LOCUS       NP_062212               1387 aa            linear   ROD 01-MAY-2025
DEFINITION  regulator of G-protein signaling 12 [Rattus norvegicus].
ACCESSION   NP_062212
VERSION     NP_062212.2
DBSOURCE    REFSEQ: accession NM_019339.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1387)
  AUTHORS   Lan,T., Li,Y., Fan,C., Wang,L., Wang,W., Chen,S. and Yu,S.Y.
  TITLE     MicroRNA-204-5p reduction in rat hippocampus contributes to
            stress-induced pathology via targeting RGS12 signaling pathway
  JOURNAL   J Neuroinflammation 18 (1), 243 (2021)
   PUBMED   34674723
  REMARK    GeneRIF: MicroRNA-204-5p reduction in rat hippocampus contributes
            to stress-induced pathology via targeting RGS12 signaling pathway.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1387)
  AUTHORS   Willard,M.D., Willard,F.S., Li,X., Cappell,S.D., Snider,W.D. and
            Siderovski,D.P.
  TITLE     Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth
            factor-mediated differentiation
  JOURNAL   EMBO J 26 (8), 2029-2040 (2007)
   PUBMED   17380122
  REMARK    GeneRIF: RGS12 may play a critical, and receptor-selective, role in
            coordinating Ras-dependent signals that are required for promoting
            and/or maintaining neuronal differentiation.
REFERENCE   3  (residues 1 to 1387)
  AUTHORS   Lopez-Aranda,M.F., Acevedo,M.J., Carballo,F.J., Gutierrez,A. and
            Khan,Z.U.
  TITLE     Localization of the GoLoco motif carrier regulator of G-protein
            signalling 12 and 14 proteins in monkey and rat brain
  JOURNAL   Eur J Neurosci 23 (11), 2971-2982 (2006)
   PUBMED   16819986
REFERENCE   4  (residues 1 to 1387)
  AUTHORS   Pi,M., Spurney,R.F., Tu,Q., Hinson,T. and Quarles,L.D.
  TITLE     Calcium-sensing receptor activation of rho involves filamin and
            rho-guanine nucleotide exchange factor
  JOURNAL   Endocrinology 143 (10), 3830-3838 (2002)
   PUBMED   12239094
REFERENCE   5  (residues 1 to 1387)
  AUTHORS   Kimple,R.J., De Vries,L., Tronchere,H., Behe,C.I., Morris,R.A.,
            Gist Farquhar,M. and Siderovski,D.P.
  TITLE     RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with
            guanine nucleotide dissociation inhibitor Activity
  JOURNAL   J Biol Chem 276 (31), 29275-29281 (2001)
   PUBMED   11387333
REFERENCE   6  (residues 1 to 1387)
  AUTHORS   Schiff,M.L., Siderovski,D.P., Jordan,J.D., Brothers,G., Snow,B., De
            Vries,L., Ortiz,D.F. and Diverse-Pierluissi,M.
  TITLE     Tyrosine-kinase-dependent recruitment of RGS12 to the N-type
            calcium channel
  JOURNAL   Nature 408 (6813), 723-727 (2000)
   PUBMED   11130074
REFERENCE   7  (residues 1 to 1387)
  AUTHORS   Snow,B.E., Hall,R.A., Krumins,A.M., Brothers,G.M., Bouchard,D.,
            Brothers,C.A., Chung,S., Mangion,J., Gilman,A.G., Lefkowitz,R.J.
            and Siderovski,D.P.
  TITLE     GTPase activating specificity of RGS12 and binding specificity of
            an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain
  JOURNAL   J Biol Chem 273 (28), 17749-17755 (1998)
   PUBMED   9651375
REFERENCE   8  (residues 1 to 1387)
  AUTHORS   Snow,B.E., Antonio,L., Suggs,S., Gutstein,H.B. and Siderovski,D.P.
  TITLE     Molecular cloning and expression analysis of rat Rgs12 and Rgs14
  JOURNAL   Biochem Biophys Res Commun 233 (3), 770-777 (1997)
   PUBMED   9168931
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Mar 15, 2021 this sequence version replaced NP_062212.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: U92280.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA5760383,
                                    SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     Protein         1..1387
                     /product="regulator of G-protein signaling 12"
                     /function="GTPase activating protein for G-alpha subunits"
                     /note="regulator of G-protein signalling 12"
                     /calculated_mol_wt=150410
     Region          20..95
                     /region_name="PDZ_RGS12-like"
                     /note="PDZ domain of regulator of G-protein signaling 12
                     (RGS12), and related domains; cd06710"
                     /db_xref="CDD:467194"
     Site            order(30..36,44,47,76,80,83..84)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467194"
     Site            171
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Site            194
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8CGE9; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Region          227..358
                     /region_name="PTB_RGS12"
                     /note="Regulator of G-protein signaling 12
                     Phosphotyrosine-binding (PTB) PH-like fold; cd13162"
                     /db_xref="CDD:269984"
     Site            order(232,309,340)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269984"
     Site            order(298..303,316,351,355)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269984"
     Region          409..428
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Region          442..488
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Site            524
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0000250|UniProtKB:O14924; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Region          620..650
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Site            633
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0000250|UniProtKB:Q8CGE9; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Site            661
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Site            671
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Region          716..830
                     /region_name="RGS_RGS12"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the RGS12 protein; cd08742"
                     /db_xref="CDD:188696"
     Site            order(736..738,740..741,778..783,785..786,813,815..817,
                     821)
                     /site_type="other"
                     /note="G-alpha-3 interaction site [polypeptide binding]"
                     /db_xref="CDD:188696"
     Region          836..953
                     /region_name="RGS12_us1"
                     /note="Unstructured region of RGS12; pfam16613"
                     /db_xref="CDD:465199"
     Region          842..934
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Site            850
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8CGE9; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Site            879
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Site            943
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8CGE9; propagated from
                     UniProtKB/Swiss-Prot (O08774.1)"
     Region          962..1031
                     /region_name="RBD1_RGS12"
                     /note="Ras-binding domain (RBD) 1 of regulator of G
                     protein signaling 12 (RGS12); cd17136"
                     /db_xref="CDD:340656"
     Site            order(967,988,990,1011)
                     /site_type="other"
                     /note="key conserved lysines"
                     /db_xref="CDD:340656"
     Region          1032..1104
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          1103..1168
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Region          1106..1180
                     /region_name="RGS12_us2"
                     /note="Unstructured region between RBD and GoLoco;
                     pfam16611"
                     /db_xref="CDD:435461"
     Region          1188..1209
                     /region_name="GoLoco"
                     /note="LGN motif, putative GEFs specific for G-alpha
                     GTPases; smart00390"
                     /db_xref="CDD:214645"
     Region          1224..1325
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     Region          1238..1387
                     /region_name="RGS12_usC"
                     /note="C-terminal unstructured region of RGS12; pfam16612"
                     /db_xref="CDD:465198"
     Region          1349..1387
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O08774.1)"
     CDS             1..1387
                     /gene="Rgs12"
                     /coded_by="NM_019339.2:257..4420"
                     /db_xref="GeneID:54292"
                     /db_xref="RGD:3564"
ORIGIN      
        1 myragepgkr qsgpapprvr svevargrag ygftlsgqap cvlscvmrgs padfvglrag
       61 dqilainein vkkashedvv kligkcsgvl rmvisegssh vepsssdeeg glcegkgwlr
      121 pkldskalgi nraervveev qsggifnmif espslcasgs eplklkqrsl sesaalrldv
      181 gqdslctphp smlskeeisk vinddsvftv gldnhddfgl dasilnvamv vgylgsielp
      241 stssnlehds lqairgcmrr lraeqkihsl vtmkvmhdcv qlvtdragvv aeypaeklaf
      301 savcpddrrf fglvtmqtnd dgclaqedeg alrtschvfm vdpdlfhhki hqgiarrfgf
      361 actadpdtsg clefpasslp vlqfisvlyr dmgeliegvr arafldgdad ahqnnstssn
      421 sdsgignfnq eeksnrvlvv dlgggssrhg qgsspgwesv sgrgsqpwsa pwngtfchds
      481 eagspletsp ntdrfwdltk hsgpvfhmev ppatlrssip pskrgatgss cgfnqrwlpv
      541 hvlqewqcgh asdqesytds tdgwssvncg tlpppmskip adryrvegsf aqaplstqkr
      601 dwsrkafgmq nlfgphrnvr ktkedkkssk lgrgvalaqt sqrtsarrsf grsrrfsltr
      661 slddlesatv sdgeltgadl kdcisnnsls snaslpsvqs crrlrerrva swavsferll
      721 qdpvgvryfs dflrkefsee nilfwqacec fshvpahdkk elsyrareif skflcskatt
      781 pvnidsqaql addilnaphp dmfkeqqlqi fnlmkfdsyt rflksqlyqe cvlaevegrt
      841 lpdsqqvpss paskhsissd hsnvstpkkl sgksksgrsl nedvgeedse kkrkgaffsw
      901 srsrstgrsq kkkdhgdhah dalhangglc rresqgsvss agsldlseac rtsalerdka
      961 akhccvhlpd gtscvvavks gfsikeilsg lcerhginga avdlflvggd kplvlhqdss
     1021 ilatrdlrle krtlfrldlv pinrsvglka kptkpvtevl rpvvakygld lgsllvrlsg
     1081 ekepldlgap issldgqrvi leerdpsrgk vstekqkgap vkqssavnss prnhsamgee
     1141 rtlgksnsik irgengksar dprlskrees iakigkkkyq kinldeaeef feliskaqsn
     1201 raddqrgllr kedlvlpefl rlppgssela lsspppvkgf skravtshgq egavqteesy
     1261 sdspatspas aqspcsaysp gsahspgsah spgsahstpg ppgtaqpgek ptkpscistv
     1321 qegttqawrr lspeleaggi qtveeeqvad ltlmgegdis spnstllppp plpqdtpgpt
     1381 rpgtsrf
//
