LOCUS       NM_198748               2840 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus scinderin (Scin), mRNA.
ACCESSION   NM_198748 XM_216699
VERSION     NM_198748.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wang,J. and Richards,D.A.
  TITLE     The actin binding protein scinderin acts in PC12 cells to tether
            dense-core vesicles prior to secretion
  JOURNAL   Mol Cell Neurosci 85, 12-18 (2017)
   PUBMED   28823945
  REMARK    GeneRIF: we found evidence for a role for scinderin in the
            tethering of dense-core vesicles prior to secretion.
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Revenu,C., Ubelmann,F., Hurbain,I., El-Marjou,F., Dingli,F.,
            Loew,D., Delacour,D., Gilet,J., Brot-Laroche,E., Rivero,F.,
            Louvard,D. and Robine,S.
  TITLE     A new role for the architecture of microvillar actin bundles in
            apical retention of membrane proteins
  JOURNAL   Mol Biol Cell 23 (2), 324-336 (2012)
   PUBMED   22114352
REFERENCE   3  (bases 1 to 2840)
  AUTHORS   Chumnarnsilpa,S., Lee,W.L., Nag,S., Kannan,B., Larsson,M.,
            Burtnick,L.D. and Robinson,R.C.
  TITLE     The crystal structure of the C-terminus of adseverin reveals the
            actin-binding interface
  JOURNAL   Proc Natl Acad Sci U S A 106 (33), 13719-13724 (2009)
   PUBMED   19666531
REFERENCE   4  (bases 1 to 2840)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   5  (bases 1 to 2840)
  AUTHORS   Noda,Y., Horikawa,S., Katayama,Y. and Sasaki,S.
  TITLE     Identification of a multiprotein 'motor' complex binding to water
            channel aquaporin-2
  JOURNAL   Biochem Biophys Res Commun 330 (4), 1041-1047 (2005)
   PUBMED   15823548
REFERENCE   6  (bases 1 to 2840)
  AUTHORS   Svensson,C., Silverstone,A.E., Lai,Z.W. and Lundberg,K.
  TITLE     Dioxin-induced adseverin expression in the mouse thymus is strictly
            regulated and dependent on the aryl hydrocarbon receptor
  JOURNAL   Biochem Biophys Res Commun 291 (5), 1194-1200 (2002)
   PUBMED   11883943
REFERENCE   7  (bases 1 to 2840)
  AUTHORS   Zunino,R., Li,Q., Rose,S.D., Romero-Benitez,M.M., Lejen,T.,
            Brandan,N.C. and Trifaro,J.M.
  TITLE     Expression of scinderin in megakaryoblastic leukemia cells induces
            differentiation, maturation, and apoptosis with release of
            plateletlike particles and inhibits proliferation and tumorigenesis
  JOURNAL   Blood 98 (7), 2210-2219 (2001)
   PUBMED   11568009
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Sep 16, 2022 this sequence version replaced NM_198748.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY324394.1, SRR26643429.23700.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-263               JAXUCZ010000006.1  62915025-62915287   c
            264-418             JAXUCZ010000006.1  62909075-62909229   c
            419-580             JAXUCZ010000006.1  62906081-62906242   c
            581-730             JAXUCZ010000006.1  62882833-62882982   c
            731-823             JAXUCZ010000006.1  62862399-62862491   c
            824-956             JAXUCZ010000006.1  62860122-62860254   c
            957-1045            JAXUCZ010000006.1  62859552-62859640   c
            1046-1261           JAXUCZ010000006.1  62858449-62858664   c
            1262-1383           JAXUCZ010000006.1  62855779-62855900   c
            1384-1474           JAXUCZ010000006.1  62855255-62855345   c
            1475-1645           JAXUCZ010000006.1  62849705-62849875   c
            1646-1823           JAXUCZ010000006.1  62845633-62845810   c
            1824-1945           JAXUCZ010000006.1  62845280-62845401   c
            1946-2023           JAXUCZ010000006.1  62839857-62839934   c
            2024-2084           JAXUCZ010000006.1  62837919-62837979   c
            2085-2840           JAXUCZ010000006.1  62836641-62837396   c
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q21"
     gene            1..2840
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /note="scinderin"
                     /db_xref="GeneID:298975"
                     /db_xref="RGD:735034"
     exon            1..263
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..2212
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /function="specifically cleaves actin filament to permit
                     vesicle docking during regulated secretion"
                     /note="regulated by pH and Ca2+; adseverin"
                     /codon_start=1
                     /product="scinderin"
                     /protein_id="NP_942043.2"
                     /db_xref="GeneID:298975"
                     /db_xref="RGD:735034"
                     /translation="MAQELQHPEFARAGKQAGLQVWRVEKLELVPVPQGAYGDFYVGD
                     AYLVLHTTKSSRGFSYRLHFWLGKECSQDESTAAAIFTVQMDDYLGGKPVQSRELQGY
                     ESTDFVGYFKGGLKYKAGGVASGLNHVLTNDLTAKRLLHVKGRRVVRATEVPLSWQSF
                     NKGDCFIIDLGTEIYQWCGSSCNKYERLKASQVATGIRDNERKGRSQLIVVEEGSEPP
                     ELMKVLGRKPELPDGDNDDDVIADISNRKMAKLYMVSDASGSMKVTLVAEENPFSMGM
                     LLPEECFILDHGAAKQIFVWKGKNANPQERKTAMKTAEEFLHKMNYSANTQIQVLPEG
                     GETPIFKQFFKDWKDKDQSDGFGKVYITEKVAQIKQIPFDASKLHSSPQMAAQHNMVD
                     DGSGGVEIWRVENSGRVQIDPSSYGEFYGGDCYIILYTYPRGQIIYTWQGADATRDEL
                     TMSAFLTVQLDRSLGGQAVQVRVSQGKEPAHLLSLFKDKPLIIYKNGTSKKGGQAPAP
                     PTRLFQVRRNLASITRIVEVDVDANSLNSNDTFVLKLPRNNGFIWIGKGASQEEEKGA
                     EYVADVLKCKTTRIQEGKEPDEFWNSLGGRGDYQTSPLLETQAEDHPPRLYGCSNKTG
                     RFIIEEVPGEFTQDDLAEDDVMLLDAWEQIFIWIGKDANEVEKKESVKSAKMYLETDP
                     SGRDKRTPIVIIKQGHEPPTFTGWFLGWDSSKW"
     exon            264..418
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            419..580
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            581..730
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            731..823
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            824..956
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1045
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1261
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1383
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1474
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1645
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1823
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1945
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..2023
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2084
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2840
                     /gene="Scin"
                     /gene_synonym="Scind"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaccggctct tccttgggct cagggcgcgg atcaagtatt cggctctgcc agcaacaagg
       61 caccatggct caggagctgc agcaccccga gttcgcgcgc gcaggaaaac aggcggggct
      121 gcaagtgtgg agggtcgaga agctggaact ggtaccggtg ccccagggtg cctatggtga
      181 cttttacgtc ggagacgcct acctggtgtt gcacaccacc aagtcgagca ggggcttctc
      241 ctaccgcctg cacttctggc tgggaaagga gtgttcccag gatgagagca cggcagcggc
      301 catcttcacg gtccagatgg acgactattt gggcggcaag ccggtgcaga gcagagagct
      361 tcaaggctat gagtcgactg attttgtggg ctatttcaaa ggcggtctga agtacaaggc
      421 cggaggtgtg gcttctggac taaaccatgt cctcaccaat gacctgacgg cgaaaaggct
      481 tctgcatgtg aagggtcgga gagtggtcag ggccacagaa gttcccctca gctggcagag
      541 cttcaacaag ggcgactgct tcatcattga ccttggcacc gaaatttacc agtggtgtgg
      601 ttcctcctgc aacaaatatg agcgtctgaa agcaagtcag gtggccactg gcatccggga
      661 caatgagagg aagggaagat ctcaactcat tgtggtggaa gaaggaagtg aaccaccaga
      721 gctcatgaag gttttaggga gaaagcctga acttccagat ggggacaatg atgatgatgt
      781 catagcagac ataagtaaca ggaagatggc gaaactctac atggtttcag atgccagtgg
      841 gtccatgaaa gtgactctgg tggctgaaga aaacccgttc tccatgggca tgttgcttcc
      901 tgaagaatgc ttcattttgg accatggtgc tgcaaaacaa atctttgtgt ggaaaggtaa
      961 aaatgctaac ccgcaggaaa ggaagactgc catgaagaca gctgaggagt ttctacacaa
     1021 aatgaattat tctgccaata ctcaaattca ggtactccca gaaggcggcg agacaccaat
     1081 tttcaaacag ttctttaagg attggaagga taaagaccag agtgacggct ttgggaaggt
     1141 gtacatcaca gagaaagtgg ctcaaataaa gcagattcca tttgatgcat caaaactgca
     1201 cagttctccg cagatggcag cccagcacaa catggtggat gatggctctg gaggggtgga
     1261 gatctggcgt gtggagaaca gtggtagggt ccagattgac ccaagctctt atggtgagtt
     1321 ctatggcggt gactgctaca tcatcctcta cacttatccc agaggacaga tcatctacac
     1381 gtggcaaggg gcagatgcta ccagagacga gctgacgatg tctgcgtttc tgactgttca
     1441 gttggatcgg tctctcggag ggcaggctgt gcaggttcgt gtgtctcaag gcaaagagcc
     1501 tgctcacctg ctgagtttgt tcaaagacaa accactcatt atttataaga acgggacatc
     1561 taagaaagga gggcaggcac cggctccccc tacacgcctc tttcaagtcc ggaggaacct
     1621 ggcatctatc actagaatcg tggaggttga tgtggatgca aattcattga attctaatga
     1681 cacttttgtc ctgaaactgc cacgaaacaa tggcttcatc tggataggaa aaggggctag
     1741 ccaggaggag gagaaaggag cagagtatgt ggctgatgtc ctcaaatgca aaaccacaag
     1801 aattcaagaa ggcaaggaac cagatgaatt ctggaactct cttggaggaa gaggagacta
     1861 ccagacttca ccattgctgg aaacacaggc tgaagatcat cctcctcggc tttatggctg
     1921 ctctaacaaa actggaagat tcattattga agaagttccg ggagagttca cccaggatga
     1981 cttggccgaa gatgatgtca tgctacttga tgcgtgggaa cagatcttca tttggattgg
     2041 caaagatgcc aatgaagttg agaaaaagga atcagtgaag tctgccaaaa tgtaccttga
     2101 gacagaccct tctggaagag acaagaggac accaattgtc atcataaaac aaggccatga
     2161 gccccccaca ttcacaggct ggtttctggg ctgggactct agcaagtggt aaactgagtt
     2221 tcaaaaaacc agcaactatt ctggctgcct caggacagct gcccctcttt gttgttgggg
     2281 atttgtttat ttttgttatt ggcttttgaa gataaatgcc ttctgccaaa tggatacata
     2341 taaatgacta acatgctcct cttcttctct ttatcctctt tttaaaggaa ttgttatatg
     2401 ctatattgaa ataacttaaa gcaaactctt gttttggatc aactttgcaa tctaggggga
     2461 tttctgagga aggaatttta taattcagca actgctagcc agacttgtct ttatacacca
     2521 gagaagaaag ccgcagtgct tctaaacatt tcccctctgg ttattctgtg tgcagtaagg
     2581 tttactttat gtatggctct aacagtgcct tgctgtttcc catctcaggt ccatgtgact
     2641 tggacgctat tcacctccaa gtgtccttat ataagatgaa cttctttagc ttttttagaa
     2701 aactaaaatc acaggccctt cgtgataaaa gacattttat ttctataagt ttaactttat
     2761 acatttgata tcacatgctc aatatcataa agaatatgca ttgaatgttt ttcatcatta
     2821 aaacacatct ttttggaaaa
//
