LOCUS       NM_023022               2160 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Rattus norvegicus Rh-associated glycoprotein (Rhag), mRNA.
ACCESSION   NM_023022
VERSION     NM_023022.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Genetet,S., Ripoche,P., Picot,J., Bigot,S., Delaunay,J.,
            Armari-Alla,C., Colin,Y. and Mouro-Chanteloup,I.
  TITLE     Human RhAG ammonia channel is impaired by the Phe65Ser mutation in
            overhydrated stomatocytic red cells
  JOURNAL   Am J Physiol Cell Physiol 302 (2), C419-C428 (2012)
   PUBMED   22012326
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Goossens,D., Trinh-Trang-Tan,M.M., Debbia,M., Ripoche,P.,
            Vilela-Lamego,C., Louache,F., Vainchenker,W., Colin,Y. and
            Cartron,J.P.
  TITLE     Generation and characterisation of Rhd and Rhag null mice
  JOURNAL   Br J Haematol 148 (1), 161-172 (2010)
   PUBMED   19807729
REFERENCE   3  (bases 1 to 2160)
  AUTHORS   Musa-Aziz,R., Chen,L.M., Pelletier,M.F. and Boron,W.F.
  TITLE     Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG
  JOURNAL   Proc Natl Acad Sci U S A 106 (13), 5406-5411 (2009)
   PUBMED   19273840
REFERENCE   4  (bases 1 to 2160)
  AUTHORS   Bruce,L.J., Guizouarn,H., Burton,N.M., Gabillat,N., Poole,J.,
            Flatt,J.F., Brady,R.L., Borgese,F., Delaunay,J. and Stewart,G.W.
  TITLE     The monovalent cation leak in overhydrated stomatocytic red blood
            cells results from amino acid substitutions in the Rh-associated
            glycoprotein
  JOURNAL   Blood 113 (6), 1350-1357 (2009)
   PUBMED   18931342
REFERENCE   5  (bases 1 to 2160)
  AUTHORS   Endeward,V., Cartron,J.P., Ripoche,P. and Gros,G.
  TITLE     RhAG protein of the Rhesus complex is a CO2 channel in the human
            red cell membrane
  JOURNAL   FASEB J 22 (1), 64-73 (2008)
   PUBMED   17712059
REFERENCE   6  (bases 1 to 2160)
  AUTHORS   Benjelloun,F., Bakouh,N., Fritsch,J., Hulin,P., Lipecka,J.,
            Edelman,A., Planelles,G., Thomas,S.R. and Cherif-Zahar,B.
  TITLE     Expression of the human erythroid Rh glycoprotein (RhAG) enhances
            both NH3 and NH4+ transport in HeLa cells
  JOURNAL   Pflugers Arch 450 (3), 155-167 (2005)
   PUBMED   15856280
REFERENCE   7  (bases 1 to 2160)
  AUTHORS   Nicolas,V., Le Van Kim,C., Gane,P., Birkenmeier,C., Cartron,J.P.,
            Colin,Y. and Mouro-Chanteloup,I.
  TITLE     Rh-RhAG/ankyrin-R, a new interaction site between the membrane
            bilayer and the red cell skeleton, is impaired by
            Rh(null)-associated mutation
  JOURNAL   J Biol Chem 278 (28), 25526-25533 (2003)
   PUBMED   12719424
REFERENCE   8  (bases 1 to 2160)
  AUTHORS   Marini,A.M., Matassi,G., Raynal,V., Andre,B., Cartron,J.P. and
            Cherif-Zahar,B.
  TITLE     The human Rhesus-associated RhAG protein and a kidney homologue
            promote ammonium transport in yeast
  JOURNAL   Nat Genet 26 (3), 341-344 (2000)
   PUBMED   11062476
REFERENCE   9  (bases 1 to 2160)
  AUTHORS   Huang,C.H., Cheng,G., Liu,Z., Chen,Y., Reid,M.E., Halverson,G. and
            Okubo,Y.
  TITLE     Molecular basis for Rh(null) syndrome: identification of three new
            missense mutations in the Rh50 glycoprotein gene
  JOURNAL   Am J Hematol 62 (1), 25-32 (1999)
   PUBMED   10467273
REFERENCE   10 (bases 1 to 2160)
  AUTHORS   Kitano,T., Sumiyama,K., Shiroishi,T. and Saitou,N.
  TITLE     Conserved evolution of the Rh50 gene compared to its homologous Rh
            blood group gene
  JOURNAL   Biochem Biophys Res Commun 249 (1), 78-85 (1998)
   PUBMED   9705835
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF531097.1.
            
            On Dec 20, 2003 this sequence version replaced NM_023022.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF531097.1, AB015194.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132278,
                                           SAMD00155320 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q13"
     gene            1..2160
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /note="Rh-associated glycoprotein"
                     /db_xref="GeneID:65207"
                     /db_xref="RGD:61871"
     exon            1..247
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1380
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /note="Rhesus blood group-associated A glycoprotein; rh
                     type A glycoprotein; rh family type A glycoprotein;
                     erythrocyte membrane glycoprotein Rh50; rhesus blood group
                     family type A glycoprotein; glycoprotein 50kD; Rhesus
                     blood group-associated A glycoprotein-like"
                     /codon_start=1
                     /product="ammonium transporter Rh type A"
                     /protein_id="NP_075411.2"
                     /db_xref="GeneID:65207"
                     /db_xref="RGD:61871"
                     /translation="MRFKFSLIALSLEVVMIVSFALFVEYETSQNGSQKSASQQNASQ
                     QNAAAQQNASQQGNASSPAKEDQFFQLYPLFQHVHVMIFVGFGFLMTFLKKYGFSGVG
                     FNLFLAALGLQWGTIVQGLLHSHGLKFPFRIKNMINADFSTATVLISFGAVLGKTSPI
                     QMIIMTILEIAVFAGNEHLVTEIFKASDTGASMTIHAFGAYFGLAVAGVLYRSGLKHG
                     HPNEESVYHSDLFAMIGTLFLWMFWPSFNSAIAQPENNQYRAIVNTYMSLAACVITAY
                     ALSSLVERRGRLDMVHIQNATLAGGVAVGTCADMEIPLYFAMTIGSIAGIISVLGYKF
                     LSPLLAHKLMIHDTCGVHNLHGLPGVFGGLASIVAISWGKSTVSTMAMQATALGSSIG
                     SAIVGGLVTGLILKLPVWNQPPDEYCFDDSVSWKVPKYRELDNYFFQHVTHNHVEHEV
                     "
     exon            248..431
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            432..582
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            583..730
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            731..897
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1035
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1157
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1231
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1305
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..2145
                     /gene="Rhag"
                     /gene_synonym="Rh50"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actgcgactc tgtcctttgc cacagtcatg aggttcaaat tctctctcat agccttaagc
       61 ctggaagttg ttatgattgt ttcatttgca ttatttgttg aatatgagac atcccagaat
      121 ggttcccaga agagtgcttc ccagcagaat gcttcccagc agaatgctgc tgcccagcag
      181 aatgcgtccc aacaggggaa tgcctcaagt ccagcaaaag aggaccaatt ttttcagttg
      241 tatcctttat tccaacatgt gcatgtcatg atatttgttg ggtttggctt cctcatgacc
      301 ttcctgaaga aatatggttt cagtggtgta ggttttaacc tctttcttgc tgctctgggt
      361 ctccaatggg gtacgattgt acagggcctt cttcacagcc acggactaaa atttcccttc
      421 agaatcaaaa atatgataaa cgcagacttc agtacagcca cagttctaat ttcctttggt
      481 gctgtcctgg ggaaaacaag ccccatccaa atgataatca tgacaattct ggaaattgct
      541 gtatttgctg gcaatgaaca tctggttact gaaatattta aggcctctga cactggggcg
      601 tcaatgacaa tccatgcctt tggagcctac tttggcttag cggtagcagg cgtcttatac
      661 cggtctggcc tcaaacatgg acacccaaat gaagaatctg tgtaccactc tgacttgttt
      721 gcaatgatcg gaacactttt cctgtggatg ttttggccca gctttaattc agccattgct
      781 caacctgaaa ataatcagta tagggccatt gtcaacacat acatgtccct tgctgcctgt
      841 gtgatcacag cctatgcctt gtccagcctt gttgagcgcc gaggcaggct ggatatggta
      901 cacattcaga atgctactct agcaggaggt gtggctgtgg gcacatgtgc agacatggaa
      961 atccccctat attttgctat gaccattgga agcattgcag ggatcatctc agtgcttgga
     1021 tacaagttcc ttagtccact cttagctcat aaactgatga tacacgatac atgtggggtt
     1081 cataacttgc acggcttacc cggtgtgttt ggaggccttg ccagcattgt ggccataagc
     1141 tgggggaagt ctacagtgtc cactatggct atgcaggcaa cagcactggg atcttccatt
     1201 ggctcagcaa ttgttggagg tttggttaca ggtctcattc taaagctacc tgtctggaac
     1261 cagccacctg acgagtactg ctttgatgat tctgtttctt ggaaggttcc caaatacaga
     1321 gaactggata attacttctt tcaacacgtg actcacaacc atgtggaaca cgaagtctaa
     1381 ataccattcc taaactcagc atcctcagac ttaccaggaa gagtcaagtt taaagaaacc
     1441 aacgcttggt atacagagag ataggcatgg caatataaaa tataaataca aaagatgcct
     1501 ttagttttgg tattattcct tgatgttcaa atgatttctc agtcgacaga aaattgctca
     1561 caaagcagtc tatcctagac cctaaggatg actgcaaacg aagaggttcc taatgaatgt
     1621 ttgattgtat tcttgataat ttgcaaaaga gatgaatcat agaaatcaat atttcttgat
     1681 catatcagac tttatccact ttttaggtga gttttcattg tggtatcttt gtgcatgtgt
     1741 gtcatagccc tacccctatc cactgcagtc cctcaagatt ctttgccccc tccagctggg
     1801 tccctttctt ccccgactta ggtatttctt gttctattac atgcatttta tctgcaaccc
     1861 tttagcttct taatgtacat gtcttcagtt ccaaagagta taaagctgaa gtcaaaggac
     1921 atattaaaga atctattcta gaaaaatgaa ataaatctat gtaaataaaa ggtaatatta
     1981 atgttctagt tagcatcgtt ttaaatctta aaaacagtca cagtaatgtt acactgttgt
     2041 ttgcatattc ttatccttga taccttctta tggttattat ttgtaataag agtttaaagg
     2101 ccacctgtat aaattttcaa atgattaaat attatcttaa aataaaaaaa aaaaaaaaaa
//
