LOCUS       NM_001012475            3265 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus relaxin family peptide receptor 2 (Rxfp2), mRNA.
ACCESSION   NM_001012475 XM_344073
VERSION     NM_001012475.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Chen,Y.C., Chang,Y.C., Chang,H.A., Lin,Y.S., Tsao,C.W., Shen,M.R.
            and Chiu,W.T.
  TITLE     Differential Ca2+ mobilization and mast cell degranulation by
            FcepsilonRI- and GPCR-mediated signaling
  JOURNAL   Cell Calcium 67, 31-39 (2017)
   PUBMED   29029788
  REMARK    GeneRIF: Differential Ca(2+) mobilization and mast cell
            degranulation by FcepsilonRI- and GPCR-mediated signaling has been
            reported.
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Dehghan,F., Muniandy,S., Yusof,A. and Salleh,N.
  TITLE     Testosterone reduces knee passive range of motion and expression of
            relaxin receptor isoforms via 5alpha-dihydrotestosterone and
            androgen receptor binding
  JOURNAL   Int J Mol Sci 15 (3), 4619-4634 (2014)
   PUBMED   24642882
  REMARK    GeneRIF: Data indicate that knee passive range of motion (ROM),
            relaxin receptor isoforms Rxfp1 and Rxfp2 expression were
            significantly reduced following treatment with testosterone, and
            androgen antagonist flutamide antagonized the testosterone effect.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3265)
  AUTHORS   Dehghan,F., Muniandy,S., Yusof,A. and Salleh,N.
  TITLE     Sex-steroid regulation of relaxin receptor isoforms (RXFP1 & RXFP2)
            expression in the patellar tendon and lateral collateral ligament
            of female WKY rats
  JOURNAL   Int J Med Sci 11 (2), 180-191 (2014)
   PUBMED   24465164
  REMARK    GeneRIF: RXFP1 & RXFP2 up-regulation by progesterone and estrogen
            could provide the basis for the reported increase in knee laxity
            while down-regulation of these receptor isoforms by testosterone
            could explain low incidence of non-contact knee injury in male.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3265)
  AUTHORS   Feugang,J.M., Rodriguez-Munoz,J.C., Willard,S.T., Bathgate,R.A. and
            Ryan,P.L.
  TITLE     Examination of relaxin and its receptors expression in pig gametes
            and embryos
  JOURNAL   Reprod Biol Endocrinol 9, 10 (2011)
   PUBMED   21251292
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3265)
  AUTHORS   Zhang,L., Zheng,X.M., Hubert,J., Zheng,H., Yang,Z.W. and Li,S.W.
  TITLE     Prenatal exposure to diaethylstilbestrol in the rat inhibits
            transabdominal testicular descent with involvement of the
            INSL3/LGR8 system and HOXA10
  JOURNAL   Chin Med J (Engl) 122 (8), 967-971 (2009)
   PUBMED   19493424
  REMARK    GeneRIF: Prenatal exposure to diaethylstilbestrol can inhibit
            transabdominal testicular descent in a system that does not involve
            LGR8.
REFERENCE   6  (bases 1 to 3265)
  AUTHORS   Bennett,R.G., Mahan,K.J., Gentry-Nielsen,M.J. and Tuma,D.J.
  TITLE     Relaxin receptor expression in hepatic stellate cells and in
            cirrhotic rat liver tissue
  JOURNAL   Ann N Y Acad Sci 1041, 185-189 (2005)
   PUBMED   15956705
  REMARK    GeneRIF: both LGR7 and LGR8 are expressed in activated hepatic
            stellate cells and cirrhotic liver
REFERENCE   7  (bases 1 to 3265)
  AUTHORS   Scott,D.J., Fu,P., Shen,P.J., Gundlach,A., Layfield,S.,
            Riesewijk,A., Tomiyama,H., Hutson,J.M., Tregear,G.W. and
            Bathgate,R.A.
  TITLE     Characterization of the rat INSL3 receptor
  JOURNAL   Ann N Y Acad Sci 1041, 13-16 (2005)
   PUBMED   15956681
  REMARK    GeneRIF: Rat LGR8 bound insulin-like 3 Leydig insulin-like peptide
            (INSL3) with high affinity, clearly demonstrating that it is the
            rat INSL3 receptor
REFERENCE   8  (bases 1 to 3265)
  AUTHORS   Kawamura,K., Kumagai,J., Sudo,S., Chun,S.Y., Pisarska,M.,
            Morita,H., Toppari,J., Fu,P., Wade,J.D., Bathgate,R.A. and
            Hsueh,A.J.
  TITLE     Paracrine regulation of mammalian oocyte maturation and male germ
            cell survival
  JOURNAL   Proc Natl Acad Sci U S A 101 (19), 7323-7328 (2004)
   PUBMED   15123806
REFERENCE   9  (bases 1 to 3265)
  AUTHORS   Gorlov,I.P., Kamat,A., Bogatcheva,N.V., Jones,E., Lamb,D.J.,
            Truong,A., Bishop,C.E., McElreavey,K. and Agoulnik,A.I.
  TITLE     Mutations of the GREAT gene cause cryptorchidism
  JOURNAL   Hum Mol Genet 11 (19), 2309-2318 (2002)
   PUBMED   12217959
REFERENCE   10 (bases 1 to 3265)
  AUTHORS   Boockfor,F.R., Fullbright,G., Bullesbach,E.E. and Schwabe,C.
  TITLE     Relaxin-like factor (RLF) serum concentrations and gubernaculum RLF
            receptor display in relation to pre- and neonatal development of
            rats
  JOURNAL   Reproduction 122 (6), 899-906 (2001)
   PUBMED   11732985
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 3, 2022 this sequence version replaced NM_001012475.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY906861.1, SRR26643288.46641.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               JAXUCZ010000012.1  10022980-10023098   c
            120-266             JAXUCZ010000012.1  10006817-10006963   c
            267-344             JAXUCZ010000012.1  10003787-10003864   c
            345-450             JAXUCZ010000012.1  9998778-9998883     c
            451-522             JAXUCZ010000012.1  9998102-9998173     c
            523-594             JAXUCZ010000012.1  9993206-9993277     c
            595-666             JAXUCZ010000012.1  9992409-9992480     c
            667-738             JAXUCZ010000012.1  9990286-9990357     c
            739-810             JAXUCZ010000012.1  9989241-9989312     c
            811-882             JAXUCZ010000012.1  9987796-9987867     c
            883-954             JAXUCZ010000012.1  9987014-9987085     c
            955-1026            JAXUCZ010000012.1  9982340-9982411     c
            1027-1098           JAXUCZ010000012.1  9982162-9982233     c
            1099-1170           JAXUCZ010000012.1  9978866-9978937     c
            1171-1400           JAXUCZ010000012.1  9976053-9976282     c
            1401-1811           JAXUCZ010000012.1  9974929-9975339     c
            1812-2030           JAXUCZ010000012.1  9971800-9972018     c
            2031-3265           JAXUCZ010000012.1  9961175-9962409     c
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p12"
     gene            1..3265
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /note="relaxin family peptide receptor 2"
                     /db_xref="GeneID:363866"
                     /db_xref="RGD:1309449"
     exon            1..119
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..2290
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /note="INSL3 receptor; relaxin receptor 2; leucine-rich
                     repeat-containing G protein-coupled receptor 8;
                     relaxin/insulin-like family peptide receptor 2"
                     /codon_start=1
                     /product="relaxin receptor 2 precursor"
                     /protein_id="NP_001012493.1"
                     /db_xref="GeneID:363866"
                     /db_xref="RGD:1309449"
                     /translation="MWLLLHVVLLMEVKDFTLAGSNMVAPLCPKGYFPCGNLTKCLPR
                     AFHCDGVDDCGNGADEDNCGDTSGWTTIFGTVHGNVNKVTLTEECFLSRYPQHCYCRE
                     NELECVNADLKAVPKVSSNVTLLSLKKNKIHRLPVKVFSKYAELRKIFLQHNCITHIS
                     RRAFLGLHNLQILYLSHNCITSLRPGIFKDLHQLAWLILDDNPITRISQRSFTGLNSL
                     FFLSMVSNRLEALPERLCAQMPQLNWVDLANNGIKFITNSTFLTCDSLTVLFLPRNQI
                     GFVPEKTFSSLKNLGELDLSSNVIAKLPAHLFSDLHLLQKLNLSSNPLLYVHKNQFGS
                     LKQLQSLDLERIEIPNINTGMFRPMKNLSHIYLKTFRYCSYVPHVRICMPSTDGISSS
                     EDLLANSILRVSVWVIAFITCAGNFLVIAVRSLIKAENTTHAMSIKILCCADCLMGVY
                     LFSVGVFDIKYRGQYQKYALLWMESVPCRLLGFLATLSTEVSVLLLTFLTLEKFLVIV
                     FPFSNLRLGKRQTAVVLASIWVAGFLIAAVPFTSEDYFGNFYGKNGVCFPLHYDQAED
                     FGSRGYSLGIFLGVNLLAFLVITFSYVTMFCSIQKTALQTAEVRSHIGKEVAVANRFF
                     FIVFSDAICWIPVFVVKILSLLQVEIPGTITSWIVVFFLPVNSALNPILYTLTTSLFK
                     DKLKQLLHKHRRKSIFKVKKKSLSASIVWTDDSSLKLGVLNKIALGDSIMKPVSP"
     exon            120..266
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            267..344
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            345..450
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            451..522
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            523..594
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            595..666
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            667..738
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            739..810
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            811..882
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            883..954
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1026
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1098
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1170
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1400
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1811
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..2030
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..3265
                     /gene="Rxfp2"
                     /gene_synonym="Gpcr; Lgr8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtccacaggc tcagtgctgc aaatctacag gtctttcctc tgacactgga cacctctcta
       61 gctcacgccg gctgcaatgt ggcttctact tcatgtcgtc cttctgatgg aggtcaaaga
      121 tttcacactg gctggcagca atatggtggc tcctctgtgc ccaaaagggt atttcccctg
      181 tgggaatctc accaagtgct taccccgagc ctttcactgc gacggcgtgg atgattgcgg
      241 gaatggtgcg gatgaggaca actgtggtga cactagtgga tggacgacca tatttggcac
      301 agtccatgga aatgtcaata aagtgacatt aacagaggag tgctttctca gccggtaccc
      361 acagcactgt tactgcagag aaaatgaact agaatgcgta aacgccgact taaaagctgt
      421 gccaaaggtt tccagcaacg taacattact atctcttaag aaaaacaaaa ttcacagact
      481 tccagtcaag gttttcagca aatacgcaga actcagaaag atattcctcc agcacaactg
      541 catcacacac atctccagga gagcgttcct tggattacat aacctacaga tactgtatct
      601 cagccataac tgcattacct ctctcaggcc tgggatattc aaagacttgc atcagctcgc
      661 ttggctaatt ttagatgaca acccaatcac cagaatctca cagaggtcct ttactggatt
      721 aaactccttg tttttcttgt ccatggtgag taaccggctg gaggcccttc ctgaaaggct
      781 gtgcgcccag atgcctcaac tcaactgggt ggatctggca aacaatggga taaagttcat
      841 aacgaactcc accttcctga cgtgcgactc gctcacggtt ctgtttctgc ctagaaatca
      901 aattggtttt gttccagaga agacattttc ttcattaaaa aatttaggag aactggacct
      961 gtctagcaat gtgatagcaa aactaccagc ccaccttttc agcgaccttc accttctcca
     1021 aaagctgaac ctgtcgtcca accctcttct gtatgtccac aagaaccagt ttggaagtct
     1081 caaacaactt cagtctctag acctggaaag gatagagatt ccaaacataa acaccggaat
     1141 gttccggccc atgaagaacc tttctcacat atatttgaaa acctttcggt actgctccta
     1201 tgttccccat gtccgaatct gtatgccctc gactgatggt atatcttcat ctgaggacct
     1261 cttggctaac agtatcctca gagtgtctgt ctgggttata gctttcatta cctgcgctgg
     1321 gaatttcctc gtcatagccg tgagatctct cattaaggct gagaatacga ctcacgctat
     1381 gtccatcaaa atcctttgtt gtgccgactg cctgatgggg gtgtacctgt tctccgtggg
     1441 cgtctttgac atcaagtacc gagggcagta tcagaagtat gcgctgctgt ggatggagag
     1501 tgtaccctgc cgcctgctgg gcttcctggc cacgctgtcc acagaggtct cggtgctgct
     1561 gctgacattc ctgaccctgg agaagttcct ggtcatcgta ttccctttca gcaacctgcg
     1621 cctgggcaag cgtcagacgg ccgtggtcct cgccagcatc tgggtggcgg gatttctcat
     1681 agcggccgtt cccttcacca gcgaggatta ttttggcaac ttttatggga aaaacggagt
     1741 ctgcttccca cttcattacg accaagcaga agattttgga agtagagggt actcccttgg
     1801 gattttccta ggcgtgaact tgctggcttt cctcgtcatc acgttctcct atgtcaccat
     1861 gttctgctcc attcagaaaa cagccctcca gactgcagaa gtgaggagcc acatcgggaa
     1921 ggaggtggct gtcgccaacc ggttcttttt catcgtgttc tctgacgcca tctgctggat
     1981 tcctgtgttt gtcgttaaaa tcctgtctct ccttcaagta gagataccag gcacaatcac
     2041 ttcctggatc gtggtttttt tccttccggt aaacagcgcc ttaaacccca tcctctacac
     2101 tctcaccacc tcccttttta aggacaagtt gaaacagttg ctgcacaaac atcggaggaa
     2161 atccattttc aaagttaaga agaaaagtct atctgcttcc attgtgtgga cagatgactc
     2221 ttcacttaaa cttggagtgt tgaacaaaat agcccttggg gacagtataa tgaagccggt
     2281 ctccccgtag tggttttgga tctctggact ttgagggtac tacctaaaac aaggtacaat
     2341 ttggggaaga tggcatctga gacggtttcc atcttggccg gcagacacct gttcagcacc
     2401 agggacacag tgcaacggct gttggccctg cattccgggt gacagctcta tgacctgtgg
     2461 accaagctga gaatagcatc acaagcctct ccttcctgtc agcccagacc tggcacgcac
     2521 aagtaaatcc atgtagactg ctacatggtg gatctccagg tagtcagcac tgcccgccta
     2581 accccggggt gcccacatgt agccactgcc atggccttct cttacatcac gttgcactgt
     2641 ctgttaagta tgggcatccg tgacatctta ttccaatgtg accatcacga cacaaaatga
     2701 acatctttgt caccacactt tcggtagcga atgaaatcat cagcatctta ttggtacttt
     2761 tgacagcatc tgcagggttc tggaatagca actttgaaca cagttcctca caccagttta
     2821 tcatgactag catgaccaga tgagcagaag gcatgctgtg ggagcctaga gccgttgcct
     2881 cttggcctat ttaagctcag aaaagctgag atcttagacg gttttttttt taaaggtacc
     2941 catggagcaa aggaaatcag aaacttgaac ttccccaaag agttatcatt ctcaaatgct
     3001 ttagaacaat atttctattg ctggtaagtt ctcttctcat ttagcacaga gacaaggaga
     3061 gttcaaaggt gacacaggat gatgggtctg atctggggcc attcactcaa ttgcacaaat
     3121 tcccccccag tcccacacag aaatgccaga agcgctggct ttgttttttg aaagtgaaaa
     3181 atgtttatga agacttggac acttttatac tgaaagttta taaatgtggg ggaaaagaat
     3241 actatgaata aatttatatt ttgca
//
