LOCUS       NM_001389239            6540 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus Rho-associated coiled-coil containing protein
            kinase 1 (Rock1), transcript variant 1, mRNA.
ACCESSION   NM_001389239 XM_006254400
VERSION     NM_001389239.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Fu,Q., Deng,Y., Zhou,B., Lei,J., Peng,K. and Feng,C.
  TITLE     miR-488-3p alleviates neuropathic pain by regulating target gene
            ROCK1
  JOURNAL   Acta Neurobiol Exp (Wars) 83 (3), 271-279 (2023)
   PUBMED   37874190
  REMARK    GeneRIF: miR-488-3p alleviates neuropathic pain by regulating
            target gene ROCK1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Zhou,W., Yuan,X., Li,J., Wang,W. and Ye,S.
  TITLE     Retinol binding protein 4 promotes the phenotypic transformation of
            vascular smooth muscle cells under high glucose condition via
            modulating RhoA/ROCK1 pathway
  JOURNAL   Transl Res 259, 13-27 (2023)
   PUBMED   37003483
  REMARK    GeneRIF: Retinol binding protein 4 promotes the phenotypic
            transformation of vascular smooth muscle cells under high glucose
            condition via modulating RhoA/ROCK1 pathway.
REFERENCE   3  (bases 1 to 6540)
  AUTHORS   Wang,H., Wang,X., Liu,X., Zhou,J., Yang,Q., Chai,B., Chai,Y., Ma,Z.
            and Lu,S.
  TITLE     miR-199a-5p Plays a Pivotal Role on Wound Healing via Suppressing
            VEGFA and ROCK1 in Diabetic Ulcer Foot
  JOURNAL   Oxid Med Cell Longev 2022, 4791059 (2022)
   PUBMED   35432725
  REMARK    GeneRIF: miR-199a-5p Plays a Pivotal Role on Wound Healing via
            Suppressing VEGFA and ROCK1 in Diabetic Ulcer Foot.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6540)
  AUTHORS   Wei,Z., Xue,Y., Xue,Y., Cheng,J., Lv,G., Chu,L., Ma,Z. and Guan,S.
  TITLE     Ferulic acid attenuates non-alcoholic steatohepatitis by reducing
            oxidative stress and inflammation through inhibition of the
            ROCK/NF-kappaB signaling pathways
  JOURNAL   J Pharmacol Sci 147 (1), 72-80 (2021)
   PUBMED   34294375
  REMARK    GeneRIF: Ferulic acid attenuates non-alcoholic steatohepatitis by
            reducing oxidative stress and inflammation through inhibition of
            the ROCK/NF-kappaB signaling pathways.
REFERENCE   5  (bases 1 to 6540)
  AUTHORS   Shi,X.J., Jin,Y., Xu,W.M., Shen,Q., Li,J. and Chen,K.
  TITLE     MicroRNA-23a reduces lipopolysaccharide-induced cellular apoptosis
            and inflammatory cytokine production through Rho-associated kinase
            1/sirtuin-1/nuclear factor-kappa B crosstalk
  JOURNAL   Chin Med J (Engl) 134 (7), 829-839 (2021)
   PUBMED   33538509
  REMARK    GeneRIF: MicroRNA-23a reduces lipopolysaccharide-induced cellular
            apoptosis and inflammatory cytokine production through
            Rho-associated kinase 1/sirtuin-1/nuclear factor-kappa B crosstalk.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6540)
  AUTHORS   Yanazume,T., Hasegawa,K., Wada,H., Morimoto,T., Abe,M., Kawamura,T.
            and Sasayama,S.
  TITLE     Rho/ROCK pathway contributes to the activation of extracellular
            signal-regulated kinase/GATA-4 during myocardial cell hypertrophy
  JOURNAL   J Biol Chem 277 (10), 8618-8625 (2002)
   PUBMED   11739382
  REMARK    GeneRIF: the Rho/ROCK pathway is linked to downstream GATA-4 via
            the activation of extracellular signal-regulated kinases during
            myocardial cell hypertrophy
REFERENCE   7  (bases 1 to 6540)
  AUTHORS   Sumi,T., Matsumoto,K. and Nakamura,T.
  TITLE     Specific activation of LIM kinase 2 via phosphorylation of
            threonine 505 by ROCK, a Rho-dependent protein kinase
  JOURNAL   J Biol Chem 276 (1), 670-676 (2001)
   PUBMED   11018042
REFERENCE   8  (bases 1 to 6540)
  AUTHORS   Ohashi,K., Nagata,K., Maekawa,M., Ishizaki,T., Narumiya,S. and
            Mizuno,K.
  TITLE     Rho-associated kinase ROCK activates LIM-kinase 1 by
            phosphorylation at threonine 508 within the activation loop
  JOURNAL   J Biol Chem 275 (5), 3577-3582 (2000)
   PUBMED   10652353
REFERENCE   9  (bases 1 to 6540)
  AUTHORS   Uehata,M., Ishizaki,T., Satoh,H., Ono,T., Kawahara,T.,
            Morishita,T., Tamakawa,H., Yamagami,K., Inui,J., Maekawa,M. and
            Narumiya,S.
  TITLE     Calcium sensitization of smooth muscle mediated by a Rho-associated
            protein kinase in hypertension
  JOURNAL   Nature 389 (6654), 990-994 (1997)
   PUBMED   9353125
REFERENCE   10 (bases 1 to 6540)
  AUTHORS   Leung,T., Chen,X.Q., Manser,E. and Lim,L.
  TITLE     The p160 RhoA-binding kinase ROK alpha is a member of a kinase
            family and is involved in the reorganization of the cytoskeleton
  JOURNAL   Mol Cell Biol 16 (10), 5313-5327 (1996)
   PUBMED   8816443
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On Dec 1, 2020 this sequence version replaced XM_006254400.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-954               JAXUCZ010000018.1  1508194-1509147     c
            955-1036            JAXUCZ010000018.1  1472899-1472980     c
            1037-1137           JAXUCZ010000018.1  1464541-1464641     c
            1138-1275           JAXUCZ010000018.1  1463899-1464036     c
            1276-1451           JAXUCZ010000018.1  1462413-1462588     c
            1452-1536           JAXUCZ010000018.1  1460848-1460932     c
            1537-1681           JAXUCZ010000018.1  1458728-1458872     c
            1682-1820           JAXUCZ010000018.1  1457893-1458031     c
            1821-1912           JAXUCZ010000018.1  1455731-1455822     c
            1913-2072           JAXUCZ010000018.1  1439092-1439251     c
            2073-2133           JAXUCZ010000018.1  1435877-1435937     c
            2134-2222           JAXUCZ010000018.1  1432698-1432786     c
            2223-2271           JAXUCZ010000018.1  1428691-1428739     c
            2272-2407           JAXUCZ010000018.1  1423318-1423453     c
            2408-2499           JAXUCZ010000018.1  1421599-1421690     c
            2500-2746           JAXUCZ010000018.1  1421264-1421510     c
            2747-2853           JAXUCZ010000018.1  1416561-1416667     c
            2854-3004           JAXUCZ010000018.1  1415252-1415402     c
            3005-3165           JAXUCZ010000018.1  1413209-1413369     c
            3166-3350           JAXUCZ010000018.1  1411366-1411550     c
            3351-3420           JAXUCZ010000018.1  1408982-1409051     c
            3421-3515           JAXUCZ010000018.1  1407946-1408040     c
            3516-3681           JAXUCZ010000018.1  1406646-1406811     c
            3682-3775           JAXUCZ010000018.1  1404650-1404743     c
            3776-3864           JAXUCZ010000018.1  1403516-1403604     c
            3865-4053           JAXUCZ010000018.1  1400678-1400866     c
            4054-4213           JAXUCZ010000018.1  1399520-1399679     c
            4214-4284           JAXUCZ010000018.1  1394749-1394819     c
            4285-4373           JAXUCZ010000018.1  1394460-1394548     c
            4374-4452           JAXUCZ010000018.1  1391841-1391919     c
            4453-4714           JAXUCZ010000018.1  1391441-1391702     c
            4715-4922           JAXUCZ010000018.1  1390354-1390561     c
            4923-6540           JAXUCZ010000018.1  1387360-1388977     c
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p13"
     gene            1..6540
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /note="Rho-associated coiled-coil containing protein
                     kinase 1"
                     /db_xref="GeneID:81762"
                     /db_xref="RGD:620424"
     exon            1..954
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     CDS             862..4926
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /EC_number="2.7.11.1"
                     /note="p160 ROCK-1; p150 RhoA-binding kinase ROK beta;
                     liver regeneration-related protein LRRG199;
                     rho-associated, coiled-coil-containing protein kinase 1;
                     Rho-associated kinase beta; Rho-associated coiled-coil
                     forming kinase 1; rho-associated, coiled-coil-containing
                     protein kinase I"
                     /codon_start=1
                     /product="rho-associated protein kinase 1"
                     /protein_id="NP_001376168.1"
                     /db_xref="GeneID:81762"
                     /db_xref="RGD:620424"
                     /translation="MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFP
                     ALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVY
                     AMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGD
                     LVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFG
                     TCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDT
                     PFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKR
                     HLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQL
                     PFVGFTYYSNRRYLPSANPSENRSSSNVDKNVQESLQKTIYKLEEQLHNEMQLKDEME
                     QKCRTSNIKLDKIMKELDEEGNQRRNLESAVSQIEKEKMLLQHRINEYQRKVEQENEK
                     RRNVENEVSTLKDQLEDLRKASQSSQLANEKLTQLQKQLEEANDLLRTESDTAVRLRK
                     SHTEMSKSVSQLESLNRELQERNRMLENSKSQADKDYYQLQAVLEAERRDRGHDSEMI
                     GDLQARITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSIQQ
                     RLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVETEKQ
                     CSMLDVDLKQSQQKLEHLTENKERLEDAVKSLTLQLEQESNKRILLQSELKTQAFEAD
                     NLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLY
                     KTQVKELKEEIEEKNRENLRKIQELQSEKETLSTQLDLAETKAESEQLARGILEEQYF
                     ELTQESKKAASRNRQEITDKDHTVSRLEEANNALTKDIELLRKENEELNERMRTAEEE
                     YKLKKEEEISNLKAAFEKNISTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKK
                     EKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECTHRNELQMQLASKESDIE
                     QLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVS
                     SKKMLFYNDEQDKEQSSPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEG
                     ECRKDIEVEPVQQGEKTNFQNHKGHEFIPTLYHFPANCEACAKPLWHVFKPPPALECR
                     RCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNP
                     PSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS"
     exon            955..1036
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1137
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1275
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1451
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1536
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1681
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1820
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1912
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2072
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2133
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2222
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2271
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2407
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2499
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2746
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2853
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..3004
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3165
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..3350
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3420
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3515
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3516..3681
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3682..3775
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3864
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4053
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4054..4213
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4214..4284
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4373
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4374..4452
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4453..4714
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4715..4922
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
     exon            4923..6540
                     /gene="Rock1"
                     /gene_synonym="P160Rock; ROCK-I"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tttgaacagg aaggcggaca tattagtccc tctgagccct cctcgcccca ccccccaggc
       61 attcgcctcc gcgactcgcc cttttcccag ctcggaccgc ggcccctccc agaagctccc
      121 gcatcagcag ccgcacggac cctctggtcg ccttcctcat cggcccgctc cagctagtct
      181 tcctctgaga ctccctcggg ccaccgacct caccctgccc ggccggacac cgtaacccca
      241 gcagcgaccg gataaaatgg gagagtgagc ctgttctgtg gaaaccagct cttggccgag
      301 gataaatcag tgcgtcttac cgggccgagt ggaacccggg atgcagggct agacagccgc
      361 tagcgcctta gagcggagcg gggccggccc ggggcccagg cggcagctgg cgcagctcct
      421 cacccgccct tcgctttcgc ctttcctctt cgccctccct ggctgccccg agggagtctc
      481 caccctgctg cgctccctct tcccgccgct gcccttctct ggactgggcc ccgccatggc
      541 gaaggcagcc ggggcccgct aggctgaggt gcctcgccgg agcgaccggg ctgctggcga
      601 cggcgctgtc ggctgtcgcg aggggctgcc gggtgggatg cgactttggg tgtccgagcg
      661 gctgcgggtc gctgtcaccc ccagcctggg gtctagagag cagaggtccc ctcagtgagg
      721 ggaaggaggg ggaaccgggc gcacctggtg accccgaggt tccggctcct ctgccccgcg
      781 gctacaaacc caccgcggag gaagttggtt gaacttgctt tccgctgcgg gtacgaaggt
      841 atcgtcacaa gtagcagcat catgtcgact ggggacagtt ttgagactcg gtttgaaaaa
      901 atcgacaacc tgctgcggga tcccaaatcg gaagtgaatt cagattgttt gctggatgga
      961 ttggatgctt tggtttatga tttggatttt cctgccttaa gaaaaaacaa aaatattgac
     1021 aactttctaa gcagatataa agacacaata aataaaatta gagatttacg gatgaaagct
     1081 gaagattatg aagtagtaaa ggtaatcggc agaggtgcat ttggagaagt tcaattggta
     1141 aggcataaat ccaccaggaa ggtttatgct atgaagcttc tcagcaaatt tgaaatgatt
     1201 aagagatctg attctgcttt tttctgggaa gaaagggaca tcatggcatt tgccaatagt
     1261 ccttgggttg ttcagctttt ttatgcattc caagatgacc gatatctcta tatggtgatg
     1321 gagtacatgc ctggtggaga ccttgtaaac ctgatgagca actacgatgt gcctgaaaag
     1381 tgggcacgat tctatactgc agaagtagtt ctcgcattgg atgcaatcca ttccatgggt
     1441 tttattcata gagatgtgaa gcctgacaac atgctgctgg ataagtctgg acatttgaag
     1501 ttagccgact ttggtacttg tatgaagatg aataaggaag gtatggtacg atgtgataca
     1561 gcagttggaa cacctgatta tatttcacct gaagtattaa aatcacaagg cggtgatggc
     1621 tattatggac gagagtgtga ctggtggtca gttggtgtat ttttatatga aatgcttgta
     1681 ggtgatacac ctttttatgc agattctttg gttgggacgt acagtaaaat tatgaaccat
     1741 aaaaattcac ttactttccc tgatgataat gacatatcaa aagaggcgaa aaatctcatt
     1801 tgtgccttcc ttacggacag agaagtgaga ttgggcagaa atggtgtaga ggaaatcaaa
     1861 cgtcatctct tcttcaaaaa tgatcagtgg gcttgggaaa cgctccgaga cactgtagca
     1921 ccagttgtgc ctgatttaag tagtgacatt gatactagta attttgatga cttagaagaa
     1981 gataaaggag atgaagaaac atttcctatt ccaaaagctt ttgttggcaa tcagctacct
     2041 tttgtaggat ttacatatta tagcaatcgt agatacttac cttcagcaaa tcccagtgag
     2101 aacagatcta gctccaatgt ggataaaaac gtgcaggaaa gtttacagaa aacaatctac
     2161 aagcttgaag aacaactgca taatgaaatg caattaaaag atgaaatgga gcaaaagtgc
     2221 agaacttcaa acataaaact agacaagata atgaaagaat tggatgaaga ggggaatcaa
     2281 agaagaaatc tagaatctgc ggtgtctcaa attgagaaag aaaagatgtt gctacaacat
     2341 agaattaatg agtaccaaag aaaagtggaa caggaaaatg agaaaagaag aaatgtagaa
     2401 aatgaagttt ctacactaaa ggaccagttg gaagacttac ggaaagcaag tcagagctca
     2461 cagcttgcta atgagaagct gacacagtta cagaagcagt tagaagaagc gaatgactta
     2521 cttaggacag aatcagacac agctgtaaga ttaaggaaga gtcacacaga gatgagcaag
     2581 tcagttagtc agctggagtc tctgaacaga gagttacagg agagaaacag aatgttagag
     2641 aattccaagt cacaagcaga caaggattac taccagctgc aagctgtatt ggaagctgag
     2701 cgaagagaca gaggccatga ttctgagatg attggagacc ttcaagcacg aattacgtct
     2761 ttacaagaag aggtgaagca tctcaaacat aatcttgaaa gagtagaagg agaaagaaaa
     2821 gaggctcaag acatgctcaa tcattcagaa aaggaaaaaa ataatttgga gattgatttg
     2881 aactacaagc tcaagtctat tcagcagagg ttagaacaag aagtaaatga acacaaagta
     2941 accaaagcgc ggctaactga caagcatcag tctattgaag aggcaaagtc tgttgccatg
     3001 tgtgaaatgg aaaaaaagct gaaggaagag agagaagctc gagagaaggc tgaaaataga
     3061 gttgttgaga ctgagaagca atgttctatg ctagatgttg acctgaaaca gtctcagcag
     3121 aagctagaac atttgaccga aaataaagaa aggctggagg atgcagttaa gagtctcacc
     3181 ctgcagctgg aacaggaatc caataagcgg atcttgctac agagtgaact gaagactcaa
     3241 gcgtttgaag cagacaattt aaaagggtta gaaaaacaga tgaaacagga aataaatact
     3301 ttattggaag ctaaaagatt gttagaattt gagttagctc agcttacaaa gcaatacaga
     3361 ggaaatgaag ggcaaatgcg ggagttacaa gatcagcttg aagctgagca atatttctct
     3421 acactttata aaacccaggt aaaggaactt aaagaagaaa ttgaagaaaa aaacagagaa
     3481 aatttaagga aaatacagga gctgcaaagt gaaaaagaaa ctctttcgac tcagttggat
     3541 ttagcagaaa caaaagctga gtctgagcag ttggctcgag gaattctgga agaacagtat
     3601 tttgagttga ctcaagaaag caagaaagct gcttcaagaa atagacaaga gattacagat
     3661 aaagatcata ccgttagtcg gcttgaagaa gcaaacaatg cgctaaccaa agatattgaa
     3721 ctactaagaa aagaaaatga agagctaaat gaaaggatga gaacagcaga agaagaatat
     3781 aaattgaaga aggaggaaga aataagtaat cttaaggctg cctttgaaaa gaatatcagt
     3841 accgaacgga cccttaaaac acaggctgtt aacaagttgg cagaaataat gaatcgcaaa
     3901 gattttaaaa ttgataggaa gaaagctaat actcaagatt tgagaaaaaa agaaaaggaa
     3961 aatagaaagc tccaactgga acttaaccaa gaaagagaaa aatttaacca aatggtggtg
     4021 aaacaccaga aggagctgaa tgacatgcaa gcgcaattgg tagaagaatg tacacataga
     4081 aatgaacttc aaatgcagtt ggctagcaaa gagagtgata ttgagcagtt gcgtgcaaaa
     4141 cttttggacc tttcggattc aactagtgtt gctagttttc ccagtgctga tgagactgac
     4201 ggaaatcttc cagagtcaag aattgaaggt tggctttctg taccaaatag aggaaatatc
     4261 aaaagatatg gctggaagaa acagtatgtt gtggtgagca gcaaaaaaat gttgttctat
     4321 aatgatgagc aagataagga acaatccagt ccatctatgg tgttggacat agataaattg
     4381 ttccatgtcc gacctgtaac ccaaggagat gtgtacagag ctgaaactga agaaattcct
     4441 aaaatattcc agatactgta tgcaaatgaa ggtgaatgta gaaaagatat agaagtggaa
     4501 ccagttcaac aaggtgaaaa aactaatttc cagaatcaca aaggccatga attcattcct
     4561 accctctacc actttcctgc caactgcgag gcctgtgcca aacctttgtg gcatgttttt
     4621 aagccaccgc ctgccctaga gtgtcgaaga tgccatgtta agtgccacag ggaccatcta
     4681 gacaagaaag aggacttgat ttccccatgc aaagtaagtt atgatgtaac atcagcaaga
     4741 gatatgttgc tgttagcatg ttctcaggat gaacaaaaga aatgggtaac tcatctggta
     4801 aagaagattc ctaagaaccc accttctggc tttgtccgtg cctctcctcg aacgctgtcg
     4861 acaagatcta ctgcaaatca gtctttccgg aaagtagtca aaaatacatc tggaaaaact
     4921 agttaaccat gtgacctact gctgtgttgg aatcatatgg gatgctacct gatcaaccag
     4981 gctgtttaac catgtggaac agacaggctg tttcttttga cacaaatacc acaggctttt
     5041 cagggttaag attgctgttg ttctgtcctt gttttggcac aaaaagcaca ctgaggattg
     5101 tttttgtttt cttgcgggtt tgcctacagg tagtttagat taattattac tatgcaatgc
     5161 aagtacaatt ggggaaagct ttgctaggtt tttttttttt aaggtgctgc ctttttggat
     5221 ttataaggaa atgcctgtca gtcatgatag aacagttttc ctcatatgaa caagaggaaa
     5281 gaactttcac tttcagtgga atggccatca ctatcaagat cagatcgtgg aagaagtaaa
     5341 gaaaatatct caaaatgaga caagtttttc tgttttgttg ttcaataact aagttttttg
     5401 tgctcttgca agactatacg aactatttta agaacacagt gatgtcactt gaagctcagt
     5461 gtccttactg tagaattaaa agccttactg tcaattgccc acagtgggct ctatggagaa
     5521 attaaatatc atacattatg ctttacaaaa tattgtatat gtttcagcaa gtttgtagat
     5581 tgggaactgg gagaagatga aagaattaca tttaatctat gcatttttgc caagccatat
     5641 tgagatattt tactactaaa gacataaaga actaactgta caagagaacc atgtttaaaa
     5701 gccttttgtg gggtacatca tttctgtaat tatataatgt atttctttgt ggttttaagt
     5761 aaaaagacat taagttaaca aacataaaaa tgtatgcact gtttgaaatg taaattattc
     5821 ttagaacact ttcagtggga gttgcattac cctattagtg ccttaatttg agagaatttt
     5881 tttactgcca taagtacaga cttttcagaa aacagatttt acaaaaatta tgttttttca
     5941 ttggtaattg gtttaattta aaatatagtt tgtcagactt acataaattg agtacaacct
     6001 ttgtaccaaa ctatctgcta caataatgaa actatctgca aaaacataca gttgcaccaa
     6061 tagtaaagaa ttatgaaata tgtccatgat gttttccatt taacatagga attaccatat
     6121 aagtactgtt cgactctctg ttcagctgta acaggagttg atgtgtatgg gtggtgtgat
     6181 ttattgttgt ttttaaccaa atacctcctc agtaatttat aacggctttg cagtaatgtg
     6241 tatcaagtaa gcactggaaa accgatcttc tctagggtga catgcatgtt tcaagtggta
     6301 ttgaaagccg cactgatgga tatgtaataa taaacatatc tgttattaat atgctaatgg
     6361 ctctgtgctc atttaatgag aaatgaaata atttatggat aaatactttt aatattgact
     6421 tactatggta ttttctcatg gctggaatga atagatggca tactaaaaat tattcagtga
     6481 ttgtatacaa atgtttgcta aattttgtaa atagattaaa attatttttt aaagataaaa
//
