LOCUS       NM_001398595            7769 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus regulating synaptic membrane exocytosis 1
            (Rims1), transcript variant 1, mRNA.
ACCESSION   NM_001398595 XM_008766936
VERSION     NM_001398595.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Wu,X., Cai,Q., Shen,Z., Chen,X., Zeng,M., Du,S. and Zhang,M.
  TITLE     RIM and RIM-BP Form Presynaptic Active-Zone-like Condensates via
            Phase Separation
  JOURNAL   Mol Cell 73 (5), 971-984 (2019)
   PUBMED   30661983
  REMARK    GeneRIF: RIM1a and RIM-BP2 are plausible organizers of active
            zones, and the formation of RIM and RIM-BP condensates may cluster
            voltage gated calcium channels into nano- or microdomains and
            position the clustered Ca(2+) channels with Ca(2+) sensors on
            docked vesicles for efficient and precise synaptic transmissions.
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Wang,S.S.H., Held,R.G., Wong,M.Y., Liu,C., Karakhanyan,A. and
            Kaeser,P.S.
  TITLE     Fusion Competent Synaptic Vesicles Persist upon Active Zone
            Disruption and Loss of Vesicle Docking
  JOURNAL   Neuron 91 (4), 777-791 (2016)
   PUBMED   27537483
REFERENCE   3  (bases 1 to 7769)
  AUTHORS   Tang,A.H., Chen,H., Li,T.P., Metzbower,S.R., MacGillavry,H.D. and
            Blanpied,T.A.
  TITLE     A trans-synaptic nanocolumn aligns neurotransmitter release to
            receptors
  JOURNAL   Nature 536 (7615), 210-214 (2016)
   PUBMED   27462810
REFERENCE   4  (bases 1 to 7769)
  AUTHORS   Shen,Y., Ge,W.P., Li,Y., Hirano,A., Lee,H.Y., Rohlmann,A.,
            Missler,M., Tsien,R.W., Jan,L.Y., Fu,Y.H. and Ptacek,L.J.
  TITLE     Protein mutated in paroxysmal dyskinesia interacts with the active
            zone protein RIM and suppresses synaptic vesicle exocytosis
  JOURNAL   Proc Natl Acad Sci U S A 112 (10), 2935-2941 (2015)
   PUBMED   25730884
REFERENCE   5  (bases 1 to 7769)
  AUTHORS   Girach,F., Craig,T.J., Rocca,D.L. and Henley,J.M.
  TITLE     RIM1alpha SUMOylation is required for fast synaptic vesicle
            exocytosis
  JOURNAL   Cell Rep 5 (5), 1294-1301 (2013)
   PUBMED   24290762
  REMARK    GeneRIF: RIM1alpha SUMOylation at K502 is required for its binding
            to Cav2.1 and for fast synaptic vesicle exocytosis.
REFERENCE   6  (bases 1 to 7769)
  AUTHORS   Schoch,S., Castillo,P.E., Jo,T., Mukherjee,K., Geppert,M., Wang,Y.,
            Schmitz,F., Malenka,R.C. and Sudhof,T.C.
  TITLE     RIM1alpha forms a protein scaffold for regulating neurotransmitter
            release at the active zone
  JOURNAL   Nature 415 (6869), 321-326 (2002)
   PUBMED   11797009
REFERENCE   7  (bases 1 to 7769)
  AUTHORS   Coppola,T., Magnin-Luthi,S., Perret-Menoud,V., Gattesco,S.,
            Schiavo,G. and Regazzi,R.
  TITLE     Direct interaction of the Rab3 effector RIM with Ca2+ channels,
            SNAP-25, and synaptotagmin
  JOURNAL   J Biol Chem 276 (35), 32756-32762 (2001)
   PUBMED   11438518
REFERENCE   8  (bases 1 to 7769)
  AUTHORS   Betz,A., Thakur,P., Junge,H.J., Ashery,U., Rhee,J.S., Scheuss,V.,
            Rosenmund,C., Rettig,J. and Brose,N.
  TITLE     Functional interaction of the active zone proteins Munc13-1 and
            RIM1 in synaptic vesicle priming
  JOURNAL   Neuron 30 (1), 183-196 (2001)
   PUBMED   11343654
REFERENCE   9  (bases 1 to 7769)
  AUTHORS   Wang,Y., Sugita,S. and Sudhof,T.C.
  TITLE     The RIM/NIM family of neuronal C2 domain proteins. Interactions
            with Rab3 and a new class of Src homology 3 domain proteins
  JOURNAL   J Biol Chem 275 (26), 20033-20044 (2000)
   PUBMED   10748113
REFERENCE   10 (bases 1 to 7769)
  AUTHORS   Wang,Y., Okamoto,M., Schmitz,F., Hofmann,K. and Sudhof,T.C.
  TITLE     Rim is a putative Rab3 effector in regulating synaptic-vesicle
            fusion
  JOURNAL   Nature 388 (6642), 593-598 (1997)
   PUBMED   9252191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000213.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001398595.2.
            
            On Dec 20, 2021 this sequence version replaced XM_008766936.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF199333.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5756307,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-614               JACYVU010000213.1  14546328-14546941   c
            615-695             JACYVU010000213.1  14465259-14465339   c
            696-764             JACYVU010000213.1  14372094-14372162   c
            765-978             JACYVU010000213.1  14338499-14338712   c
            979-990             JACYVU010000213.1  14336100-14336111   c
            991-1328            JACYVU010000213.1  14270842-14271179   c
            1329-2170           JACYVU010000213.1  14266474-14267315   c
            2171-2238           JACYVU010000213.1  14216872-14216939   c
            2239-2349           JACYVU010000213.1  14214955-14215065   c
            2350-2449           JACYVU010000213.1  14213261-14213360   c
            2450-2573           JACYVU010000213.1  14209633-14209756   c
            2574-2620           JACYVU010000213.1  14205915-14205961   c
            2621-2733           JACYVU010000213.1  14202438-14202550   c
            2734-2864           JACYVU010000213.1  14197310-14197440   c
            2865-3036           JACYVU010000213.1  14195414-14195585   c
            3037-3190           JACYVU010000213.1  14195155-14195308   c
            3191-3262           JACYVU010000213.1  14194090-14194161   c
            3263-3419           JACYVU010000213.1  14189626-14189782   c
            3420-3545           JACYVU010000213.1  14188798-14188923   c
            3546-3608           JACYVU010000213.1  14187230-14187292   c
            3609-3698           JACYVU010000213.1  14182559-14182648   c
            3699-3770           JACYVU010000213.1  14165762-14165833   c
            3771-3953           JACYVU010000213.1  14160209-14160391   c
            3954-4066           JACYVU010000213.1  14159009-14159121   c
            4067-4179           JACYVU010000213.1  14141860-14141972   c
            4180-4346           JACYVU010000213.1  14136440-14136606   c
            4347-4582           JACYVU010000213.1  14110113-14110348   c
            4583-4721           JACYVU010000213.1  14058573-14058711   c
            4722-4834           JACYVU010000213.1  14055949-14056061   c
            4835-4936           JACYVU010000213.1  14052636-14052737   c
            4937-5076           JACYVU010000213.1  14051683-14051822   c
            5077-7769           JACYVU010000213.1  14047498-14050190   c
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q13"
     gene            1..7769
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /note="regulating synaptic membrane exocytosis 1"
                     /db_xref="GeneID:84556"
                     /db_xref="RGD:620000"
     exon            1..614
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             451..5295
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /note="isoform 1 is encoded by transcript variant 1;
                     regulating synaptic membrane exocytosis protein 1; Rab3
                     effector; RIM 1; rab3-interacting molecule 1;
                     rab-3-interacting molecule 1; Rim1b protein"
                     /codon_start=1
                     /product="regulating synaptic membrane exocytosis protein
                     1 isoform 1"
                     /protein_id="NP_001385524.1"
                     /db_xref="GeneID:84556"
                     /db_xref="RGD:620000"
                     /translation="MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQK
                     EEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPE
                     RDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRT
                     KFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSD
                     TATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALG
                     PEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKE
                     RRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPP
                     EEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRA
                     RAPAAQPPTEHGPPPPRPAPGPAEPPEPRVPEPLRKQGRLDPGSAVLLRKAKREKAES
                     MLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELE
                     SESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPK
                     ESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGAT
                     NEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISV
                     ISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKM
                     YFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEE
                     SEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQR
                     SQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHR
                     GDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEP
                     DSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKS
                     ERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVR
                     TLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIE
                     QASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSS
                     DSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKS
                     TSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTE
                     SGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDS
                     QFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKST
                     PAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRM
                     DHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPP
                     CIRS"
     exon            615..695
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            696..764
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            765..978
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            979..990
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1328
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..2170
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2238
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2349
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2449
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2573
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2620
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2733
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2864
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..3036
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3190
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3262
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3419
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3545
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3546..3608
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..3698
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3699..3770
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3771..3953
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4066
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4067..4179
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4180..4346
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4347..4582
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4583..4721
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4722..4834
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4835..4936
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            4937..5076
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
     exon            5077..7769
                     /gene="Rims1"
                     /gene_synonym="Rim1; RIM1a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaactggagg cagcggagcc cgcgagcgag cagcgtcggg cggccccggc ccggccccgc
       61 ccccggcccg ccagcccagc ccgcgcccgg ccgcgctccg gagctcagcg ccagccctcg
      121 ccccctcgcc gtgcctccgc ctgcccgccc ccgccggcca aggctgggct gcgggaggcg
      181 gccgggcggc cccgagcctc gccagggcga cccaaacaaa ggcagcatcc gggctcggcg
      241 ggaggcagag catcatgaga gactgggtcc tcgctgtccc cggctccgct gctgctgccg
      301 ctgccgccgc cgccgccgcc gccgctgccg ctgccgccgc caggagggct tcgctgtgag
      361 cgttggaaag caggggcgca gctgcggggc gtgaatccga aaggtgagag ccagagccag
      421 cgagccgagg gggcgggcag gccacgaaaa atgtcctcgg ccgtggggcc ccgaggtcct
      481 cgcccaccca cggtgcctcc ccctatgcaa gaactgcccg acctgagcca cctgaccgag
      541 gaggagagga acattatcat ggcagtgatg gaccggcaga aggaagagga ggaaaaagaa
      601 gaggccatgc tcaagtgtgt tgtcagggac atggcgaagc ctgctgcctg caaaacacca
      661 agaaatgctg aaagccagcc ccatcaacca ccactgaaca ttttcagatg tgtctgtgtt
      721 cccagaaagc caagcagcga agagggaggc ccagaaagag actggagatt gcatcaacag
      781 tttgaaagct acaaggagca agtgagaaaa atcggagagg aagcgaggcg ttaccagggc
      841 gagcacaagg atgatgcccc gacgtgtgga atctgtcata agacaaagtt tgctgatgga
      901 tgtggccatc tctgctccta ttgtcgcacc aagttctgtg cacgctgcgg aggccgtgtg
      961 tctctgcgat cgaacaatga ggacaaagtg gttatgtggg tatgcaattt atgtcgaaag
     1021 caacaagaaa tcttaacgaa atctggagcg tggttctttg gaagtggccc tcagcagcct
     1081 agtcaagatg ggactctgag tgacacggcc acaggtgctg gatctgaggt gccaagagaa
     1141 aagaaagcaa ggctccaaga gcgatcaagg tctcagacgc ccttgagtac agcagctgtc
     1201 tcttcccaag acactgctac ccccggtgca ccgttggaca ggaacaaagg ggctgagccc
     1261 tcacagcaag ccttgggtcc tgaacagaag caggcatcaa gatcaagaag cgagccaccg
     1321 agggaaagga agaaggctcc agggctttca gagcagaatg gcaagggagg ccagaagagc
     1381 gagcgcaaac gtgtccccaa gtctgtggtg caacccgggg aagggatcgc ggatgagagg
     1441 gagaggaaag agaggcggga aacccgcagg ttggagaaag ggcgctccca ggactactca
     1501 gaccggcctg agaaacgcga caatggcagg gtggcggaag accagaagca gaggaaggag
     1561 gaggagtacc agactaggta ccgcagcgac cctaacctgg ctcgctaccc ggtgaaggcg
     1621 ccgccagagg agcagcagat gcgcatgcac gcccgggtgt cccgagcgag gcacgagcgg
     1681 cgccacagcg acgtggcgct cccgcacacc gaggcagctg ccgccgcgcc ggctgaggcc
     1741 acggcgggca agcgcgcgcc ggccaccgcc agggtctctc ccccggagtc cccgcgcgca
     1801 cgcgcgcccg ccgcccagcc tcccaccgag cacgggccac cgccgccgcg gccagccccg
     1861 ggtcccgcag agccacccga gccgcgcgtc cccgagccgc tccgtaagca gggccgcctg
     1921 gacccgggct cggccgtgct tctgcgcaag gccaagcgcg agaaggcgga gagcatgctg
     1981 cggaacgact cgctgagctc cgatcagtcc gagtccgtgc ggccatcccc gcccaagcct
     2041 caccggccca agcggggagg caagagacgt cagatgtcgg tgagcagctc ggaggaggag
     2101 ggcgtgtcca caccggagta cacgagctgc gaggacgtgg agctggagag cgagagcgtg
     2161 agcgagaaag gtgacttgga ttactactgg ttggatcccg ccacgtggca cagcagggaa
     2221 acgtcgccta tcagttcgca tcctgtaacg tggcagccgt ctaaagaggg agatcgacta
     2281 atcggccgtg ttattcttaa caaaagaaca accatgccca aagaatcagg tgcattattg
     2341 ggtctgaagg tggttggagg aaaaatgacg gacttagggc gccttggtgc tttcatcacc
     2401 aaagtaaaga agggcagcct ggcagacgtc gtcggacacc taagagcagg ggacgaagtc
     2461 ctagagtgga atggtaaacc cctgccggga gcaacaaacg aagaagttta caacattatc
     2521 ttagaatcaa aatcagaacc tcaagttgag attattgttt caaggcctat tggtgacatc
     2581 cccaggatcc ctgagagttc ccatcctccc ctggagtcca gttcaagttc ctttgaatct
     2641 cagaaaatgg aaaggccttc tatttctgtt atttctccaa ccagccctgg agctctgaaa
     2701 gatgccccac aagtcttacc agggcaactc tcagtgaagc tatggtatga taaagtgggg
     2761 caccagctga ttgtaaatgt tctacaagca acagatctac cccctagagt agatggccgt
     2821 cccaggaatc cctatgtaaa aatgtatttt cttccagata gaagcgacaa aagtaaaagg
     2881 agaaccaaaa cagtaaagaa acttctagag ccaaaatgga accagacatt tgtctactca
     2941 cacgtacatc gtagagattt tcgagagcga atgttagaga ttaccgtgtg ggaccagccg
     3001 agagtacagg acgaagagag tgaatttctt ggagagatcc tcatagagtt ggaaacagcg
     3061 cttttagatg atgagcccca ttggtataaa ctccagacac atgacgaatc ttcactacct
     3121 ctgcctcagc catcaccgtt catgcccagg cggcatattc atggagagag ctccagcaaa
     3181 aagctacaaa gatctcagcg aatcagtgat agtgacatct cagattatga ggttgatgat
     3241 ggtattggag tagtgcctcc agtgggttat agagctagtg ctagagagag taaagcaacc
     3301 acgttaacag tgccagagca acaaagaact acacatcacc gctcacgttc cgtgtctcct
     3361 catcgcggcg atgatcaggg aaggcctcgt tcacgtttac caaatgtgcc attacagagg
     3421 agcttagatg aaattcatcc aacacgaagg tcacgttctc caacccgaca ccatgatgcc
     3481 tcccgaagcc cggccgatca cagatccaga catgtggaaa gtcaatattc gtcagagcca
     3541 gacagtgagc ttctcatgct gcccagagca aaacgaggac gaagtgcaga aagcctacac
     3601 atgaccagtg aactgcagcc ctctcttgac agggctagga gtgctagtac caactgcttg
     3661 agaccagata ctagtttgca ttcaccagaa cgagaaaggc actccagaaa gtctgaaaga
     3721 tgtagcatcc aaaaacagtc taggaaaggc acagcctctg atgcagacag ggttctccca
     3781 ccatgccttt ctagaagggg atacgcaacc ccaagagcaa ccgatcaacc ggtcgttagg
     3841 ggaaagcatc ccactcgttc acggtcgagc gagcactcta gtgtcagaac cctgtgttct
     3901 atgcaccacc ttgcccccgg agggtcggcg ccaccttctc cacttctgac aagaacgcac
     3961 cgacaaggaa gcccaaccca gtctcctcca gcagacacat ccttcggcag tcgccgtgga
     4021 agacagctcc cacaggtgcc agttcgaagc ggcagtatag aacaagcaag cttagtagtg
     4081 gaggagcgaa cgagacagat gaaaatgaaa gttcaccgat ttaagcagac aacagggtct
     4141 gggtctagtc aagaacttga ccacgagcaa tactccaagt acaacataca taaagatcag
     4201 tacagaagct gtgataacgc gtctgccaag tcttcagata gtgatgtcag tgatgtgtcc
     4261 gccatttcca gagccagcag tacctcacgc ctcagcagca caagctttat gtcagagcag
     4321 tctgagcgcc ccaggggtag gatcagttca tttaccccca aaatgcaagg cagacggatg
     4381 gggacttcag gaagagccat catcaagagc accagtgtaa gtggagagat atatacactg
     4441 gaacgtaatg acggtagcca gtcggacacg gccgtgggta ccgtcggagc cggtggaaag
     4501 aaacgaagat ccagcctgag cgccaaagtg gtagccattg tgtctcgaag aagcaggagc
     4561 acgtcacagc tcagccagac agagtcgggc cacaagaagt tgaaaagcac catccagagg
     4621 agtacggaaa caggaatggc agctgaaatg cggaagatgg tgagacagcc gagccgggag
     4681 tccacggatg gcagcatcaa cagttatagc tcggaaggaa acttgatatt tcctggagtt
     4741 cgagtaggac ccgacagtca gttcagtgat ttccttgatg ggttgggacc agcgcagctc
     4801 gttggccgtc agacgctcgc caccccggcc atgggcgata tccaaatcgg gatggaggat
     4861 aagaagggtc agttggaggt tgaggttatc agagcccgga gccttacaca aaaacctggt
     4921 tccaaatcta cacccgctcc ctatgtgaaa gtatatcttt tggaaaatgg agcctgtatt
     4981 gccaaaaaga agacaagaat tgcacggaaa actctcgatc ctttgtatca gcagtccctg
     5041 gtttttgatg aaagtccaca gggtaaagtt cttcaggtga ttgtctgggg tgactatgga
     5101 agaatggacc acaaatgctt tatgggtgtg gctcaaatct tgttggaaga acttgatcta
     5161 tccagcatgg tgattggatg gtataaattg ttccctccgt cctcactggt ggatcccact
     5221 ctcgctcccc tgacccgccg ggcttcccaa tcatctctgg aaagttcgtc cgggcctccc
     5281 tgcatccggt catagtaaac tcctatcctt ccagccaaac tctacatttc aggataatta
     5341 tcagaaccag attatttcat gatcaagagc attgttggag acagacaatc aacttgtgtt
     5401 ttgcctgtag tagtttttcg ataatatgtc ccaattgttg tttaaaatat ggcttcatat
     5461 gacagaacaa agcagtcagt caaattgcaa taagaacgga catgctagtg agagccactt
     5521 tacacttctt gcaaatagga aaaacaaaag aggaaacctc aaattcacac acacacacac
     5581 acacatacac accaaattga acaaactgga aattctcact ctgtgaaaag gtgtacttca
     5641 catgtttctg cacagaccac aagcagtgtt gtttccctag tgtttgagat tcttcagttc
     5701 tgtgtgcttt cgttgtttgt gtgctgtttg ctgattcttt ttgccccgtt gcctctcttt
     5761 ctctgtcttc attgtattta ttatttccgc tagctttttt ttttttcatt tttttttgtc
     5821 tttgtctttc tttgctttgc catgacagga ttaggtggtg ttcacagaga cccttccttt
     5881 ctttttccaa aagcaccaaa aagggctgaa gcagtctctg tagcattttc cccgaagttt
     5941 tcaaaagctg ggcgaggcga agccagggaa gggtatcaca ggcagtcact ttacagttct
     6001 tccaagtctt gtatgtggaa ttcccacccc tactcccccc cccaaaaaaa gaatcaccac
     6061 ccaaactagt agtcacagaa tgacttttca gtgaagagca tatctgtttg catcccagat
     6121 aatttgtttc tgttttccat tgtttgtcat ggaagacttt gaagtcgatc tagagccaat
     6181 gctggggatg taggctaacc acattacagt gcttcagcaa cctcttttgc aatgctgata
     6241 gcaaaaggaa aaagtgctca tgtggggaaa ccatgctctc aacctcccaa aatggtgact
     6301 aagaatggat ttgagcctaa gacactttca gctatgccca ggctgctcag ccatggccca
     6361 gagtagaccc atctgctctg ggtcgagcat ggtatgagct gtttaatatt gcactggaaa
     6421 ttccagttca tctactctgc actaaattaa tgtttttatt ggatttcagt ttacatttct
     6481 ttaaggtcaa tgcaagcaca aagtttgatt tttttttaaa tgcacaatat cttaccttgt
     6541 gggggagggt ggggagaaaa agaatacaac caaatgccaa cttgcatatt cttcatttgg
     6601 gttttcttgg ccttggattt ccctcgtgaa ccctccgtgt atgtgctgca ggcacccggt
     6661 cctacagctg catcgatatg ccccctcccc cttcctttga tgagtcgttc aacttttgtg
     6721 atttcacagt tagagcagca tgacttggag ctgttcaaag ctgcatgcac attttgtata
     6781 aaaaatatat gaaaaagaac gaaaaaacaa gcaaagatac aaaaggttat ttaataatgt
     6841 atgttagctc cacataggcc ggttgtatgt tgcatgtagt tgtacagtct gcttgttaat
     6901 tactataccg aaataaccaa gaaatctaag ccatccattt ttgttataga cattttgcag
     6961 gttttagcca gactcggtat gtgggtctgg ggtgaaatgt ctatagatgg actttatttt
     7021 ttaccctctg gaaaaaaaaa aaaaaaaacg tgatgtagtc tggaccaaaa ttccttctaa
     7081 taaatatttt ggtgtagatt cctcctgtgg ggctgtaaca catgtagaca ctgtgtaaac
     7141 actaggtttt aatccataga catactgcct gcaccaagtg aatcatttct tattatttac
     7201 acatgtcaga attacctgcc cattactcca cgggacacag attgaccacc acccaccatg
     7261 ctgtgcagga tgacctgagg caatcggtgc acttctacta gattccgttc tgtcttagtg
     7321 gccaacaacc aaaccaacta taattgggta gttatctttc tattttgacc acttgtctta
     7381 acaaaccccc tcccccccgt gcttttcaat gaactcccca ctcatgtatg taaacatgtt
     7441 taataaaatt tccttttcat gtcagttggc agccatccgt gttctatgtt ttgccagttt
     7501 tccactgcgg gtcctccgga aagggaaagg gaggcttggg tttgctgtct gctgtgggca
     7561 tttggctggc ccgtcactgc actctctgtg tcttcaggga cgacctggaa ggcactttgc
     7621 attccctctg tcagcctgtg taagtgagca catgactgta cgtctgaaaa gcggtatcat
     7681 taaaaacaat ctcagtgaga accaagtgac ggttgtttct ctgctgctgt ttgaatgcta
     7741 ataaaaggag acaatcctgt tgtccactt
//
