LOCUS       NM_001305215            5019 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus roundabout guidance receptor 4 (Robo4), mRNA.
ACCESSION   NM_001305215 XM_006242819
VERSION     NM_001305215.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Shuwen,H., Xi,Y., Miao,D., Jiamin,X., Jing,Z. and Weili,G.
  TITLE     Nine Genes Mediate the Therapeutic Effects of Iodine-131
            Radiotherapy in Thyroid Carcinoma Patients
  JOURNAL   Dis Markers 2020, 9369341 (2020)
   PUBMED   32626543
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Gould,R.A., Aziz,H., Woods,C.E., Seman-Senderos,M.A., Sparks,E.,
            Preuss,C., Wunnemann,F., Bedja,D., Moats,C.R., McClymont,S.A.,
            Rose,R., Sobreira,N., Ling,H., MacCarrick,G., Kumar,A.A.,
            Luyckx,I., Cannaerts,E., Verstraeten,A., Bjork,H.M., Lehsau,A.C.,
            Jaskula-Ranga,V., Lauridsen,H., Shah,A.A., Bennett,C.L.,
            Ellinor,P.T., Lin,H., Isselbacher,E.M., Lino Cardenas,C.L.,
            Butcher,J.T., Hughes,G.C., Lindsay,M.E., Mertens,L.,
            Franco-Cereceda,A., Verhagen,J.M.A., Wessels,M., Mohamed,S.A.,
            Eriksson,P., Mital,S., Van Laer,L., Loeys,B.L., Andelfinger,G.,
            McCallion,A.S. and Dietz,H.C.
  CONSRTM   Baylor-Hopkins Center for Mendelian Genomics; MIBAVA Leducq
            Consortium
  TITLE     ROBO4 variants predispose individuals to bicuspid aortic valve and
            thoracic aortic aneurysm
  JOURNAL   Nat Genet 51 (1), 42-50 (2019)
   PUBMED   30455415
REFERENCE   3  (bases 1 to 5019)
  AUTHORS   Jin,X., Shin,Y.J., Riew,T.R., Choi,J.H. and Lee,M.Y.
  TITLE     Increased Expression of Slit2 and its Robo Receptors During
            Astroglial Scar Formation After Transient Focal Cerebral Ischemia
            in Rats
  JOURNAL   Neurochem Res 41 (12), 3373-3385 (2016)
   PUBMED   27686659
  REMARK    GeneRIF: data revealed a selective and sustained induction of Slit2
            and Robo in astrocytes localized throughout the astroglial scar
            after ischemic stroke, suggesting that Slit2/Robo signaling
            participates in glial scar formation and brain remodeling following
            ischemic injury.
REFERENCE   4  (bases 1 to 5019)
  AUTHORS   Park,J.H., Pak,H.J., Riew,T.R., Shin,Y.J. and Lee,M.Y.
  TITLE     Increased expression of Slit2 and its receptors Robo1 and Robo4 in
            reactive astrocytes of the rat hippocampus after transient
            forebrain ischemia
  JOURNAL   Brain Res 1634, 45-56 (2016)
   PUBMED   26764532
  REMARK    GeneRIF: Results suggest that Slit2/Robo signaling may be involved
            in regulating the astroglial reaction via autocrine or paracrine
            mechanisms in post-ischemic processes.
REFERENCE   5  (bases 1 to 5019)
  AUTHORS   Zheng,W., Geng,A.Q., Li,P.F., Wang,Y. and Yuan,X.B.
  TITLE     Robo4 regulates the radial migration of newborn neurons in
            developing neocortex
  JOURNAL   Cereb Cortex 22 (11), 2587-2601 (2012)
   PUBMED   22123939
  REMARK    GeneRIF: our results showed an important role of Robo4 in the
            regulation of cortical radial migration through Slit-dependent and
            -independent mechanisms
REFERENCE   6  (bases 1 to 5019)
  AUTHORS   Liao,W.X., Laurent,L.C., Agent,S., Hodges,J. and Chen,D.B.
  TITLE     Human placental expression of SLIT/ROBO signaling cues: effects of
            preeclampsia and hypoxia
  JOURNAL   Biol Reprod 86 (4), 111 (2012)
   PUBMED   22262697
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5019)
  AUTHORS   Bedell,V.M., Yeo,S.Y., Park,K.W., Chung,J., Seth,P.,
            Shivalingappa,V., Zhao,J., Obara,T., Sukhatme,V.P., Drummond,I.A.,
            Li,D.Y. and Ramchandran,R.
  TITLE     roundabout4 is essential for angiogenesis in vivo
  JOURNAL   Proc Natl Acad Sci U S A 102 (18), 6373-6378 (2005)
   PUBMED   15849270
REFERENCE   8  (bases 1 to 5019)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
REFERENCE   9  (bases 1 to 5019)
  AUTHORS   Park,K.W., Morrison,C.M., Sorensen,L.K., Jones,C.A., Rao,Y.,
            Chien,C.B., Wu,J.Y., Urness,L.D. and Li,D.Y.
  TITLE     Robo4 is a vascular-specific receptor that inhibits endothelial
            migration
  JOURNAL   Dev Biol 261 (1), 251-267 (2003)
   PUBMED   12941633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Aug 31, 2022 this sequence version replaced NM_001305215.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643285.30668.1,
                                           SRR26643290.28827.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               JAXUCZ010000008.1  45308739-45308918
            181-513             JAXUCZ010000008.1  45309297-45309629
            514-671             JAXUCZ010000008.1  45309869-45310026
            672-792             JAXUCZ010000008.1  45310222-45310342
            793-920             JAXUCZ010000008.1  45310848-45310975
            921-1149            JAXUCZ010000008.1  45311055-45311283
            1150-1262           JAXUCZ010000008.1  45311525-45311637
            1263-1431           JAXUCZ010000008.1  45312289-45312457
            1432-1617           JAXUCZ010000008.1  45312641-45312826
            1618-1660           JAXUCZ010000008.1  45312951-45312993
            1661-1797           JAXUCZ010000008.1  45314813-45314949
            1798-2061           JAXUCZ010000008.1  45315045-45315308
            2062-2169           JAXUCZ010000008.1  45317472-45317579
            2170-2329           JAXUCZ010000008.1  45317707-45317866
            2330-2569           JAXUCZ010000008.1  45318005-45318244
            2570-2928           JAXUCZ010000008.1  45318373-45318731
            2929-3135           JAXUCZ010000008.1  45320323-45320529
            3136-5019           JAXUCZ010000008.1  45320639-45322522
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..5019
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /note="roundabout guidance receptor 4"
                     /db_xref="GeneID:100911068"
                     /db_xref="RGD:6494870"
     exon            1..180
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..3146
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /note="roundabout homolog 4-like; roundabout, axon
                     guidance receptor, homolog 4; Magic roundabout"
                     /codon_start=1
                     /product="roundabout homolog 4 precursor"
                     /protein_id="NP_001292144.1"
                     /db_xref="GeneID:100911068"
                     /db_xref="RGD:6494870"
                     /translation="MGQGEELRAAVDSGGMGLLGTKCPLPLLLLFIMGGKALDSPPQI
                     LVHPQDQLLQGSGPAKMSCRASGQPLPTIRWLLNGQPLSMATPDLHYLQSDGTLLLHR
                     PPTHGRPQDDQNILSAILGVYTCEASNRLGTAVSRGARLSVAVLQEDFRIQPRDTVAV
                     VGESLVLECGPPWGYPKPSVSWWKDGKPLVLQPGKRTVSGDSLMVARAEKNDTGTYMC
                     MATNNAGQRESRAARVSIQESPDHKEHLELLAVRIQLENVTLLNPEPVKGPKPGPAVW
                     LSWKVSGPAAPAQSYTALFRAQRDPRDQGSPWTEVLLDGLLNAKLGGLRWGQDYEFKV
                     RPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLRPGNGSVFVSWAPPPAENHNGFIRG
                     YQVWSLGNASLPAANWTVVGEQTQLEIAARMPGSYCVQVAAVTGAGAGEPSIPVCLLL
                     EQAMEQSARDPSKHVSWTLEQLRATLKRPEVIASGAVLLWLLLLGIAVCIYRRRKAGV
                     HLGPGLYRYTSEDAILKHRMDHSDSPWLADTWRSTSGSRDLSSSSSLSSRLGVDPRDP
                     LDGRRSLISWDPRSPGVPLLPDTSTFYGSLIAEQTSSPPVRPSPQTPAARRLPPKLTG
                     TSSPWASSDSLCSRRGLCSPRMSLAPAEAWKAKKKQELHQANSSPLLRGSHPMEIWAW
                     ELGSRASKNLSQSPGPNTCSPREAPGAVVAWRALGPQLHRNSSELAARPLPPTPLSLR
                     GAPSHDPQSQCVEKLQAPSSDPLPAAPLSVLNSSRPSSPQASFLSVPSPGSSNLSSSS
                     LSSLEEEDQDSVLTPEEVALCLELSDGEETPTNSVSPMPRAPSPPATYGYISIPTSSG
                     LADMGRAGGGVGSEVGNLLCPPRLCPTPTPSEGSLANGWGSASEDNVPSARASLVSSS
                     DGSFLADAHFARALAVAVDSFGFSLEPREADCVFTDASSPPSPRDDLSLTRSFSLPLW
                     EWRPDWLEDAEINHTQRLGRGLPPWPPDSRVSSQRSWLTGVVPKAGDSS"
     exon            181..513
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            514..671
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            672..792
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            793..920
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1149
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1262
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1431
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1617
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1660
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1797
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..2061
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2169
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2329
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2569
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2928
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3135
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..5019
                     /gene="Robo4"
                     /gene_synonym="Robo4l"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcacaaac aactaccacc accccacccc tccccttctt cccttactgc agtgagctca
       61 gaacaacagg acaaaagtgt atgggacaag gagaggagct gagagcagcc gttgactctg
      121 gaggaatggg cctcctgggg actaagtgtc ctctgcctct actgcttctt ttcatcatgg
      181 gaggcaaggc tctggattct ccaccccaga tcctagttca tccccaggac cagctacttc
      241 agggctccgg gccggccaag atgagttgca gagcatcggg ccaaccactt cccactatcc
      301 gctggctgct gaatgggcag cccctcagca tggcgacccc agacctacat tacctccaat
      361 cagatgggac cctcctgcta catcggcccc ctacccatgg acggccgcaa gacgaccaga
      421 acattctctc agcaatcctg ggtgtctaca catgtgaggc cagcaaccgg ctgggcacag
      481 cagtgagccg gggtgctagg ctgtctgtgg ctgtcctcca ggaggacttc cggatccaac
      541 ctcgggacac agtggccgtg gtgggcgaga gcttggttct cgagtgtggt cctccctggg
      601 gctacccaaa gccttcagtc tcctggtgga aagatgggaa acccctggtc ctccagccag
      661 ggaagcgcac agtgtctggg gattctctga tggtggcaag agcagagaag aatgacacgg
      721 ggacctatat gtgtatggcc accaacaatg ccggacaacg ggagagtcgg gcagccaggg
      781 tgtctatcca ggagtcaccg gaccacaagg agcatctaga gcttctggct gttcgaattc
      841 agctggaaaa tgtgaccctg ctgaacccag aacctgtaaa aggccccaag cctgggccag
      901 ctgtgtggct cagctggaag gtgagcggcc ctgctgcacc tgcccagtca tacacagccc
      961 tgttcagggc gcagagggac cccagggacc agggatctcc atggacagag gtgctgctgg
     1021 atggcttgct gaatgcaaag cttgggggtc tccgctgggg ccaagactac gaattcaaag
     1081 tgagaccgtc ctccggccgg gctcgaggcc ctgatagcaa tgtgttgctc ctgaggctgc
     1141 ctgaacaggt gcccagtgcc ccacctcaag aagtgaccct aagacctggc aacggtagtg
     1201 tctttgtgag ttgggcccca cctcctgctg aaaaccataa cggtttcatc cgtggctacc
     1261 aggtctggag cctgggcaat gcctcattgc ctgctgccaa ctggaccgta gtgggtgaac
     1321 agacacagct ggagattgcc gcacgaatgc caggctccta ttgtgtgcag gtggctgcag
     1381 tcactggtgc tggggctgga gagcccagta tccctgtctg cctcctttta gagcaggcca
     1441 tggagcaatc agcacgagac cccagtaaac atgtttcctg gaccctggaa cagctgaggg
     1501 ctaccttgaa acgaccagaa gtcattgcca gtggtgccgt cctgctctgg ttgctgctcc
     1561 taggcattgc tgtgtgtatc tacaggcgac ggaaagctgg ggtgcacctg ggcccaggtc
     1621 tctacagata caccagtgag gatgccattt taaaacacag gatggaccac agtgactccc
     1681 catggctggc agacacctgg cgctccacct ctggctctag agatctgagc agcagcagca
     1741 gcctcagtag tcgactagga gtggaccctc gggacccact ggacggcagg cgttccttga
     1801 tctcctggga cccccggagc cccggtgtac ccctgcttcc agacactagc acgttttacg
     1861 gctccctcat tgcagagcag acttccagcc ctccagtccg gccaagcccg cagacgccag
     1921 ctgctaggcg ccttccaccc aagctgaccg gaacctccag cccctgggct agctcagata
     1981 gtctctgcag ccgcagggga ctctgttccc cacgcatgtc tctggcccct gcagaggctt
     2041 ggaaggccaa aaagaagcag gaactgcacc aagctaacag ctccccgctg ctccggggta
     2101 gccaccccat ggaaatctgg gcctgggagt tgggaagcag agcctccaag aacctttctc
     2161 aaagcccggg cccaaacact tgttccccac gagaagctcc cggagcagtg gtagcctggc
     2221 gtgccctggg accacaactt caccgcaact ccagtgagct agcagctcgt ccactccctc
     2281 cgacacccct ttctcttcgt ggagccccca gtcatgatcc gcagagccag tgtgtggaga
     2341 agctccaagc tccctcctct gacccactgc cagcagcccc tctctccgtc ctcaactctt
     2401 ccaggccttc cagcccccag gcctctttcc tctctgttcc tagcccagga tccagcaacc
     2461 tgtccagctc ctcactgtca tccttagagg aggaggatca ggacagtgta ctcacccctg
     2521 aggaggtagc cctgtgtctg gagctcagtg atggggagga gacacccacg aacagtgtat
     2581 ctcctatgcc aagagctcct tcacccccag caacctatgg ctatatcagc ataccaacct
     2641 cctcaggact ggcagacatg ggcagagctg gtgggggcgt ggggtctgag gtcgggaact
     2701 tactgtgccc acctcggctc tgccccacac ctacacccag cgagggctcc ctggccaatg
     2761 gttggggctc agcttcagag gacaatgtcc ccagcgccag ggccagcctg gttagctctt
     2821 ctgatggctc cttccttgct gacgctcact ttgctcgtgc cctggcagtg gctgtggaca
     2881 gcttcggttt cagtctggag cccagggaag ctgactgtgt ctttactgat gcctcatcac
     2941 ccccatcccc tcgggatgat ctttctctga cccgaagctt ctctctgcca ctgtgggagt
     3001 ggaggccaga ctggttggaa gatgctgaga tcaaccacac ccagaggctg gggagggggc
     3061 tgcctccctg gcctcctgat tctagagtct cttcccagcg aagttggctc actggtgttg
     3121 tgcccaaggc tggtgattcc tcctgaattg tccctgagat ggccagaaga ataccaagac
     3181 cactctcctg tctgtcccca ggtcttctcc caggtggagg tcttggctta tgcttctctg
     3241 taatagaggt ccatcatcac tgggcttttg gagagtcctg tcactgggat tgttaaaaca
     3301 aatgaaagca aaccaaatta tgatctaggg agtcttggat tcccactgag aacaaggcag
     3361 catcttcagg acgtcagact ctctcttcac aactagaacc tttggcctaa gtaagcctgg
     3421 cccctatctc ccacccatgt ggatcatgga aagaagggaa gccaaccatg tcttcaggaa
     3481 ggatcgaaat gtctgttggg gagggctata gtggaggacc catggaagag ccctctcata
     3541 cttacttgga ctccttagag accagctcag cccttccccc agccacacca tacaaggaaa
     3601 ctaaaggagc aaagtcatgg atgcagtggg tcctatctag catcacagtc aatgctccaa
     3661 cgtgggatca atggaggagg ctggaggaaa ggaagcggga ggacgtggaa ccaaagtact
     3721 cctcccatgc cactgccact gtgagcttac ggaacttgat tctgtaaaac tgtaaagact
     3781 tgagttctgt gacatcattc cttgggctct atttttggga agggtgcctg cagagatgtt
     3841 aagcgttgcc tggctagcga gggaatcacg tattcactgt gacttagtga atggcctcat
     3901 ttcttcctgc tgtgcaatgg aagggaatga gatatatcct gcacgtttaa aaggaatgta
     3961 aagagggccg agtgggtcta aggtgctata ggtcttggag agccaggtcc tgttttctgc
     4021 agccccagct cggctccctg gagtcaccga ttccagatgg tgtcttaaaa ttagacaggc
     4081 aggcagctaa attccaggga aggtagaatc acctgggata cttggctagt gaacactttt
     4141 tagacacaac tgcaggacag atggaccctg gtggaggaca tgggtcctga accagtgaag
     4201 gccactagag ggcaggctac ctgtatctct ctcacacgtg ctgggtaagg tcatgggcag
     4261 aactgtgctg tagagcacca tcgaggccaa agaagatagc tctggtgtgt gtggagggag
     4321 ggtgtgccgg cagcagggcc caatggtgct ccttccctaa accctgcagc tcttcctgaa
     4381 gaccaccccg agttaggaag tatgacccgg gtggctgatt tctgagctat gctcagtgaa
     4441 ccagcctgac tggcttgcag gatcttgaaa atgggtgtgg cgcacccatt caattctttt
     4501 cctgttttag atttctttct ttctttagct ttctggcaaa attcctcttg aaaccattcc
     4561 aaccctgttt gccagactga aggggaaagg gtttcagcac acaccaatat tcaggtcaaa
     4621 gtggagggcc atgtcatggc tctgaaggtc caagctattt agagaactga ggatgaagca
     4681 agcagccaag gtgtgggtca gctatggggt gagagtgaca gcgaccgtga cgcaagtcta
     4741 tggcagacac cctctgtgaa ggagcctggg gctatgaagc atatacttta ttcttggctg
     4801 gcatgtcact catatactct ttgttttgtt ttgttttgtt ttgtttttaa acttgtattt
     4861 gttgtgatgt gagtgagcag atagaccctg gagaagggca ccaaagaaga ggttggctca
     4921 gttctctgaa gaaggggcaa taatctatgt gttggctata tgaatacccc tatagtaaca
     4981 gtgtgttgtg gtgtagagat aaactatccc tccaacagg
//
