LOCUS       NM_001402201            4304 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus ring finger and WD repeat domain 3 (Rfwd3),
            transcript variant 1, mRNA.
ACCESSION   NM_001402201 XM_039098247
VERSION     NM_001402201.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Knies,K., Inano,S., Ramirez,M.J., Ishiai,M., Surralles,J.,
            Takata,M. and Schindler,D.
  TITLE     Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi
            anemia
  JOURNAL   J Clin Invest 127 (8), 3013-3027 (2017)
   PUBMED   28691929
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Inano,S., Sato,K., Katsuki,Y., Kobayashi,W., Tanaka,H.,
            Nakajima,K., Nakada,S., Miyoshi,H., Knies,K., Takaori-Kondo,A.,
            Schindler,D., Ishiai,M., Kurumizaka,H. and Takata,M.
  TITLE     RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA
            and RAD51 from DNA Damage Sites to Facilitate Homologous
            Recombination
  JOURNAL   Mol Cell 66 (5), 622-634 (2017)
   PUBMED   28575658
  REMARK    Erratum:[Mol Cell. 2020 Apr 2;78(1):192. PMID: 32243829]
REFERENCE   3  (bases 1 to 4304)
  AUTHORS   Feeney,L., Munoz,I.M., Lachaud,C., Toth,R., Appleton,P.L.,
            Schindler,D. and Rouse,J.
  TITLE     RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for
            Interstrand Crosslink Repair and Human Health
  JOURNAL   Mol Cell 66 (5), 610-621 (2017)
   PUBMED   28575657
REFERENCE   4  (bases 1 to 4304)
  AUTHORS   Elia,A.E., Wang,D.C., Willis,N.A., Boardman,A.P., Hajdu,I.,
            Adeyemi,R.O., Lowry,E., Gygi,S.P., Scully,R. and Elledge,S.J.
  TITLE     RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled
            Replication Forks
  JOURNAL   Mol Cell 60 (2), 280-293 (2015)
   PUBMED   26474068
REFERENCE   5  (bases 1 to 4304)
  AUTHORS   Gong,Z. and Chen,J.
  TITLE     E3 ligase RFWD3 participates in replication checkpoint control
  JOURNAL   J Biol Chem 286 (25), 22308-22313 (2011)
   PUBMED   21504906
REFERENCE   6  (bases 1 to 4304)
  AUTHORS   Fu,X., Yucer,N., Liu,S., Li,M., Yi,P., Mu,J.J., Yang,T., Chu,J.,
            Jung,S.Y., O'Malley,B.W., Gu,W., Qin,J. and Wang,Y.
  TITLE     RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53
            stability in response to DNA damage
  JOURNAL   Proc Natl Acad Sci U S A 107 (10), 4579-4584 (2010)
   PUBMED   20173098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Mar 8, 2022 this sequence version replaced XM_039098247.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360172.775804.1,
                                           SRR26360181.1784658.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                JAXUCZ010000019.1  56177746-56177815   c
            71-590              JAXUCZ010000019.1  56174759-56175278   c
            591-757             JAXUCZ010000019.1  56170907-56171073   c
            758-825             JAXUCZ010000019.1  56167311-56167378   c
            826-1008            JAXUCZ010000019.1  56164797-56164979   c
            1009-1100           JAXUCZ010000019.1  56164620-56164711   c
            1101-1215           JAXUCZ010000019.1  56159284-56159398   c
            1216-1444           JAXUCZ010000019.1  56158347-56158575   c
            1445-1595           JAXUCZ010000019.1  56155999-56156149   c
            1596-1772           JAXUCZ010000019.1  56152247-56152423   c
            1773-1987           JAXUCZ010000019.1  56150343-56150557   c
            1988-2199           JAXUCZ010000019.1  56148700-56148911   c
            2200-4304           JAXUCZ010000019.1  56145221-56147325   c
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..4304
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /note="ring finger and WD repeat domain 3"
                     /db_xref="GeneID:103690025"
                     /db_xref="RGD:1305243"
     exon            1..70
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..2343
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase RFWD3-like"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RFWD3 isoform 1"
                     /protein_id="NP_001389130.1"
                     /db_xref="GeneID:103690025"
                     /db_xref="RGD:1305243"
                     /translation="MSDLVPAVVAHEAMDCGLQVPLDGTLEQAAPTEVISHGAPLLQP
                     PPSDLVSSQGEALVQPAPADVVSSQGGLPTLQPVPPASIDLTEEVQPAEENMEIVDPG
                     TSEERSEGSGTNGAVGAAPSVSMNYFISGLQRLHTMLELLRPSSSQSDHSAGGPIRRR
                     RRTGFVSRARTGGSQRLNNARLRTFFQVSRSQPYPVASAHGSEARTPPSGTDSDSDSS
                     AENEEAAARAEEPEAAVPEQGVISTDQEAASVTGGDASPKKSPQKPIVLPTVEDEEGE
                     TCTICLEQWTSAGDHRLSALRCGHLFGYRCIFKWLKGQTRKCPQCNKKAKHSDIVVLY
                     ARSLRALDTTEQERMKSDLLSEQALRKQAELESAQCRLQLQVLMDKCTKLNSRVQDLE
                     KLMVRHQGPAGQQCSGSQALCGSCLPSSQSQRKYSSEKTFTVSQTGNCRIMAYCDGLS
                     CLVASQPSPQASFLPGFGVKMLSTANMRSSQYIPMHSKQIRGLAFSNQSKGLLLSASL
                     DNTIKLTSLETNTVVQTYNTGRPVWSCCWCLDENNYIYAGLANGSILIYDLRNTNTYV
                     QELVPQKARCPLVSLSYLPRAAVTAFPYGGVLAGTLENASFWELKTDFCHKPHVLSLE
                     PGGFVDFQTESSTRHCLVTYRPDKSHNTVRTVLLEMSYRLDDAGEPVCSCLPVQTFLG
                     GPTCKLLTKSAIFQSPENDGSVLVCTADEASQSALVWNAGSGSLLQNLKTDEPVLDIC
                     PFETNQNSYLATLTEKIVHIYKWE"
     exon            71..590
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            591..757
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            758..825
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1008
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1100
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1215
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1444
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1595
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1772
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1987
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2199
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..4304
                     /gene="Rfwd3"
                     /gene_synonym="RGD1305243"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgcgcggta ggttttgcgc ggtagcgacg gcagaagtcc cgctcgggat gtccgacttg
       61 gtccccgcag tggtggctca tgaagcaatg gactgcggtc ttcaggtacc attagacggt
      121 actctagaac aagccgcccc aactgaagtc atcagccatg gggcaccact tctccagcct
      181 ccgccttctg acttggtcag cagccaaggc gaagcgcttg ttcagcctgc ccctgctgac
      241 gtggtcagca gccaaggcgg actgcccact ctgcagcctg tgccaccagc atccattgat
      301 ttgacggagg aagtacagcc cgcagaggag aatatggaga ttgtcgatcc gggaacttcg
      361 gaggagcgca gtgaggggtc aggcactaac ggtgccgtcg gagcggctcc atcagtttca
      421 atgaactact tcatcagcgg gctgcagagg cttcatacca tgctggagtt gctgagacct
      481 tcatcgtcac agtcagacca cagtgcgggg gggccaataa gaaggaggag aagaacagga
      541 ttcgtttcga gggcaagaac tggagggtct cagaggctaa acaatgccag gttgagaacc
      601 ttctttcagg tgagcaggtc tcagccttac ccagtagcct ctgctcatgg ttcagaggcc
      661 aggaccccac cctcgggaac tgattccgac agcgacagct ctgcagagaa tgaagaggcc
      721 gctgcccggg cggaagagcc tgaggctgct gtcccagaac aaggagttat ctcaacagac
      781 caagaagctg catctgtcac aggaggtgac gcatccccta aaaagtctcc ccagaagccc
      841 atcgttctac ccactgtgga agatgaggaa ggggaaactt gcacgatctg tttggaacag
      901 tggaccagtg ctggggacca ccggctctca gctttacgct gcgggcacct ctttggatat
      961 agatgcattt ttaaatggct caaaggacaa acacgaaaat gtcctcagtg caacaagaaa
     1021 gccaagcaca gtgacatcgt tgtcctttat gcccggtccc tgagagcttt ggacactact
     1081 gaacaggagc gcatgaagag tgacctactg agtgagcagg ctctccggaa gcaggccgag
     1141 ctggaatccg cccagtgccg gctccagctt caggtcctta tggataaatg cacgaaactc
     1201 aatagccgtg tccaggactt ggagaagctt atggtgcggc atcagggccc agcaggacag
     1261 cagtgcagcg gctcccaagc actttgtggg agctgcctgc cctccagcca gagccagcgc
     1321 aaatacagct cagagaagac cttcacagta tctcagacgg gaaactgccg aatcatggcg
     1381 tattgcgatg gtctgagctg cttggtggca tcccagccct ctcctcaggc ttccttcctt
     1441 ccaggctttg gtgttaagat gctgagtact gcgaacatga gaagcagtca gtacatccct
     1501 atgcatagca agcagattcg tggactagct ttcagcaatc aatccaaagg cttgctcctc
     1561 tccgcctccc ttgacaacac cattaaactg accagcctag aaacaaatac tgtggtgcag
     1621 acttacaaca ctggacgccc tgtctggagc tgttgctggt gtcttgacga aaacaattac
     1681 atctacgctg gactggccaa tggttcaatt ctgatctatg acttacggaa cacaaatacc
     1741 tatgtccagg aattagttcc tcagaaagcc agatgtcctc tggtgtccct gtcatattta
     1801 cccagagctg cggtgactgc atttccttac ggtggggtgc tggctggaac cttggagaat
     1861 gcctccttct gggagctaaa aacagacttt tgtcataagc ctcatgtgct atccttggag
     1921 ccagggggct ttgtagactt tcagacagag agcagcaccc gacactgtct tgtgacatac
     1981 aggcctgata aaagccataa caccgtgcga actgtgctgt tggagatgtc ttacagactg
     2041 gatgacgctg gcgagccggt ctgctcctgc cttcctgtgc agacattcct cgggggaccc
     2101 acttgcaaac tgttaaccaa aagtgccatc ttccagagcc cagagaacga tggcagtgtc
     2161 ctcgtgtgca ctgcagatga agcgtcacag tctgccctgg tgtggaatgc tggcagtggc
     2221 tcattgctgc agaacctgaa gactgatgag cctgtcttgg acatctgccc ttttgaaacg
     2281 aatcagaaca gctacttggc caccttgacc gagaagattg tccacattta taaatgggag
     2341 tgagcgtggc cgtgagaccg ctaggcacgc cgctagttaa tgtggcatag tctggagcac
     2401 ctcatgactc caaacaagat cgctgtctac atgctggagc tggaacttct ggatcatggt
     2461 tcattggatt agtgtgattg tgtgcggttt acaccaaatg agtacctttg tgaatcttgc
     2521 ctggaaattt tttacttaat tctataggat cttacagtcc tctcaactac catttaggat
     2581 atgagtgcac accaagcatt tgggggttgt aatagccaac ctaaattgtc gtgtcagtta
     2641 ggctaattgg cttaaaagca gcacactaca ggcctgccat gcttcttgga tgcaaaagat
     2701 aggcgggcga gtcttccatt aagcacaaag ggcactgttc atatcctgtg taggatttga
     2761 cctatttcca agtctgaatg cttacaacct tttagcgact atttcgccca gacccctgca
     2821 ctgcttgctg cccccaatgt ctggtacttc tgtgtggtgt gctttgtggc aacaagttcc
     2881 atcccaggca tccaggccga ctcaaatgtt ggcaaggctg gtggtggagg tggctgatga
     2941 gccgccatat gcaaaaccat atcaagcccc gttgcattgt gggaggtaag gaaagggacc
     3001 gggtggttgg gatttttctg gttagcttgg gatgctgggg gggttttact ccccctaaag
     3061 tcaatgccta aggatatata gttcccaagt aaaggctccc cccaccccct ttttttctga
     3121 aaaggttgtt aatgaagtgg ttatgctcag gaaggtaaca gtggctagag atctgtggga
     3181 ggcaggaagg aagactctta gaaacttccg tcatggcggt cctgcacagt ccagcatcta
     3241 gaggtttgct cgggtttgtc tacttggcct taggctaccc cactgagcca ctgccctaca
     3301 acaccttgtc tcaaggtgtg gccaacctgt ctgatttctc tcagatgttc cttgatgaag
     3361 cactatcttt tttaataaaa attctttcat gcctgtcaaa ctcttgagct gttttggtga
     3421 tctttattgc caacaaggga ttgttaaggg aaaattgtta attttcccag ggttccaagt
     3481 tgtggctgtg tctgcttaat gaaaccttct acttgaagat gtatagtttg ttttgttttt
     3541 aaaacttaaa gcttttaaaa tgtcagcttc cagccagttg aagtggtgaa tgccttttgc
     3601 cccagcactc tgaaggcaga aacagatgga tcccctgtga gttcaaggcc accctcacta
     3661 caaggtgagt tttggacagc caaaagctag aacacagaga aaccctgtct caaaaaaaaa
     3721 aagggggggg caaaagaaaa atgtcagctt ccacagcttg gagaaaagtc accttttctc
     3781 cctctctccc cccagatgct tttgatagat tgatggttaa gggtaaatag aattcaccta
     3841 acttcttcag gtggccacag aaagagctgt gggtgtcagg ttgatctgga gcaggcttag
     3901 gatttgactt gcgttgaagg acatttaact aaggccaact ggggggattt gctaccacag
     3961 aagctaacct aaataccaag ccttgacagc ttctgcattt ctgcaatttg aattcgggta
     4021 ggggagtgcc aggacctcaa cctggttaag ttttttgctc tactactgag ctgtactaga
     4081 aacccaaatc tgtgttttgg aagcagattc ccattataga ctgattctcc aaagacagcc
     4141 ttacccctac ttgcaagaaa cttcatccct aacacccagg caccagcagg atctgttttt
     4201 aaatcttgtc acgggacatt gggatttccg aatatttgct gtgctgcatt tcactgtttg
     4261 ttttaatgtc aacaaaataa agaatctgta atgtgtaacc ccaa
//
