LOCUS       NM_001008299            2035 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), mRNA.
ACCESSION   NM_001008299 XM_214547
VERSION     NM_001008299.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Varshney,D., Petit,A.P., Bueren-Calabuig,J.A., Jansen,C.,
            Fletcher,D.A., Peggie,M., Weidlich,S., Scullion,P., Pisliakov,A.V.
            and Cowling,V.H.
  TITLE     Molecular basis of RNA guanine-7 methyltransferase (RNMT)
            activation by RAM
  JOURNAL   Nucleic Acids Res 44 (21), 10423-10436 (2016)
   PUBMED   27422871
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Gonatopoulos-Pournatzis,T. and Cowling,V.H.
  TITLE     Cap-binding complex (CBC)
  JOURNAL   Biochem J 457 (2), 231-242 (2014)
   PUBMED   24354960
  REMARK    Review article
            Erratum:[Biochem J. 2014 Feb 15;458(1):185]
REFERENCE   3  (bases 1 to 2035)
  AUTHORS   Ghai,R., Bugarcic,A., Liu,H., Norwood,S.J., Skeldal,S.,
            Coulson,E.J., Li,S.S., Teasdale,R.D. and Collins,B.M.
  TITLE     Structural basis for endosomal trafficking of diverse transmembrane
            cargos by PX-FERM proteins
  JOURNAL   Proc Natl Acad Sci U S A 110 (8), E643-E652 (2013)
   PUBMED   23382219
REFERENCE   4  (bases 1 to 2035)
  AUTHORS   Gonatopoulos-Pournatzis,T., Dunn,S., Bounds,R. and Cowling,V.H.
  TITLE     RAM/Fam103a1 is required for mRNA cap methylation
  JOURNAL   Mol Cell 44 (4), 585-596 (2011)
   PUBMED   22099306
REFERENCE   5  (bases 1 to 2035)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   6  (bases 1 to 2035)
  AUTHORS   Bujnicki,J.M., Feder,M., Radlinska,M. and Rychlewski,L.
  TITLE     mRNA:guanine-N7 cap methyltransferases: identification of novel
            members of the family, evolutionary analysis, homology modeling,
            and analysis of sequence-structure-function relationships
  JOURNAL   BMC Bioinformatics 2, 2 (2001)
   PUBMED   11472630
REFERENCE   7  (bases 1 to 2035)
  AUTHORS   Tsukamoto,T., Shibagaki,Y., Niikura,Y. and Mizumoto,K.
  TITLE     Cloning and characterization of three human cDNAs encoding mRNA
            (guanine-7-)-methyltransferase, an mRNA cap methylase
  JOURNAL   Biochem Biophys Res Commun 251 (1), 27-34 (1998)
   PUBMED   9790902
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC085858.1 and CN543861.1.
            
            On Oct 12, 2007 this sequence version replaced NM_001008299.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC085858.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMD00132261, SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1565              BC085858.1         1-1565
            1566-2035           CN543861.1         1-470               c
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..2035
                     /gene="Rnmt"
                     /note="RNA (guanine-7-) methyltransferase"
                     /db_xref="GeneID:291534"
                     /db_xref="RGD:1309242"
     exon            1..52
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            53..460
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1480
                     /gene="Rnmt"
                     /EC_number="2.1.1.56"
                     /note="mRNA cap guanine-N7 methyltransferase; RG7MT1; mRNA
                     cap methyltransferase; mRNA
                     (guanine-N(7)-)-methyltransferase"
                     /codon_start=1
                     /product="mRNA cap guanine-N(7) methyltransferase"
                     /protein_id="NP_001008300.1"
                     /db_xref="GeneID:291534"
                     /db_xref="RGD:1309242"
                     /translation="MESSVKASVDSETESSPGVNETAAASGQRLSEKTRQQADQPKTQ
                     DDLVEQNSSYVQDSPSKKRKLDVEIILDEKHSEDDGGASKRSKLERGGGSEDEPSPGG
                     LTERKRKLQPQDALETQTRKFQKLEEGHSSAVAAHYNELQEVGLVKRSQSRIFYLRNF
                     NNWIKSILIGEILEKVRQRKNRDITVLDLGCGKGGDLLKWRKGRISRLVCADIADISM
                     KQCQQRYEDMKCRRDNEYIFSAEFITADCSKELLVEKFHDPEMYFDICSCQFACHYSF
                     ESLEQADMMLRNACGRLNPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKG
                     NYPLFGCKYDFNLEGVVDVPEFLVYFPLLTEMAKKYNMKLIYKKTFLEFYEEKIKNNE
                     NKMLLKRMQALESYPANENSKLASEKAGDYAHAAEYMKNSQVRLPLGTLSKSEWEATS
                     IYLVFAFEKQQ"
     exon            461..602
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            603..728
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            729..841
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            842..1023
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1188
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1306
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1408
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1442
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..2021
                     /gene="Rnmt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcgtggctc cggccggcgt ttccgcaaac agaatcgtgg atgactgtgt ggtgtcgatt
       61 catgaggttt tgctgtcagt tcaagtaacc agaaatggaa agttctgtga aagcatctgt
      121 agattctgag acagaatctt ctccaggtgt taatgaaacc gctgctgcgt ctgggcagag
      181 gctttctgaa aagacacgcc agcaagcaga tcagccgaaa acacaagatg atcttgtaga
      241 gcaaaattca agttatgtgc aagattctcc atccaagaaa agaaaactag atgtcgaaat
      301 catcctagat gaaaaacact ctgaggatga tggaggtgct tcaaagagaa gcaaactgga
      361 gaggggtggt gggtcagaag atgagccttc ccctggaggg cttaccgaga gaaagcgaaa
      421 actgcagcct caggatgcct tggaaactca gactcggaaa tttcagaagc tggaggaagg
      481 acacagctca gccgtggccg cccactataa cgagcttcag gaagtcggct tggtgaagcg
      541 gagtcaaagt cgcattttct acctaagaaa ctttaataat tggattaaaa gtattcttat
      601 tggggaaatt ttagaaaagg tacgacaaag aaaaaaccgt gacatcactg ttttggacct
      661 gggatgtgga aaaggtggag atttgctcaa gtggaggaag gggagaatta gcaggctagt
      721 gtgcgctgat attgccgata tttctatgaa gcaatgtcaa caacgctacg aagacatgaa
      781 atgtcgtcgt gataatgagt atatttttag tgcagaattt ataactgctg actgttcaaa
      841 ggaacttttg gttgaaaaat tccatgatcc agaaatgtac tttgacatct gcagctgtca
      901 gtttgcttgc cattactcct tcgagtcctt ggagcaggcc gacatgatgc ttagaaacgc
      961 ctgtggaagg cttaaccctg gcggctattt tattggtacc actcccaata gcttcgaact
     1021 gataagacgc cttgaagctt cagaaacaga atcatttgga aatgaaatat acactgtgaa
     1081 attccagaag aaaggaaatt atcctttatt tggctgcaaa tatgacttta acttggaagg
     1141 tgttgtggat gtccctgaat tcttggtcta ttttccattg ctaactgaga tggcaaagaa
     1201 gtataatatg aagctaatct ataaaaaaac atttctggag ttctatgaag aaaagattaa
     1261 gaacaatgaa aacaaaatgc tcttaaaacg gatgcaggcc ctggagtcat accctgcgaa
     1321 cgagaactct aaactggcct ctgaaaaggc gggcgactac gcacatgcag ccgagtacat
     1381 gaagaacagt caagtaaggt tacctctggg aacgttaagt aagtcagaat gggaagctac
     1441 aagcatttac ttggtttttg cctttgagaa gcagcagtga gcgcagtgct tcagagcagc
     1501 cctccatcct cccaggtgga gcagaccatc cagagacatt tgacagccgt ttgtttattt
     1561 tcatagtaag ttctaaatgt gtagggtgct ccacagagtc cagtgtataa atctgacgtt
     1621 tactgtctgt gacaggttag cataatgtgt gtacataagg atgagtcatc gctaagatct
     1681 atttttaagt aatgttgtaa gaattgtttc cctctactgt caaaactatg gcagggcatg
     1741 tcagagcaac tgaccgctcc agtgctgtga tttgctggct gtgtttacag agctgagttt
     1801 cccataactg tagaatgggc agaggaacgc tagattgcca ccgtgcagtc atttcaagcc
     1861 ttgttacagt gtttttcaag atttaatgga ttgtgttcta tacacttgaa tgtaagtgtt
     1921 cgatatatat tgctaaagtt ataaaattaa ttttgattat tgtcataaga gctacctgac
     1981 tagattttga ctgtttcaat aaaaataact atttgctaaa aaaaaaaaaa aaaaa
//
