LOCUS       NM_001009622            1187 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Rattus norvegicus secretion associated, Ras related GTPase 1B
            (Sar1b), mRNA.
ACCESSION   NM_001009622 XM_213283
VERSION     NM_001009622.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Nakagawa,H., Ishizaki,M., Miyazaki,S., Abe,T., Nishimura,K.,
            Komori,M. and Matsuo,S.
  TITLE     Sar1 translocation onto the ER-membrane for vesicle budding has
            different pathways for promotion and suppression of ER-to-Golgi
            transport mediated through H89-sensitive kinase and ER-resident G
            protein
  JOURNAL   Mol Cell Biochem 366 (1-2), 175-182 (2012)
   PUBMED   22484643
  REMARK    GeneRIF: Sar1 recruitment to the endoplasmic reticulum-membrane is
            sensitive to H89-sensitive kinase activation, indicating the first
            step in transport vesicle formation occurs under kinase regulation
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Siddiqi,S. and Mansbach,C.M. 2nd.
  TITLE     Phosphorylation of Sar1b protein releases liver fatty acid-binding
            protein from multiprotein complex in intestinal cytosol enabling it
            to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron
            transport vesicle
  JOURNAL   J Biol Chem 287 (13), 10178-10188 (2012)
   PUBMED   22303004
  REMARK    GeneRIF: Phosphorylation of Sar1b protein releases liver fatty
            acid-binding protein from multiprotein complex in intestinal
            cytosol enabling it to bind to endoplasmic reticulum (ER) and bud
            the pre-chylomicron transport vesicle.
REFERENCE   3  (bases 1 to 1187)
  AUTHORS   Nakagawa,H., Umadome,H., Miyazaki,S., Tanaka,K., Nishimura,K.,
            Komori,M. and Matsuo,S.
  TITLE     ER-resident Gi2 protein controls sar1 translocation onto the ER
            during budding of transport vesicles
  JOURNAL   J Cell Biochem 112 (9), 2250-2256 (2011)
   PUBMED   21480366
  REMARK    GeneRIF: Sar1 translocation onto the endoplasmic reticulum was
            modulated by endoplasmic reticulum-resident G(i2)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FQ217751.1, FQ219007.1 and DV713786.1.
            
            On Mar 8, 2021 this sequence version replaced NM_001009622.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR754931.1, BC088842.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-879               FQ217751.1         34-912
            880-1184            FQ219007.1         687-991
            1185-1187           DV713786.1         707-709
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..1187
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /note="secretion associated, Ras related GTPase 1B"
                     /db_xref="GeneID:287276"
                     /db_xref="RGD:1305590"
     exon            1..98
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     exon            99..174
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..713
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /EC_number="3.6.5.2"
                     /note="GTP-binding protein SAR1b; SAR1 gene homolog B;
                     SAR1 homolog B"
                     /codon_start=1
                     /product="small COPII coat GTPase SAR1B"
                     /protein_id="NP_001009622.1"
                     /db_xref="GeneID:287276"
                     /db_xref="RGD:1305590"
                     /translation="MSFIFDWIYSGFSSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHM
                     LKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHLQARRVWKNYLPAINGIVFLVDC
                     ADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGK
                     GSVSLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYID"
     exon            175..294
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     exon            295..360
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     exon            361..464
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     exon            465..596
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
     exon            597..1187
                     /gene="Sar1b"
                     /gene_synonym="SARA1B; Sara2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggagagct ggggccctgg cagccatagc gctttgcaca gccggctcag agcgtgtagg
       61 acgggcacgg agcagggttg cgttctctgg agtcgcagac cctgtaagat ctggatatgt
      121 ccttcatatt tgactggatt tacagtggtt tcagcagtgt gctgcagttt ctaggattgt
      181 ataaaaaaac tggtaaactg gtattccttg gattggataa tgccgggaaa acaacgttgc
      241 tacacatgct gaaagatgac aggcttggcc agcacgtccc aacactacat cccacttcag
      301 aggagctcac tattgctggc atgacgttta caacttttga tctgggtggg catttgcaag
      361 cccgaagagt atggaaaaac taccttcctg ctatcaatgg cattgtattt cttgtggatt
      421 gtgcagacca tgaaaggctg ttagaatcaa aagaagaact tgattcacta atgacagatg
      481 aaaccattgc caacgtgcct atcctaattc ttggaaacaa gatcgacagg cctgaagcca
      541 taagtgaaga gaggttacga gagatgtttg gtttatatgg tcagacaaca ggaaagggca
      601 gtgtgtcact gaaggagctg aatgcccggc ctctggaagt gttcatgtgc agtgtgctaa
      661 aaaggcaggg ctacggggaa ggcttccgct ggatggcgca gtacatcgac tagcagcggc
      721 cggcccacac ttccagccct cagacccatc cccaacatgc ataacttgaa ttcaatagac
      781 ttttgttggt ttcagaatag gtgtttttag attattaata tatctacctg agtgggaatt
      841 gcaaactgat tcaagtttga atatcacaat gttagctttc taattccaca cagatagttt
      901 ttacagtttc taaccccaca tcaacccatc accgtttata aagaccagct tcccatcggt
      961 acatttgaag cactttttaa cgatgtgaga ctacaaatac aaaccatatt taaaagctca
     1021 tcatgtgaaa cttttatgta ctttttggaa atagtttttc aatttagatt gtctgcctat
     1081 cttgtgtaca gttaaaatcc gcttatcagt gattgcatga tgctttactg ttttcaagac
     1141 tttttcttat gcaaacgtca tgcaataaaa caaactccaa cgtttgg
//
