LOCUS       NM_053764               2857 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus regulator of G-protein signaling 14 (Rgs14),
            mRNA.
ACCESSION   NM_053764
VERSION     NM_053764.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Gerber,K.J., Squires,K.E. and Hepler,J.R.
  TITLE     14-3-3gamma binds regulator of G protein signaling 14 (RGS14) at
            distinct sites to inhibit the RGS14:Galphai-AlF4- signaling complex
            and RGS14 nuclear localization
  JOURNAL   J Biol Chem 293 (38), 14616-14631 (2018)
   PUBMED   30093406
  REMARK    GeneRIF: findings describe a novel mechanism of negative regulation
            of RGS14 functions, specifically interactions with active Galphai
            and nuclear import, while leaving the function of other RGS14
            domains intact
            Erratum:[J Biol Chem. 2019 Aug 9;294(32):12262. doi:
            10.1074/jbc.AAC119.101131.. PMID: 31399537]
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Squires,K.E., Gerber,K.J., Pare,J.F., Branch,M.R., Smith,Y. and
            Hepler,J.R.
  TITLE     Regulator of G protein signaling 14 (RGS14) is expressed pre- and
            postsynaptically in neurons of hippocampus, basal ganglia, and
            amygdala of monkey and human brain
  JOURNAL   Brain Struct Funct 223 (1), 233-253 (2018)
   PUBMED   28776200
REFERENCE   3  (bases 1 to 2857)
  AUTHORS   Branch,M.R. and Hepler,J.R.
  TITLE     Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that
            localizes to juxtanuclear membranes and chromatin-rich regions of
            the nucleus
  JOURNAL   PLoS One 12 (9), e0184497 (2017)
   PUBMED   28934222
  REMARK    GeneRIF: Results show that endogenous RGS14 localizes to
            subcellular compartments not previously recognized in studies of
            recombinant RGS14 in unnatural host cells. The two most surprising
            observations were that RGS14 is not enriched at the plasma
            membrane, and that it is found within chromatin-rich regions of the
            nucleus in close proximity with active RNA Pol-II.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2857)
  AUTHORS   Vellano,C.P., Brown,N.E., Blumer,J.B. and Hepler,J.R.
  TITLE     Assembly and function of the regulator of G protein signaling 14
            (RGS14).H-Ras signaling complex in live cells are regulated by
            Galphai1 and Galphai-linked G protein-coupled receptors
  JOURNAL   J Biol Chem 288 (5), 3620-3631 (2013)
   PUBMED   23250758
  REMARK    GeneRIF: Inactive Galpha(i1)-GDP enhances the affinity of RGS14 for
            H-Ras-GTP in live cells, resulting in a ternary signaling complex
            that is further regulated by G protein-coupled receptors.
REFERENCE   5  (bases 1 to 2857)
  AUTHORS   Urabe,Y., Tanikawa,C., Takahashi,A., Okada,Y., Morizono,T.,
            Tsunoda,T., Kamatani,N., Kohri,K., Chayama,K., Kubo,M., Nakamura,Y.
            and Matsuda,K.
  TITLE     A genome-wide association study of nephrolithiasis in the Japanese
            population identifies novel susceptible Loci at 5q35.3, 7p14.3, and
            13q14.1
  JOURNAL   PLoS Genet 8 (3), e1002541 (2012)
   PUBMED   22396660
REFERENCE   6  (bases 1 to 2857)
  AUTHORS   Hollinger,S., Ramineni,S. and Hepler,J.R.
  TITLE     Phosphorylation of RGS14 by protein kinase A potentiates its
            activity toward G alpha i
  JOURNAL   Biochemistry 42 (3), 811-819 (2003)
   PUBMED   12534294
  REMARK    GeneRIF: Substituting protein kinase A phosphorylation site Thr494
            with either aspartate or glutamate to mimic phosphorylation
            enhances RGS14 inhibition of nucleotide exchange activity on G
            alpha(i) subunits almost 3-fold.
REFERENCE   7  (bases 1 to 2857)
  AUTHORS   Kimple,R.J., Kimple,M.E., Betts,L., Sondek,J. and Siderovski,D.P.
  TITLE     Structural determinants for GoLoco-induced inhibition of nucleotide
            release by Galpha subunits
  JOURNAL   Nature 416 (6883), 878-881 (2002)
   PUBMED   11976690
  REMARK    GeneRIF: Structural determinants for GoLoco-induced inhibition of
            nucleotide release by Galpha subunits
REFERENCE   8  (bases 1 to 2857)
  AUTHORS   Kimple,R.J., De Vries,L., Tronchere,H., Behe,C.I., Morris,R.A.,
            Gist Farquhar,M. and Siderovski,D.P.
  TITLE     RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with
            guanine nucleotide dissociation inhibitor Activity
  JOURNAL   J Biol Chem 276 (31), 29275-29281 (2001)
   PUBMED   11387333
REFERENCE   9  (bases 1 to 2857)
  AUTHORS   Traver,S., Bidot,C., Spassky,N., Baltauss,T., De Tand,M.F.,
            Thomas,J.L., Zalc,B., Janoueix-Lerosey,I. and Gunzburg,J.D.
  TITLE     RGS14 is a novel Rap effector that preferentially regulates the
            GTPase activity of galphao
  JOURNAL   Biochem J 350 Pt 1 (Pt 1), 19-29 (2000)
   PUBMED   10926822
REFERENCE   10 (bases 1 to 2857)
  AUTHORS   Snow,B.E., Antonio,L., Suggs,S., Gutstein,H.B. and Siderovski,D.P.
  TITLE     Molecular cloning and expression analysis of rat Rgs12 and Rgs14
  JOURNAL   Biochem Biophys Res Commun 233 (3), 770-777 (1997)
   PUBMED   9168931
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Nov 26, 2020 this sequence version replaced NM_053764.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U92279.1, SRR26360176.828555.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                JAXUCZ010000017.1  9268149-9268233     c
            86-309              JAXUCZ010000017.1  9267732-9267955     c
            310-331             JAXUCZ010000017.1  9261222-9261243     c
            332-510             JAXUCZ010000017.1  9260794-9260972     c
            511-603             JAXUCZ010000017.1  9260390-9260482     c
            604-747             JAXUCZ010000017.1  9260163-9260306     c
            748-891             JAXUCZ010000017.1  9259721-9259864     c
            892-994             JAXUCZ010000017.1  9259490-9259592     c
            995-1107            JAXUCZ010000017.1  9259097-9259209     c
            1108-1311           JAXUCZ010000017.1  9258135-9258338     c
            1312-1388           JAXUCZ010000017.1  9256327-9256403     c
            1389-1515           JAXUCZ010000017.1  9256003-9256129     c
            1516-1597           JAXUCZ010000017.1  9255830-9255911     c
            1598-1644           JAXUCZ010000017.1  9255675-9255721     c
            1645-1759           JAXUCZ010000017.1  9255479-9255593     c
            1760-2857           JAXUCZ010000017.1  9254149-9255246     c
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     gene            1..2857
                     /gene="Rgs14"
                     /note="regulator of G-protein signaling 14"
                     /db_xref="GeneID:114705"
                     /db_xref="RGD:620003"
     exon            1..85
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            86..309
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..1899
                     /gene="Rgs14"
                     /codon_start=1
                     /product="regulator of G-protein signaling 14"
                     /protein_id="NP_446216.1"
                     /db_xref="GeneID:114705"
                     /db_xref="RGD:620003"
                     /translation="MPGKPKHLGVPNGRMVLAVSDGELTSTSGSQAQGEGRGSSLSIH
                     SLPSGPSSPFSTDEQPVASWAQSFERLLQDPRGLAYFTEFLKKEFSAENVTFWQACER
                     FQQIPASDTKQLAQEAHNIYHEFLSSQALSPVNIDRQAWLSEEVLAQPRPDMFRAQQL
                     QIFNLMKFDSYARFVKSPLYQECLLAEAEGRPLREPGSSHLGSPDTARKKPKLKPGKS
                     LPLGVEELGQLPLAEGRPLRKSFRREMPGGAVNSALRRESQGSLNSSASLDLGFLAFV
                     SSKSESHRKSLGSGEGESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRG
                     LSLPDIKVYLVGKEQKALVLDQDCTVLADQEVRLENRITFQLELVGLERVVRISAKPT
                     KRLQEALQPILAKHGLSLDQVVLHRPGEKQLVDLENLVSSVASQTLVLDTLPDAKTRE
                     ASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTGKRQTCDIEGLVELLNRVQ
                     SSGAHDQRGLLRKEDLVLPEFLQLPSQRPGSQEAPP"
     exon            310..331
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            332..510
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            511..603
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            604..747
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            748..891
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            892..994
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1107
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1311
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1388
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1515
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1597
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1644
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1759
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..2857
                     /gene="Rgs14"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcacttctgt gtgctgacac atccttggag ccttcagact gagccagact ccaggctcct
       61 cgctgggcac aaggaggctc ctgctagaca cttcctgtgg cagagaaaag aggtagtgag
      121 ctggtgtttc aggatgtaag ggcccggagg agcagagcga gctctctcca ctgcttgccc
      181 gccaccctga gagtgctggt cggtgtttcc acaatccttg tggtgggtgg ccccccgtgg
      241 aggggacctg tgagcaacgg aggcatgcca gggaagccca agcacttggg tgtccccaac
      301 gggcgcatgg tcctggctgt ctctgatgga gaactgacca gcacatcagg ttcccaggcc
      361 cagggtgagg gcagaggcag ctccctcagc atccacagcc tccccagtgg ccccagcagc
      421 cccttctcca ctgatgagca gcctgtggcc agctgggccc agtcctttga gcggctgctg
      481 caagacccac ggggtctggc ttacttcact gagttcctga agaaggaatt cagcgccgag
      541 aatgtaactt tctggcaagc ctgcgagcgt ttccaacaga tcccagccag cgacaccaaa
      601 cagctagctc aggaggccca caacatctac catgagttcc tgtccagcca ggcactgagc
      661 ccagtgaaca tcgaccgaca ggcctggctt agtgaggagg tgctggccca gcccaggccc
      721 gatatgttcc gagcacagca gcttcagatc ttcaatttga tgaagttcga cagctacgcg
      781 cgcttcgtca aatccccgct gtaccaagag tgcctgctgg ccgaggccga gggacgcccc
      841 ctgcgggaac ctggatcctc acacctcggg agcccggaca ctgcgaggaa gaagccaaag
      901 ctgaagcctg gaaagtcact gccgctcggt gtggaagagt tggggcagct gccgcttgct
      961 gagggccgcc ctctccgcaa gtcctttcgt agagagatgc cgggtggagc tgtgaattcg
     1021 gccctgcgac gagagtctca gggatccctg aattcttccg cgagtctgga cctgggcttc
     1081 cttgcctttg tgagcagcaa atccgagagc caccgaaaga gccttggaag tggagagggt
     1141 gagagcgaaa gccggcccgg gaagtactgc tgcgtgtatc tacctgatgg cacggcctcc
     1201 ttggccctgg ctcgacctgg cctcaccatc cgtgacatgc tggcaggcat ctgtgagaag
     1261 agaggcctct ctctacctga cattaaggtc tacctggtgg gcaaagaaca gaaggccctg
     1321 gtcctggatc aggactgcac cgtgctggca gaccaggaag tgcgactgga aaacaggatc
     1381 accttccagc tggagttggt cggcctggag cgtgtggtac ggatctctgc taagcccacc
     1441 aagcgtctgc aggaggcgct gcagcctatc ttggccaagc acggcctgag cctggatcag
     1501 gtggtcctac acaggccagg agagaagcag ctagtggatt tggagaatct agtgagctca
     1561 gtggcttcac agacactggt tttggacact cttccagatg caaagacaag ggaagccagc
     1621 agcatacctc cctgccgcag ccagggatgc ctccctagaa cccagaccaa ggacagtcac
     1681 cttcctccac tgtcctccag tttgtcggta gaagatgcca gtggttctac tgggaaacga
     1741 cagacctgcg acattgaagg cctagtggag ctgctgaatc gtgtgcagag cagcggggcc
     1801 catgaccaga ggggacttct tcgcaaagag gacctggtcc ttccagaatt tctgcagctt
     1861 ccttcccaaa gaccaggttc tcaggaggct ccaccataga ctgaatcaac tattcagccc
     1921 agggagggcc cttcagactc caccatccac tcggtcctct gacacctgtg taacaatcca
     1981 ggctgtctgc atggtgcctg ggcaaaccaa gcatgccacg ggtctgtgct gcatgccctg
     2041 tctgtaccag gagtgtcttt ggtccctttc tgctgtgggc aggcccacag aaagggccag
     2101 gaaggggtgg ctacttgttc tctcataggt ttacctacca attcctcaca gtaggccatc
     2161 aaggggaggt gggccttgtc ccttggcata agcatactga acatgaagag gtgactttgg
     2221 cagtgtcagc ctccttgact tgttctaggc tttagcaggg ggcaggaagg aggggctggc
     2281 ccctccttaa agagttggtg taagaaagac ctgggtgtac tactgggcaa cccctccctc
     2341 catccacaga ctctgctgta catatggatt ttatggttgg cttggggaag ctgggtttgt
     2401 cctggatgta tgatactgtt ataataataa ttattattat tcagccacga ggtgtctttt
     2461 ttttttcttt tttttttcgg agctgggaac cgaacccagg gccttgcgct tgctaggcaa
     2521 gcgctctacc actgagctaa atccccaacc ccatgaggtg tctttcttgt atgccactct
     2581 gccctgtgta gtctcactac cccactgcct ctgtgccagg ccaggaagtt ttggggaacc
     2641 ttagtgtata cctcctcact tcttgttttc agaatctctc tccccaaggc ataggatata
     2701 gaaggcaggg agggggggga gcagaatgga gagggagaga gcgccacccc ccacccccta
     2761 ctgttcttgg ccatcaccca gctgctccac aatgtccatg ggagatgaat gtatcctttt
     2821 tgtttctgtt ctgtttctgt tggtgataca atgtatc
//
