LOCUS       NM_001402202            4307 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus ring finger and WD repeat domain 3 (Rfwd3),
            transcript variant 2, mRNA.
ACCESSION   NM_001402202 XM_341690
VERSION     NM_001402202.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Knies,K., Inano,S., Ramirez,M.J., Ishiai,M., Surralles,J.,
            Takata,M. and Schindler,D.
  TITLE     Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi
            anemia
  JOURNAL   J Clin Invest 127 (8), 3013-3027 (2017)
   PUBMED   28691929
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Inano,S., Sato,K., Katsuki,Y., Kobayashi,W., Tanaka,H.,
            Nakajima,K., Nakada,S., Miyoshi,H., Knies,K., Takaori-Kondo,A.,
            Schindler,D., Ishiai,M., Kurumizaka,H. and Takata,M.
  TITLE     RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA
            and RAD51 from DNA Damage Sites to Facilitate Homologous
            Recombination
  JOURNAL   Mol Cell 66 (5), 622-634 (2017)
   PUBMED   28575658
  REMARK    Erratum:[Mol Cell. 2020 Apr 2;78(1):192. PMID: 32243829]
REFERENCE   3  (bases 1 to 4307)
  AUTHORS   Feeney,L., Munoz,I.M., Lachaud,C., Toth,R., Appleton,P.L.,
            Schindler,D. and Rouse,J.
  TITLE     RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for
            Interstrand Crosslink Repair and Human Health
  JOURNAL   Mol Cell 66 (5), 610-621 (2017)
   PUBMED   28575657
REFERENCE   4  (bases 1 to 4307)
  AUTHORS   Elia,A.E., Wang,D.C., Willis,N.A., Boardman,A.P., Hajdu,I.,
            Adeyemi,R.O., Lowry,E., Gygi,S.P., Scully,R. and Elledge,S.J.
  TITLE     RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled
            Replication Forks
  JOURNAL   Mol Cell 60 (2), 280-293 (2015)
   PUBMED   26474068
REFERENCE   5  (bases 1 to 4307)
  AUTHORS   Gong,Z. and Chen,J.
  TITLE     E3 ligase RFWD3 participates in replication checkpoint control
  JOURNAL   J Biol Chem 286 (25), 22308-22313 (2011)
   PUBMED   21504906
REFERENCE   6  (bases 1 to 4307)
  AUTHORS   Fu,X., Yucer,N., Liu,S., Li,M., Yi,P., Mu,J.J., Yang,T., Chu,J.,
            Jung,S.Y., O'Malley,B.W., Gu,W., Qin,J. and Wang,Y.
  TITLE     RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53
            stability in response to DNA damage
  JOURNAL   Proc Natl Acad Sci U S A 107 (10), 4579-4584 (2010)
   PUBMED   20173098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Mar 8, 2022 this sequence version replaced XM_341690.9.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ230829.1, SRR26643429.56091.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                JAXUCZ010000019.1  56177746-56177815   c
            71-590              JAXUCZ010000019.1  56174759-56175278   c
            591-757             JAXUCZ010000019.1  56170907-56171073   c
            758-825             JAXUCZ010000019.1  56167311-56167378   c
            826-1011            JAXUCZ010000019.1  56164797-56164982   c
            1012-1103           JAXUCZ010000019.1  56164620-56164711   c
            1104-1218           JAXUCZ010000019.1  56159284-56159398   c
            1219-1447           JAXUCZ010000019.1  56158347-56158575   c
            1448-1598           JAXUCZ010000019.1  56155999-56156149   c
            1599-1775           JAXUCZ010000019.1  56152247-56152423   c
            1776-1990           JAXUCZ010000019.1  56150343-56150557   c
            1991-2202           JAXUCZ010000019.1  56148700-56148911   c
            2203-4307           JAXUCZ010000019.1  56145221-56147325   c
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..4307
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /note="ring finger and WD repeat domain 3"
                     /db_xref="GeneID:103690025"
                     /db_xref="RGD:1305243"
     exon            1..70
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..2346
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase RFWD3-like"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RFWD3 isoform 2"
                     /protein_id="NP_001389131.1"
                     /db_xref="GeneID:103690025"
                     /db_xref="RGD:1305243"
                     /translation="MSDLVPAVVAHEAMDCGLQVPLDGTLEQAAPTEVISHGAPLLQP
                     PPSDLVSSQGEALVQPAPADVVSSQGGLPTLQPVPPASIDLTEEVQPAEENMEIVDPG
                     TSEERSEGSGTNGAVGAAPSVSMNYFISGLQRLHTMLELLRPSSSQSDHSAGGPIRRR
                     RRTGFVSRARTGGSQRLNNARLRTFFQVSRSQPYPVASAHGSEARTPPSGTDSDSDSS
                     AENEEAAARAEEPEAAVPEQGVISTDQEAASVTGGDASPKKQSPQKPIVLPTVEDEEG
                     ETCTICLEQWTSAGDHRLSALRCGHLFGYRCIFKWLKGQTRKCPQCNKKAKHSDIVVL
                     YARSLRALDTTEQERMKSDLLSEQALRKQAELESAQCRLQLQVLMDKCTKLNSRVQDL
                     EKLMVRHQGPAGQQCSGSQALCGSCLPSSQSQRKYSSEKTFTVSQTGNCRIMAYCDGL
                     SCLVASQPSPQASFLPGFGVKMLSTANMRSSQYIPMHSKQIRGLAFSNQSKGLLLSAS
                     LDNTIKLTSLETNTVVQTYNTGRPVWSCCWCLDENNYIYAGLANGSILIYDLRNTNTY
                     VQELVPQKARCPLVSLSYLPRAAVTAFPYGGVLAGTLENASFWELKTDFCHKPHVLSL
                     EPGGFVDFQTESSTRHCLVTYRPDKSHNTVRTVLLEMSYRLDDAGEPVCSCLPVQTFL
                     GGPTCKLLTKSAIFQSPENDGSVLVCTADEASQSALVWNAGSGSLLQNLKTDEPVLDI
                     CPFETNQNSYLATLTEKIVHIYKWE"
     exon            71..590
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            591..757
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            758..825
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1011
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1103
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1218
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1447
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1598
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1775
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1990
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2202
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..4307
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgcgcggta ggttttgcgc ggtagcgacg gcagaagtcc cgctcgggat gtccgacttg
       61 gtccccgcag tggtggctca tgaagcaatg gactgcggtc ttcaggtacc attagacggt
      121 actctagaac aagccgcccc aactgaagtc atcagccatg gggcaccact tctccagcct
      181 ccgccttctg acttggtcag cagccaaggc gaagcgcttg ttcagcctgc ccctgctgac
      241 gtggtcagca gccaaggcgg actgcccact ctgcagcctg tgccaccagc atccattgat
      301 ttgacggagg aagtacagcc cgcagaggag aatatggaga ttgtcgatcc gggaacttcg
      361 gaggagcgca gtgaggggtc aggcactaac ggtgccgtcg gagcggctcc atcagtttca
      421 atgaactact tcatcagcgg gctgcagagg cttcatacca tgctggagtt gctgagacct
      481 tcatcgtcac agtcagacca cagtgcgggg gggccaataa gaaggaggag aagaacagga
      541 ttcgtttcga gggcaagaac tggagggtct cagaggctaa acaatgccag gttgagaacc
      601 ttctttcagg tgagcaggtc tcagccttac ccagtagcct ctgctcatgg ttcagaggcc
      661 aggaccccac cctcgggaac tgattccgac agcgacagct ctgcagagaa tgaagaggcc
      721 gctgcccggg cggaagagcc tgaggctgct gtcccagaac aaggagttat ctcaacagac
      781 caagaagctg catctgtcac aggaggtgac gcatccccta aaaagcagtc tccccagaag
      841 cccatcgttc tacccactgt ggaagatgag gaaggggaaa cttgcacgat ctgtttggaa
      901 cagtggacca gtgctgggga ccaccggctc tcagctttac gctgcgggca cctctttgga
      961 tatagatgca tttttaaatg gctcaaagga caaacacgaa aatgtcctca gtgcaacaag
     1021 aaagccaagc acagtgacat cgttgtcctt tatgcccggt ccctgagagc tttggacact
     1081 actgaacagg agcgcatgaa gagtgaccta ctgagtgagc aggctctccg gaagcaggcc
     1141 gagctggaat ccgcccagtg ccggctccag cttcaggtcc ttatggataa atgcacgaaa
     1201 ctcaatagcc gtgtccagga cttggagaag cttatggtgc ggcatcaggg cccagcagga
     1261 cagcagtgca gcggctccca agcactttgt gggagctgcc tgccctccag ccagagccag
     1321 cgcaaataca gctcagagaa gaccttcaca gtatctcaga cgggaaactg ccgaatcatg
     1381 gcgtattgcg atggtctgag ctgcttggtg gcatcccagc cctctcctca ggcttccttc
     1441 cttccaggct ttggtgttaa gatgctgagt actgcgaaca tgagaagcag tcagtacatc
     1501 cctatgcata gcaagcagat tcgtggacta gctttcagca atcaatccaa aggcttgctc
     1561 ctctccgcct cccttgacaa caccattaaa ctgaccagcc tagaaacaaa tactgtggtg
     1621 cagacttaca acactggacg ccctgtctgg agctgttgct ggtgtcttga cgaaaacaat
     1681 tacatctacg ctggactggc caatggttca attctgatct atgacttacg gaacacaaat
     1741 acctatgtcc aggaattagt tcctcagaaa gccagatgtc ctctggtgtc cctgtcatat
     1801 ttacccagag ctgcggtgac tgcatttcct tacggtgggg tgctggctgg aaccttggag
     1861 aatgcctcct tctgggagct aaaaacagac ttttgtcata agcctcatgt gctatccttg
     1921 gagccagggg gctttgtaga ctttcagaca gagagcagca cccgacactg tcttgtgaca
     1981 tacaggcctg ataaaagcca taacaccgtg cgaactgtgc tgttggagat gtcttacaga
     2041 ctggatgacg ctggcgagcc ggtctgctcc tgccttcctg tgcagacatt cctcggggga
     2101 cccacttgca aactgttaac caaaagtgcc atcttccaga gcccagagaa cgatggcagt
     2161 gtcctcgtgt gcactgcaga tgaagcgtca cagtctgccc tggtgtggaa tgctggcagt
     2221 ggctcattgc tgcagaacct gaagactgat gagcctgtct tggacatctg cccttttgaa
     2281 acgaatcaga acagctactt ggccaccttg accgagaaga ttgtccacat ttataaatgg
     2341 gagtgagcgt ggccgtgaga ccgctaggca cgccgctagt taatgtggca tagtctggag
     2401 cacctcatga ctccaaacaa gatcgctgtc tacatgctgg agctggaact tctggatcat
     2461 ggttcattgg attagtgtga ttgtgtgcgg tttacaccaa atgagtacct ttgtgaatct
     2521 tgcctggaaa ttttttactt aattctatag gatcttacag tcctctcaac taccatttag
     2581 gatatgagtg cacaccaagc atttgggggt tgtaatagcc aacctaaatt gtcgtgtcag
     2641 ttaggctaat tggcttaaaa gcagcacact acaggcctgc catgcttctt ggatgcaaaa
     2701 gataggcggg cgagtcttcc attaagcaca aagggcactg ttcatatcct gtgtaggatt
     2761 tgacctattt ccaagtctga atgcttacaa ccttttagcg actatttcgc ccagacccct
     2821 gcactgcttg ctgcccccaa tgtctggtac ttctgtgtgg tgtgctttgt ggcaacaagt
     2881 tccatcccag gcatccaggc cgactcaaat gttggcaagg ctggtggtgg aggtggctga
     2941 tgagccgcca tatgcaaaac catatcaagc cccgttgcat tgtgggaggt aaggaaaggg
     3001 accgggtggt tgggattttt ctggttagct tgggatgctg ggggggtttt actcccccta
     3061 aagtcaatgc ctaaggatat atagttccca agtaaaggct ccccccaccc cctttttttc
     3121 tgaaaaggtt gttaatgaag tggttatgct caggaaggta acagtggcta gagatctgtg
     3181 ggaggcagga aggaagactc ttagaaactt ccgtcatggc ggtcctgcac agtccagcat
     3241 ctagaggttt gctcgggttt gtctacttgg ccttaggcta ccccactgag ccactgccct
     3301 acaacacctt gtctcaaggt gtggccaacc tgtctgattt ctctcagatg ttccttgatg
     3361 aagcactatc ttttttaata aaaattcttt catgcctgtc aaactcttga gctgttttgg
     3421 tgatctttat tgccaacaag ggattgttaa gggaaaattg ttaattttcc cagggttcca
     3481 agttgtggct gtgtctgctt aatgaaacct tctacttgaa gatgtatagt ttgttttgtt
     3541 tttaaaactt aaagctttta aaatgtcagc ttccagccag ttgaagtggt gaatgccttt
     3601 tgccccagca ctctgaaggc agaaacagat ggatcccctg tgagttcaag gccaccctca
     3661 ctacaaggtg agttttggac agccaaaagc tagaacacag agaaaccctg tctcaaaaaa
     3721 aaaaaggggg gggcaaaaga aaaatgtcag cttccacagc ttggagaaaa gtcacctttt
     3781 ctccctctct ccccccagat gcttttgata gattgatggt taagggtaaa tagaattcac
     3841 ctaacttctt caggtggcca cagaaagagc tgtgggtgtc aggttgatct ggagcaggct
     3901 taggatttga cttgcgttga aggacattta actaaggcca actgggggga tttgctacca
     3961 cagaagctaa cctaaatacc aagccttgac agcttctgca tttctgcaat ttgaattcgg
     4021 gtaggggagt gccaggacct caacctggtt aagttttttg ctctactact gagctgtact
     4081 agaaacccaa atctgtgttt tggaagcaga ttcccattat agactgattc tccaaagaca
     4141 gccttacccc tacttgcaag aaacttcatc cctaacaccc aggcaccagc aggatctgtt
     4201 tttaaatctt gtcacgggac attgggattt ccgaatattt gctgtgctgc atttcactgt
     4261 ttgttttaat gtcaacaaaa taaagaatct gtaatgtgta accccaa
//
