LOCUS       NM_012647               8556 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus sodium voltage-gated channel alpha subunit 2
            (Scn2a), mRNA.
ACCESSION   NM_012647
VERSION     NM_012647.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   van Cann,M., Kuzmenkov,A., Isensee,J., Andreev-Andrievskiy,A.,
            Peigneur,S., Khusainov,G., Berkut,A., Tytgat,J., Vassilevski,A. and
            Hucho,T.
  TITLE     Scorpion toxin MeuNaTxalpha-1 sensitizes primary nociceptors by
            selective modulation of voltage-gated sodium channels
  JOURNAL   FEBS J 288 (7), 2418-2435 (2021)
   PUBMED   33051988
  REMARK    GeneRIF: Scorpion toxin MeuNaTxalpha-1 sensitizes primary
            nociceptors by selective modulation of voltage-gated sodium
            channels.
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Middleton,S.J., Kneller,E.M., Chen,S., Ogiwara,I., Montal,M.,
            Yamakawa,K. and McHugh,T.J.
  TITLE     Altered hippocampal replay is associated with memory impairment in
            mice heterozygous for the Scn2a gene
  JOURNAL   Nat Neurosci 21 (7), 996-1003 (2018)
   PUBMED   29867081
REFERENCE   3  (bases 1 to 8556)
  AUTHORS   Johnson,K.W., Herold,K.F., Milner,T.A., Hemmings,H.C. Jr. and
            Platholi,J.
  TITLE     Sodium channel subtypes are differentially localized to pre- and
            post-synaptic sites in rat hippocampus
  JOURNAL   J Comp Neurol 525 (16), 3563-3578 (2017)
   PUBMED   28758202
  REMARK    GeneRIF: The Nav1.2 are highly expressed in axon terminals in
            central hilus compared to stratum radiatum and in dendrites and
            spines in stratum radiatum compared to central hilus .
REFERENCE   4  (bases 1 to 8556)
  AUTHORS   Berret,E., Barron,T., Xu,J., Debner,E., Kim,E.J. and Kim,J.H.
  TITLE     Oligodendroglial excitability mediated by glutamatergic inputs and
            Nav1.2 activation
  JOURNAL   Nat Commun 8 (1), 557 (2017)
   PUBMED   28916793
  REMARK    GeneRIF: Knockdown of Nav1.2 channels in pre-oligodendrocyte alters
            their morphology, reduces axon-oligodendrocyte interactions and
            impairs myelination.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8556)
  AUTHORS   Hovey,L., Fowler,C.A., Mahling,R., Lin,Z., Miller,M.S., Marx,D.C.,
            Yoder,J.B., Kim,E.H., Tefft,K.M., Waite,B.C., Feldkamp,M.D., Yu,L.
            and Shea,M.A.
  TITLE     Calcium triggers reversal of calmodulin on nested anti-parallel
            sites in the IQ motif of the neuronal voltage-dependent sodium
            channel NaV1.2
  JOURNAL   Biophys Chem 224, 1-19 (2017)
   PUBMED   28343066
  REMARK    GeneRIF: The polarity of (Ca(2+))2-CaMC relative to the IQ motif of
            NaV1.2 was opposite to that seen in apo CaMC-Nav1.2IQp (2KXW),
            revealing that CaMC recognizes nested, anti-parallel sites in
            Nav1.2IQp. (CaMC is C-domain residues of CaM, calmodulin).
REFERENCE   6  (bases 1 to 8556)
  AUTHORS   Sampo,B., Tricaud,N., Leveque,C., Seagar,M., Couraud,F. and
            Dargent,B.
  TITLE     Direct interaction between synaptotagmin and the intracellular loop
            I-II of neuronal voltage-sensitive sodium channels
  JOURNAL   Proc Natl Acad Sci U S A 97 (7), 3666-3671 (2000)
   PUBMED   10737807
REFERENCE   7  (bases 1 to 8556)
  AUTHORS   Szpirer,C., Szpirer,J., Van Vooren,P., Tissir,F., Simon,J.S.,
            Koike,G., Jacob,H.J., Lander,E.S., Helou,K., Klinga-Levan,K. and
            Levan,G.
  TITLE     Gene-based anchoring of the rat genetic linkage and cytogenetic
            maps: new regional localizations, orientation of the linkage
            groups, and insights into mammalian chromosome evolution
  JOURNAL   Mamm Genome 9 (9), 721-734 (1998)
   PUBMED   9716657
REFERENCE   8  (bases 1 to 8556)
  AUTHORS   Kjaergaard,J.
  TITLE     [Hospitals--new charge by physicians on equally worthy leadership]
  JOURNAL   Sygeplejersken 92 (48), 38-39 (1992)
   PUBMED   1299025
REFERENCE   9  (bases 1 to 8556)
  AUTHORS   Noda,M. and Numa,S.
  TITLE     Structure and function of sodium channel
  JOURNAL   J Recept Res 7 (1-4), 467-497 (1987)
   PUBMED   2442385
REFERENCE   10 (bases 1 to 8556)
  AUTHORS   Noda,M., Ikeda,T., Kayano,T., Suzuki,H., Takeshima,H., Kurasaki,M.,
            Takahashi,H. and Numa,S.
  TITLE     Existence of distinct sodium channel messenger RNAs in rat brain
  JOURNAL   Nature 320 (6058), 188-192 (1986)
   PUBMED   3754035
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000115.1.
            
            [WARNING] On Mar 28, 2024 this sequence was replaced by
            NM_012647.3.
            
            On Nov 27, 2020 this sequence version replaced NM_012647.1.
            
            Summary: Voltage-gated sodium channels are transmembrane
            glycoprotein complexes composed of a large alpha subunit with four
            repeat domains, each of which is composed of six membrane-spanning
            segments, and one or more regulatory beta subunits. Voltage-gated
            sodium channels are responsible for the generation and propagation
            of action potentials in neurons and muscle. This gene encodes one
            member of the sodium channel alpha subunit gene family. In humans,
            variants of this gene are associated with seizure disorders and
            autism spectrum disorder. Mice homozygous for a knockout mutation
            die with severe hypoxia and extensive neuronal cell death, while
            gain of function mutations result in progressive seizure disorder.
            [provided by RefSeq, Nov 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X03639.1, M22254.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132291, SAMD00132300
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               JACYVU010000115.1  43759700-43759855
            157-474             JACYVU010000115.1  43809661-43809978
            475-593             JACYVU010000115.1  43810811-43810929
            594-683             JACYVU010000115.1  43820176-43820265
            684-812             JACYVU010000115.1  43821142-43821270
            813-904             JACYVU010000115.1  43821648-43821739
            905-1177            JACYVU010000115.1  43822838-43823110
            1178-1241           JACYVU010000115.1  43825967-43826030
            1242-1383           JACYVU010000115.1  43827639-43827780
            1384-1590           JACYVU010000115.1  43827923-43828129
            1591-1878           JACYVU010000115.1  43829390-43829677
            1879-2226           JACYVU010000115.1  43842970-43843317
            2227-2356           JACYVU010000115.1  43848052-43848181
            2357-2595           JACYVU010000115.1  43852547-43852785
            2596-2769           JACYVU010000115.1  43854513-43854686
            2770-3126           JACYVU010000115.1  43856502-43856858
            3127-3606           JACYVU010000115.1  43861660-43862139
            3607-3727           JACYVU010000115.1  43870657-43870777
            3728-3882           JACYVU010000115.1  43872732-43872886
            3883-4056           JACYVU010000115.1  43874453-43874626
            4057-4179           JACYVU010000115.1  43877362-43877484
            4180-4461           JACYVU010000115.1  43880630-43880911
            4462-4515           JACYVU010000115.1  43881742-43881795
            4516-4653           JACYVU010000115.1  43884716-43884853
            4654-4758           JACYVU010000115.1  43885236-43885340
            4759-5029           JACYVU010000115.1  43889352-43889622
            5030-8556           JACYVU010000115.1  43890509-43894035
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q21"
     gene            1..8556
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /note="sodium voltage-gated channel alpha subunit 2"
                     /db_xref="GeneID:24766"
                     /db_xref="RGD:3632"
     exon            1..156
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            157..474
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     CDS             208..6225
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /note="sodium channel voltage-gated type II alpha
                     polypeptide; RIIA sodium channel protein; sodium channel
                     protein type II subunit alpha; sodium channel protein,
                     brain II subunit alpha; voltage-gated sodium channel
                     subunit alpha Nav1.2; sodium channel, voltage-gated, type
                     2, alpha 1 polypeptide; sodium channel, voltage-gated,
                     type II, alpha subunit; sodium channel, voltage-gated,
                     type 2, alpha 1 subunit; sodium channel, voltage-gated,
                     type II, alpha 1"
                     /codon_start=1
                     /product="sodium channel protein type 2 subunit alpha"
                     /protein_id="NP_036779.2"
                     /db_xref="GeneID:24766"
                     /db_xref="RGD:3632"
                     /translation="MARSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDE
                     DDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISR
                     FSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEY
                     TFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVNLGNVSALRT
                     FRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRN
                     KCLQWPPDNSTFEINITSFFNNSLDWNGTAFNRTVNMFNWDEYIEDKSHFYFLEGQND
                     ALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQL
                     TLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQML
                     EQLKKQQEEAQAAAAAASAESRDFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKK
                     KQKEQAGEEEKEDAVRKSASEDSIRKKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSL
                     FSPRRNSRASLFSFKGRVKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRPSN
                     VSQASRASRGIPTLPMNGKMHSAVDCNGVVSLVGGPSALTSPVGQLLPEGTTTETEIR
                     KRRSSSYHVSMDLLEDPSRQRAMSMASILTNTMEELEESRQKCPPCWYKFANMCLIWD
                     CCKPWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVF
                     TGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGLANVEGLSVLRSFRLLR
                     VFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI
                     SNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNL
                     VVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDFVKRKIREFIQKAFV
                     RKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVD
                     ESDYMSFINNPSLTVTVPIALGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTV
                     DIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKI
                     VEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWV
                     AYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRF
                     EGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVS
                     VVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRN
                     VELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQ
                     KKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVET
                     DDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMF
                     LAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLF
                     LVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNS
                     GPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATE
                     ESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAM
                     DLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTL
                     KRKQEEVSAIVIQRAYRRYLLKQKVKKVSSIYKKDKGKEDEGTPIKEDIITDKLNENS
                     TPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK"
     exon            475..593
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            594..683
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            684..812
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            813..904
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1177
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1241
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1383
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1590
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1878
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2226
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2356
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2595
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2769
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..3126
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3606
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            3607..3727
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            3728..3882
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..4056
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4057..4179
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4180..4461
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4462..4515
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4516..4653
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4758
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            4759..5029
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
     exon            5030..8556
                     /gene="Scn2a"
                     /gene_synonym="NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN;
                     Scn2a1; Scn2a2; ScpII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccctgtcttt gcttttcctc tttagtaagg atattattgc acatgtgaga tttttacttc
       61 ctactgtagg ctgaattcta gaaacctgca ctggagactg ctacattcaa cacgcacatg
      121 gattctgtgc tatgattacc attcctttta tttcagcact ttcttacgcg aggagctaaa
      181 cagtgactaa agaagcagga tgaaaagatg gcacggtcag tgctggtacc tccaggacct
      241 gacagcttcc gcttctttac cagggaatcc cttgctgcta tcgaacagcg cattgcagaa
      301 gagaaagcta agagacccaa acaggaacgc aaggacgaag acgatgaaaa tggcccaaag
      361 ccaaatagtg acttggaagc aggaaaatcc cttcctttta tttatggaga cattcctcca
      421 gagatggtgt cagaaccact ggaggacctg gacccctact atatcaataa gaaaaccttt
      481 atagtattga ataaggggaa agcaatctcg cggttcagcg ccacctctgc cctgtacatt
      541 ttaactccct tcaaccccat tagaaaatta gctattaaga ttttggtaca ttctttattc
      601 aatgtgctta tcatgtgcac aattcttacc aactgtgtgt ttatgaccat gagtaaccct
      661 ccagactgga caaagaatgt ggagtatact tttacaggaa tttatacttt tgaatcactt
      721 attaaaatcc ttgcaagggg cttttgtcta gaagatttca catttctacg ggatccctgg
      781 aattggttgg atttcacagt cattactttt gcgtatgtaa cagaatttgt aaacctaggc
      841 aatgtttcag ctcttcgaac tttcagagtc ttgagagctt tgaaaactat ttctgtaatt
      901 ccaggcctga agaccatcgt gggggccctg atccagtcag tgaagaagct ctctgacgtc
      961 atgatcctca ccgtgttctg tctcagtgtc tttgctctaa tcgggctgca gctcttcatg
     1021 ggcaacctga ggaataaatg cttgcagtgg cccccagaca attctacctt tgaaataaat
     1081 atcacttcct tctttaataa ctcattggat tggaatggta ctgccttcaa taggacggtg
     1141 aacatgttta actgggacga atatattgaa gataaaagtc acttttattt tttggaagga
     1201 caaaacgatg ctctgctttg tgggaacagc tcggatgctg gacagtgtcc agaaggatac
     1261 atctgtgtga aggctgggag aaaccccaac tacggctaca caagttttga caccttcagc
     1321 tgggccttct tgtccttatt tcgtctcatg actcaagact tctgggaaaa cctttatcag
     1381 ctgaccttgc gtgctgccgg gaaaacatac atgatatttt ttgtgctggt cattttcttg
     1441 ggctcattct acctgataaa tttgatcctg gctgtggtgg ccatggccta cgaggaacag
     1501 aaccaggcca cactggagga ggctgaacag aaggaggcag agtttcagca gatgctggag
     1561 caactgaaga agcagcagga ggaagctcag gcagcagctg cagcagcgtc cgcagaatcc
     1621 agagacttca gcggggcagg cgggataggt gttttctcgg agagttcgtc agtagcctca
     1681 aagttaagtt ccaaaagtga aaaggagttg aaaaacagaa gaaagaaaaa gaaacagaaa
     1741 gaacaggctg gggaggaaga gaaggaagac gcggtgcgga aatctgcctc tgaggacagc
     1801 atacgaaaga aaggcttccg gttttccctg gaaggaagta gattgaccta cgagaagaga
     1861 ttttcctctc cgcaccagtc tctcttgagc atccgaggct ccctattttc tccaagacgc
     1921 aacagtagag caagcctttt cagcttcaaa ggtcgagtga aggatattgg ttccgaaaat
     1981 gactttgcag acgatgaaca cagcacattt gaggacaacg acagcagaag agactctcta
     2041 tttgtaccac acagacatgg agaaaggcgt cctagcaatg tcagccaggc cagccgtgcc
     2101 tcccggggga tacccactct acccatgaat gggaagatgc acagtgcagt ggactgcaac
     2161 ggtgtggtgt ccctggttgg aggcccctct gcgctcacat cgcctgtggg gcagctgctg
     2221 ccggagggca caactactga gacagaaata aggaagagga gatccagttc ttaccacgtc
     2281 tctatggact tgttggaaga cccttcaagg caaagggcaa tgagtatggc cagtattttg
     2341 acgaacacta tggaagaact tgaagaatcc agacagaaat gcccaccctg ctggtataaa
     2401 tttgctaata tgtgcctgat ttgggactgt tgtaagccat ggctaaaggt aaaacacgtt
     2461 gtcaatctgg tagtgatgga tccatttgtt gacctggcca tcaccatctg cattgtgtta
     2521 aatacgctct tcatggccat ggagcactac cccatgactg agcagttcag cagtgtgctg
     2581 tctgttggga atttggtctt cactgggatc ttcacggcag aaatgtttct caagataata
     2641 gccatggatc catattatta cttccaagaa ggctggaata tctttgatgg ctttatagtg
     2701 agccttagtt taatggaact cggcttggcg aatgtggaag gattgtcagt tctccgatca
     2761 ttccggctgc ttagagtctt caagttggca aagtcctggc ccacactgaa catgctcatt
     2821 aagatcatcg gcaactcggt gggtgcactg ggcaacctga ccctggtgct ggccatcatc
     2881 gtcttcattt ttgctgtggt cggcatgcag ctgtttggaa agagctacaa ggagtgtgtc
     2941 tgtaagattt ccaatgattg tgagctcccg cgctggcaca tgcacgactt cttccactcc
     3001 ttcctgatcg tgtttcgagt gctgtgtggg gagtggatag agaccatgtg ggactgcatg
     3061 gaggtcgcgg gccagaccat gtgccttact gtcttcatga tggtcatggt gattgggaac
     3121 cttgttgtcc tgaacctctt cttggccttg ctcctcagtt ctttcagctc agacaacctg
     3181 gctgccacag atgacgataa cgaaatgaac aacctccaga tagccgtggg aaggatgcag
     3241 aagggaattg attttgttaa aaggaagata cgtgaattca ttcagaaagc ctttgtcaga
     3301 aagcagaaag ctttagatga aatcaaaccg ctggaagatc tgaataacaa gaaagacagt
     3361 tgtatctcca accacacgac catagaaata ggcaaggacc tcaattacct caaagatgga
     3421 aacgggacga ccagtggcat aggcagcagt gtggagaagt atgtggtaga tgagagtgat
     3481 tacatgtcgt tcataaacaa ccccagcctc accgtgactg tgcccatcgc ccttggagag
     3541 tctgactttg aaaacttaaa cacggaagaa ttcagtagtg agtcagatat ggaagaaagc
     3601 aaggagaaat tgaatgcaac tagctcatct gagggcagta cggttgatat aggcgctccg
     3661 gcagagggag agcagccaga ggccgaaccg gaagaatcgc ttgaaccgga agcgtgtttc
     3721 acagaagact gtgtgagaaa gttcaagtgt tgtcagataa gcatagaaga gggcaaaggc
     3781 aagctctggt ggaacttgag gaaaacgtgc tacaagatag tagagcataa ctggtttgaa
     3841 accttcatcg tctttatgat tctgctcagc agtggtgctc tggcctttga agacatttat
     3901 attgagcagc gaaagaccat caagaccatg ctggagtatg ctgacaaggt tttcacttat
     3961 atcttcatcc tggagatgct tctaaagtgg gtggcctatg gtttccaaat gtatttcacc
     4021 aatgcctggt gctggctgga cttcctgatc gttgatgtct ccttggttag cttaactgca
     4081 aatgccttgg gctattcgga acttggtgcc atcaagtccc tccgaacgtt aagagctctg
     4141 agacctctac gagccttatc ccgatttgaa ggaatgaggg ttgttgtaaa cgctctctta
     4201 ggcgccatcc cctccataat gaacgtactt ctggtctgcc tgatcttttg gctaatattc
     4261 agtatcatgg gggtgaatct ctttgctggc aagttctacc attgcatcaa ctacaccacg
     4321 ggggaaatgt ttgatgtgag cgtggtcaac aactacagtg aatgccaagc tctcattgag
     4381 agcaatcaga cggccaggtg gaagaacgtg aaggtcaact tcgataacgt gggactcgga
     4441 tacctttctc tgctccaagt agccaccttt aaaggatgga tggatatcat gtatgcagct
     4501 gttgactcaa gaaatgtaga actgcagccc aaatacgaag acaatctcta catgtacctt
     4561 tactttgtca tcttcatcat cttcggctca ttcttcaccc tgaacctgtt cattggtgtc
     4621 atcatagaca acttcaacca gcagaagaag aagtttggag gtcaagacat ctttatgaca
     4681 gaagaacaga agaaatacta caatgcaatg aagaagctcg gctccaagaa accgcagaag
     4741 cccattcctc ggcctgcaaa caaatttcaa gggatggtct ttgattttgt aaccaaacaa
     4801 gtctttgaca tcagcatcat gatcctcatc tgcctcaaca tggtgaccat gatggtggaa
     4861 acggacgacc agagtcagga gatgaccaac atcctgtact ggatcaacct ggtgttcatt
     4921 gtcctgttca cgggcgagtg tgtgctgaag ctcatctccc tccgccatta ttatttcacc
     4981 atcggctgga atatttttga ttttgtggta gtcatcctct ccattgtagg aatgtttctc
     5041 gcggagctga tagagaagta tttcgtgtcc cctaccctgt tccgagtcat ccgcctggcc
     5101 aggattggac gaatcctacg cctgatcaaa ggcgccaagg ggatccgcac tctgctcttt
     5161 gctctgatga tgtcccttcc tgcgctgttc aacatcggcc tcctgctttt cctggtcatg
     5221 ttcatctacg ccatctttgg gatgtccaac tttgcctacg ttaagaggga agttggaatt
     5281 gatgacatgt tcaactttga gacttttggc aacagcatga tctgcctgtt ccaaatcacc
     5341 acctctgccg gctgggacgg actgctggcc cctatactaa atagcggacc tcctgactgt
     5401 gaccctgaaa aagatcaccc tggaagctcg gtgaaggggg actgtgggaa cccatctgtg
     5461 gggattttct tttttgtcag ctacatcatc atatccttcc tggtggtggt gaacatgtac
     5521 atcgctgtca tcctggagaa cttcagcgtc gccaccgaag aaagtgcaga gcctctgagt
     5581 gaggacgact ttgagatgtt ctacgaggtc tgggagaagt tcgaccctga cgccactcag
     5641 ttcatagagt tctgcaagct ttctgacttt gcagctgccc tggatcctcc cctcctcatc
     5701 gcaaagccaa acaaagtcca gctcattgcc atggacctgc ccatggtgag tggagaccgc
     5761 atccactgcc tggacatctt gtttgctttt acaaagcggg tcctgggcga gagtggagag
     5821 atggacgctc ttcgaatcca gatggaagaa aggttcatgg cttccaaccc ctccaaggtc
     5881 tcttatgagc ccattaccac caccctgaaa cggaaacaag aggaggtgtc tgctattgtc
     5941 atccagcgag catacaggcg gtatctcctg aagcagaaag ttaagaaagt ttcgtctatc
     6001 tataaaaaag acaaaggtaa agaagacgag ggaacgccca tcaaagaaga catcatcaca
     6061 gataaactga acgagaattc aactccagag aagactgatg tgacaccttc cacaacttct
     6121 ccaccttcct acgatagcgt gacgaaaccg gagaaggaaa aatttgaaaa ggacaaatca
     6181 gaaaaagagg acaaagggaa agatatcagg gaaagtaaaa agtaaaaaaa taaaataata
     6241 ataataataa cgaaaattaa aaattaaaaa aaaaagaata ttccatttcg tgatcaactg
     6301 tttacagcct gtgttggtga tgtatctgtg actccctcag gaggtccatg ccaaactgac
     6361 tgtttctcta agtgtatact taaggtcagt gcctataaca agacaggacc tcgggtcagc
     6421 acactgggat tcaccaaacc agacaaagag caccgacggg aggcttctat gttcacaacc
     6481 agctgacact gctgaagagc ggagagcaat ggctactcag actagaagaa catggaaacg
     6541 gggaggaaag ttaaattttt atgtaaattt aacatgtgac acttgatagc ggtaactgtc
     6601 accactgttt gtgttttaac tgccacacct gccatatttt tacaaaatgt gtgctgtgac
     6661 tttatcactt tttttttttt tgattcacgg gttgtttact cttatatgtg actatttttg
     6721 taaacgggtt tatgttcggg gagagggatt acggagggga ttcgacgttt ctctatgtat
     6781 aacgagatct attttcaacg aaggtaatgc tgcagctctc attcacacac gacgatatcc
     6841 catcacacca ggaaagagct tacttctcat ttcaggatgt ttttagattt tttgaggtgc
     6901 ttaaatagct attcatattt ttaaggtgtt tcatccagag gaattttaat gtgcctgtaa
     6961 atgttccata gaaccacaac catcaaaaag agttgtttta tttttacata gttcattata
     7021 tgtacatgta tatatgtcta tatgtatatg gccgtgtata tacatatata tgtatacaca
     7081 catgcataca tatgcacaca cagaccatca catgctcaga gtcctgacag catgactata
     7141 acattttttt aataagtgtc ctttggtaaa aaaaatatat atatacatat atatatatct
     7201 cctatcagtc ctttctaaga aacccaaact accaaaaacc acccccacgg ccactttctg
     7261 aagttggttc tgcttttttt tccctgcagt attgtttagc catcttctgc tcttggtaag
     7321 gttgacatag tgtatgtcaa ttaaaaaaca acaacagcaa caaagtctgc tttgtaaata
     7381 gtaattttac ccagtggtgc atgtttgagc aaatgaaaat gatttaagca cagtatttac
     7441 tgcatcaaat atgtaccaca gttaagtata gtttgcaagc tttcaacagg cgacatggtg
     7501 tcactgggtc ccttagagtt tgaagctgtc accgctgcat gtctctcttg ctgatgccgc
     7561 tgtatcttaa cccttctgct gttcagaagt ctaacaggga agccatacgt cggtggcgaa
     7621 gtgaagcaaa tcggtccacc aggaactcac tcttcgtgac atgagccatg ggttgcggta
     7681 aacgggaagg agatccttgt tcttggttct cccaaacctt aatggaacag ccggtgagat
     7741 gtctgacagt gtaagctgct tagatctgtt aaaagtatgt gcttcgagtt ttctaaggaa
     7801 atatacatat tgtaaaaaag gggggctcat tttattttat ttcaaagcct tttgtacata
     7861 aaatgggaaa ttaaaagtat cttcaggttg acgtcacagt tctaccgtta gtttctgttc
     7921 cccggcactt tttaatcaag gcacttcgtc acggaagaga taaggcctaa gaggcagtgt
     7981 agggctctgg ctgtcgttgt tggtactgtg gacttgcgcg tatttccact tgaaacaatt
     8041 ctcattccaa gtctaatgtc catttgcttt caatagtgat gccttatcat tgacaagaga
     8101 tttcaagggg agcagatgac actcttggca ttgcctgagt aggatgtcct cacctagtgt
     8161 ttctcctcta ctcatccatc ttttttaatg ttcgtttaca cagatagata gatggataga
     8221 tagaatctta ctcatccaca tggcaccaga gctgtttcag gtatgattcg ggaaccaagc
     8281 gctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc
     8341 tctctgtttt ccacaaaacc agttagtgaa atgatatcgc caattacagc caaacacttt
     8401 gtttcacaga caaccttcaa tgtagattct ttatactaaa gctgttgact tgtagcgtgt
     8461 tcatgaatgc atgcaggaaa atgctattac catacagagc taccggtaag ccacattacg
     8521 atcaagccaa aagaataaag attttttttt tgcttt
//
