LOCUS       NM_001007015             650 bp    mRNA    linear   ROD 21-MAR-2023
DEFINITION  Rattus norvegicus ribonuclease A family member 2 (Rnase2), mRNA.
ACCESSION   NM_001007015 XM_579169
VERSION     NM_001007015.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Premzl M.
  TITLE     Comparative genomic analysis of eutherian ribonuclease A genes
  JOURNAL   Mol Genet Genomics 289 (2), 161-167 (2014)
   PUBMED   24337645
REFERENCE   2  (bases 1 to 650)
  AUTHORS   Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang
            C, Heman-Ackah SM, Hessa T, Guha R, Martin SE and Youle RJ.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   3  (bases 1 to 650)
  AUTHORS   Pulido D, Moussaoui M, Nogues MV, Torrent M and Boix E.
  TITLE     Towards the rational design of antimicrobial proteins: single point
            mutations can switch on bactericidal and agglutinating activities
            on the RNase A superfamily lineage
  JOURNAL   FEBS J 280 (22), 5841-5852 (2013)
   PUBMED   23992292
REFERENCE   4  (bases 1 to 650)
  AUTHORS   Cho S, Beintema JJ and Zhang J.
  TITLE     The ribonuclease A superfamily of mammals and birds: identifying
            new members and tracing evolutionary histories
  JOURNAL   Genomics 85 (2), 208-220 (2005)
   PUBMED   15676279
REFERENCE   5  (bases 1 to 650)
  AUTHORS   Yang D, Rosenberg HF, Chen Q, Dyer KD, Kurosaka K and Oppenheim JJ.
  TITLE     Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with
            chemotactic activities for dendritic cells
  JOURNAL   Blood 102 (9), 3396-3403 (2003)
   PUBMED   12855582
REFERENCE   6  (bases 1 to 650)
  AUTHORS   Ishihara K, Asai K, Nakajima M, Mue S and Ohuchi K.
  TITLE     Preparation of recombinant rat eosinophil-associated ribonuclease-1
            and -2 and analysis of their biological activities
  JOURNAL   Biochim Biophys Acta 1638 (2), 164-172 (2003)
   PUBMED   12853122
REFERENCE   7  (bases 1 to 650)
  AUTHORS   Nakajima M, Hirakata M, Nittoh T, Ishihara K and Ohuchi K.
  TITLE     Expression and purification of recombinant rat
            eosinophil-associated ribonucleases, homologues of human eosinophil
            cationic protein and eosinophil-derived neurotoxin, and their
            characterization
  JOURNAL   Int Arch Allergy Immunol 125 (3), 241-249 (2001)
   PUBMED   11490157
REFERENCE   8  (bases 1 to 650)
  AUTHORS   Singhania NA, Dyer KD, Zhang J, Deming MS, Bonville CA, Domachowske
            JB and Rosenberg HF.
  TITLE     Rapid evolution of the ribonuclease A superfamily: adaptive
            expansion of independent gene clusters in rats and mice
  JOURNAL   J Mol Evol 49 (6), 721-728 (1999)
   PUBMED   10594173
REFERENCE   9  (bases 1 to 650)
  AUTHORS   Domachowske JB, Bonville CA, Dyer KD and Rosenberg HF.
  TITLE     Evolution of antiviral activity in the ribonuclease A gene
            superfamily: evidence for a specific interaction between
            eosinophil-derived neurotoxin (EDN/RNase 2) and respiratory
            syncytial virus
  JOURNAL   Nucleic Acids Res 26 (23), 5327-5332 (1998)
   PUBMED   9826755
REFERENCE   10 (bases 1 to 650)
  AUTHORS   Nittoh T, Hirakata M, Mue S and Ohuchi K.
  TITLE     Identification of cDNA for rat homologues of human major basic
            protein and eosinophil cationic protein
  JOURNAL   Int Arch Allergy Immunol 117 Suppl 1, 5-9 (1998)
   PUBMED   9758888
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB005291.1.
            
            On Apr 19, 2005 this sequence version replaced XM_579169.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB005291.1, BF543868.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p14"
     gene            1..650
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
                     /note="ribonuclease A family member 2"
                     /db_xref="GeneID:474169"
                     /db_xref="RGD:1359432"
     exon            1..53
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
                     /inference="alignment:Splign:2.1.0"
     exon            54..650
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..526
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
                     /EC_number="4.6.1.18"
                     /note="ribonuclease 5; ribonuclease 14; ribonuclease A f1;
                     ribonuclease 15; pre-eosinophil-associated ribonuclease-2;
                     eosinophil-associated, ribonuclease A family, member 4;
                     ribonuclease, RNase A family, 2 (liver, eosinophil-derived
                     neurotoxin)"
                     /codon_start=1
                     /product="ribonuclease A family member 2 precursor"
                     /protein_id="NP_001007016.1"
                     /db_xref="GeneID:474169"
                     /db_xref="RGD:1359432"
                     /translation="MGVKPLESRLCLLLLLGLVSMLASCQRPTPSQWFAIQHIYNSSY
                     PQCNAAMLRVNSYTGRCKGINTFLHTSFASVVDVCGNPHITCKDGRSTNCHNSSSQVS
                     VTFCNLTTPARIYTQCRYQTTGSVKFYRVACNNRTSQESPMYPVVPVHLDEIF"
     regulatory      632..637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
     polyA_site      650
                     /gene="Rnase2"
                     /gene_synonym="Ear4; R14; R15; R5; Raf1"
                     /note="8 A nucleotides"
ORIGIN      
        1 tgatttccag gacagccagc actcagttcc acaggagcca caacgcacac tgggaaacat
       61 gggtgtgaag ccgcttgagt ccagactttg tctccttctg ctgctgggac ttgtctcaat
      121 gcttgcctca tgccagcgac caaccccttc ccagtggttt gccatccagc acatctataa
      181 tagctcctac ccccaatgta atgctgccat gctgcgtgtc aacagttata caggaagatg
      241 taagggcata aatacttttc ttcacacaag ttttgctagt gttgttgatg tgtgtggcaa
      301 tccacatata acctgtaaag acgggagaag tacaaattgt cataacagtt cctctcaggt
      361 atctgtaact ttctgtaacc tcacaactcc ggcaaggatt tatacccaat gcagatacca
      421 aactacagga tcggtgaagt tctacagagt tgcctgtaac aatagaactt cacaggagag
      481 tcccatgtat ccagtggttc ctgttcacct ggatgagata ttttagttca atgccagcac
      541 tttgtacctt agctcatctg ctactgccaa gatcacagta gtgaagatcc agttcctctg
      601 tctgcactgc ctaaccagac cttttctaac caataaacac ctttgcattc
//
