LOCUS       NM_053642               2185 bp    mRNA    linear   ROD 03-JUL-2024
DEFINITION  Rattus norvegicus sterol-C5-desaturase (Sc5d), mRNA.
ACCESSION   NM_053642
VERSION     NM_053642.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Reyna Gallegos,S., Torres Arrunategui,G., Valenzuela,R.,
            Rincon-Cervera,M.A. and Villanueva Espinoza,M.E.
  TITLE     Adding a purple corn extract in rats supplemented with chia oil
            decreases gene expression of SREBP-1c and retains Delta5 and Delta6
            hepatic desaturase activity, unmodified the hepatic lipid profile
  JOURNAL   Prostaglandins Leukot Essent Fatty Acids 132, 1-7 (2018)
   PUBMED   29735017
  REMARK    GeneRIF: The effect of dietary plant extracts and vegetable oils on
            hepatic SREBP-1c, Delta5 and Delta6 desaturases has been reported.
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Waterham,H.R.
  TITLE     Defects of cholesterol biosynthesis
  JOURNAL   FEBS Lett 580 (23), 5442-5449 (2006)
   PUBMED   16876788
  REMARK    Review article
REFERENCE   3  (bases 1 to 2185)
  AUTHORS   Krakowiak,P.A., Wassif,C.A., Kratz,L., Cozma,D., Kovarova,M.,
            Harris,G., Grinberg,A., Yang,Y., Hunter,A.G., Tsokos,M.,
            Kelley,R.I. and Porter,F.D.
  TITLE     Lathosterolosis: an inborn error of human and murine cholesterol
            synthesis due to lathosterol 5-desaturase deficiency
  JOURNAL   Hum Mol Genet 12 (13), 1631-1641 (2003)
   PUBMED   12812989
REFERENCE   4  (bases 1 to 2185)
  AUTHORS   Saether,T., Tran,T.N., Rootwelt,H., Christophersen,B.O. and
            Haugen,T.B.
  TITLE     Expression and regulation of delta5-desaturase, delta6-desaturase,
            stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase
            2 in rat testis
  JOURNAL   Biol Reprod 69 (1), 117-124 (2003)
   PUBMED   12606372
  REMARK    GeneRIF: testicular distribution of Delta5- and Delta6-desaturase
            and stearoyl-CoA desaturase 1 and 2 shows that all four desaturases
            seem to be localized in the Sertoli cells, with far lower
            expression in germ cells
REFERENCE   5  (bases 1 to 2185)
  AUTHORS   Herman,G.E.
  TITLE     Disorders of cholesterol biosynthesis: prototypic metabolic
            malformation syndromes
  JOURNAL   Hum Mol Genet 12 Spec No 1, R75-R88 (2003)
   PUBMED   12668600
  REMARK    Review article
REFERENCE   6  (bases 1 to 2185)
  AUTHORS   Brunetti-Pierri,N., Corso,G., Rossi,M., Ferrari,P., Balli,F.,
            Rivasi,F., Annunziata,I., Ballabio,A., Russo,A.D., Andria,G. and
            Parenti,G.
  TITLE     Lathosterolosis, a novel multiple-malformation/mental retardation
            syndrome due to deficiency of
            3beta-hydroxysteroid-delta5-desaturase
  JOURNAL   Am J Hum Genet 71 (4), 952-958 (2002)
   PUBMED   12189593
  REMARK    Erratum:[Am J Hum Genet. 2003 Aug;73(2):445]
REFERENCE   7  (bases 1 to 2185)
  AUTHORS   Sugawara,T., Fujimoto,Y. and Ishibashi,T.
  TITLE     Molecular cloning and structural analysis of human sterol C5
            desaturase
  JOURNAL   Biochim Biophys Acta 1533 (3), 277-284 (2001)
   PUBMED   11731337
REFERENCE   8  (bases 1 to 2185)
  AUTHORS   Takakuwa,Y., Nishino,H., Ishibe,Y. and Ishibashi,T.
  TITLE     Properties and kinetics of membrane-bound enzymes when both the
            enzyme and substrate are components of the same microsomal
            membrane. Studies on lathosterol 5-desaturase
  JOURNAL   J Biol Chem 269 (45), 27889-27893 (1994)
   PUBMED   7961720
  REMARK    Erratum:[J Biol Chem 1995 Feb 24;270(8):4180]
REFERENCE   9  (bases 1 to 2185)
  AUTHORS   Honjo,K., Ishibashi,T. and Imai,Y.
  TITLE     Partial purification and characterization of lathosterol
            5-desaturase from rat liver microsomes
  JOURNAL   J Biochem 97 (3), 955-959 (1985)
   PUBMED   4019441
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081704.1.
            
            On Sep 16, 2004 this sequence version replaced NM_053642.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081704.1, AB052846.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..2185
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /note="sterol-C5-desaturase"
                     /db_xref="GeneID:114100"
                     /db_xref="RGD:620775"
     exon            1..32
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
     exon            33..116
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
     exon            117..334
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1024
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /EC_number="1.14.19.20"
                     /note="sterol-C5-desaturase (fungal ERG3,
                     delta-5-desaturase) homolog; sterol-C5-desaturase (fungal
                     ERG3, delta-5-desaturase)-like; sterol-C5-desaturase (ERG3
                     delta-5-desaturase homolog, S. cerevisiae)-like; C-5
                     sterol desaturase; lathosterol 5-desaturase;
                     delta(7)-sterol 5-desaturase; delta(7)-sterol
                     C5(6)-desaturase"
                     /codon_start=1
                     /product="lathosterol oxidase"
                     /protein_id="NP_446094.1"
                     /db_xref="GeneID:114100"
                     /db_xref="RGD:620775"
                     /translation="MDLVLSAADYYFFTPYVYPATWPEDNIIRQTVSLLVVTNLGAYI
                     LYFFCATLSYYFVYDHSLMKHPQFLKNQVSREIMFTVKSLPWISIPTVSLFLLELRGY
                     SKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHHRLLYKHIHKPHHIWKIP
                     TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHDGDFRVPQI
                     FRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKGPHSYVKNMTEKE
                     SNSLAENGCKSKKLCNGEFTKNE"
     exon            335..467
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
     exon            468..568
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
     exon            569..2175
                     /gene="Sc5d"
                     /gene_synonym="Sc5dl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcggctt acggcgctgc tgggcctggc aggttcaacc ctcgaactca tctgatggta
       61 gatcctgaca ccaagtgact gctgacacaa ccattgtttc ttgggtcaca taccagggac
      121 tgcaatggac ctggttctca gtgctgccga ctactatttc ttcactccct atgtgtatcc
      181 agccacatgg cccgaggaca acatcatccg acaaactgtt agtctcctgg ttgtcaccaa
      241 cctgggtgct tacatcctct acttcttctg cgcgaccctt agctattatt ttgtctatga
      301 ccattcatta atgaaacacc cacagttttt aaagaatcaa gtctctcgag agatcatgtt
      361 cactgtcaag tctttgcctt ggatcagcat ccccactgtg tcattgttcc tgctggagct
      421 gaggggttac agcaaactct atgatgacat aggagacttt ccaaatggct ggattcatct
      481 catcatgagc gtgatctcct tcctcttctt caccgacatg ttgatctact ggattcatag
      541 gggcctgcac cacagactgc tctataagca catacataaa cctcatcata tttggaagat
      601 ccccacgccg ttcgcaagtc atgcttttca ccctgtggac ggcttccttc agagtctgcc
      661 ttaccatatc tacccctttg tcttcccgct gcacaaggtg gtctacttag gtttatatgt
      721 cttggttaat gtctggacaa tttctattca tgatggcgac ttccgggttc cccagatctt
      781 caggccgttc attaacgggt cggctcatca cacagaccac cacatgctct ttgactataa
      841 ctatgggcag tacttcacac tgtgggatag aatcggaggc tcttttaaac atccttcctc
      901 tttcgaaggg aaaggaccac atagttacgt gaagaacatg acagaaaaag aatccaacag
      961 ccttgcagaa aatggctgca aaagcaaaaa attatgcaat ggggagttta caaagaacga
     1021 gtagatgatt tcccaatcat taactatttt aaataaaaaa gtaatctgca cacagtcgag
     1081 atacatagcg aataagtggt gccatttgaa tgtcagagtg cttgagtgtt gcgctgagga
     1141 aaggtcctga cagtcaacat cgggaagacc ctggcagcac tgtaatctct tggttaattg
     1201 tccgacattg gtccggggac aacttgtgtc tttccagcca gctggtctgc agcctgtaca
     1261 gccccaacaa catctttcaa tatgatagga agaagctggt ctgtgtccct ttgccttaat
     1321 ccactcatat tcattaagga aaaaataacc attgtgctta acaatactga gattagccag
     1381 gaaagccgac catggagacg ctccagtcag ggagcggctg gctctacagc agggctggga
     1441 cttgcaggac ttcatccacg aaggaacagc agtccaagca ggctgatatg atacatgagt
     1501 ggcaatgtat atgatttact taatggctgg tgtaataaga ttatattttc ttcctataat
     1561 aggcacctta ctctctgggg cattatcaga ttttaataat aaataaatgg taatttatta
     1621 ttcagctctt ccacgagtcc agacaggaga ttatttaatt caagatcttg gtgagtgtca
     1681 ccttttttat ttttttgttt ttcgagacag ggtttctctg tatagccctg gctgtcttgg
     1741 aactctgtag actgggctgg cttctgactc agagctctgc ctgcctctgc tgggattaaa
     1801 ggtgtgcacc acactgccca gcttggtgtg tgtcatttga aacactgttt aaaaaccctg
     1861 cagcaatgaa tggataatgc tagacatgag cttgtggcac ccaggtgaca ccttttttaa
     1921 taaagtggac acactgtact tttaatggat tctcagttac aaagctgact ttcttaatga
     1981 agggaataat cgccaagtac tttagtctac ctgaggaaca ctttcaaacc tcatctccca
     2041 tctcctctct ctccactgcc tccgcagaaa aacatgaaaa aaggtgggtg ttttgagcta
     2101 agtgcatttg cgaggggaga agatgtttgt ataaagatat tctaatggct acttatgcaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaa
//
