LOCUS       NM_053338               1471 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Rattus norvegicus RRAD, Ras related glycolysis inhibitor and
            calcium channel regulator (Rrad), mRNA.
ACCESSION   NM_053338
VERSION     NM_053338.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Oda,T., Niikura,T., Fukui,T., Arakura,M., Oe,K., Mifune,Y.,
            Hayashi,S., Matsumoto,T., Matsushita,T. and Kuroda,R.
  TITLE     Ras associated with diabetes may play a role in fracture nonunion
            development in rats
  JOURNAL   BMC Musculoskelet Disord 20 (1), 602 (2019)
   PUBMED   31830958
  REMARK    GeneRIF: expression increased significantly in the nonunions,
            compared to that in the standard healing fractures, at
            post-fracture days 14, 21, and 28
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Zhang,J., Chang,L., Chen,C., Zhang,M., Luo,Y., Hamblin,M.,
            Villacorta,L., Xiong,J.W., Chen,Y.E., Zhang,J. and Zhu,X.
  TITLE     Rad GTPase inhibits cardiac fibrosis through connective tissue
            growth factor
  JOURNAL   Cardiovasc Res 91 (1), 90-98 (2011)
   PUBMED   21382976
  REMARK    GeneRIF: Rad GTPase inhibits cardiac fibrosis through connective
            tissue growth factor in failing hearts.
REFERENCE   3  (bases 1 to 1471)
  AUTHORS   Sun,Z., Zhang,J., Zhang,J., Chen,C., Du,Q., Chang,L., Cao,C.,
            Zheng,M., Garcia-Barrio,M.T., Chen,Y.E., Xiao,R.P., Mao,J. and
            Zhu,X.
  TITLE     Rad GTPase induces cardiomyocyte apoptosis through the activation
            of p38 mitogen-activated protein kinase
  JOURNAL   Biochem Biophys Res Commun 409 (1), 52-57 (2011)
   PUBMED   21549102
  REMARK    GeneRIF: Rad-triggered apoptosis was mediated by the activation of
            p38 MAPK.
REFERENCE   4  (bases 1 to 1471)
  AUTHORS   Luo,Y., Zhang,M., Zhang,J., Zhang,J., Chen,C., Chen,Y.E.,
            Xiong,J.W. and Zhu,X.
  TITLE     Platelet-derived growth factor induces Rad expression through Egr-1
            in vascular smooth muscle cells
  JOURNAL   PLoS One 6 (4), e19408 (2011)
   PUBMED   21559360
  REMARK    GeneRIF: Rad is regulated by PDGF through the transcriptional
            factor Egr-1 in RASMCs
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1471)
  AUTHORS   Wang,G., Zhu,X., Xie,W., Han,P., Li,K., Sun,Z., Wang,Y., Chen,C.,
            Song,R., Cao,C., Zhang,J., Wu,C., Liu,J. and Cheng,H.
  TITLE     Rad as a novel regulator of excitation-contraction coupling and
            beta-adrenergic signaling in heart
  JOURNAL   Circ Res 106 (2), 317-327 (2010)
   PUBMED   19926875
  REMARK    GeneRIF: Results establish Rad as a novel endogenous regulator of
            cardiac EC coupling and betaAR signaling and support a parsimonious
            model in which Rad buffers Ca(v)beta to modulate LCC activity, EC
            coupling, and betaAR responsiveness.
REFERENCE   6  (bases 1 to 1471)
  AUTHORS   Yada,H., Murata,M., Shimoda,K., Yuasa,S., Kawaguchi,H., Ieda,M.,
            Adachi,T., Murata,M., Ogawa,S. and Fukuda,K.
  TITLE     Dominant negative suppression of Rad leads to QT prolongation and
            causes ventricular arrhythmias via modulation of L-type Ca2+
            channels in the heart
  JOURNAL   Circ Res 101 (1), 69-77 (2007)
   PUBMED   17525370
REFERENCE   7  (bases 1 to 1471)
  AUTHORS   Ilany,J., Bilan,P.J., Kapur,S., Caldwell,J.S., Patti,M.E.,
            Marette,A. and Kahn,C.R.
  TITLE     Overexpression of Rad in muscle worsens diet-induced insulin
            resistance and glucose intolerance and lowers plasma triglyceride
            level
  JOURNAL   Proc Natl Acad Sci U S A 103 (12), 4481-4486 (2006)
   PUBMED   16537411
REFERENCE   8  (bases 1 to 1471)
  AUTHORS   Wang,G.Y., Niu,T.H., Chen,C.Z., Li,Q.F. and Xu,X.P.
  TITLE     A novel Rad gene polymorphism combined with obesity increases risk
            for type 2 diabetes mellitus
  JOURNAL   Chin Med J (Engl) 117 (5), 770-771 (2004)
   PUBMED   15161552
REFERENCE   9  (bases 1 to 1471)
  AUTHORS   Yuan,X., Yamada,K., Ishiyama-Shigemoto,S., Koyama,W. and Nonaka,K.
  TITLE     Analysis of trinucleotide-repeat combination polymorphism at the
            rad gene in patients with type 2 diabetes mellitus
  JOURNAL   Metabolism 48 (2), 173-175 (1999)
   PUBMED   10024077
REFERENCE   10 (bases 1 to 1471)
  AUTHORS   Caldwell,J.S., Moyers,J.S., Doria,A., Reynet,C. and Kahn,R.C.
  TITLE     Molecular cloning of the human rad gene: gene structure and
            complete nucleotide sequence
  JOURNAL   Biochim Biophys Acta 1316 (3), 145-148 (1996)
   PUBMED   8781531
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FM054321.1, CK602305.1, FM055705.1 and CK840773.1.
            
            On Aug 4, 2012 this sequence version replaced NM_053338.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12187.1, EV767675.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                FM054321.1         2-29
            29-679              CK602305.1         1-651
            680-1212            FM055705.1         21-553
            1213-1471           CK840773.1         1-259               c
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19p14"
     gene            1..1471
                     /gene="Rrad"
                     /note="RRAD, Ras related glycolysis inhibitor and calcium
                     channel regulator"
                     /db_xref="GeneID:83521"
                     /db_xref="RGD:69357"
     exon            1..157
                     /gene="Rrad"
                     /inference="alignment:Splign:2.1.0"
     exon            158..539
                     /gene="Rrad"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1096
                     /gene="Rrad"
                     /EC_number="3.6.5.2"
                     /note="RAD1; ras associated with diabetes; Ras-related
                     associated with diabetes"
                     /codon_start=1
                     /product="GTP-binding protein RAD"
                     /protein_id="NP_445790.2"
                     /db_xref="GeneID:83521"
                     /db_xref="RGD:69357"
                     /translation="MTLNGGSGAGGSRSAGRERDRRRGSTPWGPAPPLHRRSMPVDER
                     DLQAALTPGSLAATAAGTRTQGQRLDWPEGSSDSLSSGDSGSEDGVYKVLLLGAPGVG
                     KSALARIFGGIEDGPEAEAAGHTYDRSITVDGEEASLMVYDIWEQDGGCWLPGHCMAM
                     GDAYVIVYSITDKGSFEKASELRVQLRRARQTDNVPIILVGNKSDLVRSREVSVDEGR
                     ACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEDNARRQAGTRRRESLGKK
                     AKRFLGRIVARNSRKMAFRAKSKSCHDLSVL"
     exon            540..613
                     /gene="Rrad"
                     /inference="alignment:Splign:2.1.0"
     exon            614..818
                     /gene="Rrad"
                     /inference="alignment:Splign:2.1.0"
     exon            819..1458
                     /gene="Rrad"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgaggggctg agaaggccat caaggagaaa aaggttgcag acgtgacaga gacaaggctc
       61 cgagtctgaa caggggtctg cgagtgggtc tatctgattc cccagtggtc tgtgccgcag
      121 cgctgatcaa gtgctgatag catctgtgca agagtcgacg gcgtccgaca cgatgaccct
      181 gaatggaggc agcggagcgg gcggcagccg tagcgcgggc cgggagcgcg accgtcgtcg
      241 aggcagcacc ccatggggcc cggcaccccc actgcaccgc aggagcatgc cggtggacga
      301 gcgagacctt caggcggcgc taaccccggg ctccctggca gcgactgcag ctggaactcg
      361 gacacagggg cagaggctgg actggcctga aggctcctcc gattcgctca gttctggaga
      421 cagcggctca gaggatggcg tttacaaggt gctgctgctg ggggcacctg gtgtgggcaa
      481 gagcgctctg gcgcgcatct ttggtggtat agaagacgga cctgaagcag aagcagcagg
      541 gcacacatat gaccgttcta tcactgtgga tggagaagaa gcatcactaa tggtctatga
      601 catttgggaa caggatggag gctgctggct gcctggccac tgcatggcca tgggggatgc
      661 atatgtcatt gtgtactcaa taacggacaa gggcagcttt gagaaagcct cagaactccg
      721 ggtccagctg cggcgggcac ggcagacaga caatgtgccc atcatcctag tgggcaacaa
      781 gagcgatctg gtgcgctctc gtgaggtctc tgtggatgag ggccgggcct gcgcagtggt
      841 cttcgactgc aagttcatcg agacctccgc agcactgcat cacaacgtcc aggcactgtt
      901 cgagggtgtt gtgcgtcaga tacgcctgcg cagggacagc aaagaggata atgctcgtcg
      961 acaagctggc actcgacgac gggagagcct tggcaagaag gccaaacgct ttctgggccg
     1021 catagtagct cgcaacagcc gcaagatggc ctttcgtgcc aagtccaagt cctgccatga
     1081 cctctctgtt ctctaaggcc aacggccccc ttgtttgtgg gtcagtagat ggatggaagg
     1141 gctggcccag ccaactgctc taaatgtctc agagttggcc caccctccgt atttctcaga
     1201 actgtcaact atgcacagcc tgtctcacgg tcatggacag atagtgctga ccagggaggc
     1261 aactccaaac ggccatcgag gcctgggcct atggagatgc atgtgcaggc ccacgtgcat
     1321 accgagcagc ctgaaccctg ctcttggaca ggactctgcc tttctccatg tgggtgtctg
     1381 ccctggaggg aggctgttct atatgcttgc tgtttgttta catgcagatt tttgtaataa
     1441 agactatttc ctgacaaaaa aaaaaaaaaa a
//
