LOCUS       XM_006235262            4381 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus R-spondin 4 (Rspo4), mRNA.
ACCESSION   XM_006235262
VERSION     XM_006235262.4
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051338.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_006235262.5.
            
            On Jan 21, 2021 this sequence version replaced XM_006235262.3.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4381
                     /gene="Rspo4"
                     /note="R-spondin 4; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 6 ESTs, 4 Proteins, and
                     98% coverage of the annotated genomic feature by RNAseq
                     alignments, including 5 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:499918"
                     /db_xref="RGD:1563415"
     CDS             169..855
                     /gene="Rspo4"
                     /codon_start=1
                     /product="R-spondin-4"
                     /protein_id="XP_006235324.1"
                     /db_xref="GeneID:499918"
                     /db_xref="RGD:1563415"
                     /translation="MRAPLCLLLLLAHAVDMLALYRRKKQAGTGLGGNCTGCVICSEE
                     NGCSTCQQRLFLFIRREGIRQYGKCVHDCPLGFFGIRGQEANRCKKCGATCESCFSQD
                     FCIRCKRRFHLYKGKCLPTCPPGTLTHQSTRECQEECEPSPWGSWSPCIHNGKTCGSG
                     WGLETRVREAGPAKQEEMASCRVISESRKCPIKRPCPGERNPRQKNRKDRRQRKDRKL
                     ERRLHQHGSQ"
ORIGIN      
        1 cgcgctgacg ccctcactcg acccaccgcg ccctgggcgc accggcgggt ccctggtgac
       61 tgcagcgacc agcgaccagc gacccgcgga tgctcaacgc gtgccagaag ctgtcgcctc
      121 acaggggctg ccgggccgtc ggggagccag gagcaccaga ctgcccagat gcgggcgcca
      181 ctctgcctgc tgctgctcct cgcccacgca gtggacatgc tcgccctgta ccgaaggaag
      241 aagcaagcag gcactggtct ggggggcaac tgcacaggct gtgtcatatg ctcggaggag
      301 aatggctgct ccacctgcca gcaaaggctt ttcctcttca tccgccggga aggtatccgt
      361 cagtacggca agtgtgtgca tgactgtccc cttggcttct tcggaatccg tggccaggag
      421 gccaacaggt gcaaaaaatg cggggccaca tgcgagagct gtttcagcca ggacttctgt
      481 atccgctgca aaaggcggtt tcacctctac aaagggaagt gtctgcccac ctgtccgcca
      541 ggcaccttga cccaccagag tacccgggag tgccaggagg agtgcgagcc gagcccttgg
      601 ggcagctgga gcccctgcat acacaatggg aagacctgtg gctcaggctg gggcctagag
      661 acccgggtgc gggaggctgg cccagccaag caggaagaga tggcaagttg ccgagtgata
      721 tctgagtcca ggaaatgtcc tataaagagg ccctgcccag gagaaagaaa tcccaggcag
      781 aagaaccgga aggatcggcg ccagcgcaag gacaggaagt tggagcgcag gctgcaccag
      841 catggctccc agtgaccatc tcaagctctg gcctctgcct ttggcttgct gcacttccac
      901 tgtgggctcc ttccttctcc cctgccccgc tctttctcca catccttccc tctttctgtc
      961 ggctactttt cccatctgcc cggtctttgc gttatatttt atcgatctcc cttttcctct
     1021 ccccgtcact tccttcttca catccttatt tcttctttgg aacttccctt ctctccgtat
     1081 acgtttttct tcatttcctt ctgttttcct ttctgctctt cactgtccat atcagacata
     1141 agagactgag cttgtgtccc ccgtgtggct ctctgcacct caaaacaaac tttttcttct
     1201 agatcttttt gtgactctgt cctgactccg tctttgcaaa gctacaattt aggaggtata
     1261 ggtttgggtg caacttgaca agtcctgaga gctctgcttc tagccagaga gaccctgcag
     1321 ctggagttaa gaactgctca ccaggccagt ccctcaaggc cgattctaca gcattcagcc
     1381 attgcctctt cgatgaggcc accagagaca ggttctggaa cctccgaggg cccggcctga
     1441 accccttatc caggatggtg gcataggagc cactaggggt cctttggcta gacagtggcc
     1501 tcgggcccac caccacaccc ccttggaagt gcctgcaaca cccgctctga tcaccctccc
     1561 accgctccac ccacacctcc taccccaccc ccaccccact ctgctctccc tatcgtagtg
     1621 ccacgacctt gcaacaactg accagcctcc caaggccttt ggagacccaa gcacaggatc
     1681 agatggcact gtgattcctg ttattcaata atcccgaagg ctactgaagc taaatggaac
     1741 ttaagaggtg gtcgcgccca ccccattcac caccctgcta ccttacggcc catcgtcccc
     1801 atagagactg acgtctaagg aagtgatggg tactctgtct tcggtccgca catcacgtct
     1861 atgaagaagt tcaagtgtgt ttggggagta gagaggattg ctgggttagc agttgggatg
     1921 gcctcccact tacccaccct ccacgtggat accctgagtg ttttctttta ctgtgatgcc
     1981 cccaactcca ggcaggaagg atactgggag gagccttcag gtaaaacaaa ccaaatggca
     2041 agtctgtcct caccctgcca ttggccaatg agctgccatt gttgctggga ggagtctggg
     2101 cattaggacc agagacagct ggggaggatg tggtataggc aagggagaag ctccatgtga
     2161 tataatgtca ctctctcaca cagtgacctg cacagccacc ctacacttgg caggtggggg
     2221 tccccaccac cactgctgac cctgtagctg tgctcctcgt tggcgcccca cgagaacatc
     2281 tggataccca gcttgtcagg ccaggggccc aggtttctgc agaagttcgc acctctgaaa
     2341 tgcttccctg ttgttttgag actcccagct actttttaaa gaaagataaa atgaaagaaa
     2401 cttgtgaaga cttcttgggc atcagatagt gttaggaacc accatgccca gaggttcaca
     2461 atggtggact tcccggggca gcttctgcag agtgttctgg ctgagagtag ccaaggttca
     2521 tggggactga ctgagaacag acagggttcc tgctggagtg ctgaccagtt cctcctaaga
     2581 aggaggcttt caggagctcc atcattaaag tgcttgcttt gcaagcaaag gacctgagtt
     2641 tgacccccag cacccacatg aaaaagccag atatagtggc atgcccttgt gtctcagcac
     2701 tggggaggta gagacacaga tgcctgggcc ttgctggcca gtcggcctgc cccaaggggc
     2761 aagatccagg ccagtaaaga gccttgtttc aaaaaatgtt agccagcact gagcgacacc
     2821 tgagttatcc tctggcctct atacacttgt acagaacaca tacacatgca tgtgcacaca
     2881 cacgcacata tacagagaga gggaaggagg gagggaggga gggagggaga gagagagaga
     2941 gagagagaga gagagagaga gagagagaga acacttgagc tttagccaga tgcaaaagtc
     3001 agaaactctt agactcctga attagataga gcccaggcct caacttgtga agcacggtct
     3061 cattgactca tgggaataac ctgggcttct ggtgacctga tgtaaaccca gcctgtgagc
     3121 agttagctgg gagtctgcgc ctgcgcctgt tctggacttc tgttttacct tgtgatggat
     3181 gaggaagaga caaggctggt ctggctccat tgtatgcatc tggaaggttc tgcactgtgc
     3241 tgggctttaa gggagtttca cagcttcaga tttcagggtc ccacccagac ttatacatta
     3301 tgaacaagct gagaaatgac taagaagaac ttattctaga ataagttggg gggaagggca
     3361 cctaggtttc caggatttgg attaaggacc acagcacgag ctctgaccgc agagctcaaa
     3421 ggtctgagtc tggatagatg ggaaatccag gtatgttctg tctggagttt cccgctaagt
     3481 tcaaacccta tgatactgga ggctctaaat tccatagttg ttacagggca agagtggaag
     3541 gtcttgggcc tgcaattatc tagctatgct ctcccttgtg aggtgaggga cctgatacct
     3601 gatactcttt tcctaaccca gggctctgga tattttttaa atatttttta atgttttaaa
     3661 gtttaaaaaa ttctcccaga gccctcactg taacctaagg cttcagataa gccttcatgc
     3721 tacatctctc ccaccctcct ggtaagaaaa gatccaggcc cagacaatgc aaggaacatt
     3781 cagagatgtc ctacagctgg ctggagtcat ggagtcgttg tttggtccct tgataatgtt
     3841 ctagtgactg acaatgtcgc tttgcccttc ctactccgtt cctgcaaggc tgtaccagtc
     3901 ttcagagtca cccctcccat ctcaacccaa tccttcacct tgggctgcct ggtgttccca
     3961 tatacagcca ctagagggtg tcgctcaatc agtaactgcc agcctggtcg ttgcccactc
     4021 tcttcaattt ggggacatat tgtagtcctg gggtgggggg atagggagag cagtgacttc
     4081 agtgaaatgg ccttcactgc agcacacaat gtctaggtcc caagccattc caagcccaga
     4141 acagaaacct gactcagaag acaagagttc aacattccag gagagccatg aatgacctgc
     4201 agacaccagc ccccaggcaa cagtgaacac tccagataat tttaagacgg tttcattcat
     4261 cccgtgggac ccttctgact tgggaatgtg tatattctaa aaataaaaac gatagtgatg
     4321 gtggagatgg tgtccgctat cagctcagca cctactgtat ggtagagtgc taagagccct
     4381 a
//
