LOCUS       NM_001007755            2176 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus selenocysteine lyase (Scly), mRNA.
ACCESSION   NM_001007755 XM_343627
VERSION     NM_001007755.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Seale,L.A., Hashimoto,A.C., Kurokawa,S., Gilman,C.L., Seyedali,A.,
            Bellinger,F.P., Raman,A.V. and Berry,M.J.
  TITLE     Disruption of the selenocysteine lyase-mediated selenium recycling
            pathway leads to metabolic syndrome in mice
  JOURNAL   Mol Cell Biol 32 (20), 4141-4154 (2012)
   PUBMED   22890841
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Omi,R., Kurokawa,S., Mihara,H., Hayashi,H., Goto,M., Miyahara,I.,
            Kurihara,T., Hirotsu,K. and Esaki,N.
  TITLE     Reaction mechanism and molecular basis for selenium/sulfur
            discrimination of selenocysteine lyase
  JOURNAL   J Biol Chem 285 (16), 12133-12139 (2010)
   PUBMED   20164179
  REMARK    GeneRIF: ESI-MS analysis of wild-type and C375A mutant SCL revealed
            that the catalytic reaction proceeds via the formation of an
            enzyme-bound selenopersulfide intermediate on the catalytically
            essential Cys-375 residue.
REFERENCE   3  (bases 1 to 2176)
  AUTHORS   Jafari,C., Panzer,U., Steinmetz,O.M., Zahner,G., Stahl,R.A. and
            Harendza,S.
  TITLE     Enhanced expression of selenocysteine lyase in acute
            glomerulonephritis and its regulation by AP-1
  JOURNAL   Cell Mol Biol Lett 11 (3), 424-437 (2006)
   PUBMED   16874457
  REMARK    GeneRIF: An enhancer element between bp -152 and -298 of the SCL
            5'-regulatory region was identified, with protein binding to an
            AP-1 binding site that may be involved in the regulation of SCL-RNA
            in vivo in an endogenous feedback mechanism.
REFERENCE   4  (bases 1 to 2176)
  AUTHORS   Mihara,H., Kurihara,T., Watanabe,T., Yoshimura,T. and Esaki,N.
  TITLE     cDNA cloning, purification, and characterization of mouse liver
            selenocysteine lyase. Candidate for selenium delivery protein in
            selenoprotein synthesis
  JOURNAL   J Biol Chem 275 (9), 6195-6200 (2000)
   PUBMED   10692412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On Jan 10, 2023 this sequence version replaced NM_001007755.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079358.1, SRR26360186.289524.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               JAXUCZ010000009.1  99337874-99337976
            104-216             JAXUCZ010000009.1  99340117-99340229
            217-317             JAXUCZ010000009.1  99343226-99343326
            318-498             JAXUCZ010000009.1  99345186-99345366
            499-626             JAXUCZ010000009.1  99347528-99347655
            627-791             JAXUCZ010000009.1  99347856-99348020
            792-898             JAXUCZ010000009.1  99349034-99349140
            899-935             JAXUCZ010000009.1  99352745-99352781
            936-1019            JAXUCZ010000009.1  99354352-99354435
            1020-1119           JAXUCZ010000009.1  99354884-99354983
            1120-1195           JAXUCZ010000009.1  99356625-99356700
            1196-2176           JAXUCZ010000009.1  99357507-99358487
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q36"
     gene            1..2176
                     /gene="Scly"
                     /note="selenocysteine lyase"
                     /db_xref="GeneID:363285"
                     /db_xref="RGD:1359514"
     exon            1..103
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1349
                     /gene="Scly"
                     /EC_number="4.4.1.16"
                     /codon_start=1
                     /product="selenocysteine lyase"
                     /protein_id="NP_001007756.1"
                     /db_xref="GeneID:363285"
                     /db_xref="RGD:1359514"
                     /translation="MDVARNGARGSVESPPNRKVYMDYNATTPLEPEVIQAVTEAMKE
                     AWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCF
                     HEQQTLQGRTVDQISPEEGTRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVN
                     GQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLV
                     HTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPRIGALYVRGVGKLTPLYPMLFGGGQ
                     ERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNS
                     RFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSCGI
                     PVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQLEGPV"
     exon            104..216
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            217..317
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            318..498
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            499..626
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            627..791
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            792..898
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            899..935
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1019
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1119
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1195
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..2176
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagtctaag gggtgctcag tcttggggtt accggccagc gatcagggag atggacgtgg
       61 cgcggaatgg cgcgcggggc agtgtggaga gcccgcccaa caggaaggtc tatatggact
      121 acaatgccac cacacctcta gagcccgaag tcatccaggc tgtgacggaa gccatgaaag
      181 aagcctgggg aaaccccagc agctcatatg tagcaggtag gaaggccaag gacattataa
      241 acacagctcg ggcaagcctg gccaagatga tagggggaaa gccccaggac atcatcttca
      301 cttctggggg caccgagtcc aataatttag taatccactc tacggtgaga tgcttccatg
      361 agcagcagac cctgcagggg cgcacggttg accagatcag ccctgaggag gggaccaggc
      421 cccattttat cacttgcacg gtggagcatg actccatccg gctgcccctg gagcacctgg
      481 tggaagatca agtggcagag gtcacttttg tcccagtgtc gaaggtaaat gggcaggtag
      541 aagttgagga tatcctggcc gctgtccgtc ccacaacatg ccttgtgacc atcatgttgg
      601 ccaataacga gactggtgtc atcatgccca tctctgagat cagtcgtcgc attaaagccc
      661 tgaaccagat ccgggctgcc tcggggctac cccgagtcct ggtgcacaca gacgctgctc
      721 aggcactggg gaaaaggcga gtggatgtcg aggacctggg cgtggacttc ctgaccatcg
      781 tggggcataa gttctacggc cccaggatcg gtgctctgta tgtgcgaggg gtcggtaaac
      841 ttacccccct gtaccccatg ctgtttggag gtggacaaga gcggaatttc aggccaggga
      901 ccgagaacac gcccatgatt gctggccttg ggaaggctgc ggatctggtg agtgagaact
      961 gcgagactta tgaggcccac atgagagaca tccgggatta cctggaggag aggctggagg
     1021 ctgaatttgg taagagaatc catttgaaca gccgctttcc aggagtggag aggcttccga
     1081 acacctgcaa cttttccatc caggggtccc agcttcgagg gtacatggtc cttgcacagt
     1141 gccagacact gctggccagc gtgggagctt catgccactc ggatcatgag gaccggccat
     1201 ccccagtgct gctgagctgt ggtattcctg tggatgtggc ccggaacgca gtccggctca
     1261 gtgtgggccg cagtaccact agggctgaag tggacctcat cgtgcaggac ctgaagcagg
     1321 ccgtgaacca gctggaaggc ccggtctaga gctagcagcc actctctccc tcagcggcca
     1381 gctggacttt ggccactcaa tccataggaa accgcttgtg aaagttcctg gcccctaagc
     1441 tgtcccttct caagagctgc actgagatga ctgtcctact atggggtgca aagtgacagg
     1501 gggctgccca ctgcacagta tcccactcta gtgcccatgc ttataagctc aggacagccg
     1561 tgtcacatag gacgtcccct gtcacctccg acaagccaca ggggaatagg aatatcccag
     1621 ttatcaggca gcctgacagg accacccgac cccagaagac actttgatca gaagacaaaa
     1681 cctacgtctt ggtctactgt cacttagctg ctcatgtgag aataggaagt tgatttcgtc
     1741 tccacccatg gcctcccgtg tgcacctaca gacaacaccc ctcccccatg ctgccctgcc
     1801 cttctcctct gccctcagcc tgccactgcc cacctgcctc cctggcctga tggtccccag
     1861 acgaggtctc atgccttgtt cccagaggtc acagctcacc ctgtcttcta cacagggatg
     1921 gctgccggga ggctgctgcc tgatctttgc caaagtggga aatccggtca tttctcactc
     1981 agattcaatt ttaattttat ctgcaaaggg ttctgtcatc ttgaggatgt gtggcacaca
     2041 tcacacccat gtttatatag gaaatgtctt cttggaggac acttttagca cgtgggtctg
     2101 tgggccaaag gatgacgcag catgtggaaa cagttgtcag aagttaaatg aactaatgaa
     2161 agatgcctgc acttgc
//
