LOCUS       NM_001398862            1262 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus RNA pseudouridine synthase D3 (Rpusd3),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001398862 XM_006237044
VERSION     NM_001398862.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Arroyo,J.D., Jourdain,A.A., Calvo,S.E., Ballarano,C.A.,
            Doench,J.G., Root,D.E. and Mootha,V.K.
  TITLE     A Genome-wide CRISPR Death Screen Identifies Genes Essential for
            Oxidative Phosphorylation
  JOURNAL   Cell Metab 24 (6), 875-885 (2016)
   PUBMED   27667664
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 1262)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Dec 27, 2021 this sequence version replaced XM_006237044.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.1620878.1,
                                           SRR26360181.3125745.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               JAXUCZ010000004.1  148118095-148118206 c
            113-258             JAXUCZ010000004.1  148117677-148117822 c
            259-303             JAXUCZ010000004.1  148116832-148116876 c
            304-403             JAXUCZ010000004.1  148116652-148116751 c
            404-511             JAXUCZ010000004.1  148116090-148116197 c
            512-596             JAXUCZ010000004.1  148115933-148116017 c
            597-720             JAXUCZ010000004.1  148115652-148115775 c
            721-860             JAXUCZ010000004.1  148115160-148115299 c
            861-1262            JAXUCZ010000004.1  148114202-148114603 c
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..1262
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /note="RNA pseudouridine synthase D3"
                     /db_xref="GeneID:362416"
                     /db_xref="RGD:1307582"
     exon            1..112
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..1052
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /note="isoform 1 is encoded by transcript variant 1; RNA
                     pseudouridylate synthase domain-containing protein 3; RNA
                     pseudouridylate synthase domain containing 3;
                     mitochondrial mRNA pseudouridine synthase RPUSD3"
                     /codon_start=1
                     /product="mitochondrial mRNA pseudouridine synthase RPUSD3
                     isoform 1"
                     /protein_id="NP_001385791.1"
                     /db_xref="GeneID:362416"
                     /db_xref="RGD:1307582"
                     /translation="MEALRALRSVSVVWRPGLGSSARRRDAGFGTEARRRPQPQPHRS
                     SKRKDLVEDQPFPGLLRAENLGLEELAQVLRAAVVDQKGPLVTLNKPQGLPVTGRPGE
                     LTLLSVLPWLSQALGLEHQELQVVRAPGKEASGLVLLSSCPQTTSHLQKFFTHSRRAQ
                     RPTATYCAITDGVPEPSEGTVHIALRLERMDGIDLAVPVTSPSRKDIQEGVKRTLSHF
                     HVTATGCGCALVQLQPLTVFPSQLQVHMALQLCPILGDHTYAARVGSVLGQRFLWPAE
                     TTKPQRQVLDEALLRYLHLSPSQVAQMPLHLHLHRLLLPGTRPRDPPSELLAPLPPYF
                     SRTLQCLRLSQQ"
     exon            113..258
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            259..303
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            304..403
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            404..511
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            512..596
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            597..720
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            721..860
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1262
                     /gene="Rpusd3"
                     /gene_synonym="RGD1307582"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttgattcggc tatggaagcc ctgcgagctt tacgtagtgt ctctgtggtc tggcggccgg
       61 ggctggggtc tagcgcacgg cggagggatg caggcttcgg caccgaggcc cggcgtcggc
      121 ctcaacctca acctcaccgc tccagcaagc gcaaggacct agtggaggac cagcctttcc
      181 ccgggctgct gcgggcagag aacctcggtc tggaagagct ggctcaagtc ctgagagcag
      241 ctgtggtgga tcagaaagga cctctggtga cactgaacaa gccacagggt ctgcctgtga
      301 cagggagacc aggagagctg acattgctgt ctgtgctgcc atggctgagc caggccctgg
      361 gactcgaaca ccaggagctc caggttgtgc gagcacctgg aaaggaggcc tctgggcttg
      421 tgctcctctc cagctgtccc cagacaacaa gccacctgca gaagttcttt acccactcaa
      481 ggagagccca gagacccaca gccacctact gtgccatcac tgatggtgtc cctgagcctt
      541 ctgaggggac agtccacata gctctgaggc tggaacgaat ggatggcatt gatcttgcag
      601 tcccagtgac gtccccatct cgaaaggaca tccaggaagg tgtcaaaagg accctcagcc
      661 atttccatgt gacggccaca ggctgtggct gtgctctggt ccagctgcag ccactgacag
      721 tcttccccag tcagctgcag gtgcacatgg ctctacagct ctgcccaatt cttggagacc
      781 acacctatgc ggctcgcgtg ggcagcgttc tgggccagcg ctttctgtgg ccagcagaga
      841 ctaccaagcc ccagagacag gtcctggatg aagccctgct tagatacctc cacctgagtc
      901 cttcccaagt ggcccagatg cctttgcacc tgcacctgca ccgcctcctc ctcccaggca
      961 ccagacccag ggaccccccg agtgaacttc tggctcccct gcccccttac ttctctcgga
     1021 ctttgcagtg ccttcggctc tctcagcaat aggcccccgg gtgagagtag aataagagct
     1081 gtgcccccct caaggacagt gaggtggtgc tgagcacagc caagatcctg ggctccgtgg
     1141 tctgacacac ctaagatcaa atcccagaga ggccacttga ctgctgtgtg gcattgggca
     1201 ggtgacttct ctctggactt aagataataa aagttctacc tcaccgtttg gtttaccaaa
     1261 aa
//
