LOCUS       NM_001170531            4226 bp    mRNA    linear   ROD 03-JUL-2024
DEFINITION  Rattus norvegicus RAS protein-specific guanine nucleotide-releasing
            factor 1 (Rasgrf1), transcript variant 1, mRNA.
ACCESSION   NM_001170531
VERSION     NM_001170531.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abarzua,S., Ampuero,E. and van Zundert,B.
  TITLE     Superoxide generation via the NR2B-NMDAR/RasGRF1/NOX2 pathway
            promotes dendritogenesis
  JOURNAL   J Cell Physiol 234 (12), 22985-22995 (2019)
   PUBMED   31245854
  REMARK    GeneRIF: our data indicate that superoxide production is induced by
            the NR2B-NMDARs/RasGRF1/NOX2 pathway and promotes dendritogenesis.
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Wu,R.F., Liao,C., Hatoum,H., Fu,G., Ochoa,C.D. and Terada,L.S.
  TITLE     RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to
            Ras
  JOURNAL   Arterioscler Thromb Vasc Biol 37 (1), 98-107 (2017)
   PUBMED   27856453
REFERENCE   3  (bases 1 to 4226)
  AUTHORS   Song,J.M., Sung,Y.M., Nam,J.H., Yoon,H., Chung,A., Moffat,E.,
            Jung,M., Pak,D.T., Kim,J. and Hoe,H.S.
  TITLE     A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor
            Increases Dendritic Spine Density via RasGRF1/ERK Pathway
  JOURNAL   J Alzheimers Dis 51 (2), 591-604 (2016)
   PUBMED   26890742
  REMARK    GeneRIF: Novel mercaptoacetamide-based class II histone deacetylase
            inhibitor HDACIW2 treatment regulated the levels of RasGRF1 and
            p-ERK, and that W2 requires RasGRF1 and ERK signaling to alter
            dendritic spine formation, suggesting that W2 regulates dendritic
            spine formation via a RasGRF1/ERK-dependent signaling pathway
REFERENCE   4  (bases 1 to 4226)
  AUTHORS   DiBattista,A.M., Dumanis,S.B., Song,J.M., Bu,G., Weeber,E.,
            Rebeck,G.W. and Hoe,H.S.
  TITLE     Very low density lipoprotein receptor regulates dendritic spine
            formation in a RasGRF1/CaMKII dependent manner
  JOURNAL   Biochim Biophys Acta 1853 (5), 904-917 (2015)
   PUBMED   25644714
  REMARK    GeneRIF: VLDLR requires RasGRF1/CaMKII to alter dendritic spine
            formation.
REFERENCE   5  (bases 1 to 4226)
  AUTHORS   Oishi,M., Yamashiro,K., Miyake,M., Akagi-Kurashige,Y., Kumagai,K.,
            Nakata,I., Nakanishi,H., Yoshikawa,M., Oishi,A., Gotoh,N.,
            Tsujikawa,A., Yamada,R., Matsuda,F. and Yoshimura,N.
  CONSRTM   Nagahama Study Group
  TITLE     Association between ZIC2, RASGRF1, and SHISA6 genes and high myopia
            in Japanese subjects
  JOURNAL   Invest Ophthalmol Vis Sci 54 (12), 7492-7497 (2013)
   PUBMED   24150758
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4226)
  AUTHORS   MacDonald,J.I., Verdi,J.M. and Meakin,S.O.
  TITLE     Activity-dependent interaction of the intracellular domain of rat
            trkA with intermediate filament proteins, the beta-6 proteasomal
            subunit, Ras-GRF1, and the p162 subunit of eIF3
  JOURNAL   J Mol Neurosci 13 (1-2), 141-158 (1999)
   PUBMED   10691301
REFERENCE   7  (bases 1 to 4226)
  AUTHORS   Anborgh,P.H., Qian,X., Papageorge,A.G., Vass,W.C., DeClue,J.E. and
            Lowy,D.R.
  TITLE     Ras-specific exchange factor GRF: oligomerization through its Dbl
            homology domain and calcium-dependent activation of Raf
  JOURNAL   Mol Cell Biol 19 (7), 4611-4622 (1999)
   PUBMED   10373510
REFERENCE   8  (bases 1 to 4226)
  AUTHORS   Pearsall,R.S., Plass,C., Romano,M.A., Garrick,M.D., Shibata,H.,
            Hayashizaki,Y. and Held,W.A.
  TITLE     A direct repeat sequence at the Rasgrf1 locus and imprinted
            expression
  JOURNAL   Genomics 55 (2), 194-201 (1999)
   PUBMED   9933566
REFERENCE   9  (bases 1 to 4226)
  AUTHORS   Tung,P.S., Fam,N.P., Chen,L. and Moran,M.F.
  TITLE     A 54-kDa protein related to ras-guanine nucleotide release factor
            expressed in the rat exocrine pancreas
  JOURNAL   Cell Tissue Res 289 (3), 505-515 (1997)
   PUBMED   9232829
REFERENCE   10 (bases 1 to 4226)
  AUTHORS   Shou,C., Farnsworth,C.L., Neel,B.G. and Feig,L.A.
  TITLE     Molecular cloning of cDNAs encoding a guanine-nucleotide-releasing
            factor for Ras p21
  JOURNAL   Nature 358 (6384), 351-354 (1992)
   PUBMED   1379346
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB713048.1, CB607130.1, CB714920.1, BF405809.1, CB583610.1 and
            BC091317.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMEA5760396 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               CB713048.1         13-149
            138-640             CB607130.1         13-515
            641-725             CB714920.1         277-361
            726-771             BF405809.1         243-288             c
            772-820             CB714920.1         408-456
            821-1350            CB583610.1         12-541
            1351-4226           BC091317.1         1-2876
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q31"
     gene            1..4226
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /note="RAS protein-specific guanine nucleotide-releasing
                     factor 1"
                     /db_xref="GeneID:192213"
                     /db_xref="RGD:620395"
     exon            1..403
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..3865
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ras-specific guanine nucleotide-releasing factor 1;
                     guanine nucleotide-releasing protein"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     1 isoform 1"
                     /protein_id="NP_001164002.1"
                     /db_xref="GeneID:192213"
                     /db_xref="RGD:620395"
                     /translation="MQKAIRLNDGHVVSLGLLAQRDGTRKGYLSKRSSDNPKWQTKWF
                     ALLQNLLFYFESDSSSRPSGLYLLEGSICKRMPSPKRGTSSKESDKQHHYFTVNFSND
                     SQKSLELRTDDSKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQL
                     RQQLEDGEVEIERLKAEIANLIKDNERIQSNQLVAPEDEDSDIKKIKKVQSFLRGWLC
                     RRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASS
                     KKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQ
                     EFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTL
                     HELLAHTPHEHVERNSLDYAKSKLEELSRVMHDEVSETENIRKNLAIERMITEGCEIL
                     LDTSQTFVRQGSLIQVPMSEKGKINKGRLGSLSLKKEGERQCFLFSKHLIICTRGSGS
                     KLHLTKNGVISLIDCTLLDDPENMDDDGKGQEVDHLDFKIWVEPKDSPPFTVILVASS
                     RQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKT
                     MNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTDAVVVLDKLISIYKK
                     PITAIPARSLELLFSSSHNTKLLYGDAPKSPRASRKFSSPPPLAIGTSSPVRRRKLSL
                     NIPIITGGKALELASLGCPSDGYTNIHSPISPFGKTTLDTSKLCVASSLTRTPEEIDM
                     TTLEESSGFRKPTSDILKEESDDDQSDVDDTEVSPPTPKSFRNRITQEFPLFNYNSGI
                     MMTCRDLMDSNRSPLSATSAFAIATAGANESPANKEIYRRMSLANTGYSSDQRNIDKE
                     FVIRRAATNRVLNVLRHWVTKHSQDFETDDLLKYKVICFLEEVMHDPDLLPQERKAAA
                     NIMRTLTQEEITENHSMLDELLLMTEGVKTEPFENHSAMEIAEQLTLLDHLVFKSIPY
                     EEFFGQGWMKADKNERTPYIMKTTRHFNHISNLIASEILRNEEVSARASTIEKWVAVA
                     DICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLFDKLQKLVSSDGRFKNL
                     RETLRNCDPPCVPYLGMYLTDLAFLEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQ
                     TTYKIEPQPKVTQYLVDETFVLDDESLYEASLRIEPKLPT"
     exon            404..513
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            514..661
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            662..754
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            755..1008
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1088
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1282
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1392
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1511
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1672
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1736
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1867
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1950
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2199
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2561
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2666
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2837
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2944
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3057
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3058..3118
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3119..3222
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3223..3307
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3308..3505
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3506..3585
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3586..3703
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3704..3772
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
     exon            3773..4189
                     /gene="Rasgrf1"
                     /gene_synonym="GNRP; P140 RAS-GRF"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagagagcga gagagagagg gagcgttcag cgagcgccag agaacggcag agcggcgcgc
       61 accgacgagg ggcagtcggg tgcctctcgg agatgtttag tgcgtgaggt ctctctggcc
      121 tccaagcacc atgcagaaag ccatccgact gaacgatggc cacgtcgtgt ccctgggact
      181 gctggcccag agagacggca cgcgcaaagg ctacctgagc aagaggagtt cggacaaccc
      241 aaaatggcaa accaagtggt ttgcgctgct gcagaacctg ctcttctact tcgaaagtga
      301 ctcgagctct cggccctcgg ggctctacct gctggagggc agtatctgca aacgcatgcc
      361 ctcccccaag cgagggacct cctccaagga gtccgacaaa cagcatcatt acttcacagt
      421 gaacttctcc aatgacagcc agaagtccct agagctgagg accgatgact ccaaggactg
      481 tgacgagtgg gtggcagcga ttgctcgcgc cagctacaag atactggcca cagagcatga
      541 ggcgctcatg cagaagtacc tgcacctgct gcaggtggtg gagacagaga agaccgtggc
      601 taagcagctg cgacagcagc tcgaggatgg cgaggtcgag atcgagcgcc tgaaggcaga
      661 gattgcaaac ctgatcaagg acaatgaacg tatccagtcc aaccagctgg ttgcccctga
      721 ggatgaggac agtgacatca agaaaattaa gaaggtacag agtttccttc gcggatggct
      781 gtgccggcga aagtggaaga acatcatcca ggactacatc cggtctcctc atgccgacag
      841 catgcgcaag aggaaccagg tggtgttcag catgctggaa gctgaggccg agtacgtgca
      901 gcaactacac atccttgtca acaattttct gcgcccactg cgcatggccg ccagctctaa
      961 gaaaccccct ataacacatg acgacgtcag cagtatcttt ctgaacagtg agaccatcat
     1021 gttcctgcac cagatcttct accaaggcct gaaggcccgt atcgccagct ggcccaccct
     1081 ggttctggcg gacctgttcg acatcctgct gccaatgctt aacatctacc aggagttcgt
     1141 ccgcaaccac cagtacagtc tccagatcct agcacactgc aagcaaaacc gggactttga
     1201 caagctcctc aagcagtatg aggccaagcc agactgcgag gagcgcacac tggagacctt
     1261 cctcacctat ccaatgttcc agatccccag gtacatcctg acactccatg agctgctggc
     1321 ccacacacct catgagcatg tggagcgcaa cagcctggac tatgccaaat ccaaactaga
     1381 ggagctgtcc agggtcatgc acgacgaagt cagtgagacc gagaacatcc gcaaaaacct
     1441 ggccattgag cgtatgatca ccgagggctg tgagatcctc cttgacacca gccagacctt
     1501 tgtgcgccaa ggttccctca tccaggtgcc catgtcagaa aagggcaaga tcaacaaggg
     1561 ccgcctgggg tctctgtccc ttaagaaaga aggtgagcgc cagtgtttcc tgttctccaa
     1621 gcatctcatc atctgcacca gaggctctgg tagcaaactg cacctaacca agaatggcgt
     1681 gatttccctc attgactgca ctctactgga tgatccagaa aacatggatg atgacggcaa
     1741 aggacaagag gtagatcacc tggactttaa gatttgggtg gagccaaagg attccccacc
     1801 cttcacagtc atcctggtgg cctcatccag gcaggagaag gcggcatgga ccagtgacat
     1861 catccagtgc gtggataata tccgctgcaa cgggctcatg atgaatgcct ttgaagaaaa
     1921 ttccaaggtc accgtgccgc agatgatcaa gtctgatgct tccttatact gtgatgatgt
     1981 tgacattcgc ttcagcaaaa ccatgaattc ttgcaaagtg ctgcagatcc gctatgccag
     2041 cgtggagcgc ctgctggagc gcctgactga tcttcgcttc ctgagtattg actttctcaa
     2101 caccttcctg cactcctatc gagtcttcac cgatgctgtg gtggtcctag acaagctgat
     2161 cagcatctac aaaaagccca tcactgcgat tcctgccagg tcactggaac tcctgttctc
     2221 cagtagccac aacaccaaac ttctgtacgg agatgccccc aagtcgcctc gtgccagccg
     2281 caagttctcc tcgccgccgc ccttggccat cggcacttcg tccccagtcc gccgccggaa
     2341 gttgtctctc aacattccca tcatcacagg cggcaaggcg ctggaactgg cttcgctcgg
     2401 gtgcccctcc gacggctaca ccaacataca ctcgcccata tctcccttcg gcaaaaccac
     2461 gctggacacc agcaagctct gtgtggccag cagcttgacc agaacgccgg aggagattga
     2521 tatgaccact ctagaggagt catcaggctt caggaagccg acctcagaca tcttgaaaga
     2581 agagtctgat gatgaccaga gtgatgtaga cgacacagaa gtgtctccac caacaccgaa
     2641 atcattcaga aacagaatca ctcaagagtt cccactcttt aactacaaca gtggaatcat
     2701 gatgacatgt cgcgatctga tggacagtaa ccgcagccct ctgtcagcta cctctgcctt
     2761 tgccatagcg actgcaggag ccaatgaaag ccccgcaaac aaggagatat atcgaaggat
     2821 gtctttggcc aacacagggt attcctctga ccagagaaat atcgacaaag agttcgtgat
     2881 ccgcagagcg gccaccaacc gtgtactgaa tgtgttgcgc cactgggtca ccaagcactc
     2941 ccaggacttt gaaactgacg acctcctcaa atacaaggtg atctgctttc tggaagaggt
     3001 catgcatgac ccagaccttc taccacaaga gcgaaaggca gcagccaaca tcatgaggac
     3061 tctgacccag gaagaaataa ctgaaaacca tagcatgctg gatgagctct tactaatgac
     3121 ggagggtgtg aagactgagc ccttcgaaaa ccactcagcc atggagatag cagagcagct
     3181 gaccctgctg gatcaccttg tcttcaagag tattccttat gaggaattct ttggccaggg
     3241 ctggatgaag gcagataaga atgaaaggac accttacatt atgaaaacca ccagacattt
     3301 caaccatatc agtaacttga tcgcttcaga aattctccga aacgaggagg tcagtgcaag
     3361 ggcaagcacc atcgagaagt gggtggctgt tgccgacatt tgccgctgcc tgcacaacta
     3421 caatgctgtg ctggagatca cttcctccat caaccgcagc gcaatcttcc gactcaagaa
     3481 gacatggctc aaagtttcta agcagacgaa atctctgttt gacaagctcc aaaagcttgt
     3541 gtcatcagat ggccgattta agaacctcag agaaactttg cgaaattgtg atccaccctg
     3601 tgtcccttac ctggggatgt acctgaccga cttggcattc ctcgaggaag gaacacccaa
     3661 ttacacagag gacggcctgg tcaacttctc caagatgagg atgatctccc atattatccg
     3721 cgagattcgc cagtttcagc agactactta caaaatcgag ccccagccaa aggtaactca
     3781 gtacttagtg gatgaaacct ttgtgttgga cgacgaaagt ctgtatgagg cctccctccg
     3841 aattgaacca aaactcccca catgaggctg cactccccac ccctgctgca ccgcgccaca
     3901 ttgcgccaca ctgcgctgcg ccgtgccatg ccgtaacccc atgccgtaac cccatgccgt
     3961 aaccccatgc cgtaacccca gccgtaaccc catgccgtaa ccccatgccg taaccccagc
     4021 cgcttctgtg aacacctgct atgtgatgtt gtagaataac catgtaatgt tgtagaagaa
     4081 ccgttcgtgt tcctctgtac ttgttctgcg gtgtgcgcct ctctgagaat atataatctg
     4141 ttcctgtcgt gtaaacccga agaataaatg tctgcattca aaatacaaaa aaaaaaaaaa
     4201 aaaaaaaaaa aaaaaaaaaa aaaaaa
//
