6959)
  AUTHORS   Zimmer,T., Bollensdorff,C., Haufe,V., Birch-Hirschfeld,E. and
            Benndorf,K.
  TITLE     Mouse heart Na+ channels: primary structure and function of two
            isoforms and alternatively spliced variants
  JOURNAL   Am J Physiol Heart Circ Physiol 282 (3), H1007-H1017 (2002)
   PUBMED   11834499
REFERENCE   9  (bases 1 to 6959)
  AUTHORS   Diss,J.K., Stewart,D., Fraser,S.P., Black,J.A., Dib-Hajj,S.,
            Waxman,S.G., Archer,S.N. and Djamgoz,M.B.
  TITLE     Expression of skeletal muscle-type voltage-gated Na+ channel in rat
            and human prostate cancer cell lines
  JOURNAL   FEBS Lett 427 (1), 5-10 (1998)
   PUBMED   9613589
REFERENCE   10 (bases 1 to 6959)
  AUTHORS   Trimmer,J.S., Cooperman,S.S., Tomiko,S.A., Zhou,J.Y., Crean,S.M.,
            Boyle,M.B., Kallen,R.G., Sheng,Z.H., Barchi,R.L., Sigworth,F.J. et
            al.
  TITLE     Primary structure and functional expression of a mammalian skeletal
            muscle sodium channel
  JOURNAL   Neuron 3 (1), 33-49 (1989)
   PUBMED   2559760
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Nov 26, 2020 this sequence version replaced NM_013178.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M26643.1, SRR26643296.28869.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                JAXUCZ010000010.1  91796275-91796324   c
            51-722              JAXUCZ010000010.1  91779871-91780542   c
            723-841             JAXUCZ010000010.1  91779278-91779396   c
            842-931             JAXUCZ010000010.1  91779081-91779170   c
            932-1060            JAXUCZ010000010.1  91778656-91778784   c
            1061-1152           JAXUCZ010000010.1  91778144-91778235   c
            1153-1467           JAXUCZ010000010.1  91775897-91776211   c
            1468-1531           JAXUCZ010000010.1  91774692-91774755   c
            1532-1673           JAXUCZ010000010.1  91774340-91774481   c
            1674-1883           JAXUCZ010000010.1  91772599-91772808   c
            1884-2037           JAXUCZ010000010.1  91771707-91771860   c
            2038-2276           JAXUCZ010000010.1  91769063-91769301   c
            2277-2450           JAXUCZ010000010.1  91767073-91767246   c
            2451-2807           JAXUCZ010000010.1  91765255-91765611   c
            2808-3281           JAXUCZ010000010.1  91759954-91760427   c
            3282-3417           JAXUCZ010000010.1  91757951-91758086   c
            3418-3572           JAXUCZ010000010.1  91757432-91757586   c
            3573-3746           JAXUCZ010000010.1  91755746-91755919   c
            3747-3869           JAXUCZ010000010.1  91755183-91755305   c
            3870-4148           JAXUCZ010000010.1  91753414-91753692   c
            4149-4202           JAXUCZ010000010.1  91753121-91753174   c
            4203-4340           JAXUCZ010000010.1  91752781-91752918   c
            4341-4445           JAXUCZ010000010.1  91751835-91751939   c
            4446-4716           JAXUCZ010000010.1  91750202-91750472   c
            4717-6959           JAXUCZ010000010.1  91746715-91748957   c
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.1"
     gene            1..6959
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /note="sodium voltage-gated channel alpha subunit 4"
                     /db_xref="GeneID:25722"
                     /db_xref="RGD:3636"
     exon            1..50
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            51..722
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..5972
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /note="sodium channel, voltage-gated, type 4, alpha
                     polypeptide; mu-1; skM1; sodium channel, voltage-gated,
                     type IV, alpha subunit; sodium channel protein type IV
                     subunit alpha; voltage-gated sodium channel subunit alpha
                     Nav1.4; sodium channel protein skeletal muscle subunit
                     alpha; sodium channel, voltage-gated, type IV, alpha
                     polypeptide; sodium channel, voltage-gated, type 4, alpha
                     subunit; Sodium channel voltage-gated type IV alpha
                     polypeptide"
                     /codon_start=1
                     /product="sodium channel protein type 4 subunit alpha"
                     /protein_id="NP_037310.2"
                     /db_xref="GeneID:25722"
                     /db_xref="RGD:3636"
                     /translation="MASSSLPNLVPPGPHCLRPFTPESLAAIEQRAVEEEARLQRNKQ
                     MEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGIPLEDLDPYYSDKKTFIVLNKGKAI
                     FRFSATPALYLLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKHV
                     EYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISAL
                     RTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNL
                     RQKCVRWPPPMNDTNTTWYGNDTWYSNDTWYGNDTWYINDTWNSQESWAGNSTFDWEA
                     YINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLA
                     LFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEA
                     TLAEDQEKEEEFQQMLEKYKKHQEEVEKAKAAQALESGEEADGDPTHNKDCNGSLDAS
                     GEKGPPRPSCSADSAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIIY
                     LIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLI
                     AMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNM
                     LIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCNLPRWHMND
                     FFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSS
                     FSADSLAASDEDGEMNNLQIAIGRIKWGIGFAKTFLLGLLRGKILSPKEIILSLGEPG
                     GAGENAEESTPEDEKKEPPPEDKELKDNHILNHVGLTDGPRSSIELDHLNFINNPYLT
                     IHVPIASEESDLEMPTEEETDAFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEE
                     QAEENPEGEQPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFI
                     VFMILLSSGALAFEDIYIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTN
                     AWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNAL
                     LGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTSERFDISVVNNKSECES
                     LMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNL
                     YMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLG
                     SKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDIL
                     YNINMVFIIIFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVS
                     PTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGM
                     SNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLEN
                     PGTNVRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLSEDDF
                     EMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIH
                     CLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIK
                     IQRAYRRHLLQRSVKQASYMYRHSQDGNGDGAPEKEGLLANTMNKMYGHEKEGDGVQS
                     QGEEEKASTEDAGPTVEPKPTSSSDTALTPSPPPLPPSSSPPQGQTVRPGVKESLV"
     exon            723..841
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            842..931
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1060
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1152
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1467
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1531
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1673
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1883
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2037
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2276
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2450
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2807
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..3281
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3417
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3572
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            3573..3746
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            3747..3869
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            3870..4148
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            4149..4202
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4340
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            4341..4445
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            4446..4716
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
     exon            4717..6959
                     /gene="Scn4a"
                     /gene_synonym="microI; Nav1.4; NCHVS"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acggcacccg gggctcgtcc gccaccgctc ttctctgctt ctggccaaca gctgaagtca
       61 cgcagttcct ggagtctgaa cccaggagac agttcgggac actggggcgt tggccccaag
      121 ctcctcaggc tggagaagat ctggttcact ttctgttgat cccgaaggtg gcacaatcta
      181 ttgctgagaa gagaacactg cggggcctgg agtgggggaa tgcaccgtgc ccagtgtcct
      241 gggcatcagc acgcccaggt cttgcaggca catctcccag ccccaacaag accaggacac
      301 ccacctccct gacccaactc ctgggcaggc agagagtgag gagtacacag tagaccccgg
      361 gcattggtga ccacccaaaa cccaagggct gcgggctgta gcggctctgt tacctaccac
      421 cctctggccc ctgagcccag aatgcaagga tggccagctc atctctgccc aacctggtcc
      481 ccccgggtcc ccactgcctg cgccccttca ccccagagtc cctggcagcc atagagcagc
      541 gggcggtgga ggaggaggcc cggctgcagc ggaacaagca gatggagatt gaggagcctg
      601 agcggaagcc acgcagtgac ctggaagctg gcaagaacct cccactcatc tatggggacc
      661 ccccacccga agtcattggc atccccctgg aggacctgga tccttactac agtgacaaga
      721 agaccttcat tgtgctcaac aaaggaaagg ccatcttccg attctctgcc acgcctgccc
      781 tctacctgct gagccccttc agcatcgtca ggagggtggc tatcaaggtg ctcattcacg
      841 cgctgttcag catgtttatc atgatcacca tcctgaccaa ctgtgtgttc atgaccatga
      901 gcaatccgcc ttcttggtcc aaacacgtgg agtacacctt cacggggatc tatacctttg
      961 agtccctcat taagatgctg gcccgaggct tttgcattga tgacttcaca ttcctccgag
     1021 acccctggaa ctggctggac ttcagtgtca tcacaatggc gtatgtgaca gagtttgtgg
     1081 acttgggcaa catctcagcc ctgaggacct tccgtgtgct gcgggccctg aagaccatca
     1141 cggttatccc agggctgaag acaattgtgg gagccctgat ccagtctgtg aaaaagctgt
     1201 cggatgtgat gatcctcact gtcttctgcc tgagtgtctt tgccctggtg gggctgcagc
     1261 ttttcatggg aaacctgcgt cagaagtgcg tgcgttggcc cccgcccatg aatgacacca
     1321 acaccacgtg gtatggcaat gacacttggt acagcaatga cacttggtac ggcaatgaca
     1381 cttggtacat caatgacact tggaacagcc aggagagctg ggccggcaac tctacctttg
     1441 actgggaggc ctacatcaat gacgaaggga acttctattt cttggagggc tccaatgatg
     1501 ctctgctctg tgggaatagc agtgatgctg ggcactgccc tgagggctac gaatgcataa
     1561 aggctgggcg gaaccccaac tatggctaca ccagctatga caccttcagc tgggctttcc
     1621 tggctctctt ccggctcatg acgcaggact actgggagaa ccttttccag ctgaccctac
     1681 gagctgctgg caagacctac atgatcttct tcgtggtcat catcttcctg ggctccttct
     1741 acctcatcaa tctgatcctg gccgtggtgg ccatggcgta cgctgagcag aatgaggcta
     1801 ccctggccga agaccaggag aaagaggagg agttccaaca gatgcttgag aaatacaaaa
     1861 aacatcagga ggaagtggaa aaggctaagg ctgcccaggc tctggaaagt ggagaggagg
     1921 cagatgggga cccaacccac aacaaagact gcaatgggag cctggatgca tccggggaga
     1981 aggggccccc aaggccaagc tgcagcgcag acagtgccat ctcagatgct atggaggagc
     2041 tggaagaggc ccatcagaag tgcccaccgt ggtggtacaa gtgtgcacac aaagtcctca
     2101 tctggaactg ctgtgccccg tgggtgaagt tcaaacatat aatctacctg atcgtcatgg
     2161 acccctttgt ggacctgggt atcaccatct gcattgtgct caacaccctc ttcatggcca
     2221 tggagcacta ccccatgacc gagcactttg acaacgtgct ctccgtgggc aacttggtct
     2281 tcacaggcat cttcactgcg gagatggtgt tgaagctgat tgccatggac ccctacgagt
     2341 atttccaaca gggctggaac atctttgaca gtttcatcgt caccctcagc ctggtggagc
     2401 tgggcctggc caacgtacag gggctgtcag tgctccgttc cttccgcctg ctgcgtgtct
     2461 tcaagctggc caagtcatgg ccaacactca acatgctcat caaaatcatt ggcaactcag
     2521 tgggcgcgct gggcaacctg accctggtgc tggccatcat cgtcttcatc ttcgccgtgg
     2581 tgggcatgca gctgttcggc aagagctata aggagtgtgt gtgcaagatc gcctcagact
     2641 gcaacctgcc tcgctggcac atgaacgact tcttccactc cttcctcatc gtcttccgca
     2701 tcctctgcgg ggaatggatc gagaccatgt gggactgcat ggaggtggcc ggccaggcca
     2761 tgtgcctcac cgtcttcctc atggtcatgg tcattggcaa cctggtggtc ctgaatctgt
     2821 tcctggctct cctgctgagt tccttcagtg ctgacagcct ggcggcctcg gatgaggatg
     2881 gcgagatgaa caacctacag attgccatcg ggcgtatcaa gtggggcatc ggttttgcca
     2941 aaaccttcct cctggggctg ttgcgtggca agatcctgag ccccaaggaa ataatactca
     3001 gcctcggtga gcccgggggt gccggggaaa atgccgagga gagtactccc gaggatgaga
     3061 agaaggagcc gccaccggaa gataaggagc tgaaagacaa tcatatcctg aaccacgtgg
     3121 gcctgaccga tggcccccgc tccagcatcg agctggacca ccttaacttc atcaacaacc
     3181 cctacctcac catccacgtg cccattgcct ccgaggagtc tgacctggag atgcccacag
     3241 aggaggagac agacgccttc tcggagcctg aggatatcaa gaagccccta cagcccctct
     3301 acgacgggaa ctcctccgtc tgcagcacag ctgactacaa gccccctgaa gaggaccccg
     3361 aggagcaggc tgaggagaac cccgaggggg agcagcctga ggaatgcttc acggaagcct
     3421 gtgtgaagcg ctgcccctgc ctctatgtgg acatctccca gggccgtggg aaaatgtggt
     3481 ggacactgcg cagggcctgt ttcaagattg ttgagcacaa ctggtttgag accttcattg
     3541 tcttcatgat cttgctcagc agtggagccc tggccttcga agacatctac attgaacagc
     3601 gacgagtcat ccgcaccatc ctggaatacg ccgacaaggt cttcacctac atcttcatcc
     3661 tggagatgtt gctaaagtgg gtcgcctacg gcttcaaggt gtatttcacc aatgcctggt
     3721 gctggctcga cttcctcatc gtggacgtct ccatcatcag cctggtggca aactggctgg
     3781 gctactctga gctggggccc atcaaatccc tgcgcacact ccgggccctg cgacctctga
     3841 gggcactgtc tcgatttgag ggtatgaggg tggtggtgaa cgccctcctg ggagctatcc
     3901 cctccatcat gaacgtcctc ctcgtctgcc tcatcttctg gctcatcttc agcatcatgg
     3961 gggtcaactt gtttgctgga aagttctact actgcgtcaa caccaccacc tctgagagat
     4021 tcgacatctc ggtggtcaac aacaagtccg agtgtgagag cctcatgtat acaggccagg
     4081 tccgctggat gaatgtcaag gtcaactacg acaacgtggg tctgggctat ctctccctcc
     4141 ttcaggtggc cacattcaag ggttggatgg atatcatgta tgcagctgtg gactcccggg
     4201 agaaagagga gcagccagac tatgaggtga acctctacat gtacctctac ttcgtcatct
     4261 tcatcatctt cggctccttc ttcaccctca acctcttcat cggtgtcatc atcgacaact
     4321 tcaaccaaca gaagaagaag tttggaggga aagacatctt catgacagag gaacagaaga
     4381 aatactacaa cgcgatgaag aagcttggct ccaagaagcc ccagaagcca attccccggc
     4441 ctcagaacaa gatccagggc atggtgtacg acttcgtgac gaagcaggtg tttgacatct
     4501 ccatcatgat cctcatctgc ctcaacatgg ttactatgat ggtggagaca gatgaccaga
     4561 gccagctcaa ggtggacatc ctgtacaaca tcaacatggt cttcattatc atcttcacag
     4621 gggagtgtgt gctgaagatg tttgccctac gccattacta cttcaccatt ggctggaaca
     4681 tctttgactt tgtggttgtc atcttgtcca tcgtgggcct tgcgctctct gacttgatac
     4741 agaaatactt tgtgtcaccc acgctgttcc gtgtgatccg cttggctcgg atcgggcgtg
     4801 tcctgcgtct gatccgcggg gccaagggca tccggacgct gctgttcgcc ctgatgatgt
     4861 ccctgcccgc cctcttcaac atcggcctcc ttctctttct ggtcatgttc atctactcga
     4921 tcttcggcat gtctaacttc gcctacgtca agaaagagtc aggcatcgac gacatgttta
     4981 actttgagac ctttggcaac agtatcatct gcctcttcga gatcaccacg tcagccggct
     5041 gggacgggct tctgaacccc atcctcaaca gtgggccccc agactgtgac ccgacgttgg
     5101 agaacccggg taccaatgtc aggggggact gcggcaaccc ttccatcggc atctgtttct
     5161 tctgcagcta catcatcatc tccttcctca tcgtggtcaa catgtacatt gctatcatcc
     5221 tggagaattt caacgtggcc accgaagaga gcagcgagcc cctcagtgag gatgacttcg
     5281 agatgttcta tgagacctgg gagaagttcg acccggacgc cactcagttc atcgactaca
     5341 gccgcctctc ggactttgtg gacaccctgc aggagccgct gaaaatcgcc aaacccaata
     5401 agatcaagct tatcacgtta gacctgccca tggtgcccgg cgacaagatc cactgcttgg
     5461 acatcctctt tgccctgacc aaagaggtac tgggtgactc tggggagatg gacgccctca
     5521 agcagaccat ggaggagaag tttatggcag ctaacccttc caaggtctcc tatgagccca
     5581 tcaccaccac cctcaagagg aagcaggagg aggtgtgtgc tatcaaaatc cagagggcct
     5641 accgccgcca cctgctgcag cgctccgtga agcaggcctc ttacatgtac cgtcacagcc
     5701 aggacggcaa tggcgacggg gcccccgaga aggagggatt gcttgccaac accatgaaca
     5761 agatgtatgg ccatgagaaa gagggtgatg gtgtgcagag ccagggggag gaagagaagg
     5821 cctctacaga ggatgctgga cccaccgtgg agcccaaacc caccagcagc tcagatactg
     5881 ccctgactcc ttctcctcca ccactacccc cctcatcatc accgccacaa gggcagacag
     5941 ttcgcccagg ggtcaaagag tctcttgtct agatggcagc gtcaaggctg cacgcagagt
     6001 tggaggggaa gggctttgag atgggggcta tacaggactt gacacctggg ctgaggggta
     6061 tggaaattgt gctcagggtt caggtgggtc ggaagcccct gctgcctgat ttcatcttta
     6121 cgatgctctg acattctgtt ggccagggga ggccaggaca tctgaatgct gtttccttct
     6181 tgggggagag ccagcatacc ttggaggggt tggccacctt cctgtcacta ctttaagggc
     6241 ccaggacccc agctctagcc aagacaaaga cgaatcttaa cccccaaacc tacacctgac
     6301 ccccagagct tagctatccc tctcgccccc ataattctag ggtgcagctt ccccctgaca
     6361 gctcaggaat gccctgtaaa aggggaaatg gttctggtac agaaccaatt ggccgctgga
     6421 tccggatgta tcaggctgga aacatgactt ctgaaacctt tcacccagtc agatccaggc
     6481 cggtgggcag ctcccctggc tggcccctgg gggaagaact aaggaaggat tgccctgaga
     6541 aattaccttt ctctagattg cgtggatggg tcaaacaggc tgggtcctgc cagctgcatt
     6601 catgcccttg agcctgagcg cccacatctg cctggggtca ggttggaagc tctctatcaa
     6661 gaatctgccc gttcccgcct ccctgccttc tttctttgaa taccagggct cctgggagcc
     6721 ccatcaaaca cgaaagactg ccttcctcct gtctaatctc tgcccccccc cttccctgcc
     6781 ccttccctgc ttcctcaagt gactctgaga atgctagaac tcagcacacg ggagatgagt
     6841 tgatactggg gcatgcagtg agaaggctca ccaggcttgg ctttggtctt ctcattgcct
     6901 gaaagcaagt tttcccctga gacagggttt cttttctccg tgtagccctg gctgcctgg
//
