LOCUS       NM_031546               1600 bp    mRNA    linear   ROD 07-APR-2024
DEFINITION  Rattus norvegicus regucalcin (Rgn), mRNA.
ACCESSION   NM_031546
VERSION     NM_031546.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Arakawa,K., Inoue,H., Ishigami,A., Sato,A., Takino,Y., Tanaka,M.,
            Morimoto,H., Takahashi,N. and Uehara,M.
  TITLE     Release of SMP30 in Extracellular Vesicles under Conditions of
            Ascorbic Acid Deficiency Is Involved with Acute Phase Response in
            ODS Rat
  JOURNAL   J Nutr Sci Vitaminol (Tokyo) 69 (6), 420-427 (2023)
   PUBMED   38171814
  REMARK    GeneRIF: Release of SMP30 in Extracellular Vesicles under
            Conditions of Ascorbic Acid Deficiency Is Involved with Acute Phase
            Response in ODS Rat.
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Singh,T., Banerjee,P., Uditi, Kumari,S., Chopra,A., Singh,N. and
            Qamar,I.
  TITLE     Expression of Regucalcin, a calcium-binding protein is regulated by
            hypoxia-inducible factor-1alpha
  JOURNAL   Life Sci 292, 120278 (2022)
   PUBMED   35041836
  REMARK    GeneRIF: Expression of Regucalcin, a calcium-binding protein is
            regulated by hypoxia-inducible factor-1alpha.
REFERENCE   3  (bases 1 to 1600)
  AUTHORS   Yamaguchi,M. and Murata,T.
  TITLE     Extracellular regucalcin suppresses colony formation and growth
            independent of tumor suppressor p53 in human mammary epithelial
            cells
  JOURNAL   Tissue Cell 67, 101447 (2020)
   PUBMED   33137709
  REMARK    GeneRIF: Extracellular regucalcin suppresses colony formation and
            growth independent of tumor suppressor p53 in human mammary
            epithelial cells.
REFERENCE   4  (bases 1 to 1600)
  AUTHORS   Bin-Jaliah,I. and Sakr,H.F.
  TITLE     Melatonin ameliorates brain oxidative stress and upregulates
            senescence marker protein-30 and osteopontin in a rat model of
            vascular dementia
  JOURNAL   Physiol Int 105 (1), 38-52 (2018)
   PUBMED   29602294
  REMARK    GeneRIF: Melatonin may produce a neuroprotective effect through its
            antioxidant action and by increasing hippocampal expression of
            SMP30 and osteopontin.
REFERENCE   5  (bases 1 to 1600)
  AUTHORS   Hou,J.Y., Wang,Y.G., Ma,S.J., Yang,B.Y. and Li,Q.P.
  TITLE     Identification of a prognostic 5-Gene expression signature for
            gastric cancer
  JOURNAL   J Cancer Res Clin Oncol 143 (4), 619-629 (2017)
   PUBMED   28035468
REFERENCE   6  (bases 1 to 1600)
  AUTHORS   Fujita,T., Shirasawa,T., Uchida,K. and Maruyama,N.
  TITLE     Isolation of cDNA clone encoding rat senescence marker protein-30
            (SMP30) and its tissue distribution
  JOURNAL   Biochim Biophys Acta 1132 (3), 297-305 (1992)
   PUBMED   1420310
  REMARK    Erratum:[Biochim Biophys Acta 1992 Dec 29;1171(2):235]
REFERENCE   7  (bases 1 to 1600)
  AUTHORS   Yamaguchi,M. and Tai,H.
  TITLE     Calcium-binding protein regucalcin increases calcium-independent
            proteolytic activity in rat liver cytosol
  JOURNAL   Mol Cell Biochem 112 (1), 89-95 (1992)
   PUBMED   1513338
REFERENCE   8  (bases 1 to 1600)
  AUTHORS   Yamaguchi,M. and Sakurai,T.
  TITLE     Reversible effect of calcium-binding protein regucalcin on the
            Ca(2+)-induced inhibition of deoxyuridine 5'-triphosphatase
            activity in rat liver cytosol
  JOURNAL   Mol Cell Biochem 110 (1), 25-29 (1992)
   PUBMED   1315924
REFERENCE   9  (bases 1 to 1600)
  AUTHORS   Yamaguchi,M. and Tai,H.
  TITLE     Inhibitory effect of calcium-binding protein regucalcin on
            Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase
            activity in rat liver cytosol
  JOURNAL   Mol Cell Biochem 106 (1), 25-30 (1991)
   PUBMED   1656206
REFERENCE   10 (bases 1 to 1600)
  AUTHORS   Yamaguchi,M. and Yamamoto,T.
  TITLE     Purification of calcium binding substance from soluble fraction of
            normal rat liver
  JOURNAL   Chem Pharm Bull (Tokyo) 26 (6), 1915-1918 (1978)
   PUBMED   699201
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Nov 26, 2020 this sequence version replaced NM_031546.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB037934.1, X69021.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760400,
                                           SAMEA5760476 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                JAXUCZ010000021.1  4187876-4187957     c
            83-261              JAXUCZ010000021.1  4187183-4187361     c
            262-444             JAXUCZ010000021.1  4185065-4185247     c
            445-660             JAXUCZ010000021.1  4176696-4176911     c
            661-792             JAXUCZ010000021.1  4174670-4174801     c
            793-947             JAXUCZ010000021.1  4174054-4174208     c
            948-1600            JAXUCZ010000021.1  4172537-4173189     c
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq11"
     gene            1..1600
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /note="regucalcin"
                     /db_xref="GeneID:25106"
                     /db_xref="RGD:3560"
     exon            1..82
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     exon            83..261
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..998
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /EC_number="3.1.1.17"
                     /note="senescence marker protein 30; SMP-30;
                     gluconolactonase; regucalcin (senescence marker
                     protein-30)"
                     /codon_start=1
                     /product="regucalcin"
                     /protein_id="NP_113734.1"
                     /db_xref="GeneID:25106"
                     /db_xref="RGD:3560"
                     /translation="MSSIKIECVLRENYRCGESPVWEEASKCLLFVDIPSKTVCRWDS
                     ISNRVQRVGVDAPVSSVALRQSGGYVATIGTKFCALNWEDQSVFILAMVDEDKKNNRF
                     NDGKVDPAGRYFAGTMAEETAPAVLERHQGSLYSLFPDHSVKKYFDQVDISNGLDWSL
                     DHKIFYYIDSLSYTVDAFDYDLPTGQISNRRTVYKMEKDEQIPDGMCIDVEGKLWVAC
                     YNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGMSAEGLLRQP
                     DAGNIFKITGLGVKGIAPYSYAG"
     exon            262..444
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     exon            445..660
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     exon            661..792
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     exon            793..947
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1600
                     /gene="Rgn"
                     /gene_synonym="GNL; Rc; Reguc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcattaca acccaggtgt ggatgctgga gtgtttcctt tgtcttctat tttaaagata
       61 tcttgaaaaa aaactgtcac tgtccttttc ctgcgaccat gtcttccatc aagattgaat
      121 gtgttttaag ggagaactac aggtgtgggg agtcccctgt gtgggaggag gcatcaaagt
      181 gtctgctgtt tgtagacatc ccttcaaaga ctgtctgccg atgggattcg atcagcaatc
      241 gagtgcagcg agttggtgta gatgccccag tcagttcagt ggcacttcga cagtcaggag
      301 gctatgttgc caccattgga accaagttct gtgctttgaa ctgggaagat caatcagtat
      361 ttatcctagc catggtggat gaagataaga aaaacaatcg attcaatgat gggaaggtgg
      421 atcctgctgg gagatacttt gctggtacca tggctgagga aacggcccca gctgttctgg
      481 agcggcacca agggtccttg tactcccttt ttcctgatca cagtgtgaag aaatactttg
      541 accaagtgga tatctccaat ggtttggatt ggtccctgga ccataaaatc ttctactaca
      601 ttgacagcct gtcctacact gtggatgcct ttgactatga cctgccaaca ggacagattt
      661 ccaaccgcag aactgtttac aagatggaaa aagatgaaca aatcccagat ggaatgtgca
      721 ttgatgttga ggggaagctt tgggtggcct gttacaatgg aggaagagta attcgcctag
      781 atcctgagac agggaaaaga ctgcaaactg tgaagttgcc tgttgataaa acaacttcat
      841 gctgctttgg agggaaggat tactctgaaa tgtacgtgac atgtgccagg gatgggatga
      901 gcgccgaagg tcttttgagg cagcctgatg ctggtaacat tttcaagata acaggtcttg
      961 gggtcaaagg aattgctcca tattcctatg cagggtaaac tgcagctctt ccttgctgtc
     1021 agaagaaaaa gctttgaaga caactggaga attaagggag agaaatcaat gaactttcaa
     1081 tcttgttttt ttaatgaggc agtgatatta tagcatggtt aaactgcttt aatttacact
     1141 tttgattggg tgctggggaa taaacctaaa gcatggcata ttaatgaaga acatatggta
     1201 accatgaact ccatctctgg attcctttat ttacaatttt ttaaaggttg aatattcgca
     1261 ccagagaatg acaagtggtt ttgacaacat actctaggcc ttctattaaa aacaatctgt
     1321 agaaaaggag ctcaactatc aatcatcctg atcaattgct ctggggcttg aaatgagaca
     1381 atttccattc ttttttttgc attccatact tgctgtacat aaggcttcca aaaaatcaac
     1441 gcatagaaac ttagttgtaa tttataatga ttgtgtgact tttcatgtgt tattttacaa
     1501 actaaaatat taccagatgt tattcaaaca ctggagcaca aactgtggtt ttaactgcca
     1561 cctagtggct atacaaataa atgcatcaca ttacaaagaa
//
