LOCUS       NM_001305443            1035 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus ribosomal protein S27a (Rps27a), transcript
            variant 2, mRNA.
ACCESSION   NM_001305443
VERSION     NM_001305443.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Anger,A.M., Armache,J.P., Berninghausen,O., Habeck,M., Subklewe,M.,
            Wilson,D.N. and Beckmann,R.
  TITLE     Structures of the human and Drosophila 80S ribosome
  JOURNAL   Nature 497 (7447), 80-85 (2013)
   PUBMED   23636399
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 1035)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 1035)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   5  (bases 1 to 1035)
  AUTHORS   O'Donohue,M.F., Choesmel,V., Faubladier,M., Fichant,G. and
            Gleizes,P.E.
  TITLE     Functional dichotomy of ribosomal proteins during the synthesis of
            mammalian 40S ribosomal subunits
  JOURNAL   J Cell Biol 190 (5), 853-866 (2010)
   PUBMED   20819938
REFERENCE   6  (bases 1 to 1035)
  AUTHORS   Louie,D.F., Resing,K.A., Lewis,T.S. and Ahn,N.G.
  TITLE     Mass spectrometric analysis of 40 S ribosomal proteins from Rat-1
            fibroblasts
  JOURNAL   J Biol Chem 271 (45), 28189-28198 (1996)
   PUBMED   8910435
REFERENCE   7  (bases 1 to 1035)
  AUTHORS   Vladimirov,S.N., Ivanov,A.V., Karpova,G.G., Musolyamov,A.K.,
            Egorov,T.A., Thiede,B., Wittmann-Liebold,B. and Otto,A.
  TITLE     Characterization of the human small-ribosomal-subunit proteins by
            N-terminal and internal sequencing, and mass spectrometry
  JOURNAL   Eur J Biochem 239 (1), 144-149 (1996)
   PUBMED   8706699
REFERENCE   8  (bases 1 to 1035)
  AUTHORS   Chan,Y.L., Suzuki,K. and Wool,I.G.
  TITLE     The carboxyl extensions of two rat ubiquitin fusion proteins are
            ribosomal proteins S27a and L40
  JOURNAL   Biochem Biophys Res Commun 215 (2), 682-690 (1995)
   PUBMED   7488009
REFERENCE   9  (bases 1 to 1035)
  AUTHORS   Hubbard,M.J. and Carne,A.
  TITLE     Differential feeding-related regulation of ubiquitin and
            calbindin9kDa in rat duodenum
  JOURNAL   Biochim Biophys Acta 1200 (2), 191-196 (1994)
   PUBMED   8031840
REFERENCE   10 (bases 1 to 1035)
  AUTHORS   Collatz,E., Ulbrich,N., Tsurugi,K., Lightfoot,H.N., MacKinlay,W.,
            Lin,A. and Wool,I.G.
  TITLE     Isolation of eukaryotic ribosomal proteins. Purification and
            characterization of the 40 S ribosomal subunit proteins Sa, Sc,
            S3a, S3b, S5', S9, S10, S11, S12, S14, S15, S15', S16, S17, S18,
            S19, S20, S21, S26, S27', and S29
  JOURNAL   J Biol Chem 252 (24), 9071-9080 (1977)
   PUBMED   925037
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1 and FM074661.1.
            
            Summary: The protein encoded by this gene is a fusion protein that
            contains ubiquitin at its N-terminus and ribosomal protein S27a at
            its C-terminus. When the human ortholog of this protein is
            expressed in yeast, it is processed post-translationally into two
            products, a free ubiquitin monomer and ribosomal protein S27a, a
            component of the 40S ribosomal subunit. There are multiple
            pseudogenes of this gene. There is a locus on chromosome 5
            (GeneID:81777) that contains an intact copy of the open reading
            frame of this gene, that is likely to be the result of
            retrotransposition of an mRNA into the genome. The transcriptional
            status of this locus cannot be verified at the present time.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Both variants 1 and 2 encode the same
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BF281119.1, FM072030.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840115, SAMN12840129
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                JAXUCZ010000014.1  107488787-107488885 c
            100-620             FM074661.1         21-541
            621-1035            JAXUCZ010000014.1  107486664-107487078 c
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q22"
     gene            1..1035
                     /gene="Rps27a"
                     /note="ribosomal protein S27a"
                     /db_xref="GeneID:100912032"
                     /db_xref="RGD:6489478"
     exon            1..164
                     /gene="Rps27a"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..587
                     /gene="Rps27a"
                     /note="ubiquitin carboxyl extension protein 80;
                     ubiquitin-40S ribosomal protein S27a"
                     /codon_start=1
                     /product="ubiquitin-ribosomal protein eS31 fusion protein"
                     /protein_id="NP_001292372.1"
                     /db_xref="GeneID:100912032"
                     /db_xref="RGD:6489478"
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
                     FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAV
                     LKYYKVDENGKISRLRRECPSDECGAGVFMGSHFDRHYCGKCCLTYCFNKPEDK"
     exon            165..219
                     /gene="Rps27a"
                     /inference="alignment:Splign:2.1.0"
     exon            220..305
                     /gene="Rps27a"
                     /inference="alignment:Splign:2.1.0"
     exon            306..437
                     /gene="Rps27a"
                     /inference="alignment:Splign:2.1.0"
     exon            438..1035
                     /gene="Rps27a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttggtgagt gtgtgctctg tggcccggct ctggctagtg gctctaccgg tcgctctcac
       61 tggtgtggtc gggtctaatc cgtctctttt cgaatgcagg tggagcagcc gccacgatgc
      121 agatttttgt gaagaccctt acagggaaga ccatcacgct cgaggttgaa ccctcggaca
      181 ctatagaaaa tgtaaaggcc aagatccagg ataaggaagg aattcctcct gatcagcaga
      241 ggttgatctt tgctggtaag cagttggaag atggccgtac tttgtctgac tacaacattc
      301 aaaaggagtc cactctccat ctcgtgctga gacttcgtgg tggcgctaag aaaaggaaga
      361 agaagtctta caccacccca aagaagaaca agcataagag aaagaaggtc aagttggctg
      421 tgctgaaata ctataaggtg gatgaaaatg gcaaaatcag ccgacttcgt cgggaatgtc
      481 cttctgatga atgtggtgct ggagttttca tgggtagcca ctttgacagg cattactgtg
      541 gcaagtgttg tctgacttac tgcttcaaca aaccagaaga caagtagttg tgtatgagtt
      601 aataaagaga aggaactgat gtttagtatt gtgttttatt attgtttaag caatgtttct
      661 tcagctgtgt aacagtcctt ggtcaggaga gtttgatcgt aatggtaagc catcttggct
      721 ttggctttcc tggcaggcac aaaggtcaga attctttaag gggttgtggg atatcctgta
      781 gacttggaag tagtgtgatc ttgtcttacg agacatgaga gtagtctcac agtacattca
      841 agatgcttcc tgaggttggc tatgtgtgtg agctgttaaa tattttcagt gttgatggtg
      901 atttttcctc tataagaggt aagctaggca tgtggtgcat agttaaggtt tcagcacttg
      961 ggagacttca gcaggagatt tgctgccagc ttgtgccagc aagacactgt atttaaataa
     1021 agaaacgaaa acaaa
//
