LOCUS       NM_001100520            2567 bp    mRNA    linear   ROD 01-DEC-2024
DEFINITION  Rattus norvegicus RIC8 guanine nucleotide exchange factor A
            (Ric8a), mRNA.
ACCESSION   NM_001100520 XM_001058411 XM_215118
VERSION     NM_001100520.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Seven,A.B., Hilger,D., Papasergi-Scott,M.M., Zhang,L., Qu,Q.,
            Kobilka,B.K., Tall,G.G. and Skiniotis,G.
  TITLE     Structures of Galpha Proteins in Complex with Their Chaperone
            Reveal Quality Control Mechanisms
  JOURNAL   Cell Rep 30 (11), 3699-3709 (2020)
   PUBMED   32126208
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   McClelland,L.J., Zhang,K., Mou,T.C., Johnston,J., Yates-Hansen,C.,
            Li,S., Thomas,C.J., Doukov,T.I., Triest,S., Wohlkonig,A.,
            Tall,G.G., Steyaert,J., Chiu,W. and Sprang,S.R.
  TITLE     Structure of the G protein chaperone and guanine nucleotide
            exchange factor Ric-8A bound to Galphai1
  JOURNAL   Nat Commun 11 (1), 1077 (2020)
   PUBMED   32103024
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2567)
  AUTHORS   Zeng,B., Mou,T.C., Doukov,T.I., Steiner,A., Yu,W.,
            Papasergi-Scott,M., Tall,G.G., Hagn,F. and Sprang,S.R.
  TITLE     Structure, Function, and Dynamics of the Galpha Binding Domain of
            Ric-8A
  JOURNAL   Structure 27 (7), 1137-1147 (2019)
   PUBMED   31155309
  REMARK    GeneRIF: Structure, function, and dynamics of the Galpha binding
            domain of Ric-8A have been reported.
REFERENCE   4  (bases 1 to 2567)
  AUTHORS   Kant,R., Zeng,B., Thomas,C.J., Bothner,B. and Sprang,S.R.
  TITLE     Ric-8A, a G protein chaperone with nucleotide exchange activity
            induces long-range secondary structure changes in Galpha
  JOURNAL   Elife 5, e19238 (2016)
   PUBMED   28008853
  REMARK    GeneRIF: In contrast to G protein-coupled receptors, Ric-8A
            interacts with Switches I and II of Galpha and possibly at the
            Galpha domain interface.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2567)
  AUTHORS   Black,L.A., Thomas,C.J., Nix,G.N., Terwilliger,M.C., Sprang,S.R.
            and Ross,J.B.A.
  TITLE     Nanosecond Dynamics of Galphai1 Bound to Nucleotides or Ric-8A, a
            Galpha Chaperone with GEF Activity
  JOURNAL   Biophys J 111 (4), 722-731 (2016)
   PUBMED   27558716
  REMARK    GeneRIF: Induction of Galphai1 structural heterogeneity by Ric-8A
            provides a mechanism for nucleotide release
REFERENCE   6  (bases 1 to 2567)
  AUTHORS   Thomas,C.J., Tall,G.G., Adhikari,A. and Sprang,S.R.
  TITLE     Ric-8A catalyzes guanine nucleotide exchange on G alphai1 bound to
            the GPR/GoLoco exchange inhibitor AGS3
  JOURNAL   J Biol Chem 283 (34), 23150-23160 (2008)
   PUBMED   18541531
  REMARK    GeneRIF: Ric-8A has a role in catalyzing guanine nucleotide
            exchange on G alphai1 bound to the GPR/GoLoco exchange inhibitor
            AGS3
REFERENCE   7  (bases 1 to 2567)
  AUTHORS   Wang,S.C., Lai,H.L., Chiu,Y.T., Ou,R., Huang,C.L. and Chern,Y.
  TITLE     Regulation of type V adenylate cyclase by Ric8a, a guanine
            nucleotide exchange factor
  JOURNAL   Biochem J 406 (3), 383-388 (2007)
   PUBMED   17593019
REFERENCE   8  (bases 1 to 2567)
  AUTHORS   Tonissoo,T., Koks,S., Meier,R., Raud,S., Plaas,M., Vasar,E. and
            Karis,A.
  TITLE     Heterozygous mice with Ric-8 mutation exhibit impaired spatial
            memory and decreased anxiety
  JOURNAL   Behav Brain Res 167 (1), 42-48 (2006)
   PUBMED   16221497
REFERENCE   9  (bases 1 to 2567)
  AUTHORS   Tall,G.G. and Gilman,A.G.
  TITLE     Resistance to inhibitors of cholinesterase 8A catalyzes release of
            Galphai-GTP and nuclear mitotic apparatus protein (NuMA) from
            NuMA/LGN/Galphai-GDP complexes
  JOURNAL   Proc Natl Acad Sci U S A 102 (46), 16584-16589 (2005)
   PUBMED   16275912
REFERENCE   10 (bases 1 to 2567)
  AUTHORS   Tall,G.G., Krumins,A.M. and Gilman,A.G.
  TITLE     Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein
            guanine nucleotide exchange factor
  JOURNAL   J Biol Chem 278 (10), 8356-8362 (2003)
   PUBMED   12509430
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC160852.1.
            
            On or before Sep 7, 2008 this sequence version replaced
            XM_001058411.1, XM_215118.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC160852.1, AY177754.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..2567
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /note="RIC8 guanine nucleotide exchange factor A"
                     /db_xref="GeneID:293614"
                     /db_xref="RGD:727957"
     exon            1..315
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     CDS             232..1824
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /note="synembryn-A; resistance to inhibitors of
                     cholinesterase 8A; heterotrimeric G protein guanine
                     nucleotide exchange factor Ric-8A; resistance to
                     inhibitors of cholinesterase 8A-like protein; resistance
                     to inhibitors of cholinesterase 8 homolog"
                     /codon_start=1
                     /product="chaperone Ric-8A"
                     /protein_id="NP_001093990.1"
                     /db_xref="GeneID:293614"
                     /db_xref="RGD:727957"
                     /translation="MEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRK
                     RLAKLLVSVLEQGLSPKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADIAISE
                     EPIPQPPDMDVLLESLKCLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEV
                     QFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPLVILPAQETE
                     RAMEILKVLFNITYDSVKREVDEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVN
                     LLGNLPLKCLDVLLALELHEGSLEFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVL
                     SVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAA
                     EFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTEEYREAKA
                     SINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSRHRLIQPMGMSPRGHLTS
                     LQDAMCETMEGQLSSDPDSDPD"
     exon            316..363
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            364..954
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1046
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1197
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1293
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1438
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1583
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1703
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..2542
                     /gene="Ric8a"
                     /gene_synonym="Ric-8A; Ric8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgcttagtt agctagcatc gctgctccaa gacaagccgc tcagcggcca cagccgggtt
       61 gctccacctt aagccggagg ccagccccgc gcctgtacac accctaggct cggctgttct
      121 cccagcattc ctcacgcgtg gacgctgggc ttccggacct gggcggagtc cccgggtttg
      181 gccgattgcg tcttcccagc ccaagtcttc tagcaccccg tgccaggggc catggagccc
      241 cgggcagttg cggatgcctt ggagacggga gaggaagatg cggtgacaga agctctgcgg
      301 tcgtttaacc gagagcattc ccagagcttc accttcgatg atgcacagca ggaggacagg
      361 aagagactcg caaagctgct ggtctccgta ctggagcagg gcttgtcacc aaaacaccgt
      421 gtcacctggc tgcagactat ccgaatccta tcccgagacc gcagctgcct ggactcattt
      481 gctagccgcc agagtttaca cgcactagcc tgctatgccg acattgccat ctcagaggag
      541 cccatcccac agcccccaga catggatgtc ctcctcgagt ctctcaaatg cctgtgtaat
      601 cttgtgctca gcagtcctac agcacagatg ctagcagcag aggctcgact ggtggtgagg
      661 ctagcggaac gtgtgggact gtaccgcaaa aggagctatc cccacgaagt ccagttcttt
      721 gacttaaggc tccttttcct gctaacagcc cttcgcactg atgtgcgcca gcagctgttt
      781 caggagctgc atggcgtgcg cctgctgacc gatgcgctgg aactgacatt gggtgtggcc
      841 cccaaagaaa accctctggt gatacttcca gcccaagaga cagagcgggc catggaaatc
      901 ctcaaagtgc tctttaacat cacctacgac tctgttaaga gggaggttga tgaggaggac
      961 gctgcccttt accggtacct ggggaccctt ctgcggcact gtgtgatggc tgatgctgct
     1021 ggagaccgca cagaggagtt ccatggccac accgtgaatc tcctggggaa cctgcccctc
     1081 aaatgtttgg atgtccttct cgctctggag ctacacgaag gatccttaga gtttatggga
     1141 gttaatatgg atgtgatcaa tgccctcctt gccttcctag agaagcgtct gcaccagacc
     1201 cacaggctga aggagtgtgt ggcaccggtg ctgagcgtgt tgacagaatg tgcccgcatg
     1261 caccgtcctg ccaggaagtt cctgaaggcc caggtcctgc cccctctgag ggatgtgagg
     1321 actcggcctg aggtggggga cctgctccga aacaagcttg tccgcctcat gacacacctg
     1381 gatacagatg tgaagagagt agctgctgag ttcctctttg tcttatgctc tgagagtgtg
     1441 ccccgattca tcaagtacac aggctacggg aatgctgctg gcctcctggc tgccaggggc
     1501 ctcatggctg ggggccggcc cgagggccag tactcagagg atgaggacac cgacacagag
     1561 gagtacaggg aagctaaggc cagcatcaac cctgtgactg gaagggtaga ggagaagccg
     1621 cctaatccta tggaaggcat gacagaggag cagaaggaac atgaggccat gaagctagtg
     1681 aacatgtttg acaagctctc caggcacaga ctcatccaac ccatggggat gagtcccagg
     1741 ggtcacctca cttctctgca agatgcgatg tgtgaaacta tggagggaca gctctcctca
     1801 gaccccgact cagaccccga ctgaagatgg cagccacttt gctccccttc agaactggtg
     1861 ctgtttccag acatgcctgg ggtgctgcct cgagaagcca cacccctctc cagctgggga
     1921 gccgttctct ctactcctca catatctgtc atctgccttt ggtccagttt cttgtgttta
     1981 ggactgtggt agtcttatcc tctgtgaaga cagggaaccc acctttcatt tccacaagtg
     2041 aagttgtttc ttggatgtgg ctgcagccat gggcagagtt gccttggcac ataaatgagc
     2101 gtgcatgtgt gagggcggta gctgagcagg gtgcacacgg tgtaagcatg gctgggctgg
     2161 taccagccca gagcccgtat gtggggcata tgctgtgttt caaacattct agaatctgtt
     2221 gaaattgttt aagtatgtca gaaaacacac caagcatcat ggtttctgct tctggcccca
     2281 ctgagcacag ggtcagtggc ctcttctaaa ttcttggtcc tggctcagcc acccactgca
     2341 gtatccacat acccaagcct cactggttgt gaccactaag tcagtgacaa ccactggttt
     2401 agtaattaga tgaaggcaaa agttgtaaga caaggtaaaa atctgagatt tctgaggcac
     2461 agaataaaga gtttgggaca agactgtata tctgtgaagt agccgggtga aagaatatta
     2521 aatagaaact gatttcattc taaaaaaaaa aaaaaaaaaa aaaaaaa
//
