LOCUS       NM_001007739            2653 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Rattus norvegicus secretion associated, Ras related GTPase 1A
            (Sar1a), mRNA.
ACCESSION   NM_001007739 XM_342136
VERSION     NM_001007739.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Chen,X.W., Wang,H., Bajaj,K., Zhang,P., Meng,Z.X., Ma,D., Bai,Y.,
            Liu,H.H., Adams,E., Baines,A., Yu,G., Sartor,M.A., Zhang,B., Yi,Z.,
            Lin,J., Young,S.G., Schekman,R. and Ginsburg,D.
  TITLE     SEC24A deficiency lowers plasma cholesterol through reduced PCSK9
            secretion
  JOURNAL   Elife 2, e00444 (2013)
   PUBMED   23580231
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Nakagawa,H., Ishizaki,M., Miyazaki,S., Abe,T., Nishimura,K.,
            Komori,M. and Matsuo,S.
  TITLE     Sar1 translocation onto the ER-membrane for vesicle budding has
            different pathways for promotion and suppression of ER-to-Golgi
            transport mediated through H89-sensitive kinase and ER-resident G
            protein
  JOURNAL   Mol Cell Biochem 366 (1-2), 175-182 (2012)
   PUBMED   22484643
  REMARK    GeneRIF: Sar1 recruitment to the endoplasmic reticulum-membrane is
            sensitive to H89-sensitive kinase activation, indicating the first
            step in transport vesicle formation occurs under kinase regulation
REFERENCE   3  (bases 1 to 2653)
  AUTHORS   Nakagawa,H., Miyazaki,S., Abe,T., Umadome,H., Tanaka,K.,
            Nishimura,K., Komori,M. and Matsuo,S.
  TITLE     H89 sensitive kinase regulates the translocation of Sar1 onto the
            ER membrane through phosphorylation of ER-coupled beta-tubulin
  JOURNAL   Int J Biochem Cell Biol 43 (3), 423-430 (2011)
   PUBMED   21111843
  REMARK    GeneRIF: beta-tubulin is one of downstream effectors of
            H89-sensitive kinase and suppression of endoplasmic reticulum
            (ER)-coupled beta-tubulin phosphorylation decreases Sar1
            translocation onto the ER.
REFERENCE   4  (bases 1 to 2653)
  AUTHORS   Bhalla-Gehi,R., Penuela,S., Churko,J.M., Shao,Q. and Laird,D.W.
  TITLE     Pannexin1 and pannexin3 delivery, cell surface dynamics, and
            cytoskeletal interactions
  JOURNAL   J Biol Chem 285 (12), 9147-9160 (2010)
   PUBMED   20086016
REFERENCE   5  (bases 1 to 2653)
  AUTHORS   Merte,J., Jensen,D., Wright,K., Sarsfield,S., Wang,Y., Schekman,R.
            and Ginty,D.D.
  TITLE     Sec24b selectively sorts Vangl2 to regulate planar cell polarity
            during neural tube closure
  JOURNAL   Nat Cell Biol 12 (1), 41-6 (2010)
   PUBMED   19966784
REFERENCE   6  (bases 1 to 2653)
  AUTHORS   Mancias,J.D. and Goldberg,J.
  TITLE     Structural basis of cargo membrane protein discrimination by the
            human COPII coat machinery
  JOURNAL   EMBO J 27 (21), 2918-2928 (2008)
   PUBMED   18843296
REFERENCE   7  (bases 1 to 2653)
  AUTHORS   Mancias,J.D. and Goldberg,J.
  TITLE     The transport signal on Sec22 for packaging into COPII-coated
            vesicles is a conformational epitope
  JOURNAL   Mol Cell 26 (3), 403-414 (2007)
   PUBMED   17499046
REFERENCE   8  (bases 1 to 2653)
  AUTHORS   Nori,A., Bortoloso,E., Frasson,F., Valle,G. and Volpe,P.
  TITLE     Vesicle budding from endoplasmic reticulum is involved in
            calsequestrin routing to sarcoplasmic reticulum of skeletal muscles
  JOURNAL   Biochem J 379 (Pt 2), 505-512 (2004)
   PUBMED   14728599
REFERENCE   9  (bases 1 to 2653)
  AUTHORS   Shen,K.A., Hammond,C.M. and Moore,H.P.
  TITLE     Molecular analysis of SAR1-related cDNAs from a mouse pituitary
            cell line
  JOURNAL   FEBS Lett 335 (3), 380-385 (1993)
   PUBMED   8262187
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079228.1.
            
            On Nov 29, 2004 this sequence version replaced XM_342136.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079228.1, EV774872.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q11"
     gene            1..2653
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /note="secretion associated, Ras related GTPase 1A"
                     /db_xref="GeneID:361842"
                     /db_xref="RGD:1359540"
     exon            1..123
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     exon            124..197
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..736
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /EC_number="3.6.5.2"
                     /note="SAR1 gene homolog A; SAR1a gene homolog 1; SAR1
                     homolog A"
                     /codon_start=1
                     /product="GTP-binding protein SAR1a"
                     /protein_id="NP_001007740.1"
                     /db_xref="GeneID:361842"
                     /db_xref="RGD:1359540"
                     /translation="MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHM
                     LKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDC
                     ADHSRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGK
                     GNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID"
     exon            198..317
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     exon            318..383
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..487
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     exon            488..619
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..2627
                     /gene="Sar1a"
                     /gene_synonym="Sara1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagtctca gggaggggcg tgacgtacat ccggcgcgta gctggcggtc ccgggtactg
       61 ctggtctgtg tgctctgagg gagggtcgcg ccgccagcca ccgccggagg agcccctcgg
      121 gccgttgtaa gcatcaataa tgtctttcat ctttgagtgg atctacaatg gcttcagcag
      181 tgtgcttcag ttcctaggac tctacaagaa atctggaaaa cttgtattct taggtttgga
      241 caatgcgggc aaaaccactc ttcttcacat gctgaaagat gacagactag gccagcacgt
      301 accaacactc catcccactt cagaagaact gacgattgct ggaatgactt tcaccacttt
      361 tgatcttggt gggcacgagc aagcacgtcg ggtttggaaa aattacctcc cagcaattaa
      421 tggaatagtt tttctggtgg actgtgcaga tcattctcgt ctcatggaat ccaaagttga
      481 acttaatgct ttaatgactg atgagacaat atccaacgtg ccaatcctta tcttgggaaa
      541 caaaattgac aggacagatg caatcagtga agaaaaactc cgtgaaatat ttgggctcta
      601 tgggcaaacc acaggaaagg ggaatgtgac tctgaaggag ctgaacgccc gccccatgga
      661 agtgttcatg tgcagtgtgc tcaagaggca aggctacggc gagggtttcc gctggctttc
      721 ccagtatatt gactgatgtt tggacagtga aaataaagag ttttacttct ggactgatcc
      781 tattcacagc ttcctcatgg acttttctaa tagaacaagg aaagctctcc aaccatgtat
      841 ggcattgaga agccacgcat ctgtctacgc tcattgccca gtggtgacat gtgctcttct
      901 ccacactgcc tgcggctgct gccgggcagt gtgccgggac ggggctggca gcatttgcca
      961 gggaaagctg ggtgccatca tgggacaccc gagaagaaaa acatgtctca ccactggttg
     1021 atttcaaaag aaagtgattc tattttttaa agaaagtgtt gttaatgtaa ttggtatccc
     1081 ttctctaact ttttgagttc acaatttact tggtccagat tttctattct tttttttttt
     1141 ttaaactagt aaatgaaatt tagatatttc ataaaattat gaacagatag cgcgatggag
     1201 gccagagctt cgctgtgtga ctgcctgttg aaggtggtgc ctgagcacag gcggggagag
     1261 gacagccttg gcttacccgc catggtctcc accatcatct ggagtgggta aagtgtcatg
     1321 atcacgttgt caacttcatt tgcagttacc ggtttcagca ggctttgctt ttatactgtt
     1381 gtggaatttc atctctctgt gtagcacctt ccttttttca ctgtagatat aagaagtgac
     1441 ttttgcctca tgagggaatt aaggacatct ctcatgtaga ccctgtgctg ggggtgtgac
     1501 tctgatgctt ttgaacatac gctagaggga cggttctgaa caggatgcag ggcaaaatca
     1561 gcagcttaag gctctagatt ctgtgagatg ctacccatct gatctggaag caatgaggga
     1621 acctaggaaa aactctgagg ggctcttgtg ttttcttcag aaataacaga gcaaacccag
     1681 gtggcctggc agcagcgagt gctagtgtgg gggcagcagc ggctgtgagg accatgaaca
     1741 ggacttttat atatagcatt tgcttgtctt gctgctgtgt gtgaggttga attttcctga
     1801 tgttaacttt gtattgtacc cgtggagtgg atgagacggt ctgcagacag gagggtgtct
     1861 tgattctgat ttcctctaat gcaccgtgtg agtacgttcg taagtggaaa ttatgctgct
     1921 gctgttttct gtcagactca tgtccagcgt gagggtagag aaggtaggag cacaccgggg
     1981 agtgacttat cacgttttag ttttgagcac tgagttcatg ggacagtgtt ctgttacact
     2041 gccaggtccc caatgcagag gctcggtggg gtgtctgtct gcaaactgca tgtgttgcct
     2101 tcaccgacgg acagctggaa tgagaactgt cagtgcggag gggcctttgt taaagccttg
     2161 agtgagctgg gtaggtaggg tgtgcccttc attgccttgg ctaaagactg tccacatgtc
     2221 tgtccaggca catgacaggt gtgatggcct cggtgttggg ttcctcattt gtctttctcg
     2281 tttgtcccta attgctcgga aacaacctag aagactggat ggaccctgtc cagggtggct
     2341 ctttgtgaaa gtggtttatt ctagccactg gagaaagaga tggctgaaaa gtggcaccag
     2401 tctgcttcat gctgcacaga gcaccatctt ccataaactg gccaagaggt tgctgcagtg
     2461 agtgcaactc cacctgaggg ccagggcctt gaccaggggc tccaatgaac tgagacttca
     2521 ttctgtttat cagagtgcac gtgtcctgtt tcagcaaacg taaaactgtc atttacaaaa
     2581 gaaggtcagt ctgtatccta agcattttaa taaaaagcta aaatgaaaaa aaaaaaaaaa
     2641 aaaaaaaaaa aaa
//
