LOCUS       NM_053562               2147 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus retinoid isomerohydrolase RPE65 (Rpe65), mRNA.
ACCESSION   NM_053562
VERSION     NM_053562.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Choi,E.H., Suh,S., Sander,C.L., Hernandez,C.J.O., Bulman,E.R.,
            Khadka,N., Dong,Z., Shi,W., Palczewski,K. and Kiser,P.D.
  TITLE     Insights into the pathogenesis of dominant retinitis pigmentosa
            associated with a D477G mutation in RPE65
  JOURNAL   Hum Mol Genet 27 (13), 2225-2243 (2018)
   PUBMED   29659842
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Kim,H.L., Nam,S.M., Chang,B.J., Nahm,S.S. and Lee,J.H.
  TITLE     Ultrastructural Changes and Expression of PCNA and RPE65 in Sodium
            Iodate-Induced Acute Retinal Pigment Epithelium Degeneration Model
  JOURNAL   Neurochem Res 43 (5), 1010-1019 (2018)
   PUBMED   29569173
  REMARK    GeneRIF: The damaged retinal pigment epithelium showed earlier
            expression of PCNA and RPE65 than photoreceptor out segments
REFERENCE   3  (bases 1 to 2147)
  AUTHORS   Shyam,R., Gorusupudi,A., Nelson,K., Horvath,M.P. and Bernstein,P.S.
  TITLE     RPE65 has an additional function as the lutein to meso-zeaxanthin
            isomerase in the vertebrate eye
  JOURNAL   Proc Natl Acad Sci U S A 114 (41), 10882-10887 (2017)
   PUBMED   28874556
REFERENCE   4  (bases 1 to 2147)
  AUTHORS   Sheridan,C., Boyer,N.P., Crouch,R.K. and Koutalos,Y.
  TITLE     RPE65 and the Accumulation of Retinyl Esters in Mouse Retinal
            Pigment Epithelium
  JOURNAL   Photochem Photobiol 93 (3), 844-848 (2017)
   PUBMED   28500718
REFERENCE   5  (bases 1 to 2147)
  AUTHORS   Takahashi,Y., Moiseyev,G. and Ma,J.X.
  TITLE     Identification of key residues determining isomerohydrolase
            activity of human RPE65
  JOURNAL   J Biol Chem 289 (39), 26743-26751 (2014)
   PUBMED   25112876
REFERENCE   6  (bases 1 to 2147)
  AUTHORS   Beatrice,J., Wenzel,A., Reme,C.E. and Grimm,C.
  TITLE     Increased light damage susceptibility at night does not correlate
            with RPE65 levels and rhodopsin regeneration in rats
  JOURNAL   Exp Eye Res 76 (6), 695-700 (2003)
   PUBMED   12742352
REFERENCE   7  (bases 1 to 2147)
  AUTHORS   Van Hooser,J.P., Liang,Y., Maeda,T., Kuksa,V., Jang,G.F., He,Y.G.,
            Rieke,F., Fong,H.K., Detwiler,P.B. and Palczewski,K.
  TITLE     Recovery of visual functions in a mouse model of Leber congenital
            amaurosis
  JOURNAL   J Biol Chem 277 (21), 19173-19182 (2002)
   PUBMED   11897783
REFERENCE   8  (bases 1 to 2147)
  AUTHORS   Manes,G., Leducq,R., Kucharczak,J., Pages,A., Schmitt-Bernard,C.F.
            and Hamel,C.P.
  TITLE     Rat messenger RNA for the retinal pigment epithelium-specific
            protein RPE65 gradually accumulates in two weeks from late
            embryonic days
  JOURNAL   FEBS Lett 423 (2), 133-137 (1998)
   PUBMED   9512345
REFERENCE   9  (bases 1 to 2147)
  AUTHORS   Bridges,C.D.
  TITLE     Distribution of retinol isomerase in vertebrate eyes and its
            emergence during retinal development
  JOURNAL   Vision Res 29 (12), 1711-1717 (1989)
   PUBMED   2631392
REFERENCE   10 (bases 1 to 2147)
  AUTHORS   Bridges,C.D. and Alvarez,R.A.
  TITLE     The visual cycle operates via an isomerase acting on all-trans
            retinol in the pigment epithelium
  JOURNAL   Science 236 (4809), 1678-1680 (1987)
   PUBMED   3603006
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Mar 29, 2013 this sequence version replaced NM_053562.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.14224.1,
                                           SRR26360194.765986.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                JAXUCZ010000002.1  251425368-251425425
            59-141              JAXUCZ010000002.1  251426486-251426568
            142-292             JAXUCZ010000002.1  251430130-251430280
            293-400             JAXUCZ010000002.1  251440155-251440262
            401-542             JAXUCZ010000002.1  251440354-251440495
            543-690             JAXUCZ010000002.1  251445292-251445439
            691-772             JAXUCZ010000002.1  251446974-251447055
            773-905             JAXUCZ010000002.1  251447261-251447393
            906-1045            JAXUCZ010000002.1  251447489-251447628
            1046-1175           JAXUCZ010000002.1  251448323-251448452
            1176-1290           JAXUCZ010000002.1  251454352-251454466
            1291-1385           JAXUCZ010000002.1  251454554-251454648
            1386-1497           JAXUCZ010000002.1  251454752-251454863
            1498-1660           JAXUCZ010000002.1  251456230-251456392
            1661-2147           JAXUCZ010000002.1  251456670-251457156
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q45"
     gene            1..2147
                     /gene="Rpe65"
                     /note="retinoid isomerohydrolase RPE65"
                     /db_xref="GeneID:89826"
                     /db_xref="RGD:621396"
     exon            1..58
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1649
                     /gene="Rpe65"
                     /EC_number="3.1.1.64"
                     /EC_number="5.3.3.22"
                     /note="retinol isomerase; retinal pigment epithelium 65;
                     lutein isomerase; retinal pigment epithelium-specific
                     protein 65; all-trans-retinyl-palmitate hydrolase; retinal
                     pigment epithelium-specific 65 kDa protein; retinal
                     pigment epithelium 65-like; RPE65, retinoid
                     isomerohydrolase; meso-zeaxanthin isomerase"
                     /codon_start=1
                     /product="retinoid isomerohydrolase"
                     /protein_id="NP_446014.2"
                     /db_xref="GeneID:89826"
                     /db_xref="RGD:621396"
                     /translation="MSIQIEHPAGGYKKLFETVEELSTPLTAHVTGRIPLWLTGSLLR
                     CGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYYRRFIRTDAYVRAMTEKRIVITE
                     FGTCAFPDPCKNIFSRFFSYFRGVEITDNALVNIYPVGEDYYACTETNFITKINPETL
                     ETIKQVDLCNYVSVNGATAHPHIESDGTVYNIGNCFGKNFTVAYNIIKIPPLKADKED
                     PINKSEVVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYM
                     DCFESNESMGVWLHVADKKRRKYFNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGF
                     EFVYNYLYLANLRENWEEVKRNAMKAPQPEVRRYVLPLTIDKADTGRNLVTLPHTTAT
                     AILCSDETIWLEPEVLFSGPRQAFEFPQINYQKCGGKPYTYAYGLGLNHFVPDKLCKL
                     NVKTKEIWMWQEPDSYPSEPIFVSQPDALEEDDGVVLSVVVSPGAGQKPAYLLVLNAK
                     DLSEIARAEVETNIPVTFHGLFKKP"
     exon            59..141
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            142..292
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            293..400
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            401..542
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            543..690
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            691..772
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            773..905
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1045
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1175
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1290
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1385
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1497
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1660
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..2147
                     /gene="Rpe65"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcctacagc tggtaccaga attctctcta gtcttcactg gaagaaaatg tctatccaaa
       61 ttgaacaccc tgctggtggc tacaagaaac tatttgaaac tgtggaggaa ctgtccacac
      121 cactaacagc tcatgtcaca ggcaggattc ccctctggct cactggcagt ctccttcgat
      181 gtgggccagg gctctttgaa gttggatctg agccttttta tcacctgttt gatggacaag
      241 cccttttgca caagtttgac tttaaggagg gccatgtcac atactacagg agattcatcc
      301 gcactgatgc ttatgttcga gcaatgaccg agaagaggat tgtcataaca gaatttggca
      361 cctgtgcttt tccagacccc tgcaagaata tattttccag gtttttttct tactttcgag
      421 gagtagagat tactgacaat gcccttgtaa atatttaccc agtgggagaa gattactatg
      481 catgcacaga gaccaacttt atcacaaaga ttaacccaga gaccttggag actattaagc
      541 aggttgatct ttgcaactat gtttccgtca atggtgccac tgctcatcca catattgaaa
      601 gtgatggaac agtttataac attggcaatt gctttgggaa aaattttaca gttgcctaca
      661 acattattaa gatccctcca ctgaaagcag acaaggaaga cccaataaac aagtcagaag
      721 ttgttgtgca gttcccatgc agtgatcggt tcaagccatc ttatgtacac agttttggtc
      781 tgactcccaa ctatatcgtt tttgtggaga ctccagtcaa aattaacctt ttcaagtttc
      841 tttcttcgtg gagtctttgg ggagccaact acatggactg tttcgagtcc aatgaaagca
      901 tgggggtttg gcttcatgtt gctgacaaaa aaagaagaaa atatttcaat aacaaataca
      961 ggacctcccc tttcaatctc ttccatcata tcaatactta tgaagataat gggtttctga
     1021 ttgtggatct ctgttgctgg aaagggtttg aatttgttta taattactta tatttagcta
     1081 atttacgtga gaattgggaa gaagtaaaac gaaatgctat gaaagctcct cagcctgaag
     1141 tcaggagata cgttcttcct ttgacaattg acaaggctga cacaggcaga aatttagtca
     1201 cacttcccca tacaactgcc acagccattc tgtgcagtga tgagaccata tggctggaac
     1261 ctgaagtcct cttttcaggg ccccgtcaag cctttgaatt tcctcaaatc aattaccaga
     1321 aatgtggggg gaaaccttac acttatgcat acggacttgg attgaatcac tttgttccag
     1381 acaagttgtg taagctgaat gtcaaaacta aagaaatctg gatgtggcaa gaaccggatt
     1441 cttacccatc tgaacccatc tttgtttctc aaccggatgc tctggaagaa gatgatggtg
     1501 tagttctgag tgtggtggtg agtcctgggg caggacaaaa gcctgcatat ctcctggttc
     1561 tgaatgccaa agacttgagt gaaattgcca gggctgaagt ggagactaat attcctgtga
     1621 ccttccatgg actgttcaaa aaaccatgaa catattccag ggactggaga gaagactcag
     1681 cagttaagag cactgactgc tcttcgagag atcctgagtt cagttcacaa cgaccaaatg
     1741 gtggctcaca accatctgta atgggatcca atgtcctctt ctgatgtatc tgaagatagc
     1801 tacagtgtgt atgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa
     1861 aataaatctt aaaaaaaaca gaaaaaataa aataattctg tttattttag cttgctgtct
     1921 atcatggttt taagtcaaag atgtacacaa ttttgcactg tttttcagaa atttctgagt
     1981 tggacaagta gtttctttaa aaattaagtg attatttata tgatgatact ttctctgtga
     2041 gataaaaata aactgaaaaa ttattttaaa cacttagtaa tcaaatatga aaggaatgta
     2101 gactttgaaa agctgccttt tctataataa aagttgtttt tagaaag
//
