LOCUS       NM_001025727            1984 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus ring finger protein 8 (Rnf8), mRNA.
ACCESSION   NM_001025727 XM_342108
VERSION     NM_001025727.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Valnegri,P., Huang,J., Yamada,T., Yang,Y., Mejia,L.A., Cho,H.Y.,
            Oldenborg,A. and Bonni,A.
  TITLE     RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the
            mammalian brain
  JOURNAL   Nat Commun 8 (1), 1271 (2017)
   PUBMED   29097665
  REMARK    GeneRIF: Knockdown or conditional knockout of RNF8 or UBC13 in
            rodent cerebellar granule neurons robustly increases the number of
            parallel fiber presynaptic boutons and functional parallel
            fiber/Purkinje cell synapses. In contrast to the role of nuclear
            RNF8 in proliferating cells, RNF8 operates in the cytoplasm in
            neurons to suppress synapse differentiation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Gou,L.T., Kang,J.Y., Dai,P., Wang,X., Li,F., Zhao,S., Zhang,M.,
            Hua,M.M., Lu,Y., Zhu,Y., Li,Z., Chen,H., Wu,L.G., Li,D., Fu,X.D.,
            Li,J., Shi,H.J. and Liu,M.F.
  TITLE     Ubiquitination-Deficient Mutations in Human Piwi Cause Male
            Infertility by Impairing Histone-to-Protamine Exchange during
            Spermiogenesis
  JOURNAL   Cell 169 (6), 1090-1104 (2017)
   PUBMED   28552346
REFERENCE   3  (bases 1 to 1984)
  AUTHORS   Panier,S. and Boulton,S.J.
  TITLE     Double-strand break repair: 53BP1 comes into focus
  JOURNAL   Nat Rev Mol Cell Biol 15 (1), 7-18 (2014)
   PUBMED   24326623
  REMARK    Review article
REFERENCE   4  (bases 1 to 1984)
  AUTHORS   Mattiroli,F., Vissers,J.H., van Dijk,W.J., Ikpa,P., Citterio,E.,
            Vermeulen,W., Marteijn,J.A. and Sixma,T.K.
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage
            signaling
  JOURNAL   Cell 150 (6), 1182-1195 (2012)
   PUBMED   22980979
REFERENCE   5  (bases 1 to 1984)
  AUTHORS   Mallette,F.A., Mattiroli,F., Cui,G., Young,L.C., Hendzel,M.J.,
            Mer,G., Sixma,T.K. and Richard,S.
  TITLE     RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers
            53BP1 recruitment to DNA damage sites
  JOURNAL   EMBO J 31 (8), 1865-1878 (2012)
   PUBMED   22373579
REFERENCE   6  (bases 1 to 1984)
  AUTHORS   Ramachandran,S., Chahwan,R., Nepal,R.M., Frieder,D., Panier,S.,
            Roa,S., Zaheen,A., Durocher,D., Scharff,M.D. and Martin,A.
  TITLE     The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch
            recombination
  JOURNAL   Proc Natl Acad Sci U S A 107 (2), 809-814 (2010)
   PUBMED   20080757
REFERENCE   7  (bases 1 to 1984)
  AUTHORS   Wu,J., Huen,M.S., Lu,L.Y., Ye,L., Dou,Y., Ljungman,M., Chen,J. and
            Yu,X.
  TITLE     Histone ubiquitination associates with BRCA1-dependent DNA damage
            response
  JOURNAL   Mol Cell Biol 29 (3), 849-860 (2009)
   PUBMED   19015238
REFERENCE   8  (bases 1 to 1984)
  AUTHORS   Zhang,S., Chea,J., Meng,X., Zhou,Y., Lee,E.Y. and Lee,M.Y.
  TITLE     PCNA is ubiquitinated by RNF8
  JOURNAL   Cell Cycle 7 (21), 3399-3404 (2008)
   PUBMED   18948756
REFERENCE   9  (bases 1 to 1984)
  AUTHORS   Huen,M.S., Grant,R., Manke,I., Minn,K., Yu,X., Yaffe,M.B. and
            Chen,J.
  TITLE     RNF8 transduces the DNA-damage signal via histone ubiquitylation
            and checkpoint protein assembly
  JOURNAL   Cell 131 (5), 901-914 (2007)
   PUBMED   18001825
REFERENCE   10 (bases 1 to 1984)
  AUTHORS   Mailand,N., Bekker-Jensen,S., Faustrup,H., Melander,F., Bartek,J.,
            Lukas,C. and Lukas,J.
  TITLE     RNF8 ubiquitylates histones at DNA double-strand breaks and
            promotes assembly of repair proteins
  JOURNAL   Cell 131 (5), 887-900 (2007)
   PUBMED   18001824
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC099079.1.
            
            On Jul 22, 2005 this sequence version replaced XM_342108.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099079.1, SRR8487230.81971.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132265, SAMD00132269
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..1984
                     /gene="Rnf8"
                     /note="ring finger protein 8"
                     /db_xref="GeneID:361815"
                     /db_xref="RGD:1308035"
     exon            1..195
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1548
                     /gene="Rnf8"
                     /EC_number="2.3.2.27"
                     /note="ring finger protein 8, E3 ubiquitin protein ligase;
                     RING-type E3 ubiquitin transferase RNF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RNF8"
                     /protein_id="NP_001020898.1"
                     /db_xref="GeneID:361815"
                     /db_xref="RGD:1308035"
                     /translation="MGEPDPLVSGQLAARRSWCLRRLGMDREWLQLEAGSEVTIGRGF
                     SVTYQLISKVCPLMISRNHCVLKQNPEGQWTIMDNKSLNGVWLNRERLAPLQGYCIRK
                     GDHIQLGVPLESKEHAEYEYEVIEEDRESLAPCLAPKNDHTTEKHKGLRTKRKFSSDG
                     VESLPAEGPSDLRCPLAKGSSKPAEPEKLHGKGEAASQPLGCLCPTLASLEASERTAG
                     PHACSTLPKVLELYPKKQKACSPSASQSSLELFKMTMSRMLKLKTQMQEKQIAVLNVK
                     RQARKGSSKKVVRMEKELRDLQSQLYAEQAQQQARVEQLEKTFQEEEQHLQGLEKEQG
                     ECDLKQQLLQALQEHRALMEELDRSKKDFEKIIQAKNKELERTKEEKDKVQAQKEEVL
                     SHMNDVLENELQCIICSEYFIEAVTLNCAHSFCSFCISEWMKRKVECPICRKDIESRT
                     NSLVLDNCISKMVERLSSDVKERRSVLIRERRAKRLL"
     exon            196..324
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            325..1065
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1128
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1218
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1326
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1531
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1953
                     /gene="Rnf8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgcgcgagag gcgcctcgga agcgatcgga gcccaggctg gagtttggct ggttactgaa
       61 agttgccccg gtgccgagcg ggctatgggc gagcccgacc ccttggtctc ggggcagctc
      121 gcggcccgcc ggagctggtg cttgcggcgg ctggggatgg accgcgagtg gctacagctg
      181 gaggctggga gtgaggtgac cataggacgg ggattcagtg tcacgtacca gctgatctca
      241 aaggtgtgcc ctctgatgat ttctcgaaac cactgcgttc tgaagcagaa tcctgaggga
      301 caatggacga ttatggacaa taagagtctg aatggtgttt ggctgaacag agagcgtctg
      361 gcgccgttac agggttactg catccgtaag ggagaccaca tccagctcgg agtgcctctg
      421 gaaagcaagg agcatgcgga gtacgaatac gaagttatcg aagaagaccg ggagagtctg
      481 gctccctgcc ttgcccccaa gaatgaccac acaacggaaa aacataaagg cttgagaact
      541 aagaggaagt tcagttcgga tggggtagag agtcttccag ccgagggccc ctcagatctc
      601 aggtgcccac tggctaaagg ctccagtaaa cccgctgagc cagagaagtt acacggcaaa
      661 ggtgaagcgg caagtcagcc cttgggatgt ttgtgcccta ccttggcgtc tcttgaggcg
      721 agtgagagga ccgcggggcc tcatgcttgc tctactcttc ctaaggtcct cgaactctat
      781 cctaagaagc agaaggcctg cagcccatca gcatctcaga gcagcttaga actgtttaag
      841 atgaccatgt ccaggatgct caagctgaaa acccagatgc aagaaaagca gattgctgtc
      901 ctgaacgtca agaggcaggc ccggaagggg agctcaaaga aagtcgtgag gatggagaag
      961 gagctgcgag acctacagtc ccagctgtac gcagagcagg ctcagcagca ggcaagagtg
     1021 gagcagctgg agaagacgtt ccaggaggag gagcagcacc tccagggttt ggagaaagag
     1081 caaggagagt gtgatctgaa gcaacagctg ctgcaggctc tgcaggagca tcgcgctcta
     1141 atggaagaac tagatcgcag caagaaggac tttgagaaaa tcattcaagc caagaacaaa
     1201 gaattggagc ggaccaagga agaaaaggac aaggtccaag cacagaagga ggaagttctc
     1261 agccacatga acgacgtgct ggagaacgag ctccagtgca tcatctgctc agagtacttc
     1321 atcgaggctg tcaccctgaa ctgtgcccac agtttctgct ccttctgcat cagtgagtgg
     1381 atgaagcgga aagtggagtg tcccatctgt cggaaggaca ttgagtccag aaccaactcg
     1441 ctggttctgg acaactgcat cagtaagatg gtggaacgcc tgagctcaga cgtgaaggag
     1501 aggagaagcg tcctcattag ggaacgaaga gcaaagagac tgttatgaag gtcatgctcc
     1561 aaggacatgt gagagactag gaggatggca gcttggcgcg ttctagagga ttccctttgg
     1621 ctgctctgga catcatcgga agccgtgtgc tgcagagact taggaagtct gtgtcactgg
     1681 ccttctgtct gctggccctg ctgctgacat caccgtctac agtgctgctg ggacacccac
     1741 ttggagtgac tgcctcacta cgtgttaaat gagttgagtt ctgtgtttct tcatttgttg
     1801 attgtttttg atacctcatg caccctgttg acatcgcctt ggacacttgg gtgcgcccta
     1861 tggctgcatg gaaggtggcc tgcattttga gagtctggtt taccttttaa tgaaaacaag
     1921 atgtcatttc ttggaaataa atatataaaa cccaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1981 aaaa
//
