LOCUS       NP_001020898             487 aa            linear   ROD 27-APR-2025
DEFINITION  E3 ubiquitin-protein ligase RNF8 [Rattus norvegicus].
ACCESSION   NP_001020898 XP_342109
VERSION     NP_001020898.1
DBSOURCE    REFSEQ: accession NM_001025727.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 487)
  AUTHORS   Valnegri,P., Huang,J., Yamada,T., Yang,Y., Mejia,L.A., Cho,H.Y.,
            Oldenborg,A. and Bonni,A.
  TITLE     RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the
            mammalian brain
  JOURNAL   Nat Commun 8 (1), 1271 (2017)
   PUBMED   29097665
  REMARK    GeneRIF: Knockdown or conditional knockout of RNF8 or UBC13 in
            rodent cerebellar granule neurons robustly increases the number of
            parallel fiber presynaptic boutons and functional parallel
            fiber/Purkinje cell synapses. In contrast to the role of nuclear
            RNF8 in proliferating cells, RNF8 operates in the cytoplasm in
            neurons to suppress synapse differentiation.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 487)
  AUTHORS   Gou,L.T., Kang,J.Y., Dai,P., Wang,X., Li,F., Zhao,S., Zhang,M.,
            Hua,M.M., Lu,Y., Zhu,Y., Li,Z., Chen,H., Wu,L.G., Li,D., Fu,X.D.,
            Li,J., Shi,H.J. and Liu,M.F.
  TITLE     Ubiquitination-Deficient Mutations in Human Piwi Cause Male
            Infertility by Impairing Histone-to-Protamine Exchange during
            Spermiogenesis
  JOURNAL   Cell 169 (6), 1090-1104 (2017)
   PUBMED   28552346
REFERENCE   3  (residues 1 to 487)
  AUTHORS   Panier,S. and Boulton,S.J.
  TITLE     Double-strand break repair: 53BP1 comes into focus
  JOURNAL   Nat Rev Mol Cell Biol 15 (1), 7-18 (2014)
   PUBMED   24326623
  REMARK    Review article
REFERENCE   4  (residues 1 to 487)
  AUTHORS   Mattiroli,F., Vissers,J.H., van Dijk,W.J., Ikpa,P., Citterio,E.,
            Vermeulen,W., Marteijn,J.A. and Sixma,T.K.
  TITLE     RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage
            signaling
  JOURNAL   Cell 150 (6), 1182-1195 (2012)
   PUBMED   22980979
REFERENCE   5  (residues 1 to 487)
  AUTHORS   Mallette,F.A., Mattiroli,F., Cui,G., Young,L.C., Hendzel,M.J.,
            Mer,G., Sixma,T.K. and Richard,S.
  TITLE     RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers
            53BP1 recruitment to DNA damage sites
  JOURNAL   EMBO J 31 (8), 1865-1878 (2012)
   PUBMED   22373579
REFERENCE   6  (residues 1 to 487)
  AUTHORS   Ramachandran,S., Chahwan,R., Nepal,R.M., Frieder,D., Panier,S.,
            Roa,S., Zaheen,A., Durocher,D., Scharff,M.D. and Martin,A.
  TITLE     The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch
            recombination
  JOURNAL   Proc Natl Acad Sci U S A 107 (2), 809-814 (2010)
   PUBMED   20080757
REFERENCE   7  (residues 1 to 487)
  AUTHORS   Wu,J., Huen,M.S., Lu,L.Y., Ye,L., Dou,Y., Ljungman,M., Chen,J. and
            Yu,X.
  TITLE     Histone ubiquitination associates with BRCA1-dependent DNA damage
            response
  JOURNAL   Mol Cell Biol 29 (3), 849-860 (2009)
   PUBMED   19015238
REFERENCE   8  (residues 1 to 487)
  AUTHORS   Zhang,S., Chea,J., Meng,X., Zhou,Y., Lee,E.Y. and Lee,M.Y.
  TITLE     PCNA is ubiquitinated by RNF8
  JOURNAL   Cell Cycle 7 (21), 3399-3404 (2008)
   PUBMED   18948756
REFERENCE   9  (residues 1 to 487)
  AUTHORS   Huen,M.S., Grant,R., Manke,I., Minn,K., Yu,X., Yaffe,M.B. and
            Chen,J.
  TITLE     RNF8 transduces the DNA-damage signal via histone ubiquitylation
            and checkpoint protein assembly
  JOURNAL   Cell 131 (5), 901-914 (2007)
   PUBMED   18001825
REFERENCE   10 (residues 1 to 487)
  AUTHORS   Mailand,N., Bekker-Jensen,S., Faustrup,H., Melander,F., Bartek,J.,
            Lukas,C. and Lukas,J.
  TITLE     RNF8 ubiquitylates histones at DNA double-strand breaks and
            promotes assembly of repair proteins
  JOURNAL   Cell 131 (5), 887-900 (2007)
   PUBMED   18001824
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC099079.1.
            
            On Jul 22, 2005 this sequence version replaced XP_342109.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099079.1, SRR8487230.81971.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132265, SAMD00132269
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     Protein         1..487
                     /product="E3 ubiquitin-protein ligase RNF8"
                     /EC_number="2.3.2.27"
                     /note="ring finger protein 8, E3 ubiquitin protein ligase;
                     RING-type E3 ubiquitin transferase RNF8"
                     /calculated_mol_wt=55484
     Region          18..126
                     /region_name="FHA_RNF8"
                     /note="forkhead associated (FHA) domain found in RING
                     finger protein 8 (RNF8) and similar proteins; cd22663"
                     /db_xref="CDD:438715"
     Site            order(42,55,57..61,82..83,110)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:438715"
     Region          68..72
                     /region_name="Required for interaction with PIWIL1.
                     /evidence=ECO:0000255|HAMAP-Rule:MF_03067"
                     /note="propagated from UniProtKB/Swiss-Prot (Q4KLN8.1)"
     Region          135..195
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q4KLN8.1)"
     Site            157
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O76064; propagated from
                     UniProtKB/Swiss-Prot (Q4KLN8.1)"
     Region          276..>407
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          402..464
                     /region_name="RING-HC_RNF8"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 8 (RNF8) and similar proteins; cd16535"
                     /db_xref="CDD:438197"
     Site            order(402..403,417..418,421..423,450..455,457..458,
                     461..462)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:438197"
     Site            order(404,406..410,431..432,435..436,440,443)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438197"
     CDS             1..487
                     /gene="Rnf8"
                     /coded_by="NM_001025727.1:85..1548"
                     /db_xref="GeneID:361815"
                     /db_xref="RGD:1308035"
ORIGIN      
        1 mgepdplvsg qlaarrswcl rrlgmdrewl qleagsevti grgfsvtyql iskvcplmis
       61 rnhcvlkqnp egqwtimdnk slngvwlnre rlaplqgyci rkgdhiqlgv pleskehaey
      121 eyevieedre slapclapkn dhttekhkgl rtkrkfssdg veslpaegps dlrcplakgs
      181 skpaepeklh gkgeaasqpl gclcptlasl easertagph acstlpkvle lypkkqkacs
      241 psasqsslel fkmtmsrmlk lktqmqekqi avlnvkrqar kgsskkvvrm ekelrdlqsq
      301 lyaeqaqqqa rveqlektfq eeeqhlqgle keqgecdlkq qllqalqehr almeeldrsk
      361 kdfekiiqak nkelertkee kdkvqaqkee vlshmndvle nelqciicse yfieavtlnc
      421 ahsfcsfcis ewmkrkvecp icrkdiesrt nslvldncis kmverlssdv kerrsvlire
      481 rrakrll
//
