LOCUS       NM_001025284            1830 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus RNA pseudouridine synthase D4 (Rpusd4), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001025284 XM_235991
VERSION     NM_001025284.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Arroyo,J.D., Jourdain,A.A., Calvo,S.E., Ballarano,C.A.,
            Doench,J.G., Root,D.E. and Mootha,V.K.
  TITLE     A Genome-wide CRISPR Death Screen Identifies Genes Essential for
            Oxidative Phosphorylation
  JOURNAL   Cell Metab 24 (6), 875-885 (2016)
   PUBMED   27667664
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 1830)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098023.1.
            
            On Jul 13, 2005 this sequence version replaced XM_235991.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098023.1, SRR8487231.7755.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q21"
     gene            1..1830
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /note="RNA pseudouridine synthase D4"
                     /db_xref="GeneID:315550"
                     /db_xref="RGD:1311268"
     exon            1..196
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1141
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /EC_number="5.4.99.12"
                     /note="RNA pseudouridylate synthase domain-containing
                     protein 4; mitochondrial RNA pseudouridine synthase
                     Rpusd4; RNA pseudouridylate synthase domain containing 4"
                     /codon_start=1
                     /product="pseudouridylate synthase RPUSD4, mitochondrial"
                     /protein_id="NP_001020455.1"
                     /db_xref="GeneID:315550"
                     /db_xref="RGD:1311268"
                     /translation="MAAPLLGSPGLQVLSMSSRTGKLFTPSSRSFCSRATSSRPLNAQ
                     RLAEKLRAQKQEQKTKEMRVPTNPVQRRVQELVRFTQQLQRVHPNVLAKELSRRILHQ
                     DKDLVVINKPYGLPVHGGPGVQLCISDVLPILAKMLHGHKAEPLHLCHRLDKETTGVM
                     VLAWEKDMAHQVQELFRTRQVEKKYWAITVRVPLPSAGVVDIPIMEKEVAGQQQHHKM
                     TLRPSYRMDNGKMVKVRASRDAHVAVTQYQVLSSTPSSALVELQPVTGIKHQLRVHLA
                     FGLDCPILGDHKYSDWNRLAPQKLSAGTLKKLGLQQSKARYIPLHLHARQLILPALGS
                     RTEELLLACKLPHFFARSLRRLGLDMPNEDQSRSHEARHVEAR"
     exon            197..362
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     exon            363..564
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     exon            565..658
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     exon            659..803
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     exon            804..901
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1801
                     /gene="Rpusd4"
                     /gene_synonym="RGD1311268"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcaagatg gccgcgccct tgttgggttc ccctggtctc caggtcctga gtatgagttc
       61 gcgtaccggg aaattgttca ctcccagctc caggtcgttt tgttccagag ctacttcctc
      121 caggcccctg aatgctcaga gactggcgga gaagctccga gctcagaaac aagagcaaaa
      181 gacgaaggaa atgcgggtgc ccaccaaccc cgttcagcgg cgggtgcagg agctagtgag
      241 attcacacag cagctacagc gtgttcaccc caatgtgctt gctaaggaac tgagccgaag
      301 gattctccac caggacaaag accttgtggt cattaataaa ccctatggtc tccctgtcca
      361 tggtggccct ggagtccagc tctgcatcag tgatgttctg cccatcttgg caaagatgct
      421 tcatggccac aaggcagaac ctttgcatct gtgccaccgt ctggacaaag agaccacagg
      481 tgtaatggtt ttggcttggg agaaggacat ggcacatcaa gtccaggagc tgtttagaac
      541 ccgccaggtg gaaaagaagt actgggccat cactgtgcgt gttcccttgc cctcggcagg
      601 agttgtggat atccccatca tggagaagga agtggctggg cagcagcaac accacaagat
      661 gacgctacgt ccaagttacc gcatggacaa tgggaaaatg gtaaaggtac gggccagccg
      721 ggatgcacac gtcgcggtaa ctcagtacca ggtgctgagc agcaccccgt cctccgccct
      781 tgtggagctg cagcctgtca ctggaataaa acatcagctc cgagttcacc tggcctttgg
      841 gttggattgc ccaatcctcg gtgatcacaa atactcagac tggaataggt tggcccctca
      901 gaaactttct gctggcacac tgaagaagct ggggctacaa cagtcaaagg cccgctacat
      961 ccctcttcat ctgcatgcca ggcagctcat cctgcctgct ctgggctcca ggacagagga
     1021 actcctcctg gcctgcaagc ttcctcactt ctttgcacgt tccctgcgtc gtctgggttt
     1081 agatatgcca aatgaggatc aaagcagaag ccatgaggcc aggcatgtgg aagcacggtg
     1141 aagactgttg ggcagtttgg ccccagagct ctgctgtatt tatttcatgg attctgctaa
     1201 cttctcactt agaacagatg acaagagccg ggtggggtaa gttggaaaaa cttggccact
     1261 tcaggactgt aatggagaac gaagtcttat ttatggcagt cgggctgtaa tgtgggaaag
     1321 cctgctggag tctcctccat aggtcacttt gtcaagatgg aggagagcct aggaggcagc
     1381 agactgaacc agtaaaggag aaccaaagga tggatctgct taggacttgt cttcatccaa
     1441 caggaaggaa agctgagcta tcaggagtgc tccgttggaa agatgtcttg gataaacatg
     1501 catcgagggc ccaggggttt tgtccactag agtgtgggtg ctgtactgcc ccagcagtga
     1561 gcctgtgaaa gggttgttgg ggtgaggtaa gtgacaggca gctaacataa gtgagtgaag
     1621 ctggcagggt ggaaggcagc ggacaacaga ccaaactcaa gctgtctaag ggttattagc
     1681 accagttgat tgttgccctg tgggcaagtt tactattttc ttttagagaa ccaggaaatc
     1741 cagatatttc tgcgaaatct cccaatctat tgctatctaa ataaaaacat tttgaaatat
     1801 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
