LOCUS       NM_053664               3069 bp    mRNA    linear   ROD 31-JUL-2016
DEFINITION  Rattus norvegicus sarcosine dehydrogenase (Sardh), mRNA.
ACCESSION   NM_053664
VERSION     NM_053664.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Tibbetts AS and Appling DR.
  TITLE     Compartmentalization of Mammalian folate-mediated one-carbon
            metabolism
  JOURNAL   Annu. Rev. Nutr. 30, 57-81 (2010)
   PUBMED   20645850
  REMARK    Review article
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Bergeron F, Otto A, Blache P, Day R, Denoroy L, Brandsch R and
            Bataille D.
  TITLE     Molecular cloning and tissue distribution of rat sarcosine
            dehydrogenase
  JOURNAL   Eur. J. Biochem. 257 (3), 556-561 (1998)
   PUBMED   9839943
REFERENCE   3  (bases 1 to 3069)
  AUTHORS   Cook,R.J., Misono,K.S. and Wagner,C.
  TITLE     The amino acid sequences of the flavin-peptides of dimethylglycine
            dehydrogenase and sarcosine dehydrogenase from rat liver
            mitochondria
  JOURNAL   J. Biol. Chem. 260 (24), 12998-13002 (1985)
   PUBMED   4055729
REFERENCE   4  (bases 1 to 3069)
  AUTHORS   Wittwer,A.J. and Wagner,C.
  TITLE     Identification of the folate-binding proteins of rat liver
            mitochondria as dimethylglycine dehydrogenase and sarcosine
            dehydrogenase. Flavoprotein nature and enzymatic properties of the
            purified proteins
  JOURNAL   J. Biol. Chem. 256 (8), 4109-4115 (1981)
   PUBMED   6163778
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF067650.1.
            
            [WARNING] On Jun 29, 2023 this sequence was replaced by
            NM_053664.3.
            
            On Feb 21, 2013 this sequence version replaced NM_053664.1.
            
            Summary: a flavoenzyme involved in the oxidative degradation of
            choline to glycine [RGD, Feb 2006].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF067650.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMD00052296, SAMD00052297
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3069              AF067650.1         36-3104
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..3069
                     /gene="Sardh"
                     /note="sarcosine dehydrogenase"
                     /db_xref="GeneID:114123"
                     /db_xref="RGD:621125"
     CDS             137..2896
                     /gene="Sardh"
                     /EC_number="1.5.8.3"
                     /codon_start=1
                     /product="sarcosine dehydrogenase, mitochondrial"
                     /protein_id="NP_446116.1"
                     /db_xref="GeneID:114123"
                     /db_xref="RGD:621125"
                     /translation="MASLSRVLRVAATCPRGRSARNLGLQPLAREARPTTEKSVPYQC
                     TLKEEAQGASVVPQGPSQPLPSTANVVVIGGGSLGCQTLYHLAKLGVGGVVLLERERL
                     TSGTTWHTAGLLWQLRPSDVEVELLAHTRQVVSHDLEEETGLHTGWIQNGGLFIASNQ
                     QRLDEYKRLMSLGKAYGIESHVLSPAETKDLYPLMNVDDLYGTLYVPRDGTMDPAGTC
                     TTLTRAAVARGAQVIENCAVTGIRVRTDDFGVRRVTAVETQHGSIQTPCVVNCAGVWA
                     SSVGRMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEA
                     NPIFWDEVSDKFAFGLFDLDWDVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHK
                     PLMGEAPELRGFFLGCGFNSAGMMLGGGCGQELAHWIVHGRPEKDMYSYDIRRFHHSL
                     TDHPRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLHEELLGQGCVFQERQGWER
                     PGWFNPQETAQVLDYDYYGAYGHQAHKDYAYSRLLGDEYTFDFPPHHCVIQKECLACR
                     TAAAVFNMSYFGKFYLLGADARKAPDWLFSADVNRPPGSTVYTCMLNQRGGTESDLTV
                     SCLAPGAQASPLAPAFEGDGYYLAVGGAVAQHNWSHINTVLQDQEFRCQLMDCSEDLG
                     MLSIQGPASRDILQDVLDADLSNEAFPFSTHQLVRAAGHLVRAIRLSFVGELGWELHV
                     PQASCLPVYRAVMAAGAKHGLVNAGYRAIDSLSIEKGYRHWHADLRSDDSPLEAGLAF
                     TCKLKTSVPFLGREALEKQRATGLRRRLVCLTVEEEVPMFGLEAIWRNGQVVGHVRRA
                     DFGFTVNKTIAYGYIRDPSGGPVSLDFVKNGDYALERMGVTYAAQVHLKSPFDPDNKR
                     VKGIY"
ORIGIN      
        1 ctgaggtaga ggcactgcct ggacttgcag aacctcgatt gggtccatat catgctgact
       61 tttccttgct aggaatctcc caggagcttc tggaaaatct aggacgaaga ctaaggtaca
      121 ggagccctaa gtccccatgg cttcactgag ccgggtcctc cgtgtagctg ccacctgtcc
      181 tcgagggaga tctgctcgca acctggggct acagccccta gccagggagg ccaggcccac
      241 cactgagaag agtgttccat accagtgcac cctgaaggag gaggcccaag gtgcctctgt
      301 ggtgccccaa ggaccaagcc agcccctgcc tagcacagcc aatgttgtgg tcattggtgg
      361 gggcagtttg ggctgccaga ctctgtacca cttggccaag ctgggtgtag gtggagttgt
      421 gctgctggag agagagcgac tgacctctgg gaccacctgg cacacagcag gcctactgtg
      481 gcagctgcgg cccagcgatg tggaagtaga gcttctggct cacacacggc aagtggtgag
      541 ccatgatctg gaagaggaga ctgggcttca cacaggctgg atccagaatg gtggcctctt
      601 catcgcctcc aaccagcagc gcctggatga atacaagagg ctcatgtcgt tgggcaaggc
      661 ctacggtata gaatcccatg tgctgagccc tgcagagacc aaggatctgt atccgctgat
      721 gaatgtggac gacctctatg ggaccctgta tgtgcctcgg gatggtacca tggacccggc
      781 tggcacctgc actaccctca ccagagcagc agtggctagg ggagctcagg tcattgaaaa
      841 ctgtgcagtg actggcatcc gtgtgcggac agacgacttt ggagtgcggc gggtgacagc
      901 tgtggagact caacatggct ccatccagac accctgcgtg gtcaactgtg caggggtgtg
      961 ggcgagctcc gtgggccgga tggctggagt gaaggtcccg ctggtggcca tgcaccatgc
     1021 ctacgttgtc actgagcgca ttgaggggat ccagaacatg cccaatgtcc gggatcatga
     1081 tgcttctgtc tacctccgcc tgcaggggga tgccttgtct gtgggtggct atgaggccaa
     1141 ccccatcttt tgggatgagg tgtcagacaa atttgccttt ggcctctttg acctggactg
     1201 ggatgtgttc actcagcaca ttgaaggtgc cattaaccgt gtccccgtgt tggagaaaac
     1261 ggggatcaag tccactgtct gtggccctga atccttcaca cccgaccata agcccctgat
     1321 gggggaggct cctgagctcc gaggcttctt ccttggctgt ggcttcaaca gtgcaggcat
     1381 gatgttgggt ggtggctgtg gacaggagct ggcccattgg attgtgcatg ggcgcccaga
     1441 gaaggacatg tacagctatg acatcaggcg tttccaccac tcgctcacgg accacccacg
     1501 ctggatccga gagcgcagcc acgagtccta tgccaagaac tactcggttg tctttcctca
     1561 cgatgagccc ctggcgggcc gcaacatgag gagggaccct ctgcacgagg agctgcttgg
     1621 acaaggctgt gtgtttcaag aacgacaggg ctgggaacgg ccaggatggt tcaaccccca
     1681 agaaacagct caggtcctag actacgacta ctatggggcc tacggacacc aggcacacaa
     1741 ggactacgcc tacagcaggc tgctgggcga tgaatatacc ttcgacttcc caccccacca
     1801 ctgcgtgatc cagaaggaat gtctggcctg cagaacggct gccgccgtgt tcaacatgtc
     1861 ttacttcggg aagttctacc ttctgggggc agacgccagg aaggctccag actggctctt
     1921 ctcagcagat gtcaaccgac ccccaggctc cacggtgtac acatgcatgc tgaaccaacg
     1981 gggaggcacc gagagcgacc tgactgttag ctgcctggcc cctggtgccc aggccagtcc
     2041 cctggcccct gcctttgaag gggatggcta ctacctagcc gtaggtgggg ctgtggccca
     2101 acataactgg tcccacataa acacggtgct gcaggatcag gagttccggt gccagcttat
     2161 ggattgttct gaagatctgg gcatgctcag tatccagggc ccagccagcc gtgacattct
     2221 ccaggatgtg cttgatgctg acctgagcaa tgaagccttc cccttctcca cccaccagct
     2281 ggtgagagct gctggacacc tggtccgagc catccggcta tcctttgtcg gggagctggg
     2341 atgggagctg catgtgcccc aagcgtcctg cttgcctgtg taccgggctg tgatggctgc
     2401 aggggcgaag cacggcctag tcaatgcagg ctaccgtgcc atcgactccc tgagcattga
     2461 gaaaggctat cggcactggc atgcagacct gaggtccgat gacagccccc tggaggcagg
     2521 gttggccttc acctgcaagc tcaagacctc agtgcccttc ctaggccgag aggccctgga
     2581 gaagcagcgt gccacaggcc tccgacgacg cctggtgtgc ctcactgtgg aagaggaggt
     2641 tcctatgttc ggcctagagg ccatctggag aaatggccaa gtggtgggcc atgtccggag
     2701 agctgacttt ggattcaccg tgaacaagac catcgcctat ggctacatcc gagaccccag
     2761 tggagggcca gtctctctgg actttgtgaa gaatggggac tatgccttgg agagaatggg
     2821 ggtcacctat gctgcccagg ttcacctgaa gtctcccttt gatcctgaca acaagcgggt
     2881 gaagggaatc tattgatggg ctgcagggtg gaggctacag ctgctcaggc ccctggaaac
     2941 tgccacactt cccatacggc ctgtggactc ctatactgta caaacagccc tgtgtcctga
     3001 gacccaagat aagggcaaaa cacagacttc ccaccctgag cctccatctg gtcctgctaa
     3061 ctgccacag
//
