LOCUS       NP_446116                919 aa            linear   ROD 31-JUL-2016
DEFINITION  sarcosine dehydrogenase, mitochondrial [Rattus norvegicus].
ACCESSION   NP_446116
VERSION     NP_446116.1
DBSOURCE    REFSEQ: accession NM_053664.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 919)
  AUTHORS   Tibbetts AS and Appling DR.
  TITLE     Compartmentalization of Mammalian folate-mediated one-carbon
            metabolism
  JOURNAL   Annu. Rev. Nutr. 30, 57-81 (2010)
   PUBMED   20645850
  REMARK    Review article
REFERENCE   2  (residues 1 to 919)
  AUTHORS   Bergeron F, Otto A, Blache P, Day R, Denoroy L, Brandsch R and
            Bataille D.
  TITLE     Molecular cloning and tissue distribution of rat sarcosine
            dehydrogenase
  JOURNAL   Eur. J. Biochem. 257 (3), 556-561 (1998)
   PUBMED   9839943
REFERENCE   3  (residues 1 to 919)
  AUTHORS   Cook,R.J., Misono,K.S. and Wagner,C.
  TITLE     The amino acid sequences of the flavin-peptides of dimethylglycine
            dehydrogenase and sarcosine dehydrogenase from rat liver
            mitochondria
  JOURNAL   J. Biol. Chem. 260 (24), 12998-13002 (1985)
   PUBMED   4055729
REFERENCE   4  (residues 1 to 919)
  AUTHORS   Wittwer,A.J. and Wagner,C.
  TITLE     Identification of the folate-binding proteins of rat liver
            mitochondria as dimethylglycine dehydrogenase and sarcosine
            dehydrogenase. Flavoprotein nature and enzymatic properties of the
            purified proteins
  JOURNAL   J. Biol. Chem. 256 (8), 4109-4115 (1981)
   PUBMED   6163778
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF067650.1.
            
            [WARNING] On Jun 29, 2023 this sequence was replaced by
            NP_446116.2.
            
            Summary: a flavoenzyme involved in the oxidative degradation of
            choline to glycine [RGD, Feb 2006].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF067650.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMD00052296, SAMD00052297
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     Protein         1..919
                     /product="sarcosine dehydrogenase, mitochondrial"
                     /EC_number="1.5.8.3"
                     /calculated_mol_wt=99086
     transit_peptide 1..22
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Mitochondrion. {ECO:0000255}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
                     /calculated_mol_wt=2373
     mat_peptide     23..919
                     /product="Sarcosine dehydrogenase, mitochondrial"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64380.2)"
                     /calculated_mol_wt=99086
     Site            38
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Region          67..445
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Site            109
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Tele-8alpha-FAD histidine. {ECO:0000250};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            174
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            174
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            378
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            392
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Region          431..486
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          481..917
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     Site            535
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            560
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            776
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000250|UniProtKB:Q99LB7};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            778
                     /site_type="phosphorylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphotyrosine. {ECO:0000250|UniProtKB:Q9UL12};
                     propagated from UniProtKB/Swiss-Prot (Q64380.2)"
     Site            803
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            803
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            885
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            885
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            905
                     /site_type="acetylation"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     Site            905
                     /site_type="modified"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-succinyllysine, alternate.
                     {ECO:0000250|UniProtKB:Q99LB7}; propagated from
                     UniProtKB/Swiss-Prot (Q64380.2)"
     CDS             1..919
                     /gene="Sardh"
                     /coded_by="NM_053664.2:137..2896"
                     /db_xref="GeneID:114123"
                     /db_xref="RGD:621125"
ORIGIN      
        1 maslsrvlrv aatcprgrsa rnlglqplar earptteksv pyqctlkeea qgasvvpqgp
       61 sqplpstanv vvigggslgc qtlyhlaklg vggvvllere rltsgttwht agllwqlrps
      121 dvevellaht rqvvshdlee etglhtgwiq ngglfiasnq qrldeykrlm slgkaygies
      181 hvlspaetkd lyplmnvddl ygtlyvprdg tmdpagtctt ltraavarga qviencavtg
      241 irvrtddfgv rrvtavetqh gsiqtpcvvn cagvwassvg rmagvkvplv amhhayvvte
      301 riegiqnmpn vrdhdasvyl rlqgdalsvg gyeanpifwd evsdkfafgl fdldwdvftq
      361 hiegainrvp vlektgikst vcgpesftpd hkplmgeape lrgfflgcgf nsagmmlggg
      421 cgqelahwiv hgrpekdmys ydirrfhhsl tdhprwirer shesyaknys vvfphdepla
      481 grnmrrdplh eellgqgcvf qerqgwerpg wfnpqetaqv ldydyygayg hqahkdyays
      541 rllgdeytfd fpphhcviqk eclacrtaaa vfnmsyfgkf yllgadarka pdwlfsadvn
      601 rppgstvytc mlnqrggtes dltvsclapg aqasplapaf egdgyylavg gavaqhnwsh
      661 intvlqdqef rcqlmdcsed lgmlsiqgpa srdilqdvld adlsneafpf sthqlvraag
      721 hlvrairlsf vgelgwelhv pqasclpvyr avmaagakhg lvnagyraid slsiekgyrh
      781 whadlrsdds pleaglaftc klktsvpflg realekqrat glrrrlvclt veeevpmfgl
      841 eaiwrngqvv ghvrradfgf tvnktiaygy irdpsggpvs ldfvkngdya lermgvtyaa
      901 qvhlkspfdp dnkrvkgiy
//
